LOCUS       gnlProkkaGNGKBCJN_1    14265 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GNGKBCJN_1.
ACCESSION   gnlProkkaGNGKBCJN_1
VERSION     gnlProkkaGNGKBCJN_1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-16 23:33:45
            Original ID  :: gnl|Prokka|GNGKBCJN_1
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 2151202
            Orig. end    :: 2165467
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14265
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:9265]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..9265
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..14265
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..14265
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="phosphonate"
                     /region_number="2"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             complement(494..1297)
                     /locus_tag="ctg1_1746"
                     /transl_table=11
                     /translation="MATTYDGINYFPIAVNFMEENAMEVIEAKYGMKGLAIVLKLLCKI
                     YKEGYYIPWGEEQCLIFANKTGKEADAEEVQGIIEIMIEKGMIDRGSYAEHKVLTSEAI
                     QKVWIEATKRRKRNWTAMPYLLIKPKETCNEEKIVCTQNVEQDAGSDAKNACNTEQSKV
                     KQSREFPPSAPPRGKEEEVNATPVSMPGYAFNTMTHNYVGLMGNLERFGITDEKEIEAI
                     LRLSDYGRKGTPVWKLICSTNWSNIGAKGKYMIAALNRAKKRSGT"
     CDS             complement(1347..1694)
                     /locus_tag="ctg1_1747"
                     /transl_table=11
                     /translation="MKKNRKAVSKNEMMKGKWKRQIMLEKDYTEQCSEWMAERLEALIE
                     YMQYGHAAVAYMKQDGTFKLVKGTLVGYEKDFGKQYDPMEIKNTVVYRDVEQQRWMTFK
                     IENFMEWRAIV"
     CDS             complement(1834..2172)
                     /locus_tag="ctg1_1748"
                     /transl_table=11
                     /translation="MNDNLVTIEVNGKVIFAGKADLQFSLNQKVREPLRITKGKGKLME
                     ALTESFIKGGINSMENRPIEELQETIKEYLTFEYQRKGIATEPNKRSFLSELKKYARAF
                     RKKRDESE"
     CDS             2694..3218
                     /locus_tag="ctg1_1749"
                     /transl_table=11
                     /translation="MSEQQKYWFAARTRDKQEFAIRDSLEKLKTELDLNYYLPTQFVIR
                     QLKYRRKRVEVPVIKNLIFIQATKQDACDISNKYNIQLFYMKDLLTRAMLIVPDKQMQD
                     FIFVMDLDPNGVSFDNDHLSVGSRVQVVKGDFCGVEGELASEANKTYVVIRIAGVLSAS
                     VKVPKSYLRVI"
     CDS             3222..3707
                     /locus_tag="ctg1_1750"
                     /transl_table=11
                     /translation="MNTLTSQIEQLQSLAHELLYLGVDGAPIYTDHFRQLNKEVLEQSD
                     ALYPQRGATPEEEANICLALLMGYNATIYNQGDKEEKKQVVLNRCWDVLDQLPATLLKC
                     QLLTYCYGEVFEEELAKEAHTIIESWSNRELLKAEKEIAESLNNLEANPYPYSELHE"
     CDS             3704..4987
                     /locus_tag="ctg1_1751"
                     /transl_table=11
                     /translation="MIRSFLSRSPLYVLALYASVLIFLVYTCAYAYRKPFTAAIYEGEI
                     LWGFDVKILYVLSEIIGYALSKFIGVRILPSMKAGHRIYYIIGLLTFSEVALLGFALLP
                     VPLKVCSIFLSGLPLGMIWGVIFSYIEGRRISEILNVGLSVALIVSSGLVKTLGQFVMD
                     NLHVSEYWMPFTTGLLVYPVMLLCSWLLNQIPAPNEQDIIQRTKRAPMDGKDRRKFLRQ
                     FFWGICMLVVFYGALTVFRELRDSFAADIWKELHIEGAMIFTQTEVPIAVFVLVLMFLI
                     VFVRNNRLALNIIYCIAVTGGSLMVFSTLLYVYGFLSPIWWMILSGLGLYMGYIPFTYL
                     IERLIASLRVVSTAVFIIYLADSFGYLGTTGVFMVKNFVSADISWTVMLMRTALFSGFV
                     SVLSIFAIYCYFKQQLNSLILIPSEIHD"
     CDS             5001..5699
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_1752"
                     /sec_met_domain="NTP_transf_3 (E-value: 4.6e-12, bitscore:
                     44.5, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAGLGTRFGHYTELVPKGFVEVGGKPMIIRSIETLLSCGIERII
