LOCUS       c00379_gnlPro..         3757 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PBCGDJGH_514.
ACCESSION   c00379_gnlPro..
VERSION     c00379_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-30 02:58:17
            Original ID  :: gnl|Prokka|PBCGDJGH_514
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3757
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3757
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:2301]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..2301
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3757
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3757
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..1318
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg379_1"
                     /sec_met_domain="Gly_radical (E-value: 6.6e-43, bitscore:
                     133.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 5.9e-124, bitscore:
                     402.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="IRTEEYNSLFSGDPIWATLSLAGMGNDGRHHVTKTAFRFLKTLDN
                     MGAAPEPNITLLWSERLPEGFKRYATEVSIQSSTIQYENDDLMRNEWGTDYYGIACCVS
                     AQPIADGVQYFGARANLAKTVLYAINGGKDEIQEAQVGPEYAPITSDYIDYKEFMNKFD
                     KMMDWLADTYVNALNVIHYMHDKYYYEAAQLALKDTQLNRTFATGISGLSHAVDSISAI
                     KYGHVKAIRDENGVAVDFVADNDDYPRYGNNDDRADDIAKWLVKTFYNKMNTHHLYRGA
                     KLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPAYGAEKNGALASLMSTAKIPY
                     HYATDGISNTFGVTPNTLGHDDETRKDTLVHMVDGYMENSGMHLNINVFNKETLIDAQK
                     HPEEYPTLTVRVSGYCVYFADLTKEQQDDVIARTFFEEM"
     CDS             1450..2301
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 65.2;
                     E-value: 2.4e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg379_2"
                     /sec_met_domain="Radical_SAM (E-value: 1e-26, bitscore:
                     82.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLIDQSQSPLNILEQVGQDHDGPIKGYVHSVESFGSVDGPGIRF
                     VVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILADAQRYKAFWGDQGGITCSGGESLV
                     QIDFILELFTKAKALGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAE
                     HKKLTGFPNENILDMVQYMSAHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKNVVQ
                     KVEILPYHTLGVKKYHELGITYPLEGIEPPSADRVKNAENLLHVKDYTGWQSWRPKPVA
                     SN"
     CDS             2426..3394
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     60.8; E-value: 3.2e-19)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg379_3"
                     /sec_met_domain="LysR_substrate (E-value: 6e-28, bitscore:
                     85.7, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 2.1e-20, bitscore: 60.8,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKQERIFSSKTLTYFLQLAETMNYTQAAQLLGITQPALTQQIK
                     KLERTVGAPLFYSVGKKLHLSDAGQTMLKSTHEIYEILNQTTDEIQQTTSATVGKINIG
                     LLSSIEDSAFIAFIAHYYQLNPEVEVTLRMMTRHEIWESLENNQIDLAIMNLPDETIKN
                     WKPYASKKIIDEQLLFLHHDDALANRKRIHFKDTLAEPWATYPDDYFLGQLLREAYKNQ
                     MVDFPHVVAHFTTPYQLTQFVNLSGVNTALPTSFVRARADQIKAYAIAFDPAIRFEISF
                     VYRKEKADIPRISNFFQTFDDYLKEKDYLSRVSENRVKLDK"
     CDS             3424..3756
                     /locus_tag="ctg379_4"
                     /transl_table=11
                     /translation="MTTLIFGHQNPDTDAITSAMSWAEFQKQAGNTDVEAVALGEPNDE
                     TKFVLDHFKVQAPRVIKTAANETDHVMLVDHNEFQQSVSDIEDVTIDSVVDHHRIANFH
                     TAAPLFT"
ORIGIN
        1 catccggact gaagaatata actcactgtt ctcaggcgat ccaatctggg caaccttgtc
       61 attggcagga atgggcaatg atggccgtca ccacgttacc aagacggctt tccgtttcct
      121 gaagactttg gacaacatgg gcgcagcacc agaaccaaac atcaccttgc tttggagcga
      181 acgcttgcca gaaggcttca aacgctatgc aaccgaagta tccatccaaa gttcaaccat
      241 tcaatatgaa aatgatgacc tgatgcgcaa cgaatggggt accgattact acggtattgc
      301 ttgctgtgtc tcggcacaac caattgctga tggcgttcaa tactttggtg cccgtgctaa
      361 cttagctaag acagttctgt atgcgatcaa cggtgggaaa gatgaaattc aggaagcgca
      421 agtagggcct gaatatgcac cgatcaccag cgattacatt gattacaaag aattcatgaa
