LOCUS       c00041_gnlPro..         6456 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FOBANJGK_51.
ACCESSION   c00041_gnlPro..
VERSION     c00041_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-30 02:34:09
            Original ID  :: gnl|Prokka|FOBANJGK_51
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6456
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..834
                     /locus_tag="ctg41_1"
                     /transl_table=11
                     /translation="IFTGELSTLQHLYIVTNAGNVIYRPVHEISDARWKDTGEHLSQTV
                     GLGTDEKVLAVFAFDQLDGAGSFVLGSSDGYIKQTALSDLQPQRTYKSKPMMAMKLKDP
                     AAIVTNAYFTTDQQQDVFVVSRHAYGLTFPLSDVSTVGARATGVKSMDLKPDDEVVNFI
                     LVKDPTTAKVGILTQRGAFKKMALSEVGEMSRARRGLLVLRELKRDPHRIVAMMSVDDA
                     TRLNVLTDTGKVTTLNPAQHPAGDRYSNGSFVIDTDVLGKPVFVQTKEEPADQVE"
     protocluster    1..6456
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1085:4330]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1086..4330
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6456
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6456
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             1086..3347
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg41_2"
                     /sec_met_domain="Gly_radical (E-value: 2e-42, bitscore:
                     132.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.5e-245, bitscore:
                     802.0, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQLDETKVPNYWEGFNGGDWQEEINVRDFIEHNLNQYDGDESFL
                     AGPTEATTVLNNQVLNLKKQERANGGVLDADNNIPSTITSHGPGYLNKDLEKIVGVQTD
                     KPFKRAFMPFGGIRMAEDALESYGFKTDPQEHKIFNEYRKTHNQGVFDAYTPDMRKARH
                     YKIVTGLPDAYGRGRIVSDFPRIAVYGIDRLMAEKFKDYNLTGDGEMTDDVIKLREEIN
                     EQYRALNDMKKMAKEYGYDISRPAANAQEAVQWIYFGYLAAVKTQNGAAMSVGRIDTVI
                     DAYIQRDMRLGKLNEEQAQELIDHLVMKLRMVRFIRTEEYNSLFSGDPIWATLSLAGMG
                     NDGRHHVTKTAFRFLKTLDNMGAAPEPNITLLWSERLPEGFKRYATEVSIQSSTIQYEN
                     DDLMRNEWGTDYYGIACCVSAQPIADGVQYFGARANLAKTVLYAINGGKDEIQEAQVGP
                     EYAPITSDYIDYKEFMNKFDKMMDWLADTYVNALNVIHYMHDKYYYEAAQLALKDTQLN
                     RTFATGISGLSHAVDSISAIKYGHVKAIRDENGVAVDFVADNDDYPRYGNNDDRADDIA
                     KWLVKTFYNKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPA
                     YGAEKNGALASLMSTAKIPYHYATDGISNTFGVTPNTLGHDDETRKDTLVHMVDGYMEN
                     SGMHLNINVFNKETLIDAQKHPEEYPTLTVRVSGYCVYFADLTKEQQDDVIARTFFEEM
                     "
     CDS             3479..4330
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 65.2;
                     E-value: 2.4e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg41_3"
                     /sec_met_domain="Radical_SAM (E-value: 1.3e-26, bitscore:
                     82.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLIDQSQSPLNILEQVGQDHDGPIKGYVHSVESFGSVDGPGIRF
                     VVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILADAQRYKAFWGDQGGITCSGGESLV
                     QIDFILELFTKAKALGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAE
                     HKKLTGFPNENILDMVQYMSAHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKNVVQ
