LOCUS       c00734_gnlPro..         5025 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GPGINDMB_977.
ACCESSION   c00734_gnlPro..
VERSION     c00734_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-29 02:48:31
            Original ID  :: gnl|Prokka|GPGINDMB_977
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5025
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1485
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg734_1"
                     /sec_met_domain="Gly_radical (E-value: 8.2e-43, bitscore:
                     133.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.3e-150, bitscore:
                     490.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="YLAAVKTQNGAAMSVGRIDTVIDAYIQRDMRLGKLNEEQAQELID
                     HLVMKLRMVRFIRTEEYNSLFSGDPIWATLSLAGMGNDGRHHVTKTAFRFLKTLDNMGA
                     APEPNITLLWSERLPEGFKRYATEVSIQSSTIQYENDDLMRNEWGTDYYGIACCVSAQP
                     IADGVQYFGARANLAKTVLYAINGGKDEIQEAQVGPEYAPITSDYIDYKEFMNKFDKMM
                     DWLADTYVNALNVIHYMHDKYYYEAAQLALKDTQLNRTFATGISGLSHAVDSISAIKYG
                     HVKAIRDENGVAVDFVADNDDYPRYGNNDDRADDIAKWLVKTFYNKMNTHHLYRGAKLS
                     TSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPAYGAEKNGALASLMSTAKIPYHYA
                     TDGISNTFGVTPNTLGHDDETRKDTLVHMVDGYMENSGMHLNINVFNKETLIDAQKHPE
                     EYPTLTVRVSGYCVYFADLTKEQQDDVIARTFFEEM"
     protocluster    1..5025
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2468]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2468
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5025
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5025
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             1617..2468
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 65.2;
                     E-value: 2.4e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg734_2"
                     /sec_met_domain="Radical_SAM (E-value: 1e-26, bitscore:
                     82.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLIDQSQSPLNILEQVGQDHDGPIKGYVHSVESFGSVDGPGIRF
                     VVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILADAQRYKAFWGDQGGITCSGGESLV
                     QIDFILELFTKAKALGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAE
                     HKKLTGFPNENILDMVQYMSAHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKNVVQ
                     KVEILPYHTLGVKKYHELGITYPLEGIEPPSADRVKNAENLLHVKDYTGWQSWRPKPVA
                     SN"
     CDS             2593..3561
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     60.8; E-value: 3.2e-19)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg734_3"
                     /sec_met_domain="LysR_substrate (E-value: 6e-28, bitscore:
                     85.7, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 2.1e-20, bitscore: 60.8,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKQERIFSSKTLTYFLQLAETMNYTQAAQLLGITQPALTQQIK
                     KLERTVGAPLFYSVGKKLHLSDAGQTMLKSTHEIYEILNQTTDEIQQTTSATVGKINIG
                     LLSSIEDSAFIAFIAHYYQLNPEVEVTLRMMTRHEIWESLENNQIDLAIMNLPDETIKN
                     WKPYASKKIIDEQLLFLHHDDALANRKRIHFKDTLAEPWATYPDDYFLGQLLREAYKNQ
                     MVDFPHVVAHFTTPYQLTQFVNLSGVNTALPTSFVRARADQIKAYAIAFDPAIRFEISF
                     VYRKEKADIPRISNFFQTFDDYLKEKDYLSRVSENRVKLDK"
     CDS             3591..4523
                     /locus_tag="ctg734_4"