                     LGTGYKKEAYEALQADFPQIETCFSPRYADTNSMYTLYNTRDVIGDDSFLLLESDLVFE
                     RKAILSLLDDEFPDVMLVSSLTKFQDQYYVEYDCNHILTSCSVDKDALEAKGELVGIHK
                     LSNTFYRRMCADYATILESQPKLGYEYELLRMSRSVSPVRVLRVEGLKWYEIDDEADLS
                     YAEEHIIRYC"
     CDS             5719..7020
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 214.1;
                     E-value: 3.9e-65)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_1753"
                     /sec_met_domain="CTP_transf_like (E-value: 1.1e-10,
                     bitscore: 39.9, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 4e-120, bitscore:
                     398.2, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 2e-62, bitscore:
                     208.8, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKKIYVGMTADIMHPGLIHIIHEATKYGDVIVGLLTDKAIAEHK
                     RLPYLTYDQRKEVVENIKGVCEVVPQEDWSYVPNLEKLKPDYIIHGDDWKTGPLREVRE
                     QVFEVMNAQGGKVIEIPYTRGINSSSLDKDIKAIGTTPDVRLKSLRRLIQAKPVVRILE
                     AHDGLCGLIIENLEVQKGDKREVFDGMWSSSLTDSTSKGKPDIEAVDLTTRLQDLNNIL
                     ECTTKPIIFDGDTGGKIEHFVFTVRTLERHGISAVIIEDKVGLKKNSLFGTDAIQTQDS
                     IEGFCDKIRAGKNAQVTGDFMIIARIESLIAGKPMSDALERAYAYVQAGADGIMIHSKD
                     KSGEDIREFCRTFRKEYAHVPIVVVPTTYDHVHESELHEWGANVVIYANHLLRAAYPAM
                     MNVARTILENERAEEVRPLCMPIKEILELIPGTK"
     CDS             7032..8168
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 70.6;
                     E-value: 1.9e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_1754"
                     /sec_met_domain="TPP_enzyme_N (E-value: 6.6e-20, bitscore:
                     69.4, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.2e-18, bitscore:
                     64.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVSVEKIYDTFLHHGVDFFTGVPDSLLKNICAYITDHAPRGKHII
                     AANEGSAVGIACGYYMASGNVPLVYMQNSGLGNTVNPLLSLADEKVYSLPLLLLVGWRG
                     EPGTKDEPQHKKQGEVTLDLLKAMKIPFIILDTDEDEALAQIHDAVVSAKSRSIPHAIV
                     IRKDTFGKYKLRQEALNDYPLSREEALMQVVDHLGDNDIVVSTTGKLSRELFEYRESKG
                     QGHARDFLTVGSMGHSSSIALGIALEKPDRRVYCLDGDGAFIMHMGAVSNIGDLSPKNY
                     RHVLFNNGAHESVGGQPTLGFRLDIASIAKGCGYAHVLTASDKEGLSCALEKLSGLSGP
                     VLLEIKVRIDSRDDLGRPTTTPVENKEHFMAFLKDTHS"
     CDS             8165..9265
                     /NRPS_PKS="Domain: Aminotran_5 (30-293). E-value: 2.6e-33.
                     Score: 107.3. Matches aSDomain:
                     nrpspksdomains_ctg1_1755_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 79.2; E-value:
                     4.7e-24)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_1755"
                     /sec_met_domain="Aminotran_5 (E-value: 1.2e-28, bitscore:
                     98.1, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIERKILLNPGPATTTDTVKLAQVVPDICPREKEFAGMMKQLRD
                     DLVRVAHGDLSKHTAVLFCGSGTINIDICLNSLLPADKKVLVVNNGAYSTRAVEVCEYY
                     GLPHIDLKFSVYELPDLSVIEKTLSDNPDIALVYTTHHETGTGILNPIREIGKIAHSHD
                     AVFVVDTTSSLGMIPFDIGKDNVDFCMASAQKGLQAMTGLSFIVGDESLIRASRDYPKR
                     SYYCNLYLQYDYFEKTGEMHFTPPVQTVYAARQALDEYFAVGEEAKFARHKRVFEAIHK
                     GLQELGLQYAIKREWQSGLVVSVLYPDDPLWDFDKVHDYCYERGFTIYPGKISTTNTFR
                     LCALGEIDVEDIERFFEVMREAVLKY"
     aSDomain        8255..9043
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg1_1755_Aminotran_5.1"
                     /evalue="2.60E-33"
                     /label="ctg1_1755_Aminotran_5.1"
                     /locus_tag="ctg1_1755"
                     /protein_end="293"
                     /protein_start="30"
                     /score="107.3"
                     /tool="antismash"
                     /translation="PREKEFAGMMKQLRDDLVRVAHGDLSKHTAVLFCGSGTINIDICL