                     KVEILPYHTLGVKKYHELGITYPLEGIEPPSAERVKNAENLLHVKDYTGWQSWRPKPVA
                     SN"
     CDS             4455..5423
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     60.8; E-value: 3.2e-19)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg41_4"
                     /sec_met_domain="LysR_substrate (E-value: 7.5e-28,
                     bitscore: 85.7, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 2.6e-20, bitscore: 60.8,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKQERIFSSKTLTYFLQLAETMNYTQAAQLLGITQPALTQQIK
                     KLERTVGAPLFYSVGKKLHLSDAGQTMLKSTHEIYEILNQTTDEIQQTTSATVGKINIG
                     LLSSIEDSAFIAFIAHYYQLNPEVEVTLRMMTRHEIWESLENNQIDLAIMNLPDETIKN
                     WKPYASKKIIDEQLLFLHHDDALANRKRIHFKDTLAEPWATYPDDYFLGQLLREAYKNQ
                     MVDFPHVVAHFTTPYQLTQFVNLSGVNTALPTSFVRARADQIKAYAIAFDPAIRFEISF
                     VYRKEKADIPRISNFFQTFDDYLKEKDYLSRVSENRVKLDK"
     CDS             5453..6385
                     /locus_tag="ctg41_5"
                     /transl_table=11
                     /translation="MTTLIFGHQNPDTDAITSAMSWAEFQKQAGNTDVEAVALGEPNDE
                     TKFVFDHFKVQAPRVIKTAANETDHVMLVDHNEFQQSVSDIEDVTIDSVVDHHRIANFH
                     TAAPLFYRAEPVGSTNTVIFEMFQEYGFKISPTLAGLMASGLISDTLLLKSPTATDKDK
                     KILPEMAKIAGIDLQSYGLAMLKAGTNLAAKSDMDIIEGDAKSFTMGGKSIRIGQVNTV
                     DLNDIYARQDELISAMTKEAQAKGYDDFLLIATDILDSNSTGFAVGADRDKIASAFNEQ
                     FDKDARLALPGVVSRKKQVVPPMQSTFEG"
ORIGIN
        1 attttcactg gcgaactgag tacgttgcag catctttaca tcgtgacaaa tgccggaaat
       61 gtcatttatc gaccagtgca tgaaattagt gatgcgcgtt ggaaagacac cggcgaacac
      121 ttaagccaaa cagttggcct tggcactgac gaaaaagtct tggcagtgtt tgcttttgat
      181 caactcgatg gcgctggcag ttttgtgtta gggtcaagtg acgggtacat caaacaaact
      241 gccttaagtg atttgcagcc gcaacggacg tacaagagta agccgatgat ggcgatgaag
      301 ctgaaagacc cagccgcgat tgtcaccaat gcatacttca cgactgatca gcaacaggat
      361 gtctttgtgg tcagccgcca cgcttacgga ctgacattcc cgttaagcga tgtgtcaact
      421 gtcggtgccc gggcaaccgg cgttaaaagt atggatctaa agccagacga tgaggttgtc
      481 aatttcattc tagtgaagga tccaactacg gctaaggtcg gtattttgac ccaacgtggc
      541 gccttcaaaa agatggcctt aagcgaggtc ggggaaatga gtcgtgcccg tcgtggttta
      601 ctggtcttgc gtgagcttaa gcgtgatccg catcggattg tcgcgatgat gtcagtcgat
      661 gacgctaccc gcctgaatgt tttgaccgat acgggtaagg taacgaccct taatccggca
      721 caacatccag caggtgaccg atattcgaat ggttcgtttg tcattgacac tgatgtcctt
      781 ggcaagccag tttttgttca aaccaaagaa gaaccagcag atcaagtcga ataaatggtg
      841 atcattgacg ccgccgtctg tactaataca gatggtggcg tttttgatta ttataggacg
      901 gatcagttgt gcacaattta acaaaatgcc gaaatcatgg gcgaaaagaa gcgacgaagc
      961 acacttaaaa aacaatataa cgcctattat atgggcaata aaacgcttgc atagatttgg
     1021 cggcgtgtta tactgtgttt gtaagaactg tcacatctgt aatccacttt aggaggcaaa
     1081 caactatgaa acagcttgac gaaaccaaag ttcctaatta ttgggaaggc tttaacggcg
     1141 gcgactggca agaagaaatt aatgtgcgcg attttatcga gcataacctg aaccaatatg
     1201 acggggacga aagtttcctt gctggcccca ctgaagcgac aacagtcctg aacaatcagg
     1261 ttctaaattt aaagaagcaa gaacgtgcaa atggcggtgt tttggatgct gacaacaata
