LOCUS       c00014_gnlPro..         7804 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EPMDDCDP_16.
ACCESSION   c00014_gnlPro..
VERSION     c00014_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-03 16:36:43
            Original ID  :: gnl|Prokka|EPMDDCDP_16
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7804
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7804
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1093:4085]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1094..4085
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7804
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7804
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             1094..3346
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg14_1"
                     /sec_met_domain="Gly_radical (E-value: 1.8e-42, bitscore:
                     132.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.7e-268, bitscore:
                     877.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQKEQWQGFNGRIWREEINLREFIQDNYTPYDGDASFLEGPTEA
                     TDTLWGELQKLQKAEREKGGVLDMDTDIVSSLTSHKPGYISESLKEKEQIVGLQTDKPL
                     KRAFMPFGGIRTAEEACTTYGYTPNPEFHKIFTDYHKTHNQAVFDSYTPEMKKARHNKI
                     ITGLPDTYGRGRIVGDYRRVALYGIDLLVADKQKDFANCGDGTMTDDVIRLREEISMQI
                     KALKDMKVMAESYGYDISEPASNAKEAVQWTYFGYLAAIKTQNGAAMSIGRIATFLDIY
                     IKRDMDKGILTEKEAQELIDHMTMKFRMVKFGRIPAYNQLFSGDPVWATLEVGGLGQDG
                     RSMVTKTDYRFLHTLENMGPSPEPNLTVLYSERLPKAFRDYAAHISITTSSIQYENDDV
                     MRPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKTGQQVGPEYKP
                     ITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYEYALMALIDTNVRRTFA
                     TGIAGFSHVVDSLSAIKYAKVKTVRNEEGLVVDYETTGDFPKYGNDDDRADDIAVWLLQ
                     TFMKKLEKFHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQ
                     NGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERIGNLVNVMDGYFAQGAHHL
                     NVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYAVKFISLTKEQQLDVIARTCHDRM"
     CDS             3333..4085
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.5;
                     E-value: 3.4e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg14_2"
                     /sec_met_domain="Radical_SAM (E-value: 4.9e-32, bitscore:
                     99.3, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTECNKNSMALVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNP
                     DTWKMTEENGAIWKNAEELLNQALRYRPYWKNGGGITVSGGEPLLQIDFMLEFFKKAKE
                     KGIHTVIDTAGGPFTRKEPFFSKFQELMKYTDLLLVDIKHIDTECHKVLTGHSNENILD
                     MIRYLSDIKKPIWIRHVLVPERSDKDEYLTRLADFIHSLDNVEKVEILPYHTMGIYKWK
                     ELGLEYPLEGIQPPTKERVKNAKKILAI"
     CDS             complement(4276..7467)
