LOCUS       c00038_gnlPro..         7703 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NPDEIGMH_38.
ACCESSION   c00038_gnlPro..
VERSION     c00038_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-29 02:30:27
            Original ID  :: gnl|Prokka|NPDEIGMH_38
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7703
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7703
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[214:3465]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      215..3465
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7703
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7703
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             215..2476
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg38_1"
                     /sec_met_domain="Gly_radical (E-value: 2.4e-42, bitscore:
                     132.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.5e-245, bitscore:
                     804.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQLDETKVPNYWEGFNGGDWQEEINVRDFIEHNLNQYDGDESFL
                     AGPTEATTVLNNQVLNLKKQERANGGVLDADNNIPSTITSHGPGYLNKDLEKIVGVQTD
                     KPFKRAFMPFGGIRMAEDALKSYGFETDANEHKIFTEYRKTHNQGVFDAYTPEMRKARH
                     YKIVTGLPDAYGRGRIVSDFPRIAIYGIDRLMAEKFNDYNLTGDGEMTDDVIKLREEIN
                     EQYRALNDMKKMAKEYGYDISRPAANAQEAVQWIYFGYLAAVKTQNGAAMSVGRIDTVI
                     DAYIQRDMRLGKLNEEQAQELIDHLVMKLRMVRFIRTEEYNSLFSGDPIWATLSLAGMG
                     NDGRHHVTKTAFRFLKTLDNMGAAPEPNITLLWSERLPEGFKRYATEVSIQSSTIQYEN
                     DDLMRNEWGTDYYGIACCVSAQPIADGVQYFGARANLAKTVLYAINGGKDEIQEAQVGP
                     EYEPITSDYIDYDEFMKKFDKMMDWLADVYVNALNVIHYMHDKYYYEAAQLALKDTRLN
                     RTFATGISGLSHAVDSISAIKYGHVKAIRDENGVAIDFVADNDDYPRYGNNDDRADNIA
                     KWLVKTFYNKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPA
                     YGAEKNGALASLMSTAKIPYHYATDGISNTFGVTPNTLGHDDETRKDTLVHMVDGYMEN
                     SGMHLNINVFNKETLIDAQKHPEEYPTLTVRVSGYCVYFADLTKEQQDDVIARTFFDEM
                     "
     CDS             2614..3465
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 66.6;
                     E-value: 9e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg38_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.8e-27, bitscore:
                     84.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLIDQSQSPLNILEQVGQDHEGPIKGYVHSVESFGSVDGPGIRF
                     VVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILADAQRYKAFWGEQGGITCSGGESLV
                     QIDFILELFTKAKELGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAE
                     HKKLTGFPNENILDMVQYMSDHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKRVVQ
                     KVEILPYHTLGVKKYHELGITYPLEGIEPPSADRVKNAENLLHVEDYTGWQSWRPQPAN
                     KQ"
     CDS             3587..4555
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     55.5; E-value: 1.4e-17)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg38_3"
