LOCUS       c00035_gnlPro..         7603 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EIOCMOGA_35.
ACCESSION   c00035_gnlPro..
VERSION     c00035_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-29 02:15:23
            Original ID  :: gnl|Prokka|EIOCMOGA_35
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7603
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7603
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[192:3443]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      193..3443
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7603
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7603
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             193..2454
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg35_1"
                     /sec_met_domain="Gly_radical (E-value: 2e-42, bitscore:
                     132.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.3e-245, bitscore:
                     804.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQLDETKVPNYWEGFNGGDWQEEINVRDFIEHNLNQYDGDESFL
                     AGPTEATTVLNNQVLNLKKQERANGGVLDADNNIPSTITSHGPGYLNKDLEKIVGVQTD
                     KPFKRAFMPFGGIRMAEDALKSYGFETDANEHKIFTEYRKTHNQGVFDAYTPEMRKARH
                     YKIVTGLPDAYGRGRIVSDFPRIAIYGIDRLMAEKFNDYNLTGDGEMTDDVIKLREEIN
                     EQYRALNDMKKMAKEYGYDISRPAANAQEAVQWIYFGYLAAVKTQNGAAMSVGRIDTVI
                     DAYIQRDMRLGKLNEEQAQELIDHLVMKLRMVRFIRTEEYNSLFSGDPIWATLSLAGMG
                     NDGRHHVTKTAFRFLKTLDNMGAAPEPNITLLWSERLPEGFKRYATEVSIQSSTIQYEN
                     DDLMRNEWGTDYYGIACCVSAQPIADGVQYFGARANLAKTVLYAINGGKDEIQEAQVGP
                     EYEPITSDYIDYDEFMKKFDKMMDWLADVYVNALNVIHYMHDKYYYEAAQLALKDTRLN
                     RTFATGISGLSHAVDSISAIKYGHVKAIRDENGVAIDFVADNDDYPRYGNNDDRADNIA
                     KWLVKTFYNKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPA
                     YGAEKNGALASLMSTAKIPYHYATDGISNTFGVTPNTLGHDDETRKDTLVHMVDGYMEN
                     SGMHLNINVFNKETLIDAQKHPEEYPTLTVRVSGYCVYFADLTKEQQDDVIARTFFDEM
                     "
     CDS             2592..3443
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 66.6;
                     E-value: 9e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg35_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.4e-27, bitscore:
                     84.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLIDQSQSPLNILEQVGQDHEGPIKGYVHSVESFGSVDGPGIRF
                     VVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILADAQRYKAFWGEQGGITCSGGESLV
                     QIDFILELFTKAKELGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAE
                     HKKLTGFPNENILDMVQYMSDHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKRVVQ
                     KVEILPYHTLGVKKYHELGITYPLEGIEPPSADRVKNAENLLHVEDYTGWQSWRPQPAN
                     KQ"
     CDS             3565..4533
