LOCUS       c00136_gnlPro..         4284 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KNEBHBDB_136.
ACCESSION   c00136_gnlPro..
VERSION     c00136_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-29 02:12:42
            Original ID  :: gnl|Prokka|KNEBHBDB_136
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4284
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4284
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[57:2990]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      58..2990
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..4284
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4284
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             58..1602
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg136_1"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1e-47, bitscore:
                     150.2, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LSRHEQGSLHEAEGYAKSTGKLGVALVTSGPGATNAITGIADAMS
                     DSVPLLVFTGQVATSGIGKDAFQEADIVGITMPITKYNYQIRDTADIPRIIREAVHIAT
                     TGRPGPVVIDLPKDVVAKETTFINDPEINLPSYQPTLRPNEMQINKILKQLGKAKKPVI
                     VAGGGVAYSESAKELVAFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSYAANIAM
                     TDADFMIAIGCRFDDRLTGNPKTFAKNAKVVHIDVDPAEIGKIIAVDLPVVGDAKQALE
                     MLLAEPVVKNNTEKWIEKVKKDKERVRSYDKKERMVQPQAVIERIGELTDGDAVVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGVPAAIGAKIANPDKEVILFVGDGGFQMT
                     NQELAILNIYKVSIKVVMLNNHSLGMVRQWQESFYEGRTSESIFNALPDFQLMAQAYGI
                     KSYKFDNPETIDQDLEVIKEDVPMFIEVDISRKEHVLPIVPAGKSNHEMLGVKFNA"
     CDS             1635..2072
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg136_2"
                     /sec_met_domain="ALS_ss_C (E-value: 1.4e-29, bitscore:
                     90.7, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VFLTVSLECFHAVRSILRVFPLEQRENPDVSRITIIIDVNSHNEV
                     EQIIKQLNRQIDVIRIRDITDVPHLEREVILVKVSAPTSKRAEILAIIQPFRASVVDVA
                     PSSITIQMTGDAEKSEALLRVIRPYGIKNIARTGATGFTRD"
     CDS             2148..2990
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: IlvC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg136_3"
                     /sec_met_domain="IlvN (E-value: 3e-78, bitscore: 249.5,
                     seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="IlvC (E-value: 3e-45, bitscore: 142.5,
                     seeds: 295, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVQMEYEKDVKVPALDGKKIAVIGYGSQGHAHSQNLRDTGHDVI
                     IGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGNAV
                     GFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTFEEGFGVPALYAVYQDATGNAKD
                     IAMDWCKGIGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIETGFEVLTEAGYAPE
                     LAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEFGDYVSGPRVITEQVHRTHESSSC"
     CDS             2999..3172
                     /locus_tag="ctg136_4"
                     /transl_table=11