                     /locus_tag="ctg14_3"
                     /transl_table=11
                     /translation="MKNNHNLLMKMKNLEKFSGAIKRCVAIAMATMLFATTGFSLDTHP
                     VKAQEVSSSADKIEIPEISKDAFKKYKKISGIGANTILGADFTYYQQCLEWGKSYKNYM
                     SQSVDNIFDYVKSQGINTISLKVAVNPTGENAYLSLDNAIKTLKAVKASKANLKTNLVL
                     LYSDEMTYAGENGQKLPADWEKAEKAEQSVSRVESAKTYTKEIIAKLKQVNVLPDIVTI
                     GNEVDWNFLGITDGEGWEGWKAMGDISALLKKEGVKNAVSIAAPSDAASVKYIVQKLGY
                     ASVDYDYIGVNVYPDNNTNNYIKSLKKEVESCASDKQLIVSNVEYERVNEANTANVYTQ
                     ADSIYNLLEATIDEKNAGGLIYNEAAYAGSWKSFFDDEGDAQVSMAIFAYAQGHETDTS
                     RDPYKYGDDTGLKQQKVTIKKVQNMSDSTIRGIDISSYTALKKAGVKYYDNEGKEASLL
                     KVLSDNGVNYIRIRIWNDPYNEKGETYGGGSNDVKAGLEIAKEAAKYNIKVLLGFHYSD
                     FWADPAVQLLPKAWKKDRNNQEKMCSNVYEFTKETLEQFKDAGADIGMVQVGNEISQGM
                     MGIMHKTKANVWQEEEKSTIIDSYLSAGARAVRECTPDALVAIHLDNLNLGMYKDAMNA
                     WERDNVDYDVLGASSYAFWAGKNMLKNVRKAGEYVASRGKLFAVLETSWLNSQKDADGT
                     VNMVNNTNGAVYKVGPQGQADMLSDLYNAILSNDNGLGAFYWEGAWIPVRAGWVNWKYN
                     KEMANEFGTGWAAENAEGYYPTSKLYYNGNPVWGGNSWDNQTLFDDKGYPLDSLRFYKD
                     AVSSNEKYSRVVIALCDKENNVLEYRVVKVVSGKSMTYILPDIKGYTKEKDTIKILGTN
                     DKISKVSVVYNKDIKKQAITVKKASYTIPYGTKFNLKNEVKAVGSLTFTSNNTNVVSVE
                     KQGKKLVVKKPGKVTIKITAGATADYKQASRIVTIYAVPKKQTIKKVSTSKRKVKVNIK
                     KDAKATGYQIVAAKNSKFSKGKKVLTKKGIRQVTYTITKLDSKKIYYVKARSYKKIGNK
                     KYFGPWSKVKKIRVK"
ORIGIN
        1 ggggagaggg tagaagtttt ctgcttgctc ttaaaatatt gtagatagat attttttatc
       61 ccagtttgat tcgtttatct tttattgtaa gcttctaaaa gattattcct aaactattat
      121 tactgattgg atagtagact agatttcaaa caaatttggt gcaattatta tgttatcgtc
      181 cgcaatattt ataaaaccaa atttaaaatg ccgatttctt cattttacta ttacatatag
      241 tcaagtcaaa tcatccccct attcccagtc attctcgagt aagcagaaga catcagcttc
      301 tgtccccaaa tcccctcctc ctatttaatt caccttctcc agccatagca cagaagccat
      361 tgccccgaat aaggcatatt tgtggtctcg gagcaagtgt tggatgcgag cgagtagagc
      421 gaacattcaa cacgccgcga ctaaaatcac aaatatgcct tattcggggc aatggcttcg
      481 tctgtagttc gcttaatgaa ttaaatagta accccttgac attttgttct attttgttta
      541 tagttaggat acttatacga ctgacggaag taagtggaat gaaccacatg aagtataaga
      601 ctcttatagc cgaccgtctg ggcaattctt gtctggatgc tggctttttt atttctgata
      661 agataatatt ctatccaata tccctgataa gaagtaactt taaatcgtta ttaagtcatg
      721 tgctaaagaa gatgtttttc caaacatgct ctaaagagcg aaaaataaaa ttatagttgt
      781 atgcttgtca ttattgtact tatatcagta catttgtacg atttgataat attttatcaa
      841 cgctctaaaa ccctttattt taaaggctct acaagcattt ttgtgtatat gttcaaaaaa
      901 aagaacacat aatatcaaaa aattgtttga cgattgccaa aaatcatgtt aataatgttg
      961 ttagatgatt aaaacttaaa taagttaaaa ctaatcatag taaatcatct gaacaatttt