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     55.5; E-value: 1.4e-17)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg35_3"
                     /sec_met_domain="LysR_substrate (E-value: 1.3e-27,
                     bitscore: 85.0, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 1.1e-18, bitscore: 55.5,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKQERIFSSKTLTYFLQLAETMNYTQSAQLLGITQPALTQQIK
                     KLERTVGAPLFYSVGKKLHLSDAGRTMLKATHEIYEILNQTTDEIQQSTSATVGKINIG
                     LLSSIEDSAFNTFIAHYYDLNPEVEITLRMMTRHEIWEALENNQIDLAIMNLPDETIKN
                     WKPYASKKILDEQLLFLHHDNSLANRKRLHFKDTLAEPWVTYPDDYFLGQLLHEAYKNQ
                     MVDFPHVVAHFTTPYQLTQFVNHTGVNTALPTSFVAAHADQIKAHAVAFEPAIRFETSF
                     VYRKEKADIPRIANFFKTFDAHLSEKDYLSRVSENSGKIER"
     CDS             4563..5495
                     /locus_tag="ctg35_4"
                     /transl_table=11
                     /translation="MTTLIFGHQNPDTDAITSAMSWAEFQKQAGNTDVEAVALGEPNDE
                     TKFVLDHFKVAAPRVIKTAANETGHVMLVDHNEFQQSVADIEDVQIDSVVDHHRIANFH
                     TASPLYYRAEPVGSTNTVIFELFQEHGYEISPTLAGLMASGLISDTLLLKSPTATDKDK
                     KILPEMAKIAGIDLQSYGLAMLKAGTNLAAKSDMDIIEGDAKSFTMGGKSIRIGQVNTV
                     DLDDIYARQDELVAAMTKEAQAKGYDDFLLIATDILNSNSTGFAVGADRDKVAAAFNEK
                     FDANDRLALPGVVSRKKQAVPPMQGAFEG"
     CDS             6374..7486
                     /locus_tag="ctg35_5"
                     /transl_table=11
                     /translation="LSTQNYPKLIQAMLPSLPDYIQDYYHSQKAIPIADSTLYQYLHFY
                     QEFLHWLISSRVTDAVSPQNVPLETLERLSLRDAQAFVAYLLERPSKTHPNKRMTRRSV
                     ALRLVGIKALYRFLTEESEPNADGEPYFYRNVWNKVKLKTHAETTTYRNHKLQEMLFVD
                     GEDARFLQWLDQQYAQQLPAKPRAYFEATKERNLAIIALLFGSGVRVSELVNMNLEDLN
                     MDRHTVQVVRKGNFQDRVNFAEWIDPYLTAYLKSRTAMIGHQKPTSPLFVTQIGQTVNR
                     IRQNTIEAFFKRYTTAYGRPSTPHKARHTLGTNIYTVTKDVQQVADQLGQTTTSATDLY
                     INLSDKSGKAALREVSETAAKAVDQHPQQP"
ORIGIN
        1 atatgacagt acggttgtgc acaacttatc aagctaatga ttaagctttt tttatgaaac
       61 aggaaagtac accgaaaaaa cagcaaaacg cctgttatat cagcgataaa acgcttgcat
      121 agatttgcct tcgtgttata ctgagtttgt aagaactgtc acatctgtaa ttcactttag
      181 gaggcaatac ctatgaaaca gcttgacgaa accaaagttc ctaattattg ggaaggcttt
      241 aacggcggcg actggcaaga agaaattaac gtgcgcgatt ttatcgagca taacctgaac
      301 caatatgatg gggacgagag tttccttgct ggccccactg aagcaacgac cgttttaaat
      361 aatcaggttt tgaatctgaa aaagcaagaa cgtgccaatg gcggcgtact ggatgcagat
      421 aacaatattc catcgaccat tacttctcat ggccccggtt atctgaataa ggatctcgaa
      481 aagattgtcg gtgtccaaac cgataagccg tttaagcgag cattcatgcc atttggcggt
      541 atccggatgg ctgaggatgc tttgaaatcc tatggcttcg aaaccgatgc taatgagcac
      601 aagatcttta ccgaatatcg caagacgcat aaccaaggtg tttttgatgc ttatacgccg
      661 gaaatgcgca aagcccgcca ctataagatt gtgaccggct taccggatgc ttatggtcgt