                     /translation="MVNSLTDFVNDYKAGRPKLTAYREEAANLGIEKVGAELRKAMPFV
                     GQNDDDAFKIYN"
     CDS             complement(3298..4197)
                     /locus_tag="ctg136_5"
                     /transl_table=11
                     /translation="MVNNYDWFGSVSFIDFLRDVGKYFTVNYMMSKESVKKRIETGISY
                     TEFAYQIMQGYDFYELNAKYGVTLQIGGSDQWGNMTAGTELLRRKADKSGHVITVPLIT
                     DSTGKKFGKSEGNAVWLDATKTTPYEMYQFWLNVMDDDAVRFLKIFTFLSLEEIEEIGK
                     EFDQARHQRLAQKVLAREVVTLVHGKEAYEQAVHITEQLFAGNLKALSARDLKVALNGV
                     PTYEISADENLNIVELLVNAKISPSKRQAREDVQNGAIYINGERVQDLDYTLSDTDKID
                     NEITVIRRGKKKNFVLTY"
ORIGIN
        1 ttaggcgatc gagcttgtat gatgctattt acaaaaatgc aagagactta actctttttg
       61 tctcgtcatg agcaaggaag cctgcatgaa gctgaaggtt atgctaaatc aactgggaaa
      121 cttggtgttg ctcttgtaac gagtggtccc ggtgcaacga atgctattac tgggattgca
      181 gatgctatga gtgatagtgt acctcttctg gtctttacgg gtcaggtagc aacatcaggt
      241 attggtaagg atgccttcca agaagctgac attgtcggaa ttaccatgcc aattaccaaa
      301 tacaactacc agattcgtga tacggctgat attcctcgta ttattcgaga agcggttcat
      361 atcgcaacga cagggcgtcc tggtccggtt gttatagacc ttccaaaaga tgttgtagct
      421 aaggaaacta ccttcatcaa cgatcctgaa atcaatcttc caagttacca accgacgcta
      481 agaccaaacg agatgcaaat taataaaatc ttgaaacagt tgggtaaagc taagaagccg
      541 gttattgtag ctggaggtgg agtggcttat tctgaatctg ctaaggagct agttgctttt
      601 gcagaacgct accaaattcc tgttgttaca agtcttctag gtcagggaac aattgctact
      661 agtcatcccc tctttttggg tatgggtggt atgcacggtt cgtatgcagc taatattgct
      721 atgactgatg ctgactttat gattgccata ggttgtcgtt ttgatgaccg cttgacaggt
      781 aatccaaaaa catttgctaa gaatgcaaag gttgttcata ttgatgtgga tccggctgag
      841 attggcaaga ttattgcggt agatttgcca gttgttggtg atgccaagca agctcttgaa
      901 atgctcttag cagaaccagt ggttaagaat aacactgaaa aatggattga aaaagtcaaa
      961 aaagataaag agcgtgtacg ttcttacgat aagaaagaac gtatggtaca gcctcaggcg
     1021 gttatcgagc gtatcggtga attgaccgat ggtgatgcag ttgttgtcac tgacgttggg
     1081 caacatcaaa tgtggacagc tcagtattat ccatatcaaa atgagcgtca attggtgaca
     1141 tcaggtggcc ttggaactat gggatttggt gttccagcag caattggtgc taaaattgct
     1201 aatcctgata aagaagttat cctctttgtt ggtgatggtg gtttccaaat gacgaaccaa
     1261 gaattggcta ttttaaatat ttacaaggtg tcaatcaagg ttgttatgtt gaataaccac
     1321 tcacttggta tggtacgtca atggcaagag tccttctacg aagggcgtac atcagagtct
     1381 atctttaatg ctctccctga ttttcaactg atggctcaag cttatgggat taagtcttat
     1441 aagtttgata atccagaaac gattgatcag gatttggaag ttatcaaaga ggatgtacca
     1501 atgttcatcg aggttgatat ttctcgtaaa gagcatgttt tgccaattgt tccagctggt
     1561 aagagtaatc atgaaatgtt gggggtgaag ttcaatgcgt agaatgttaa cagctaaact
     1621 tcagaaccga tcaggtgttc ttaaccgttt cactggagtg ctttcacgcc gtcaggtcaa
     1681 tattgagagt atttccgttg gagcaacggg aaaatcctga tgtttcacgt attacaatta
     1741 tcattgatgt gaattctcat aatgaggttg agcagattat taaacagctt aatcgtcaga
     1801 ttgatgtgat tcgcattcgt gacatcactg atgtacccca cttggaacgt gaggttatct