                     /sec_met_domain="LysR_substrate (E-value: 1.5e-27,
                     bitscore: 85.0, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 1.3e-18, bitscore: 55.5,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKQERIFSSKTLTYFLQLAETMNYTQSAQLLGITQPALTQQIK
                     KLERTVGAPLFYSVGKKLHLSDAGRTMLKATHEIYEILNQTTDEIQQSTSATVGKINIG
                     LLSSIEDSAFNTFIAHYYDLNPEVEITLRMMTRHEIWEALENNQIDLAIMNLPDETIKN
                     WKPYASKKILDEQLLFLHHDNSLANRKRLHFKDTLAEPWVTYPDDYFLGQLLHEAYKNQ
                     MVDFPHVVAHFTTPYQLTQFVNHTGVNTALPTSFVAAHADQIKAHAVAFEPAIRFETSF
                     VYRKEKADIPRIANFFKTFDAHLSEKDYLSRVSENSGKIER"
     CDS             4585..5517
                     /locus_tag="ctg38_4"
                     /transl_table=11
                     /translation="MTTLIFGHQNPDTDAITSAMSWAEFQKQAGNTDVEAVALGEPNDE
                     TKFVLDHFKVAAPRVIKTAANETGHVMLVDHNEFQQSVADIEDVQIDSVVDHHRIANFH
                     TASPLYYRAEPVGSTNTVIFELFQEHGYEISPTLAGLMASGLISDTLLLKSPTATDKDK
                     KILPEMAKIAGIDLQSYGLAMLKAGTNLAAKSDMDIIEGDAKSFTMGGKSIRIGQVNTV
                     DLDDIYARQDELVAAMTKEAQAKGYDDFLLIATDILNSNSTGFAVGADRDKVAAAFNEK
                     FDANDRLALPGVVSRKKQAVPPMQGAFEG"
     CDS             6396..7508
                     /locus_tag="ctg38_5"
                     /transl_table=11
                     /translation="LSTQNYPKLIQAMLPSLPDYIQDYYHSQKAIPIADSTLYQYLHFY
                     QEFLHWLISSRVTDAVSPQNVPLETLERLSLRDAQAFVAYLLERPSKTHPNKRMTRRSV
                     ALRLVGIKALYRFLTEESEPNADGEPYFYRNVWNKVKLKTHAETTTYRNHKLQEMLFVD
                     GEDARFLQWLDQQYAQQLPAKPRAYFEATKERNLAIIALLFGSGVRVSELVNMNLEDLN
                     MDRHTVQVVRKGNFQDRVNFAEWIDPYLTAYLKSRTAMIGHQKPTSPLFVTQIGQTVNR
                     IRQNTIEAFFKRYTTAYGRPSTPHKARHTLGTNIYTVTKDVQQVADQLGQTTTSATDLY
                     INLSDKSGKAALREVSETAAKAVDQHPQQP"
     CDS             complement(7535..7702)
                     /locus_tag="ctg38_6"
                     /transl_table=11
                     /translation="ERNERNERNVTYDVRRTTYVRTRQTEQQLFYMPTNIKKAATVVSL
                     DTVTLISAAT"
ORIGIN
        1 agtgagaatt tttttattta ttatatgaca gtacggttgt gcacaactta tcaagctaat
       61 gattaagctt tttttatgaa acaggaaagt acaccgaaaa aacagcaaaa cgcctgttat
      121 atcagcgata aaacgcttgc atagatttgc cttcgtgtta tactgagttt gtaagaactg
      181 tcacatctgt aattcacttt aggaggcaat acctatgaaa cagcttgacg aaaccaaagt
      241 tcctaattat tgggaaggct ttaacggcgg cgactggcaa gaagaaatta acgtgcgcga
      301 ttttatcgag cataacctga accaatatga tggggacgag agtttccttg ctggccccac
      361 tgaagcaacg accgttttaa ataatcaggt tttgaatctg aaaaagcaag aacgtgccaa
      421 tggcggcgta ctggatgcag ataacaatat tccatcgacc attacttctc atggccccgg
      481 ttatctgaat aaggatctcg aaaagattgt cggtgtccaa accgataagc cgtttaagcg
      541 agcattcatg ccatttggcg gtatccggat ggctgaggat gctttgaaat cctatggctt
      601 cgaaaccgat gctaatgagc acaagatctt taccgaatat cgcaagacgc ataaccaagg