      481 taagttcgac aagatgatgg actggctggc cgacacgtat gtcaatgctt tgaatgtgat
      541 tcactatatg catgacaagt attattatga agctgcacag ctggcattga aggataccca
      601 actgaaccgg accttcgcaa ccgggatttc cggcctgtca cacgcagttg attccatcag
      661 tgcaatcaag tatggtcatg tcaaggcaat tcgcgatgaa aacggtgttg cagttgactt
      721 cgttgctgac aatgatgact atccacgcta tggtaacaac gatgatcgcg ctgatgacat
      781 cgctaagtgg ttggttaaga ccttctataa caagatgaac acgcatcatc tgtatcgcgg
      841 cgcgaagttg agtaccagtg tcctgaccat tacctccaac gtggtttatg gtaagaatac
      901 agggacaaca ccaaacggtc gtcaaaaagg cgaaccattc tcaccaggtg ccaacccggc
      961 atacggcgca gaaaagaacg gcgcattggc atccttgatg tccaccgcca agattccgta
     1021 tcattacgca actgacggca ttagtaatac gtttggggtt actccgaaca cactgggcca
     1081 cgatgatgaa actcgtaagg ataccttggt tcacatggtt gacggctata tggaaaacag
     1141 cggcatgcat ttgaacatca acgtcttcaa caaggagacc ctgattgatg ctcagaagca
     1201 cccagaagaa tacccaacat tgaccgttcg ggtttctggc tactgcgtct acttcgcaga
     1261 tttgaccaag gaacaacaag acgatgtcat tgctcggacc ttcttcgaag aaatgtaagc
     1321 agcttaactc aagacaggaa gccattcctg ttttgccaaa agacggcaac gatgctgttg
     1381 ccgttttttg gcagaatgaa atgaaaatca attgaaaacg acacatgatg ttgtagtgga
     1441 ggcaataaga tgaaactcat cgatcagtca caaagcccat taaatatctt agaacaagtt
     1501 ggtcaggatc atgatggccc gatcaaaggg tatgttcact cggtcgaaag ttttggttcg
     1561 gtcgatggcc ctggtattcg cttcgttgtc tttatgcaag ggtgtcgcat gcgttgccag
     1621 tattgtcaca accctgacac ctggaacatt ggggttggtg aagaaatgac ggccgatcaa
     1681 attttggcgg atgcccagcg ctataaagca ttctggggtg accaaggggg tattacctgc
     1741 agtggcggtg agagcttggt acaaatcgat ttcattctcg aactcttcac caaggctaag
     1801 gcactgggaa tttcgacttg cctcgatacg tcaggcggcc ccttcacgcg tgaccaaccg
     1861 tggtttggcc agtttgaaaa gctgatggct gttactgaca tctcattagt tgatattaaa
     1921 cacattgatt cggccgagca taaaaagctc accggttttc ctaatgaaaa tattttggat
     1981 atggtgcagt atatgtcggc gcatggtgat gacatgtgga ttcgccacgt tctggtcccg
     2041 gaacgcactg actttgaccc ttatctcaaa cggttagggg actatattgc gacattggac
     2101 aaaaatgtgg tccaaaaagt tgagattctg ccgtatcaca cattaggcgt taaaaagtat
     2161 cacgagcttg gcattacgta cccgcttgaa ggcatcgaac cgccgtctgc cgatcgcgtt
     2221 aagaatgcag agaatctgct gcacgttaag gattataccg gatggcaaag ctggcgtccg
     2281 aaaccagttg cgagcaactg aatttttagc tagaacttaa cgtaacgatt aaaaacgcgg
     2341 caaattcgtt gcgttttttt tcattgccac ttatgatgat ggttgaacga tattattaaa
     2401 ttataaacga aagggaagcc gccctatgaa aacgaaacaa gagcgcattt tttcctcaaa
     2461 gacactaact tattttctgc aattggcaga aaccatgaac tacacccaag ccgcccaatt
     2521 gcttggcatt acccagccag ccttaacaca gcagatcaag aaattggaac ggacggtggg
     2581 ggcaccgctg ttttattctg ttgggaagaa actgcattta tctgacgctg gtcagaccat
     2641 gttaaagtca acccatgaga tctacgagat cttgaaccag acaaccgacg aaattcaaca
     2701 aacaaccagt gcaaccgtcg gaaaaatcaa tattgggtta ttaagctcaa tcgaggatag
     2761 cgcctttatt gcgttcattg ctcattacta tcaattgaat cctgaagttg aagtgacgct
     2821 acggatgatg acccgtcacg aaatttggga atccttggaa aacaaccaga ttgatttggc
     2881 tatcatgaat ctgccagatg aaaccattaa gaactggaaa ccatacgcca gcaagaaaat
     2941 tattgatgaa caattgctgt tcttgcacca cgatgacgcc ttggctaacc gtaagcgaat
     3001 ccatttcaaa gatactttag ctgaaccttg ggcaacttat cctgacgact atttccttgg
     3061 acagcttctg cgtgaggcgt acaagaatca aatggttgat ttcccgcacg tggtcgcaca
     3121 tttcaccacg ccatatcagt taacccaatt cgttaacttg tcaggggtca acacggcctt
     3181 accaaccagt tttgttcggg cacgcgctga tcaaatcaag gcctatgcta ttgcgtttga
     3241 tccggccatt cgctttgaga tttcttttgt ttatcgcaaa gagaaagctg acattccgcg
     3301 gatctcgaac ttcttccaga cctttgacga ttatctgaag gaaaaagatt acttgtcgcg
     3361 ggtcagtgaa aaccgtgtta aacttgataa gtaaactatt cagttgagga gcgatcagat
     3421 aagatgacga ctttgatttt tgggcatcag aacccggaca ccgacgcgat tacgagtgcc
     3481 atgagctggg cagaatttca gaaacaggca ggcaacacgg atgttgaggc ggtagcatta
     3541 ggcgaaccga acgatgaaac taagttcgtc cttgatcact tcaaggttca ggcaccgcgg
     3601 gttatcaaga cggcagccaa cgagacggat catgtcatgt tggttgatca caacgaattt
     3661 caacaaagtg tcagtgatat cgaagacgtg acgattgatt ctgttgtcga ccatcatcgc
     3721 atcgctaact tccacacggc agcgccgctg tttaccg
//