     1021 gttgattttt tagcaaacat atttcaaaac cttgacttaa ggattttttt aacaaacagg
     1081 gaggtcaatt aagatgttac aaaaagaaca atggcaggga tttaatggac gaatctggag
     1141 agaagaaatt aatttaagag agttcatcca ggataactac actccatatg atggagatgc
     1201 ttctttcctc gaaggtccta ccgaagctac tgacacactg tggggcgaac tgcagaagct
     1261 gcagaaagca gagcgtgaaa agggtggtgt tcttgatatg gatactgata tcgtatcaag
     1321 ccttacttcc cataaaccgg gttacatttc tgagtcttta aaagagaaag aacagatcgt
     1381 aggtcttcag acagataaac ctttaaaacg tgcatttatg ccatttggtg gtatccgtac
     1441 tgctgaagaa gcttgtacaa cttatggtta cacaccaaat ccagaattcc acaagatttt
     1501 tacagattat cataagacac ataaccaggc agtatttgat tcttatactc ctgaaatgaa
     1561 gaaagctcgt cacaataaga tcattactgg tcttccagat acttacggac gtggacgtat
     1621 cgttggtgac taccgtcgtg tagctcttta cggtattgac ctgttagttg ctgacaagca
     1681 aaaagatttc gcaaactgcg gtgacggaac aatgacagat gacgttatcc gtttaagaga
     1741 agaaatctcc atgcagatta aagcattaaa agatatgaaa gttatggctg aaagctatgg
     1801 ctatgatatt tccgaacctg cttctaacgc aaaggaagct gttcaatgga cgtatttcgg
     1861 ataccttgca gcaatcaaga cacagaacgg tgctgctatg agtatcggac gtatcgctac
     1921 tttccttgac atttatatca aacgtgatat ggataaggga atccttacag agaaagaagc
     1981 gcaggaatta atcgaccata tgactatgaa gttccgtatg gttaagttcg gacgtatccc
     2041 agcttacaac cagctcttct ccggtgaccc tgtatgggca acacttgaag ttggcggact
     2101 tggacaggac ggtcgttcca tggttacaaa aactgactac cgtttcctcc acacattaga
     2161 aaatatggga ccatccccag agccaaacct cacagtactt tactctgagc gtcttccaaa
     2221 agcatttaga gattacgcag ctcatatttc tattacaaca agttcaatcc agtatgaaaa
     2281 tgatgatgta atgcgtccag tatggggcga tgattactcc atctgctgct gtgtttctgc
     2341 tacacagact ggtaaggaaa tgcagttctt cggagcccgt gcaaaccttg caaaatgtct
     2401 cctctacgca atcaatggtg gtgtagatga aaagactggt cagcaagttg gtccggaata
     2461 caaaccaatt acttccgaat atcttgacta tgatgaagta atgcacaaat atgatatcat
     2521 gatggactgg ttagcaggtt tatatgtaaa tacattaaat ctcatccagt acatgcatga
     2581 caaatattac tatgaatatg ctttgatggc cttaatcgat acaaatgtac gtcgtacatt
     2641 cgctacaggt atcgcaggat tctcacatgt agtagattcc ttaagtgcga ttaaatatgc
     2701 caaagtaaaa acagtccgca atgaagaagg acttgttgtt gattatgaga caactggcga
     2761 cttccctaaa tacggaaatg atgatgaccg cgctgatgat atcgctgtat ggttacttca
     2821 gacatttatg aagaagcttg aaaaattcca tacttaccgt gattctgaac ctacaacttc
     2881 catccttact atcacatcaa acgtagtata tggtaaggca actggttccc ttccagacgg
     2941 aagaaaggcc ggagaacctc tttccccagg agcaaaccca agttatggcg cagagcagaa
     3001 cggtctctta gcttccttaa actctgtagc taaacttcct tatgaatggg cattagatgg
     3061 tatttccaat acgcagacaa tcaaccctga tgcactcggt cattccgaag aagaacgtat
     3121 tggtaactta gtaaatgtta tggacggcta cttcgcacag ggtgctcacc acttaaacgt
     3181 aaatgtattt ggtgttgaga aactgattga tgcgatggaa catccggaaa aagaagaata
     3241 cgcaaacttt acgatccgtg tttcaggtta tgcagttaaa ttcatctctc tgacaaaaga