      661 tgtttttgat gcttatacgc cggaaatgcg caaagcccgc cactataaga ttgtgaccgg
      721 cttaccggat gcttatggtc gtggtcggat cgtttcggac ttccctcgaa ttgctattta
      781 cggaattgac cggctgatgg ctgagaagtt caacgattac aacctgaccg gcgatggcga
      841 gatgacagat gatgttatca aactgcgcga agaaattaac gaacagtatc gtgccttgaa
      901 tgacatgaag aagatggcca aagaatacgg ctatgacatt tcccgcccag ctgctaatgc
      961 tcaagaagct gttcagtgga tctacttcgg ttacctggca gccgttaaga cccaaaatgg
     1021 tgcagcaatg tctgtcggcc gtatcgatac agtgattgat gcctatattc aacgggatat
     1081 gcgtttgggc aagctgaatg aagagcaggc acaagaactc atcgatcatt tggttatgaa
     1141 gttgcggatg gttcgtttca ttcggactga agaatacaac tcactgttct ccggcgatcc
     1201 gatctgggca acactgtcct tggcagggat gggtaatgat ggccgtcatc atgttaccaa
     1261 gaccgcgttc cgcttcctga agaccttgga caacatgggc gcggcaccgg aaccaaacat
     1321 caccttgttg tggagcgaac gtcttcctga agggttcaaa cgttatgcta ctgaagtgtc
     1381 catccaaagt tcaaccatcc aatacgaaaa cgatgatctg atgcgcaacg aatggggaac
     1441 cgactattac ggcattgctt gctgtgtttc cgcacagcca attgcggatg gcgttcagta
     1501 cttcggggcg cgagcaaacc tagccaagac tgtgctgtat gcgatcaacg gtggtaaaga
     1561 cgaaattcag gaagcacagg tgggacctga atacgagccg atcaccagcg actatatcga
     1621 ctatgacgag ttcatgaaga agttcgacaa gatgatggac tggttagcag atgtctatgt
     1681 caatgcactg aatgttattc actacatgca tgacaagtac tactatgaag ctgcacagtt
     1741 ggcattgaag gacacgcgtc tgaatcggac gtttgctacc gggatttccg gtttgtcaca
     1801 tgcggttgat tccatcagtg ccatcaagta tggccacgtg aaggcaattc gggatgaaaa
     1861 tggcgttgca atcgactttg ttgctgacaa tgatgactat ccgcgttatg gtaacaatga
     1921 tgaccgggca gacaacattg ctaagtggtt ggttaagacc ttctacaaca agatgaatac
     1981 acatcatctg tatcgcggtg ctaagctcag taccagtgtt ctgaccatta catcaaatgt
     2041 ggtttatggt aagaataccg ggacaacacc aaacggccgt cagaagggcg aaccattctc
     2101 acctggtgcc aacccagcgt atggcgctga aaagaacggt gctttagctt ccttgatgtc
     2161 aaccgccaag atcccatatc actatgcaac agatgggatc agcaatacct ttggggtaac
     2221 accgaacacc ttaggccatg acgatgaaac tcgtaaggac accttggttc acatggttga
     2281 cggctacatg gaaaatagcg gcatgcacct taacatcaac gtcttcaata aagaaacgtt
     2341 gattgatgcc cagaagcacc cagaagaata tccaaccttg actgttcgag tttccggtta
     2401 ctgcgtctac tttgcagacc tgaccaaaga acagcaagac gatgttatcg ctcggacctt
     2461 cttcgacgaa atgtaatcaa gtcaatctat tcgcaaaaca ggagatcatt cctgttttgt
     2521 caaaagacgg caacgatgct gttgccgttt tttgacaaac tgaaatgaaa atcgttgaaa
     2581 attaggtatt tatttgcact ggaggcggca tagatgaaac ttatcgatca atcacaaagc
     2641 ccgttaaata ttttggaaca agtgggacag gatcatgaag gcccaataaa gggatacgtt
     2701 cattcagtcg aaagctttgg ttcggttgat ggtcccggga ttcggtttgt cgtctttatg
     2761 caaggatgca ggatgcggtg ccagtactgt cacaaccctg acacctggaa tatcggtgtg
     2821 ggcgaggaaa tgaccgctga tcagatcctg gcagatgcac aacgatataa agccttctgg
     2881 ggtgagcaag gggggattac ctgtagcggt ggcgaaagtc tcgtccaaat tgattttatc
     2941 ctggaacttt ttaccaaagc taaggaactg ggtatctcaa cttgtttgga tacttcgggt