     1861 tggttaaggt gtcggcacca acttctaaac gtgctgaaat cttggcaatc attcaacctt
     1921 tccgtgcttc ggtggtggat gttgcaccaa gctctatcac tatccagatg actggagatg
     1981 ctgaaaagag tgaggcgctt ctacgagtta ttcgacctta cggtatcaag aatattgctc
     2041 gtacgggtgc tactggattt acccgtgatt aaaaaatcca ttataaattt gttaacctag
     2101 ccttaaaaag gcaattaaat attattttag aaaagagata tactactatg gcagttcaaa
     2161 tggaatatga aaaagacgta aaagtaccag cacttgatgg taaaaaaatc gccgttatcg
     2221 gttacggttc acaaggtcac gctcactcac aaaacttgcg tgatacaggt cacgatgtta
     2281 tcatcggtgt tcgtccaggt aaatcatttg ataaagctaa agaagatggt tttgatactt
     2341 acacagtagc agaagcaact aaattggctg atgttatcat gattttggct ccagacgaaa
     2401 tccaacaaga actttacgaa gcagaaatcg ctccaaatct tgaagctggt aacgctgttg
     2461 gttttgctca cggtttcaac atccactttg aatttattaa agtaccagca gatgttgatg
     2521 tatttatgtg tgcacctaaa ggaccaggtc acttggtacg tcgtacattt gaagaaggtt
     2581 ttggtgtacc tgccctttac gcagtatacc aagatgccac aggtaacgct aaagacatcg
     2641 ctatggactg gtgtaaaggt attggtgcag cacgtgtagg tcttcttgaa acaacataca
     2701 aagaagaaac tgaagaagat ttgtttggtg aacaagcagt tctttgtggt ggtttgactg
     2761 cccttatcga aactggtttt gaagtcttga ctgaagctgg atatgcacca gaattggctt
     2821 actttgaagt acttcacgaa atgaaattaa tcgttgactt gatttacgaa ggtggattca
     2881 agaaaatgcg tcaatcaatt tctaacactg ctgaattcgg tgactatgta tcaggtccac
     2941 gtgttatcac tgaacaagtt cacagaacac atgagagcag ttcttgctga tatccacaat
     3001 ggtaaattcg ctcactgact tcgttaacga ctacaaggct ggacgtccaa aacttactgc
     3061 ttaccgtgaa gaagctgcta accttggaat cgaaaaagtt ggtgctgaat tgcgtaaagc
     3121 aatgccattc gttggtcaaa acgacgatga tgcattcaaa atctataact aatgtgatag
     3181 tctcaaaaac cagcctagtg ctggtttttt gtgttcttaa gaaagtggtt aggtgttaag
     3241 cttatggctt tgtgtgtaaa aaaaatcact ccactaggga gtgagaatgg tcataaatta
     3301 gtaagttaaa acaaagtttt tcttcttacc gcgacggata acagtgattt cattgtcaat
     3361 cttatcggta tctgaaagag tgtaatccaa gtcttgcacg cgttcaccgt tgatatagat
     3421 agcaccgttt tgcacgtctt cacgtgcttg acgtttagat ggtgaaattt tagcattgac
     3481 gagaagttca acaatgttaa gattttcatc tgctgaaatc tcataggttg ggacaccatt
     3541 gagggcaact ttaaggtcac gtgctgagag ggctttaagg ttaccagcga agagttgttc
     3601 ggtgatgtga acagcttgtt cataggcttc tttaccgtga accaaagtaa caacttcgcg
     3661 agccagaacc ttttgtgcta aacgttggtg acgagcttgg tcaaattctt taccgatttc
     3721 ttcgatttct tcaagtgaca agaaagtgaa aattttcaag aaacgaacgg catcatcgtc
     3781 cataacgtta agccagaatt ggtacatttc ataaggagtt gtcttagtag cgtcaagcca
     3841 gacagcgttg ccttcagatt tgccgaattt tttaccagtt gaatctgtga taagtggaac
     3901 tgtgattacg tgtccagatt tgtccgcttt acgacgcaag agctcagtac ctgctgtcat
     3961 gttaccccat tggtcagaac caccaatttg tagagttacg ccatatttag cattgagttc
     4021 atagaagtcg taaccttgca taatttggta agcgaactca gtgtaagaga tacctgtttc
     4081 gatacgtttc ttaacagatt ccttgctcat catgtagtta actgtgaagt atttaccgac
     4141 atcacgcaag aaatcgatga aactaactga accaaaccag tcatagttat tgaccataac
     4201 ggctttgtta tcaccatttt caaatcgaan ggcgagagag ttggctgatt ttttctaccc
     4261 accttcaacg ttcttgctcg acca
//