      721 ggtcggatcg tttcggactt ccctcgaatt gctatttacg gaattgaccg gctgatggct
      781 gagaagttca acgattacaa cctgaccggc gatggcgaga tgacagatga tgttatcaaa
      841 ctgcgcgaag aaattaacga acagtatcgt gccttgaatg acatgaagaa gatggccaaa
      901 gaatacggct atgacatttc ccgcccagct gctaatgctc aagaagctgt tcagtggatc
      961 tacttcggtt acctggcagc cgttaagacc caaaatggtg cagcaatgtc tgtcggccgt
     1021 atcgatacag tgattgatgc ctatattcaa cgggatatgc gtttgggcaa gctgaatgaa
     1081 gagcaggcac aagaactcat cgatcatttg gttatgaagt tgcggatggt tcgtttcatt
     1141 cggactgaag aatacaactc actgttctcc ggcgatccga tctgggcaac actgtccttg
     1201 gcagggatgg gtaatgatgg ccgtcatcat gttaccaaga ccgcgttccg cttcctgaag
     1261 accttggaca acatgggcgc ggcaccggaa ccaaacatca ccttgttgtg gagcgaacgt
     1321 cttcctgaag ggttcaaacg ttatgctact gaagtgtcca tccaaagttc aaccatccaa
     1381 tacgaaaacg atgatctgat gcgcaacgaa tggggaaccg actattacgg cattgcttgc
     1441 tgtgtttccg cacagccaat tgcggatggc gttcagtact tcggggcgcg agcaaaccta
     1501 gccaagactg tgctgtatgc gatcaacggt ggtaaagacg aaattcagga agcacaggtg
     1561 ggacctgaat acgagccgat caccagcgac tatatcgact atgacgagtt catgaagaag
     1621 ttcgacaaga tgatggactg gttagcagat gtctatgtca atgcactgaa tgttattcac
     1681 tacatgcatg acaagtacta ctatgaagct gcacagttgg cattgaagga cacgcgtctg
     1741 aatcggacgt ttgctaccgg gatttccggt ttgtcacatg cggttgattc catcagtgcc
     1801 atcaagtatg gccacgtgaa ggcaattcgg gatgaaaatg gcgttgcaat cgactttgtt
     1861 gctgacaatg atgactatcc gcgttatggt aacaatgatg accgggcaga caacattgct
     1921 aagtggttgg ttaagacctt ctacaacaag atgaatacac atcatctgta tcgcggtgct
     1981 aagctcagta ccagtgttct gaccattaca tcaaatgtgg tttatggtaa gaataccggg
     2041 acaacaccaa acggccgtca gaagggcgaa ccattctcac ctggtgccaa cccagcgtat
     2101 ggcgctgaaa agaacggtgc tttagcttcc ttgatgtcaa ccgccaagat cccatatcac
     2161 tatgcaacag atgggatcag caataccttt ggggtaacac cgaacacctt aggccatgac
     2221 gatgaaactc gtaaggacac cttggttcac atggttgacg gctacatgga aaatagcggc
     2281 atgcacctta acatcaacgt cttcaataaa gaaacgttga ttgatgccca gaagcaccca
     2341 gaagaatatc caaccttgac tgttcgagtt tccggttact gcgtctactt tgcagacctg
     2401 accaaagaac agcaagacga tgttatcgct cggaccttct tcgacgaaat gtaatcaagt
     2461 caatctattc gcaaaacagg agatcattcc tgttttgtca aaagacggca acgatgctgt
     2521 tgccgttttt tgacaaactg aaatgaaaat cgttgaaaat taggtattta tttgcactgg
     2581 aggcggcata gatgaaactt atcgatcaat cacaaagccc gttaaatatt ttggaacaag
     2641 tgggacagga tcatgaaggc ccaataaagg gatacgttca ttcagtcgaa agctttggtt
     2701 cggttgatgg tcccgggatt cggtttgtcg tctttatgca aggatgcagg atgcggtgcc
     2761 agtactgtca caaccctgac acctggaata tcggtgtggg cgaggaaatg accgctgatc
     2821 agatcctggc agatgcacaa cgatataaag ccttctgggg tgagcaaggg gggattacct
     2881 gtagcggtgg cgaaagtctc gtccaaattg attttatcct ggaacttttt accaaagcta
     2941 aggaactggg tatctcaact tgtttggata cttcgggtgg tccattcacc cgcgatcagc