     3001 ggtccattca cccgcgatca gccttggttt ggtcaatttg agaaactgat ggccgtcacg
     3061 gatatttcgt tggttgatat caagcacatt gattccgcgg aacacaaaaa gctgaccggt
     3121 ttcccaaacg aaaacatttt ggatatggtt cagtatatgt cggatcatgg cgatgatatg
     3181 tggattcgcc acgtacttgt gccagaacga accgactttg atccttatct caaacgcttg
     3241 ggagattata ttgccacttt ggataaacgc gtggttcaga aagtggaaat tcttccgtat
     3301 catacgttag gggttaagaa gtaccatgaa ctaggtatta cgtatccgct ggaaggtatt
     3361 gagccgcctt cagctgatcg ggtcaagaat gcagagaact tgctacacgt tgaggactat
     3421 actggatggc aaagttggcg accgcagcca gctaacaagc agtgatttag ttgaaactta
     3481 acgtaacgat taaaaacgca gcaaatccgt tgcgtttttt ttaaatgcca cttatgatga
     3541 tggttgagta acgttatcaa attataaacg aaagggaagc cgccctatga agacgaaaca
     3601 agagcgcatt ttttcctcca aaactctcac gtattttttg caattagcag aaaccatgaa
     3661 ctacacccag tcagcccagc tgttgggaat tacccagccg gcactgacgc agcaaatcaa
     3721 gaaattggaa cgaacagtgg gggcaccatt gttttattcc gttggtaaga agttgcattt
     3781 atccgatgcc ggtcgcacga tgttgaaggc gacacacgag atttatgaaa ttttgaacca
     3841 gacgactgac gagattcagc aaagcaccag tgccacagtc ggtaagatta atattggctt
     3901 gttaagttca attgaagaca gtgcgtttaa tacttttatc gcgcattatt acgaccttaa
     3961 tcccgaagtc gaaatcacgt tgcgaatgat gacacgtcat gagatctggg aagcgttgga
     4021 gaacaaccag attgatttgg ccatcatgaa cctgcctgat gagacgatta agaactggaa
     4081 gccgtatgcc agcaagaaga ttcttgatga acagttattg ttcctgcatc atgataattc
     4141 cttggctaat cgcaaacgcc tgcatttcaa agatacgctg gctgagccgt gggtaactta
     4201 tccagacgac tatttcctgg gtcagttgtt gcatgaggca tataagaatc agatggttga
     4261 ttttccgcat gttgtggcgc attttaccac gccgtatcag ttaactcagt ttgtcaacca
     4321 taccggcgtt aacaccgcgt taccaaccag ttttgttgcc gctcatgctg atcaaatcaa
     4381 ggcgcatgcc gttgcctttg aaccagcgat tcgctttgag acctcgtttg tgtatcgcaa
     4441 ggaaaaagct gatattccgc ggattgccaa cttctttaag acgtttgacg cgcatttatc
     4501 cgagaaagat tatctatcgc gggtcagcga aaacagtggt aaaattgaac ggtaaactat
     4561 tcagctgagg agcgatcaga taacatgacg actttgattt ttggacatca aaaccctgat
     4621 acagacgcga ttaccagcgc catgagttgg gctgaatttc agaagcaggc aggtaatacc
     4681 gatgttgaag cagtagcact cggtgaacca aacgatgaaa ccaagtttgt attggatcat
     4741 tttaaggttg ctgcgccgcg ggtcattaaa acagccgcaa atgaaaccgg gcacgtaatg
     4801 ttggttgatc ataacgaatt ccagcaaagc gttgctgata tcgaagacgt gcaaatcgat
     4861 tcagtggttg atcaccaccg cattgccaac ttccatacgg cgtcaccact atattaccgg
     4921 gcagaaccag tgggcagtac caacacggtg attttcgagt tgttccagga acacggctat
     4981 gaaatttcac caaccctggc aggcttaatg gcgtctggct tgatttcgga taccctgctt
     5041 ctgaagtcac cgactgcaac cgacaaggac aaaaagatct tgccggaaat ggctaaaatc
     5101 gctggcattg acctgcaatc atacggtttg gctatgttga aggcgggcac caatctagct
     5161 gccaagtcgg atatggatat cattgaaggc gatgcgaaga gctttacgat gggcggtaag
     5221 tctatccgta ttggacaagt gaatacagtt gatcttgatg atatttatgc gcgtcaggat
     5281 gaattggtgg ccgctatgac caaagaagca caggcgaagg gctatgatga cttcctgtta