     1321 ttccatctac gatcacctca cacggccctg gttatttgaa caaagatctt gaaaagattg
     1381 ttggtgttca gaccgacaag ccattcaaac gagcattcat gccatttggc ggtattcgaa
     1441 tggctgaaga tgcattggaa tcttatggct tcaagacaga tcctcaagaa cacaagattt
     1501 tcaatgaata tcgtaagacc cataaccaag gtgtctttga tgcttatacc cctgatatgc
     1561 gaaaggctcg ccattacaag atcgtgactg gcttgccaga cgcatacggc cgtggccgga
     1621 ttgtttctga tttcccacgg atcgctgttt acgggatcga tcgtttaatg gctgagaagt
     1681 tcaaggatta taacttgacc ggcgacggcg aaatgactga cgatgtaatc aaactgcgtg
     1741 aagaaatcaa tgaacaatat cgtgccttaa acgacatgaa gaagatggcc aaggaatatg
     1801 gctatgacat ctcgcgtccg gcagctaatg cgcaagaagc tgttcagtgg atctactttg
     1861 gctatctggc tgctgttaag actcaaaacg gtgccgcaat gtccgttggc cggatcgata
     1921 cggttattga tgcttatatc caacgcgata tgcgcttagg caaattgaac gaagaacagg
     1981 cacaggaact gattgatcat ttggttatga aattgcggat ggttcgtttc atccggactg
     2041 aagaatataa ctcactgttc tcaggcgatc caatctgggc aaccttgtca ttggcaggaa
     2101 tgggcaatga tggccgtcac cacgttacca agacggcttt ccgtttcctg aagactttgg
     2161 acaacatggg cgcagcacca gaaccaaaca tcaccttgct ttggagcgaa cgcttgccag
     2221 aaggcttcaa acgctatgca accgaagtat ccatccaaag ttcaaccatt caatatgaaa
     2281 atgatgacct gatgcgcaac gaatggggta ccgattacta cggtattgct tgctgtgtct
     2341 cggcacaacc aattgctgat ggcgttcaat actttggtgc ccgtgctaac ttagctaaga
     2401 cggttctgta tgcgatcaac ggtgggaaag atgaaattca ggaagcgcaa gtagggcctg
     2461 aatatgcacc gatcaccagc gattacattg attacaaaga attcatgaat aagttcgaca
     2521 agatgatgga ctggctggcc gacacgtatg tcaatgcttt gaatgtgatt cactatatgc
     2581 atgacaagta ttattatgaa gctgcacagc tggcattgaa ggatacccaa ctgaaccgga
     2641 ccttcgcaac cgggatttcc ggcctgtcac acgcggttga ttccatcagt gcaatcaagt
     2701 atggtcatgt caaggcaatt cgcgatgaaa acggtgttgc agttgacttc gttgctgaca
     2761 atgatgacta tccacgctat ggtaacaacg atgatcgcgc tgatgacatc gctaagtggt
     2821 tggttaagac cttctataac aagatgaaca cgcatcatct gtatcgcggc gcgaagttga
     2881 gtaccagtgt cctgaccatt acctccaacg tggtttatgg taagaataca gggacaacac
     2941 caaacggtcg tcaaaaaggc gaaccattct caccaggtgc caacccggca tacggcgcag
     3001 aaaagaacgg cgcattggca tccttgatgt ccaccgccaa gattccgtat cattacgcaa
     3061 ctgacggcat tagtaatacg tttggggtta ctccgaacac actgggccac gatgatgaaa
     3121 ctcgtaagga taccttggtt cacatggttg acggctatat ggaaaacagc ggcatgcatt
     3181 tgaacatcaa cgtcttcaac aaggagaccc tgattgatgc tcagaagcac ccagaagaat
     3241 acccaacatt gaccgttcgg gtttctggct actgcgtcta cttcgcagat ttgaccaagg
     3301 aacaacaaga cgatgtcatt gctcggacct tcttcgaaga aatgtaagca gcttaactca
     3361 agacaggaag ccattcctgt tttgccaaaa gacggcaacg atgctgttgc cgttttttgg
     3421 cagaatgaaa tgaaaatcaa ttgaaaacga cacatgatgt tgtagtggag gcaataagat
     3481 gaaactcatc gatcagtcac aaagcccatt aaatatctta gaacaagttg gtcaggatca
     3541 tgatggcccg atcaaagggt atgttcactc ggtcgaaagt tttggttcgg tcgatggccc
     3601 tggtattcgc ttcgttgtct ttatgcaagg gtgtcgcatg cgttgccagt attgtcacaa
     3661 ccctgacacc tggaacattg gggttggtga agaaatgacg gccgatcaaa ttttggcgga
     3721 tgcccagcgc tataaagcat tctggggtga ccaagggggt attacctgca gtggcggtga
     3781 gagtttggta caaatcgatt tcattctcga actcttcacc aaggctaagg cactgggaat
     3841 ttcgacttgc ctcgatacgt caggtggccc cttcacgcgt gaccaaccgt ggtttggcca