     3301 acagcagtta gatgtaattg ccagaacatg ccatgacaga atgtaataaa aattcgatgg
     3361 ctcttgttca ttctacggag agttttggtt cggtggatgg gccgggggtt cgttttattg
     3421 tttttttgca gggctgccct ttgcggtgtc agttctgtca caatccagat acatggaaga
     3481 tgacagagga aaatggtgcg atatggaaaa acgcagagga gctgttaaat caggcactcc
     3541 gctatcgtcc ttactggaaa aatggcggag gtattactgt cagtggtgga gagcctcttt
     3601 tacagattga ttttatgctt gagttcttta aaaaggcaaa agaaaaagga atccacaccg
     3661 ttattgatac agctggtgga ccttttacaa gaaaagaacc tttcttttct aaatttcagg
     3721 aattgatgaa atacactgac ttgttattag tagatatcaa acacatcgat actgaatgcc
     3781 acaaagtttt aactggccac agtaacgaaa atattcttga tatgattcgc tatctttccg
     3841 atatcaaaaa accgatttgg attcgccatg ttcttgtacc ggaacgaagc gataaagatg
     3901 aatatctgac tagattagct gatttcattc actctcttga taatgtggaa aaggtggaaa
     3961 tcctgcctta tcatacgatg ggtatttata aatggaaaga acttggatta gaatatcctt
     4021 tagaaggaat tcaaccacca acaaaagaac gggtgaagaa cgctaagaaa attcttgcta
     4081 tttgatttgc cgacaaacaa tgtactaaaa gaaaaatccc ccatatttgt gacttcgaag
     4141 atagattgcg ttgaatactt atccatcttt aatctatcct tgaaatcata aatatggaga
     4201 attttgtctt taaatataaa ctttagttaa actccactca aattaaattc tctttctcca
     4261 aataatctct aaaatttact ttactcttat tttctttact ttgctccatg gaccaaaata
     4321 tttcttattg cctatttttt tataagaacg tgcttttaca tagtaaattt tcttagaatc
     4381 taattttgtt attgtatatg ttacctgtct tattcctttc tttgttaata ccttcttgcc
     4441 cttgctaaat tttgagttct tagctgcaac aatctgatat cctgtagctt tggcatcttt
     4501 ctttatattc acttttactt ttctcttaga tgtagaaacc tttttgatgg tctgcttttt
     4561 cggaactgca taaatagtaa ctattctact tgcctgttta taatctgctg ttgcaccggc
     4621 tgttatctta atcgttactt ttcctggctt ctttacaaca agttttttgc cctgcttttc
     4681 aacagaaaca acattcgtat tatttgaagt aaatgttaaa cttccaactg cttttacttc
     4741 gttcttaaga ttaaacttcg ttccataagg aatcgtatag gatgcttttt tcacagtaat
     4801 tgcctgtttt tttatatcct tattataaac tacagatacc ttagatatct tatcatttgt
     4861 tcctaaaatt ttaatcgtat ctttttcttt cgtatatcct ttaatatccg gtaatatata
     4921 agtcatactc tttcctgaaa caacttttac taccctgtat tcaagtacat tattttcctt
     4981 atcacataat gcaataacca ctctggaata tttttcgttg gatgacactg catctttata
     5041 gaagcgaaga gaatctaatg ggtatccttt atcgtcaaaa agcgtttgat tatcccatga
     5101 atttcctccc caaacaggat ttccattata atacaactta ctcgttggat aatatccttc
     5161 tgcattttct gccgcccatc ctgttccgaa ttcatttgcc atttctttat tatatttcca
     5221 atttacccaa ccagctctta caggaatcca tgcgccttcc caataaaaag cacctagtcc
     5281 attatcattt gataaaattg cattatataa atccgacaac atatctgcct gtccctgagg
     5341 accaacttta taaactgcac cattagtatt attaaccata tttacagtac catctgcatc
     5401 tttctgagag ttaagccatg atgtttccaa tactgcaaat agtttgcctc ttgatgcaac
     5461 atattctcca gcttttcgaa catttttaag catatttttt ccagcccaaa aagcataact
     5521 agacgctcct aaaacatcat aatctacgtt atctctttcc catgcattca tggcatcttt