                     /transl_table=11
                     /translation="MTTLIFGHQNPDTDAITSAMSWAEFQKQAGNTDVEAVALGEPNDE
                     TKFVLDHFKVQAPRVIKTAANETDHVMLVDHNEFQQSVSDIEDVTIDSVVDHHRIANFH
                     TAAPLFYRAEPVGSTNTVIFEMFQEYGFKISPTLAGLMASGLISDTLLLKSPTATDKDK
                     KILPEMAKIAGIDLQSYGLAMLKAGTNLAAKSDMDIIEGDAKSFTMGGKSIRIGQVNTV
                     DLNDIYARQDELISAMTKEAQAKGYDDFLLIATDILDSNSTGFAVGADRDKIASAFNEQ
                     FDKDARLALPGVVSRKKQVVPPMQSTFEG"
ORIGIN
        1 tatctggctg ctgttaagac tcaaaacggt gccgcaatgt ccgttggccg gatcgatacg
       61 gttattgatg cttatatcca acgcgatatg cgcttaggca agttgaacga agaacaggca
      121 caggaactga ttgatcattt ggttatgaaa ttgcggatgg ttcgtttcat ccggactgaa
      181 gaatataact cactgttctc aggcgatcca atctgggcaa ccttgtcatt ggcaggaatg
      241 ggcaatgatg gccgtcacca cgttaccaag acggctttcc gtttcctgaa gactttggac
      301 aacatgggcg cagcaccaga accaaacatc accttgcttt ggagcgaacg cttgccagaa
      361 ggattcaaac gctatgcaac cgaagtatcc atccaaagtt caaccattca atatgaaaat
      421 gatgatctga tgcgcaacga atggggtacc gattactacg gtattgcttg ctgtgtctca
      481 gcacaaccaa ttgctgatgg cgttcaatac tttggtgccc gtgctaactt agctaagaca
      541 gttctgtatg cgatcaacgg tgggaaagat gaaattcagg aagcgcaagt agggcctgaa
      601 tatgcaccga tcaccagcga ttacattgat tacaaagaat tcatgaataa gttcgacaag
      661 atgatggact ggctggccga cacgtatgtc aatgctttga atgtgattca ctatatgcat
      721 gacaagtatt attatgaagc tgcacagctg gcattgaagg atacccaact gaaccggacc
      781 ttcgcaaccg ggatttccgg cctgtcacac gcggttgatt ccatcagtgc aatcaagtat
      841 ggtcatgtca aggcaattcg cgatgaaaac ggtgttgcag ttgacttcgt tgctgacaat
      901 gatgactatc cacgttatgg taacaacgat gatcgcgctg atgacatcgc taagtggttg
      961 gttaagacct tctataacaa gatgaacacg catcatctgt atcgcggcgc gaagttgagc
     1021 accagtgtcc tgaccattac ctccaacgtg gtttatggta agaatacagg gacaacacca
     1081 aacggtcgtc aaaaaggcga accattctca ccaggtgcca acccggcata tggcgcagaa
     1141 aagaacggcg cactggcatc cctgatgtcc accgccaaga ttccgtatca ttacgcaact
     1201 gacggcatta gtaatacgtt tggggttact ccgaacacac tgggccacga tgatgaaact
     1261 cgtaaggata ccttggttca catggttgac ggctatatgg aaaacagcgg catgcatttg
     1321 aacatcaacg tcttcaataa ggagactctg attgatgctc agaagcaccc agaagaatac
     1381 ccaacattga ccgttcgggt ttctggctac tgcgtctact tcgcagattt gaccaaggaa
     1441 caacaagacg atgtcattgc tcggaccttc ttcgaagaaa tgtaagcagc ttaactcaag
     1501 acaggaagcc attcctgttt tgccaaaaga cggcaacgat gctgttgccg ttttttggca
     1561 gaatgaaatg aaaatcaatt gaaaacgaca catgatgttg tagtggaggc aataagatga
     1621 aactcatcga tcagtcacaa agcccattaa atatcttaga acaagttggt caggatcatg
     1681 atggcccgat caaagggtat gttcactcgg tcgaaagttt tggttcggtc gatggccctg
     1741 gtattcgctt cgttgtcttt atgcaagggt gtcgcatgcg ttgccagtat tgtcacaacc
     1801 ctgacacctg gaacattggg gttggtgaag aaatgacggc cgatcaaatt ttggcggatg
     1861 cccagcgcta taaagcattc tggggtgacc aagggggtat tacctgcagt ggcggtgaga
     1921 gtttggtaca aatcgatttc attctcgaac tcttcaccaa ggctaaggca ctgggaattt
     1981 cgacttgcct cgatacgtca ggtggcccct tcacgcgtga ccaaccgtgg tttggccagt
     2041 ttgaaaagct gatggctgtt actgacatct cattagttga tattaaacac attgattcgg
     2101 ccgagcataa aaagctcacc ggttttccta atgagaatat tttggatatg gtgcagtata
     2161 tgtcggcgca tggtgatgac atgtggattc gccacgttct ggtcccggaa cgcactgact
     2221 ttgaccctta tctcaaacgg ttaggggact atattgcgac attggacaaa aacgtggtcc
     2281 aaaaagttga gattctgccg tatcacacgt taggcgttaa aaagtatcac gagcttggca