     3001 cttggtttgg tcaatttgag aaactgatgg ccgtcacgga tatttcgttg gttgatatca
     3061 agcacattga ttccgcggaa cacaaaaagc tgaccggttt cccaaacgaa aacattttgg
     3121 atatggttca gtatatgtcg gatcatggcg atgatatgtg gattcgccac gtacttgtgc
     3181 cagaacgaac cgactttgat ccttatctca aacgcttggg agattatatt gccactttgg
     3241 ataaacgcgt ggttcagaaa gtggaaattc ttccgtatca tacgttaggg gttaagaagt
     3301 accatgaact aggtattacg tatccgctgg aaggtattga gccgccttca gctgatcggg
     3361 tcaagaatgc agagaacttg ctacacgttg aggactatac tggatggcaa agttggcgac
     3421 cgcagccagc taacaagcag tgatttagtt gaaacttaac gtaacgatta aaaacgcagc
     3481 aaatccgttg cgtttttttt aaatgccact tatgatgatg gttgagtaac gttatcaaat
     3541 tataaacgaa agggaagccg ccctatgaag acgaaacaag agcgcatttt ttcctccaaa
     3601 actctcacgt attttttgca attagcagaa accatgaact acacccagtc agcccagctg
     3661 ttgggaatta cccagccggc actgacgcag caaatcaaga aattggaacg aacagtgggg
     3721 gcaccattgt tttattccgt tggtaagaag ttgcatttat ccgatgccgg tcgcacgatg
     3781 ttgaaggcga cacacgagat ttatgaaatt ttgaaccaga cgactgacga gattcagcaa
     3841 agcaccagtg ccacagtcgg taagattaat attggcttgt taagttcaat tgaagacagt
     3901 gcgtttaata cttttatcgc gcattattac gaccttaatc ccgaagtcga aatcacgttg
     3961 cgaatgatga cacgtcatga gatctgggaa gcgttggaga acaaccagat tgatttggcc
     4021 atcatgaacc tgcctgatga gacgattaag aactggaagc cgtatgccag caagaagatt
     4081 cttgatgaac agttattgtt cctgcatcat gataattcct tggctaatcg caaacgcctg
     4141 catttcaaag atacgctggc tgagccgtgg gtaacttatc cagacgacta tttcctgggt
     4201 cagttgttgc atgaggcata taagaatcag atggttgatt ttccgcatgt tgtggcgcat
     4261 tttaccacgc cgtatcagtt aactcagttt gtcaaccata ccggcgttaa caccgcgtta
     4321 ccaaccagtt ttgttgccgc tcatgctgat caaatcaagg cgcatgccgt tgcctttgaa
     4381 ccagcgattc gctttgagac ctcgtttgtg tatcgcaagg aaaaagctga tattccgcgg
     4441 attgccaact tctttaagac gtttgacgcg catttatccg agaaagatta tctatcgcgg
     4501 gtcagcgaaa acagtggtaa aattgaacgg taaactattc agctgaggag cgatcagata
     4561 acatgacgac tttgattttt ggacatcaaa accctgatac agacgcgatt accagcgcca
     4621 tgagttgggc tgaatttcag aagcaggcag gtaataccga tgttgaagca gtagcactcg
     4681 gtgaaccaaa cgatgaaacc aagtttgtat tggatcattt taaggttgct gcgccgcggg
     4741 tcattaaaac agccgcaaat gaaaccgggc acgtaatgtt ggttgatcat aacgaattcc
     4801 agcaaagcgt tgctgatatc gaagacgtgc aaatcgattc agtggttgat caccaccgca
     4861 ttgccaactt ccatacggcg tcaccactat attaccgggc agaaccagtg ggcagtacca
     4921 acacggtgat tttcgagttg ttccaggaac acggctatga aatttcacca accctggcag
     4981 gcttaatggc gtctggcttg atttcggata ccctgcttct gaagtcaccg actgcaaccg
     5041 acaaggacaa aaagatcttg ccggaaatgg ctaaaatcgc tggcattgac ctgcaatcat
     5101 acggtttggc tatgttgaag gcgggcacca atctagctgc caagtcggat atggatatca
     5161 ttgaaggcga tgcgaagagc tttacgatgg gcggtaagtc tatccgtatt ggacaagtga
     5221 atacagttga tcttgatgat atttatgcgc gtcaggatga attggtggcc gctatgacca
     5281 aagaagcaca ggcgaagggc tatgatgact tcctgttaat tgcaaccgat attttgaaca