     5581 atacattcct aagttaaggt tatctaaatg tatcgcaacc aacgcatctg gagtacattc
     5641 tcttactgct cttgcccctg cgctcaaata actatcaata attgtagatt tttcttcttc
     5701 ctgccataca ttagccttgg ttttatgcat aattcccatc ataccctgag atatctcatt
     5761 tccaacttgt accataccaa tgtcagcacc agcatcttta aattgttcta aggtttcttt
     5821 agtaaattca tatacattag aacacatttt ttcctggtta tttctgtctt ttttccatgc
     5881 ttttggaagc aactgtactg ctggatctgc ccaaaaatca gaataatgga atcccaataa
     5941 aactttaata ttatatttgg ctgcctcttt cgctatttct aacccagcct taacatcatt
     6001 ggatccacca ccataagtct ctcctttttc attatacgga tcattccata tacgaatacg
     6061 aatgtaattt acaccattat cacttaaaac ttttagtaat gaggcttctt ttccttcatt
     6121 atcataatat tttaccccag ccttttttaa ggcagtatac gatgaaatat ctatgcctcg
     6181 aatagtagaa tctgacatat tctgtacttt tttaatggtt actttctgct gttttaaacc
     6241 agtatcatct ccgtatttat aaggatctct gcttgtatct gtctcatgac cctgtgcata
     6301 tgcaaaaata gccatggaaa cctgtgcatc tccttcatca tcaaagaaag acttccagct
     6361 tccagcatat gcagcttcat tataaatcaa accaccagca ttcttctcat caattgtggc
     6421 ttctaacaag ttataaatgc tgtcagcctg tgtatataca ttggctgtat tcgcctcatt
     6481 tacacgttca tattctacgt tagaaactat cagttgttta tccgatgcac aactttctac
     6541 ttcttttttt aaacttttaa tataattgtt tgtattatta tctggataaa catttactcc
     6601 aatataatca taatctacgg acgcatatcc caatttctga acaatatatt ttacagaagc
     6661 ggcatcagat ggcgcagcta tgctgacagc attctttaca ccttcttttt tcagcaatgc
     6721 tgaaatatcg cccattgctt tccagccttc ccagccttct ccgtcagtaa ttccaaggaa
     6781 attccaatcc acttcattac caatcgttac aatatcagga aggacattca cctgctttaa
     6841 ctttgcaatt atctcttttg tatatgtttt tgcactttcc actcgactta ccgattgctc
     6901 tgctttctct gccttctccc agtctgccgg aagcttctgt ccattctcac ccgcataagt
     6961 catttcgtct gaataaagta agacaagatt tgtcttaaga tttgctttag atgcttttac
     7021 agcttttaat gtttttattg cattatccaa agaaagatat gcattttccc ctgttggatt
     7081 tacagcaact tttaaagata tggtattgat accctgactt tttacatagt cgaaaatatt
     7141 gtctacggac tgactcatat aatttttata tgatttcccc cactccagac actgctgata
     7201 ataagtaaaa tccgctccta aaatagtatt cgctccaatt ccagaaatct ttttgtactt
     7261 tttaaaagca tccttactta tttctggaat ttcaatctta tctgctgacg aacttacttc
     7321 ttgcgcctta accggatgtg tatccaaact aaatccagtt gttgcaaata acatggttgc
     7381 cattgctatt gctacacatc ttttgatagc cccgctaaat ttttcaagat tcttcatttt
     7441 cattagtaga ttatgattgt ttttcataag tttctccttt cttataaata ataatatata
     7501 ttttaacata ttataatctc ttttcaagca tacactatac taaaattagt aaatttttga
     7561 tttttttcaa acatactcta tttttttatc aaatctttcc ctcgctttcc gaactcatgt
     7621 tgaaaagatt tggaaattcc ccgaaaggtt gtgattttat gtaaattctg attttactcc
     7681 agcaactata aacagccctc agagaatgaa ataatctata tttgtggcct cggagcaaag
     7741 gttggatgcg agcgctttaa gcgaacattc aacctgaagc gactaaagtc acaaatatag
     7801 atta
//