     3901 gtttgaaaag ctgatggctg ttactgacat ctcattagtt gatattaaac acattgattc
     3961 ggccgagcat aaaaagctca ccggttttcc taatgagaat attttggata tggtgcagta
     4021 tatgtcggcg catggtgatg acatgtggat tcgccacgtt ctggtcccgg aacgcactga
     4081 ctttgaccct tatctcaaac ggttagggga ctatattgcg acattggaca aaaacgtggt
     4141 ccaaaaagtt gagattctgc cgtatcacac gttaggcgtt aaaaagtatc acgagcttgg
     4201 cattacgtac ccgcttgaag gcatcgaacc gccgtctgcc gaacgcgtta agaatgcaga
     4261 gaatctgctg cacgttaagg attataccgg atggcaaagc tggcgtccga aaccagttgc
     4321 gagcaactga atttttagct agaacttaac gtaacgatta aaaacgcggc aaattcgttg
     4381 cgtttttttt cattgccact tatgatgatg gttgaacgat attattaaat tataaacgaa
     4441 agggaagccg ccctatgaaa acgaaacaag agcgcatttt ttcctcaaag acactaactt
     4501 attttctgca attggcagaa accatgaact acacccaagc cgcccaattg cttggcatta
     4561 cccagccagc cttaacacag cagatcaaga aattggaacg gacggtgggg gcaccgctgt
     4621 tttattctgt cgggaagaaa ctgcatttat ctgacgctgg tcagaccatg ttaaagtcaa
     4681 cccatgagat ctacgagatc ttgaaccaga caaccgacga aattcaacaa acaaccagtg
     4741 caaccgtcgg aaaaatcaat attgggttat taagctcaat tgaggatagc gcctttattg
     4801 cgttcattgc tcattactat caactgaatc ctgaagttga agtaacgcta cggatgatga
     4861 cccgtcacga aatttgggaa tccttggaaa acaaccagat tgatttggct atcatgaatc
     4921 tgccagatga gaccattaag aactggaaac catacgccag caagaaaatt attgatgaac
     4981 aattgctgtt cttgcaccac gatgacgcct tggctaaccg taagcgaatc catttcaaag
     5041 atactttagc tgaaccttgg gcaacttatc ctgacgacta tttccttgga cagcttctgc
     5101 gtgaggcgta caagaatcaa atggttgatt tcccgcacgt ggtcgcacat ttcaccacgc
     5161 catatcagtt aacccaattc gttaacttgt caggggtcaa cacggccttg ccaaccagtt
     5221 ttgttcgggc acgcgctgat caaatcaagg cctatgctat tgcgtttgat ccggccattc
     5281 gctttgagat ttcttttgtt tatcgcaaag agaaagctga cattccgcgg atctcgaact
     5341 tcttccagac ctttgacgat tatctgaagg aaaaagatta cttgtcgcgg gtcagtgaaa
     5401 accgtgttaa acttgataag taaactattc agttgaggag cgatcagata agatgacgac
     5461 tttgattttt gggcatcaga acccggacac cgacgcgatt acgagtgcca tgagctgggc
     5521 agaatttcag aaacaggcag gcaacacgga tgttgaagcg gtagcattag gcgaaccgaa
     5581 cgatgagact aagttcgtct ttgatcactt caaggttcag gcaccgcggg ttatcaagac
     5641 ggcagccaac gagacggatc atgtcatgtt ggttgatcac aacgaatttc aacaaagtgt
     5701 cagtgatatc gaagacgtga cgattgattc tgttgtcgac catcatcgca tcgctaactt
     5761 ccacacggca gcgccgctgt tttaccgagc agaaccggtt ggcagcacca ataccgtcat
     5821 ttttgagatg ttccaagaat atggcttcaa gatttcgcca acattggctg gtttgatggc
     5881 ttctggtttg atttcagaca cgctgttgtt gaagtcgccg accgcaaccg acaaggataa
     5941 gaagattttg ccagaaatgg cgaagattgc cggaattgat ttgcagtcat atggtcttgc
     6001 catgctgaag gcaggtacta atttggccgc caagtcagat atggacatta tcgaaggcga
     6061 tgctaagagt ttcaccatgg gcggcaagtc tattcgcatc ggacaggtca ataccgtcga
     6121 tctgaacgac atttacgcac gtcaggacga attgatcagt gccatgacca aagaggcgca
     6181 ggcgaaaggc tacgacgatt tcctgctgat tgccactgac atcttggaca gcaattcaac
     6241 tggtttcgca gtcggtgctg accgcgataa aatcgctagt gctttcaacg aacagttcga
     6301 caaggacgcg cgtttggcat tgcctggggt tgtttcccgc aaaaagcagg tcgttccgcc
     6361 aatgcagagc acgtttgaag gttaactttc atctttaatt gcacacaatt gttctaagga
     6421 aagtcgcagc tagccagttg cggctttttt gtttgg
//