                     NSLLPADKKVLVVNNGAYSTRAVEVCEYYGLPHIDLKFSVYELPDLSVIEKTLSDNPDI
                     ALVYTTHHETGTGILNPIREIGKIAHSHDAVFVVDTTSSLGMIPFDIGKDNVDFCMASA
                     QKGLQAMTGLSFIVGDESLIRASRDYPKRSYYCNLYLQYDYFEKTGEMHFTPPVQTVYA
                     ARQALDEYFAVGEEAKFARHKRVFEAIHKGLQELGLQYAIK"
     CDS             9285..10778
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg1_1756"
                     /sec_met_domain="Polysacc_synt (E-value: 1.8e-08, bitscore:
                     32.2, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDKKNVVKWNFVFYLLEFLYTILVGIILVPLYLKYIPKDLYGFW
                     LATGNILSILTLINPGFSDVIQQKVAYCYGLHDYKKIGLYTVSGICLTFVFSLIVLIIG
                     VVVNLLIGSIFTMQANYLYELKIAFGLMLVGTFLTINYYSFSSILYAFLSSKSIGLIVL
                     LGNSLGTLISIYLIIHGYGLIALGIASVVRGGVFLSCSVGYVIFKFRKKKIKIVFDLNN
                     VKEIFSFSIYNFLGKTGQTLLTNMNSFLTAKFVSPIDAANLRFTQTVPEFGKVLVLRFL
                     YSVAPIIPNLLAAKGLESIRIKLLNIVYFLISFIFLVCIGFYLLNEQFIYLWLGENNFS
                     GKIINFFIIFLLFFSSINKVCFQFLFALGDIKKSNKILFYQSLLYLPIAVFTTKFCGII
                     GLLLSGILVELLALFIFFFPKMVDILNISKIDIRKKLFVELTKISFVTVITLLIGSCVK
                     FSNPSWLLFFVEIVFIVLTFFVLLFLCSKTFRNLLSICK"
     CDS             10782..11927
                     /locus_tag="ctg1_1757"
                     /transl_table=11
                     /translation="MKILLVFIDMIRVDHLSLYNDNMEKTSLDLFFENLGGTILTNCYT
                     PAPDTPRSIACLQSGLLPYLNGCDSRVKWPRYFMKPNIETIFDIAVGLGYKVNLCATKS
                     DIETGLFKFKENENISIFTTIEDFTDTVHLSENTLSLIAIDDYHSAIDDYGGTSLGIKE
                     GQKVILYSLNNFLTPDIVNKYDYSFFFSDHGHALQSEIVMQKNKLELLSDGRTKLFMFY
                     HNINNKKIVKDSRLASILDLYASIVDLIGSGDFRQGFSFFRDSQRDILHIEDHADFRVS
                     PEQIVSQWRVISDEIDIRTNVFETLNKKGQIIDINVVKKYLDSFSPSFSYYMKQIEILK
                     MYEKMKQRKTHYFNGVKRANWWIRCYVRIINRLYILINRLK"
     CDS             11924..12793
                     /locus_tag="ctg1_1758"
                     /transl_table=11
                     /translation="MIVSSLRGGLGNQMFIYAMVKAMALRNNVPFAFNLTTDFANDEVY
                     KRKLLLSYFALDLPENKKLTFDFSYGNYYRRLSRNLGCHILHPSYRYICEERPPHFESR
                     LISSKITNAFLEGYWQSEKYFLDYKQEIKEDFVIQKKLEYTSYLELEEIKLLDKNAIMI
                     GVRRYQESDVAPGGVLEDDYYKCAMDIMASKVTSPVFFCFSQDLEWVEKHLAGKYPVRL
                     ISKKEDDSGTIDDMFLMMHFRNYIISNSSFYWWGAWLSKYDDKLVIAPGNFINKDSVPE
                     SWFKLNVR"
     CDS             12801..13853
                     /NRPS_PKS="Domain: NAD_binding_4 (4-199). E-value: 6.3e-12.
                     Score: 37.0. Matches aSDomain:
                     nrpspksdomains_ctg1_1759_NAD_binding_4.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     300.8; E-value: 2.1e-91)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg1_1759"
                     /sec_met_domain="RmlD_sub_bind (E-value: 4.7e-15, bitscore:
                     53.4, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 5.7e-12,
                     bitscore: 43.3, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVLVTGAAGFIGSHVCKRLLQRGDEVVGLDNINSYYDINLKYGR
                     LSSLGVPQSELSWYKFTRSNVYPRFSFVRMNLEDRQAMQMLFANGNFDVVINLAAQAGV
                     RYSIENPYAYVESNIDGFLNVLEGCRHSQVKHLVYASSSSVYGLNGQVPFSEKDGIAHP
                     VSLYAATKKSNELMAHTYSHLYNIPSTGLRFFTVYGPWGRPDMSPFLFADAILHGRPIK
                     VFNNGNMLRDFTYIDDIVEGVLRVADSIPEGNQCWDAEVADPSMSCAPYKIYNIGNSRP
                     VKLMDFIRAIEMSIGREADKIYLPMQPGDVYQTYADTSSLSREIGFQPNTSLEAGVKKT
                     ISWYKEFYNL"
     CDS_motif       12810..12872
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg1_1759_0001"
                     /evalue="2.20E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="ctg1_1759"