     2341 ttacgtaccc gcttgaaggc atcgaaccgc cgtctgccga tcgcgttaag aatgcagaga
     2401 atctgctgca cgttaaggat tataccggat ggcaaagctg gcgtccgaaa ccagttgcga
     2461 gcaactgaat ttttagctag aacttaacgt aacgattaaa aacgcggcaa attcgttgcg
     2521 ttttttttca ttgccactta tgatgatggt tgaacgatat tattaaatta taaacgaaag
     2581 ggaagccgcc ctatgaaaac gaaacaagag cgcatttttt cctcaaagac actaacttat
     2641 tttctgcaat tggcagaaac catgaactac acccaagccg cccaattgct tggcattacc
     2701 cagccagcct taacacagca gatcaagaaa ttggaacgga cggtgggggc accgctgttt
     2761 tattctgtcg ggaagaaact gcatttatct gacgctggtc agaccatgtt aaagtcaacc
     2821 catgagatct acgagatctt gaaccagaca accgacgaaa ttcaacaaac aaccagtgca
     2881 accgtcggaa aaatcaatat tgggttatta agctcaattg aggatagcgc ctttattgcg
     2941 ttcattgctc attactatca actgaatcct gaagttgaag taacgctacg gatgatgacc
     3001 cgtcacgaaa tttgggaatc attggaaaac aaccagattg atttggctat catgaatctg
     3061 ccagatgaga ccattaagaa ctggaaacca tacgccagca agaaaattat tgatgaacaa
     3121 ttgctgttct tgcaccacga tgacgccttg gctaaccgta agcgaatcca tttcaaagat
     3181 actttagctg aaccttgggc aacttatcct gacgactatt tccttggaca gcttctgcgt
     3241 gaggcgtaca agaatcaaat ggttgatttc ccgcacgtgg tcgcacattt caccacgcca
     3301 tatcagttaa cccaattcgt taacttgtca ggggtcaaca cggccttacc aaccagtttt
     3361 gttcgggcac gcgctgatca aatcaaggcc tatgctattg cgtttgatcc ggccattcgc
     3421 tttgagattt cttttgttta tcgcaaagag aaagctgaca ttccgcggat ctcgaacttc
     3481 ttccagacct ttgacgatta tctgaaggaa aaagattact tgtcgcgggt cagtgaaaac
     3541 cgtgttaaac ttgataagta aactattcag ttgaggagcg atcagataag atgacgactt
     3601 tgatttttgg gcatcagaac ccggacaccg acgcgattac gagtgccatg agctgggcag
     3661 aatttcagaa acaggcaggc aacacggatg ttgaggcggt agcattaggc gaaccgaacg
     3721 atgagactaa gtttgtcctt gatcacttca aggttcaggc accgcgggtt atcaagacgg
     3781 cagccaacga gacggatcat gtcatgttgg ttgatcacaa cgaatttcaa caaagtgtca
     3841 gtgatatcga agacgtgacg attgattctg ttgtcgacca tcatcgcatc gctaacttcc
     3901 acacggcagc gccgctgttt taccgagcag aaccggttgg cagcaccaat accgtcattt
     3961 ttgagatgtt ccaggaatat ggcttcaaga tttcgccaac attggctggt ttgatggctt
     4021 ctggtttgat ttcagacacg ctgttgttga agtcgccgac cgcaaccgac aaggataaga
     4081 agattttgcc agaaatggcg aagattgccg gaattgattt gcagtcatat ggtcttgcca
     4141 tgctgaaggc aggcactaat ttggccgcca agtcagatat ggacattatc gaaggcgatg
     4201 ctaagagttt caccatgggc ggcaagtcta ttcgcatcgg acaggtcaat accgtcgatc
     4261 tgaacgacat ttacgcacgt caggacgaat tgatcagtgc catgaccaaa gaggcgcagg
     4321 cgaaaggcta cgacgatttc ctgctgattg ccactgacat cttggacagc aattcaactg
     4381 gtttcgcagt cggtgctgac cgcgataaaa tcgccagtgc tttcaacgaa cagttcgaca
     4441 aggacgcgcg tttggcattg cctggggttg tttcccgcaa aaagcaggtc gttccgccaa
     4501 tgcagagcac gtttgaaggt taactttcat ctttaattgc acacaattgt tctaaggtaa
     4561 gtcgcagcta gccagttgcg gcttttttgt ttgcaaaaag tctctagcca tctttttgcg
     4621 aaatctgtta aatataaagc tggctagcag ttcgtgatgc gtgaatttga aatttgtgta
     4681 gcaattcgcg ggtcgaatca acagcaagtt gtcatgggtc attttggatg gttttgaggc
     4741 atgctgagag acgcattctg agacgagttc gggaattcaa cttgatagtt tgacttaacg
     4801 aaaaaactca agcaaaaaat atccaatgat ttatccaaag attgtagaag cttttaaaga
     4861 cttggctgca agcagcggtt caaaaaatga ggcgactatc aggtgctgta tgaaattgcg
     4921 aatcaaataa tcgccaacgt aaatattgcg agaatggcac gatgccgtct cttcaaacgc
     4981 tacttcgcat aatatatatt atgtaaagta gaatataaat aattg
//