     5341 gcaattcaac gggctttgct gttggcgcag atcgtgacaa agtggccgca gcattcaacg
     5401 aaaagtttga tgccaatgac cggttggcac tgcctggcgt ggtttcacgt aagaagcagg
     5461 ccgtaccgcc aatgcaaggt gcgtttgaag gttaattttg aacttaataa ttgattttat
     5521 aaatcgcagc cgtttgtggt tgcgattttt ttgtgctcaa aaactttgat aaacataaaa
     5581 tatcgaattt ttagatctga aggtgcaaaa atgtgatgat tggcgaaatc tttcagtaag
     5641 gatagttcga atttaaaacg aggtgcaaaa atcgcagccc tataactact tcgtataata
     5701 tatattatgt aaactagaat ggagattttg agtcgttgac tttgacgtga cttatttggt
     5761 gttcaaatgg caaaacgatt gatgcaagtc ccctcacctg acagacagct caaaaacttt
     5821 tgagagaagc aaatcaattg tacggatgtg agcggtgcgc tttatttgtt ttttctggtt
     5881 tgaaaatgac cattcgttgc taaatatatg taaattcagc attaatcaac gcagatttgt
     5941 agaaagtatc attttgagtc ataaacagca ttcaagtcgc aaatgttgct gtcagctgaa
     6001 ttctggatca aaatacgaaa attgatttga ttgcagccaa tgaatcgttg gttaaactgt
     6061 tgttaaagcg gcgccgtgga atcaggacat cagcacagat gtcctgatcg tgaagcaaat
     6121 tcaaatttga aatgcaacta agtttagtgt caaatgacag ctcaacaaat ttggtgattt
     6181 agctggcttg atgattagaa gttcatttac ctttggataa tactaaaact ttattcagtt
     6241 ctacactggt gcttttttcg gcgcatatcg ttgcgaatta ccgataattt cgtcattatc
     6301 cctcgccacg cgctggtttt ttcgcgtata ctgaaattat ccaaaagaaa acatatgttc
     6361 gaggtgactt tatttgtcga cacagaacta tcctaaatta atacaagcca tgctaccttc
     6421 cctgccggac tatattcagg attactatca cagccaaaaa gcaattccca tcgcggactc
     6481 taccctttac cagtacctgc acttttatca ggaatttctg cattggctta tctccagtcg
     6541 ggtcacggat gccgtttcgc cacagaatgt gcctttggaa accttagagc ggctttcctt
     6601 gcgtgatgcg caggcgtttg tggcgtattt acttgagcgg ccgtcgaaaa cccatccgaa
     6661 taaacgcatg acgcggcgca gtgtggcgtt acgactggtc gggatcaagg ccctttatcg
     6721 gttcttaacc gaagaaagcg aaccaaatgc agacggtgag ccttattttt accgcaatgt
     6781 ttggaataag gtgaaactta agacccatgc ggagacgacg acttaccgta atcataaact
     6841 gcaggaaatg ttgtttgtag atggcgaaga tgcgcgcttt ttacagtggc tggatcagca
     6901 atatgctcag cagctgccag cgaaaccgcg cgcttatttt gaggcgacga aggaacgaaa
     6961 tttggcgatc attgcgctgc tgtttggctc cggcgtgcgg gttagtgaat tggtcaatat
     7021 gaatttggaa gatctcaata tggatcgtca tactgtgcag gtggtgcgta aaggcaattt
     7081 tcaggatcgc gttaactttg ctgaatggat tgatccttat ttaacggctt atttgaagtc
     7141 ccgaaccgcg atgattgggc accagaagcc tacttctccg ctatttgtga cgcaaatcgg
     7201 tcaaacggtg aaccgcattc gccaaaatac gattgaagca ttcttcaaac gctatacaac
     7261 cgcttatggc cgcccatcaa caccacataa agcacgccat accttgggaa ccaatattta
     7321 taccgtcacc aaggatgtgc agcaagttgc cgatcaactc ggacaaacga cgaccagcgc
     7381 gacggatctg tacatcaatc ttagcgataa aagtggaaaa gcggccttac gcgaagtttc
     7441 ggaaactgcc gccaaagccg tggatcaaca tccccagcaa ccataatgtc ggcggttagt
     7501 tgtctggtta agtcatgttg cggcgctaat taacgtcaca gtatccagtg agacaacagt
     7561 ggcggctttt ttgatgttgg ttggcatata aaaaagctgc ctg
//