                     /protein_end="24"
                     /protein_start="3"
                     /score="13.9"
                     /tool="antismash"
                     /translation="LVTGAAGFIGSHVCKRLLQRG"
     aSDomain        12813..13397
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg1_1759_NAD_binding_4.1"
                     /evalue="6.30E-12"
                     /label="ctg1_1759_NAD_binding_4.1"
                     /locus_tag="ctg1_1759"
                     /protein_end="199"
                     /protein_start="4"
                     /score="37.0"
                     /tool="antismash"
                     /translation="VTGAAGFIGSHVCKRLLQRGDEVVGLDNINSYYDINLKYGRLSSL
                     GVPQSELSWYKFTRSNVYPRFSFVRMNLEDRQAMQMLFANGNFDVVINLAAQAGVRYSI
                     ENPYAYVESNIDGFLNVLEGCRHSQVKHLVYASSSSVYGLNGQVPFSEKDGIAHPVSLY
                     AATKKSNELMAHTYSHLYNIPSTGLRFFTVYG"
ORIGIN
        1 gcaggaagaa agaccgcatt gtgtatttgc aatgtgcacc tctgctcaat gatgattttc
       61 aagtggagca aatgtacaac acgctggaga tgatacaaga caattatgaa aagtgggtcg
      121 tttctatgga cgataccaca ttgccctcca aagaagggat tcgtcacata caagtctggc
      181 aactgcctga aatactatag tctgacaatc ttttttgatt gctttgatgt ccggatgatg
      241 ccgtttacga agttaacatc ttgttgttta cgaagttaat atcataattt tacgaagtta
      301 aaatcgtatc ttcgggaagt taacttcctg aagtcacgat tttaacttcg tattagtgct
      361 ttttgaatcg cttttcaatc ccttgagaat cagtgtccgt ttccggagga aaacagctgc
      421 gttctttaat tgattgcaag caaacagcat tttccagaat ataaaatgta ctgtttgtca
      481 gaaaagttct tttttaggtt ccgcttcttt tctttgcccg gttcaacgct gcgatcatgt
      541 attttccttt tgctccgatg ttactccagt tcgtgctgca aatcagtttc catacagggg
      601 ttccttttct cccgtaatcc gacagtcgca ggattgcttc gatctctttt tcgtctgtta
      661 ttccgaatcg ttccaggttt cccattaatc ccacgtagtt gtgtgtcatt gtattaaatg
      721 cgtatccggg catactgacc ggcgtagcat tgacttcctc ttccttcccc cttgggggag
      781 ctgagggggg aaattctcta ctttgcttta ctttactttg ttcggtattg catgcatttt
      841 ttgcatcaga gcctgcatcc tgctctacat tttgcgtgca gacaatcttt tcttcattac
      901 atgtctcttt cggttttatc agtagataag gcatggctgt ccagttcctc tttcttcgct
      961 tggttgcctc tatccatact ttttgtattg cttcagaggt cagtacttta tgttccgcat
     1021 agcttcccct gtctatcatt cctttctcga tcattatctc gataatcccc tgcacctctt
     1081 ctgcgtctgc ctcttttccc gtcttgttgg cgaaaataag gcattgttcc tcaccccacg
     1141 gaatataata tccttcttta tatattttgc atagaagttt cagtacaatc gcgagtcctt
     1201 tcatgccata ttttgcttct attacctcca ttgcgttctc ttccataaaa ttcacagcta
     1261 ttggaaagta atttattcca tcgtatgttg ttgccataat tgttaagttg ttatgttgct
     1321 gattaattgg tcactgaaag attctcctat acgatcgctc tccattccat gaaattctct
     1381 attttgaagg tcatccacct ttgttgctcc acatcccggt agaccactgt gttttttatt
     1441 tccatcggat catactgctt tccgaaatct ttttcgtatc ccaccaatgt tcctttcacc
     1501 agtttgaatg ttccgtcctg tttcatataa gctacggctg catgtccata ttgcatatat
     1561 tctatgaggg cttccagtcg ttctgccatc cattcggagc actgttctgt gtagtccttt
     1621 tcgagcatga tttgtctttt ccattttcct ttcatcattt cgtttttcga tactgctttc
     1681 ctgttctttt tcataattga tttttttaag tttattaatg agttattgtc gggcttttat
     1741 ttttacccat ggcaaagtac ggtaataagt tttgaataga ctaatcctgt aaaagtactt
     1801 tagaaggggc ttttatagga ttttaacaat cggttattcg ctttcatctc gctttttccg
     1861 gaatgctctg gcatattttt tcagttctga tagaaaagaa cgtttattgg gctctgttgc
     1921 aatgcctttg cgctgatatt caaaggtgag atattctttt attgtttcct gtagttcttc
     1981 aatgggtctg ttttccatgc tgttgattcc gcctttgata aagctttcgg taagtgcctc
     2041 cattagcttt cctttgcctt ttgtgatgcg tagtggttct ctaacctttt gattgaggct
     2101 gaattgcaag tcggctttac ccgcaaagat tacttttcca tttacttcga tggttactaa
     2161 gttatcattc aatgagataa ctactttttt tcttccataa tgttagttat taaatgaatt
     2221 tatgaaagga caaagtttgt ataaaaaaat ataaatataa aagaataccg gagttttttt