     5341 attgcaaccg atattttgaa cagcaattca acgggctttg ctgttggcgc agatcgtgac
     5401 aaagtggccg cagcattcaa cgaaaagttt gatgccaatg accggttggc actgcctggc
     5461 gtggtttcac gtaagaagca ggccgtaccg ccaatgcaag gtgcgtttga aggttaattt
     5521 tgaacttaat aattgatttt ataaatcgca gccgtttgtg gttgcgattt ttttgtgctc
     5581 aaaaactttg ataaacataa aatatcgaat ttttagatct gaaggtgcaa aaatgtgatg
     5641 attggcgaaa tctttcagta aggatagttc gaatttaaaa cgaggtgcaa aaatcgcagc
     5701 cctataacta cttcgtataa tatatattat gtaaactaga atggagattt tgagtcgttg
     5761 actttgacgt gacttatttg gtgttcaaat ggcaaaacga ttgatgcaag tcccctcacc
     5821 tgacagacag ctcaaaaact tttgagagaa gcaaatcaat tgtacggatg tgagcggtgc
     5881 gctttatttg ttttttctgg tttgaaaatg accattcgtt gctaaatata tgtaaattca
     5941 gcattaatca acgcagattt gtagaaagta tcattttgag tcataaacag cattcaagtc
     6001 gcaaatgttg ctgtcagctg aattctggat caaaatacga aaattgattt gattgcagcc
     6061 aatgaatcgt tggttaaact gttgttaaag cggcgccgtg gaatcaggac atcagcacag
     6121 atgtcctgat cgtgaagcaa attcaaattt gaaatgcaac taagtttagt gtcaaatgac
     6181 agctcaacaa atttggtgat ttagctggct tgatgattag aagttcattt acctttggat
     6241 aatactaaaa ctttattcag ttctacactg gtgctttttt cggcgcatat cgttgcgaat
     6301 taccgataat ttcgtcatta tccctcgcca cgcgctggtt ttttcgcgta tactgaaatt
     6361 atccaaaaga aaacatatgt tcgaggtgac tttatttgtc gacacagaac tatcctaaat
     6421 taatacaagc catgctacct tccctgccgg actatattca ggattactat cacagccaaa
     6481 aagcaattcc catcgcggac tctacccttt accagtacct gcacttttat caggaatttc
     6541 tgcattggct tatctccagt cgggtcacgg atgccgtttc gccacagaat gtgcctttgg
     6601 aaaccttaga gcggctttcc ttgcgtgatg cgcaggcgtt tgtggcgtat ttacttgagc
     6661 ggccgtcgaa aacccatccg aataaacgca tgacgcggcg cagtgtggcg ttacgactgg
     6721 tcgggatcaa ggccctttat cggttcttaa ccgaagaaag cgaaccaaat gcagacggtg
     6781 agccttattt ttaccgcaat gtttggaata aggtgaaact taagacccat gcggagacga
     6841 cgacttaccg taatcataaa ctgcaggaaa tgttgtttgt agatggcgaa gatgcgcgct
     6901 ttttacagtg gctggatcag caatatgctc agcagctgcc agcgaaaccg cgcgcttatt
     6961 ttgaggcgac gaaggaacga aatttggcga tcattgcgct gctgtttggc tccggcgtgc
     7021 gggttagtga attggtcaat atgaatttgg aagatctcaa tatggatcgt catactgtgc
     7081 aggtggtgcg taaaggcaat tttcaggatc gcgttaactt tgctgaatgg attgatcctt
     7141 atttaacggc ttatttgaag tcccgaaccg cgatgattgg gcaccagaag cctacttctc
     7201 cgctatttgt gacgcaaatc ggtcaaacgg tgaaccgcat tcgccaaaat acgattgaag
     7261 cattcttcaa acgctataca accgcttatg gccgcccatc aacaccacat aaagcacgcc
     7321 ataccttggg aaccaatatt tataccgtca ccaaggatgt gcagcaagtt gccgatcaac
     7381 tcggacaaac gacgaccagc gcgacggatc tgtacatcaa tcttagcgat aaaagtggaa
     7441 aagcggcctt acgcgaagtt tcggaaactg ccgccaaagc cgtggatcaa catccccagc
     7501 aaccataatg tcggcggtta gttgtctggt taagtcatgt tgcggcgcta attaacgtca
     7561 cagtatccag tgagacaaca gtggcggctt ttttgatgtt ggttggcata taaaaaagct
     7621 gctgctctgt ctgtcgtgta cgtacgtacg tcgtacgtcg tacgtcgtac gttacgttac
     7681 gttcgttacg ttcgttacgt tcg
//