     2281 agcttaaagg gagaatcttg aactgtaagg atcttgttat tagttattta tcttaagata
     2341 taggtgttgt ttcaaaaaac gttcgtcttt tgaaacagca tttttgagtt ttttttcgat
     2401 gaaaaaatga atattgtaaa gtagctgaac aaagaacttt ttttgctcta tatatatttg
     2461 agaatcagca acataaaatc tttgataaaa ttttatccac taaatatttt gtacattaaa
     2521 acagactcct tacctttgtt caatcaaacg aacgtttttt gaaacacatt cgttcgttct
     2581 caacttctaa aaatgtttcg taaaatttga cggtttgaaa gagaaaagat gtctcgttca
     2641 aaccgcgaag cgcacccgaa acaaaggaaa atttccccaa gtataaatcg taaatgtctg
     2701 aacaacagaa gtattggttt gccgcccgta cccgagataa acaagagttt gctattcgtg
     2761 actctcttga aaaattgaag actgaacttg atctcaatta ctatcttccc actcagtttg
     2821 tcatccggca gttgaaatat cgtcgaaaac gggtggaagt ccctgttatt aagaatctta
     2881 tcttcatcca agctaccaag caagatgctt gtgatatttc taataaatac aatatccagc
     2941 ttttttatat gaaagacctg cttaccaggg ctatgctgat agttcctgat aaacagatgc
     3001 aggacttcat atttgtcatg gatttagatc cgaatggcgt cagtttcgat aatgaccatt
     3061 tatctgttgg tagcagggtt caggtagtta aaggtgattt ctgtggtgtc gagggcgaac
     3121 ttgccagcga ggccaacaaa acttatgttg ttattcgtat tgccggtgta ttgagcgcca
     3181 gcgtcaaagt tcctaaaagt tatttacgtg tcatttaagt tatgaataca ctcacttctc
     3241 aaattgaaca attacagagt ctcgcgcacg agttactcta tttaggtgtg gatggtgctc
     3301 ctatttatac cgatcatttc cgtcagttga acaaagaagt tttagaacaa tccgatgcgt
     3361 tgtatcctca gcgcggtgct actcccgaag aggaggcaaa catttgtctg gcattattga
     3421 tgggttacaa tgcaaccatc tataatcagg gtgacaagga ggagaaaaag caagttgttc
     3481 tcaatcgttg ttgggacgtg cttgaccaac ttcctgctac tttattgaaa tgccagttgt
     3541 tgacttattg ttatggtgag gtgtttgaag aggagttggc gaaagaggcg catactatca
     3601 tagaaagttg gagcaatcga gaacttttaa aagctgaaaa agaaatagct gaaagtttga
     3661 ataatttgga agctaatcca tatccgtaca gcgaactaca cgaatgattc gttcctttct
     3721 ttcccgttct cccctttacg ttttggctct ctatgccagc gttttgattt ttctggttta
     3781 tacttgtgcc tatgcctacc gtaagccatt taccgcagcc atttatgaag gtgagattct
     3841 ttggggattc gatgtcaaaa tcctgtatgt cctttctgag attatcggct acgccctttc
     3901 caagttcatc ggtgttcgta ttctgccttc gatgaaagcg ggtcaccgca tctattatat
     3961 aatagggctg cttacttttt ccgaagttgc tttgttgggt tttgcacttc ttccggttcc
     4021 tttgaaggtg tgttctattt ttctgagcgg tcttcccctg ggtatgatct ggggtgtcat
     4081 ttttagctat atcgaaggac gtcgtatcag tgaaatattg aatgtcgggc tgagcgttgc
     4141 tttgatagtc tcttccggtt tggtgaaaac cttgggacaa ttcgtgatgg ataatttgca
     4201 tgtgtcggaa tattggatgc cttttaccac cggtttgttg gtataccctg ttatgttgct
     4261 ttgcagctgg cttctcaatc agattccggc tcccaatgag caagatatca tacagcgtac
     4321 caaacgtgct ccgatggatg gtaaagacag gcgtaaattt ttgaggcaat tcttctgggg
     4381 aatctgcatg ctggttgttt tttacggtgc cctgaccgtg ttccgtgagc ttcgtgactc
     4441 tttcgctgcc gatatttgga aggagctcca tattgaaggt gcaatgatct ttactcagac
     4501 agaggttccc attgccgttt ttgtccttgt actgatgttc ctgattgtgt ttgtccgtaa
     4561 taaccgtttg gctcttaata tcatttattg cattgccgtt acaggcggtt ccttaatggt
     4621 tttttccaca ttgctttatg tgtacggttt tctttctcct atttggtgga tgattctttc
     4681 cggtcttggt ctgtatatgg ggtatattcc tttcacttac ctgatagaac gcctgattgc
     4741 ttcccttagg gtggttagta cggctgtttt tattatttat ttggccgatt ctttcggcta
     4801 tctgggcact acgggtgtgt ttatggtcaa gaacttcgtt tctgctgaca tttcctggac
     4861 tgtcatgctg atgcgtactg cccttttttc aggatttgtt tctgtcctgt cgatttttgc
     4921 gatttactgt tatttcaagc aacaattgaa ttcacttata ttaattccct ctgaaattca
     4981 tgattaaaac agcaatgatc atggctgccg gtcttggtac ccgcttcggg cattataccg
     5041 agcttgtgcc caaaggtttt gtggaggttg gtggaaaacc tatgattatc cgctctatcg
     5101 aaacccttct ttcctgcggt atcgaacgta tcatcctcgg tacgggttat aaaaaagagg
     5161 cttatgaagc ccttcaggcg gatttccccc agatagagac ctgtttcagt ccccgctatg
     5221 ccgatactaa cagtatgtac actctgtaca atacgcgtga cgtgataggc gatgacagtt
     5281 tcctgctgct tgaatcggac cttgtttttg aacgcaaggc catcctctcg ctgctggatg
     5341 acgaattccc ggatgtgatg cttgtctcct ctctgaccaa gttccaggat cagtattatg
     5401 tggaatatga ctgcaatcac atccttactt cctgttcggt agacaaggac gcccttgagg
     5461 caaaaggcga gctggtcggc atccacaaac tttccaatac tttctaccgc cggatgtgcg
     5521 ccgattatgc gaccatcctg gagtcccagc ctaaactggg ttacgagtac gaactgctcc
     5581 gcatgtcccg ttccgtttcc cctgtccgtg tgctcagggt ggagggtctt aaatggtatg
     5641 aaatcgatga tgaagccgac ctttcgtatg ccgaggagca catcatccgc tattgttaaa
     5701 aacaaatctt ttccatatat gaacaagaaa atctatgtcg gtatgaccgc ggatatcatg
     5761 catccggggc tgatccatat tattcatgaa gctactaaat acggtgatgt catcgtcggt
     5821 ctgctgacgg acaaggccat cgccgaacac aaacgcctcc cttaccttac ctatgatcag
     5881 cgtaaggagg ttgttgagaa tatcaaaggg gtttgcgaag ttgttcccca ggaggattgg
     5941 agctatgttc cgaatctgga aaaactcaag cctgactata tcatccacgg tgacgactgg
     6001 aaaacgggcc cgttgcgcga ggtgcgcgaa caggtttttg aggtgatgaa cgcccagggg
     6061 ggcaaggtaa tcgagatccc ttatacccgg ggcatcaact cctcctcgct cgacaaggat
     6121 atcaaggcga tcgggactac ccctgacgta cgtcttaagt ccctccgccg cctgatccag
     6181 gccaagcctg tggtccgtat cctggaggct cacgacggtc tttgcggtct gattattgag
     6241 aatcttgaag tccagaaggg tgataagcgc gaggtctttg acggcatgtg gtccagcagc
     6301 ctgaccgact ctacgagtaa gggtaaaccg gatattgaag cggtcgacct taccacccgt
     6361 ttacaggacc tgaacaatat ccttgaatgt accaccaagc ctattatttt tgatggagac
     6421 acgggtggca agatcgaaca ctttgtcttt actgtgcgca ctctggaacg tcacggcatt
     6481 tctgcggtaa tcattgaaga taaagtcggt ctgaagaaga attccctttt tggtactgac
     6541 gctatccaga ctcaggatag tatcgagggt ttctgcgata agatcagagc agggaagaat
     6601 gcccaggtca ccggtgactt tatgatcatt gcgcgtatcg aaagccttat tgccggcaaa
     6661 cccatgagtg acgccctcga acgtgcttat gcctatgtac aggcgggtgc ggacggcatc
     6721 atgatccaca gcaaggataa gtcgggtgag gatatccgtg agttctgccg gactttccgc
     6781 aaagaatacg cccatgttcc cattgtcgtt gttccgacga cttatgacca tgtgcacgaa
     6841 tccgaacttc acgaatgggg tgccaacgta gtcatttacg ccaaccacct tctccgtgcc
     6901 gcttacccgg ccatgatgaa cgtggccagg accatccttg aaaacgagcg cgccgaggag
     6961 gtccgtccgc tttgtatgcc tatcaaggaa attctggaat tgattccggg cactaaataa
     7021 atcagacggt tatggtaagt gtagaaaaga tatacgatac gttccttcat cacggtgttg
     7081 acttctttac gggtgttccc gattcccttt tgaagaatat ttgcgcctat atcacggatc
     7141 atgctccgcg tggtaagcat atcatcgccg ccaatgaagg ttccgcggtg ggcattgcct
     7201 gcggttatta tatggcttct ggcaacgttc ctttggttta tatgcagaac tccggtctgg
     7261 gaaatacggt gaatcccctc ttgtcccttg cggacgagaa agtgtacagc cttcctcttc
     7321 ttttgctggt cggctggcgc ggtgagcccg gcacaaaaga cgaaccccag cataagaagc
     7381 agggcgaggt tacccttgat cttctgaaag ccatgaaaat tccttttata attctggata
     7441 cggatgaaga cgaggccctg gcgcagatcc atgacgcggt tgtctcggcg aagagccggt
     7501 cgattcccca tgcgattgtc atccgtaaag atacgtttgg aaaatataaa ttgcgtcagg
     7561 aagccttgaa tgactatcct ctgtcccgcg aggaggcgct aatgcaggtt gtcgaccacc
     7621 tcggggacaa tgacattgtg gtttccacca cgggcaagct ctcccgtgag cttttcgagt
     7681 accgtgaatc gaaagggcag gggcacgcaa gggatttcct aactgtgggt tccatgggtc
     7741 acagttcttc cattgccctg gggattgccc ttgagaagcc tgaccgccgt gtctactgtc
     7801 ttgacggtga cggtgccttt atcatgcaca tgggtgctgt cagcaatatc ggcgatcttt
     7861 ctcccaagaa ttacaggcac gtccttttca acaatggtgc ccatgaatct gtcggcggtc
     7921 aacccacgct cggtttccgt ctggatatcg cctcgatagc gaaaggttgc ggttatgcgc
     7981 atgtactgac tgcctccgac aaggaagggc tttcgtgtgc gcttgagaag ctgtccggcc
     8041 tgtccggccc ggttcttctt gaaattaagg tccggatcga ttcacgtgat gatctgggtc
     8101 gccctacgac tactccggta gagaataaag agcattttat ggcattttta aaagatacgc
     8161 attcatgaat atcgaaagaa agatcctttt gaatccgggt cccgccacca cgacggatac
     8221 ggtaaagctg gcgcaggttg ttcccgatat ctgtccgcgt gagaaagagt ttgcgggtat
     8281 gatgaaacag ttacgtgacg acctggtccg cgttgcgcat ggagacctct ccaaacatac
     8341 ggccgtcctg ttttgcggct cgggtacgat caatatcgat atctgcctga actctcttct
     8401 tcctgcggat aagaaagttc tggttgtcaa taacggtgcc tacagcaccc gtgcggtgga
     8461 agtctgcgaa tattacggtc ttccccatat cgatttgaag ttttccgttt acgaacttcc
     8521 cgacctgtcg gtaatcgaaa agacgctttc cgacaatccg gacatcgctt tggtctatac
     8581 cacgcaccat gaaacgggta cgggtatctt gaaccccatc cgtgagatcg gaaaaatagc
     8641 ccattcgcac gatgccgttt ttgttgtcga caccacttct tccctgggta tgattccttt
     8701 tgatatcggg aaggataatg ttgacttttg tatggcttcg gctcagaaag gccttcaagc
     8761 catgacgggt ctttccttta tcgtaggtga tgaatccctg atacgtgctt caagggatta
     8821 tccgaaacgt tcctactatt gtaacctcta tttacaatac gattactttg agaagacggg
     8881 tgagatgcat tttactcctc ctgttcaaac cgtttacgct gcccgtcagg ctcttgatga
     8941 atattttgca gtaggtgaag aggctaaatt cgcccgtcac aagagggtct ttgaagccat
     9001 ccataaaggt ttgcaagagc ttggtcttca gtatgcgatc aaacgtgaat ggcagtcggg
     9061 tcttgttgtt tctgtattgt atcctgatga tcccctctgg gattttgata aggttcatga
     9121 ttactgctat gaacgtggct ttacgatcta cccgggtaag atttccacaa cgaatacatt
     9181 ccggctttgt gcgttgggag agattgatgt ggaggatatt gagaggttct ttgaggtgat
     9241 gagggaggct gttttaaaat actagagaaa ttgtcaattg ggatatgaaa gataaaaaaa
     9301 atgtagtaaa atggaatttt gtattttatc tcttggaatt tttgtataca attttagttg
     9361 ggattatttt ggttccgttg tatttgaaat atattccaaa ggatttatat ggtttctggc
     9421 tagctactgg taatatattg tctattttaa ctcttatcaa tcctgggttt tcagatgtga
     9481 tacaacaaaa agttgcatat tgttatggat tacatgatta taaaaaaata ggattatata
     9541 ccgtttcagg tatttgtctt acgtttgttt tttcattaat tgttttgata atcggagtag
     9601 tagttaacct tttaataggt agtatattta caatgcaagc taattattta tatgaattaa
     9661 aaatagcttt tggcttaatg cttgttggta catttttaac tattaattat tattcctttt
     9721 cttctatatt atatgctttt cttagtagta agtcaatcgg gctaattgta cttctaggca
     9781 atagtttagg tactttaata tctatttatc ttattatcca tggatatggt ctaatagctt
     9841 tgggaattgc atcagttgtt cgaggaggtg tttttttgtc atgtagtgtt ggctatgtaa
     9901 tatttaaatt tcgaaaaaag aaaataaaaa ttgtatttga tttaaataat gttaaggaga
     9961 tcttttcttt ttctatatat aattttttag gtaaaacagg acaaacattg ttgactaata
    10021 tgaattcttt cttgactgct aagtttgttt ctcctattga cgctgcaaat ttgagattta
    10081 ctcaaactgt gccggaattt ggtaaagttt tagttttaag atttttatat tctgttgctc
    10141 ctattattcc taatttgtta gcagctaagg ggttggagtc tattagaata aagcttttga
    10201 atattgtgta ttttttgatt tcctttattt tcttagtatg tattggtttt tatctattga
    10261 atgaacaatt tatatattta tggcttggtg agaacaattt ttcagggaaa ataattaatt
    10321 tttttatcat ttttttattg tttttttctt ctataaacaa ggtctgtttt cagtttcttt
    10381 ttgcattagg agatataaag aaaagcaata agattttatt ttaccaatct ttgttgtatt
    10441 tacctattgc tgtttttaca acgaaatttt gtggaataat tggtttattg ttatctggaa
    10501 tattagtaga attattggca ctattcattt tcttctttcc taaaatggtt gatattctta
    10561 atatttcgaa aatagatatt agaaagaaac tttttgtgga gttaaccaaa atatcttttg
    10621 ttacggttat tacattatta ataggttctt gtgtcaagtt ctctaatcct agttggctac
    10681 ttttctttgt ggaaattgtt tttattgtat taacattttt tgttttgttg tttttatgtt
    10741 caaagacttt tcgtaattta ttgtctattt gtaaataaaa gatgaagata ctactagttt
    10801 ttatagatat gatacgtgtt gatcatttat cattatataa tgataacatg gagaaaacat
    10861 cgttggattt gttttttgaa aatttaggag gtacaattct aactaactgt tatacacctg
    10921 cacctgatac tccaagaagt atagcttgtt tgcaaagtgg cctattaccc tatttgaatg
    10981 gctgcgatag tcgtgtcaaa tggccaagat attttatgaa acctaatata gaaactattt
    11041 ttgatattgc agtagggtta ggatataaag taaatttgtg tgcgactaaa tctgatattg
    11101 aaacagggtt gtttaagttt aaagaaaatg aaaatattag tatatttact actattgaag
    11161 attttactga tactgttcat ttgtcagaaa atacattgtc cttaattgcc attgatgatt
    11221 atcattctgc cattgatgat tatggaggaa cctcattggg aattaaagaa gggcagaaag
    11281 tgatattata ttctttaaat aattttctaa ctccagatat cgtaaataaa tacgactatt
    11341 cgtttttctt ttcagaccat gggcatgctt tgcagagtga aattgtcatg caaaaaaata
    11401 aactagaact acttagtgat ggaagaacta aactttttat gttctatcat aatattaata
    11461 ataagaaaat agtaaaagat tctaggcttg cttctatttt ggatctttat gcctctattg
    11521 ttgatttgat tggtagtgga gattttaggc aaggtttttc cttttttaga gacagtcaaa
    11581 gagatatact tcatatagag gaccatgctg attttagggt gtctccagag caaattgtga
    11641 gtcaatggcg agtaatttca gatgaaatag atattaggac taatgtattt gaaactttga
    11701 ataaaaaagg acaaataatc gatataaatg tggtaaaaaa atatttagat agtttttccc
    11761 cctcgttttc ttattatatg aaacagatag agattctaaa aatgtatgag aaaatgaaac
    11821 aacggaaaac gcattatttt aatggggtaa agcgagctaa ttggtggatc cgctgttatg
    11881 ttagaattat aaataggtta tatatactaa taaatagatt gaaatgattg tatcatcttt
    11941 gcgaggagga ttggggaatc aaatgtttat ttacgctatg gtgaaggcca tggcattaag
    12001 aaacaatgta ccattcgctt ttaatttgac tactgatttt gcaaatgatg aagtttataa
    12061 aaggaaactt ttattatcat attttgcatt agacttgcct gaaaataaaa aattaacatt
    12121 tgatttttca tatgggaatt attatagaag gctaagtcgt aatttaggtt gtcatatact
    12181 tcatccatca tatcgttata tttgcgaaga gcgccctccc cactttgaat caaggttaat
    12241 tagttctaag attacaaatg cttttctgga aggatattgg cagtcagaaa aatattttct
    12301 tgattataaa caagagataa aagaggactt tgtaatacaa aaaaaattag aatacacatc
    12361 gtatttggaa ttggaagaaa taaaattgct agataagaat gccataatga ttggggttag
    12421 acggtatcag gaaagtgatg tagctcctgg tggagtgtta gaagatgatt actataaatg
    12481 tgctatggat attatggcat caaaagttac ttctcctgtt ttcttttgtt tttcacaaga
    12541 tttagaatgg gttgaaaaac atctagcggg aaaatatcct gttcgtttga taagtaaaaa
    12601 ggaggatgat agtggtacta tagatgatat gtttctaatg atgcattttc gtaattatat
    12661 aatatcgaat agctcttttt actggtgggg agcatggctt tcgaaatatg atgataagct
    12721 ggtgattgct ccaggtaatt ttataaataa ggattctgta ccagaatctt ggtttaaatt
    12781 gaatgtaaga taaaatctta atgaatgtat tagtaaccgg tgctgccgga tttataggtt
    12841 cgcatgtttg taagcgtctt ttgcaacgtg gggatgaagt tgtgggcttg gataatatta
    12901 attcgtatta tgatattaat ttaaagtatg gacgcctttc gagcttaggt gttcctcaat
    12961 ctgaactgtc atggtataag ttcacacgga gtaatgttta tcctcgattt agttttgtgc
    13021 ggatgaacct cgaggatagg caggctatgc aaatgctgtt tgctaatgga aattttgatg
    13081 tagtaatcaa tttggccgca caagcgggag tgcgctactc cattgagaat ccatatgctt
    13141 atgttgaaag taatatagac ggttttctga atgttctcga gggttgtcgt cacagtcagg
    13201 tgaaacattt ggtttatgcc agttccagta gtgtatatgg tttgaatgga caggttcctt
    13261 tttcagagaa agatggcata gcccatccgg tgagtctgta tgccgcaacc aagaagtcga
    13321 atgaacttat ggcacatact tatagccatt tatataatat accttctacg ggtcttcgtt
    13381 tcttcacggt atatggtccc tggggtagac cggatatgtc tccttttcta tttgcggatg
    13441 ctatcttgca tggtcgcccc atcaaggtct ttaacaatgg caacatgctt cgtgatttta
    13501 catatataga tgacattgtg gaaggtgtct tgagagtggc tgattctatt ccggaaggga
    13561 atcagtgctg ggatgctgag gttgcggatc caagcatgtc ctgtgctccc tataagattt
    13621 ataatattgg taattcccgt cctgtaaaat tgatggattt tatacgtgct atagaaatgt
    13681 caatcgggag ggaagctgac aagatctatc ttccgatgca gcccggggat gtgtatcaga
    13741 cctatgcgga tacttcttct ctttcgcggg aaattggttt tcaacccaat acgtccttgg
    13801 aggcgggcgt taagaaaaca ataagttggt ataaagaatt ttataatcta taattatgaa
    13861 gattgctata gttggtacag gttacgtcgg tttggttaca ggtacctgtt tctctgagat
    13921 gggagtagac gtcacatgcg ttgatgtgat tgaatctaaa attgataatc ttaaaaaagg
    13981 cataattccg atctatgagc cgggacttga agacatggtg caccgcaatt acaatgcggg
    14041 gcgtttgaag ttcactactt ccttagcctc atgtttgaat gatgttgagg ttgtgtttag
    14101 tgcagttggt actcctcctg atgaagatgg cagtgcggat ttaaagtatg tgcttgaagt
    14161 tgcccgtacg attggtaaaa cgatgaacca ttatgtactg gtggtaacga agagtactgt
    14221 tcctgtcggc acagcgcaac aggtgaaagc tacgatccgg ggtga
//
