LOCUS       c00438_gnlPro..         3072 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DDOLEHNA_519.
ACCESSION   c00438_gnlPro..
VERSION     c00438_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-29 03:06:12
            Original ID  :: gnl|Prokka|DDOLEHNA_519
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3072
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3072
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[27:3071]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      28..3071
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3072
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3072
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(28..879)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 65.2;
                     E-value: 2.4e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg438_1"
                     /sec_met_domain="Radical_SAM (E-value: 5.2e-27, bitscore:
                     82.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLIDQSQSPLNILEQVGQDHDGPIKGYVHSVESFGSVDGPGIRF
                     VVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILADAQRYKAFWGDQGGITCSGGESLV
                     QIDFILELFTKAKALGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAE
                     HKKLTGFPNENILDMVQYMSAHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKNVVQ
                     KVEILPYHTLGVKKYHELGITYPLEGIEPPSADRVKNAENLLHVKDYTGWQSWRPKPVA
                     SN"
     CDS             complement(1011..3071)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg438_2"
                     /sec_met_domain="Gly_radical (E-value: 6.9e-43, bitscore:
                     132.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2e-233, bitscore:
                     763.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="ANGGVLDADNNIPSTITSHGPGYLNKDLEKIVGVQTDKPFKRAFM
                     PFGGIRMAEDALESYGFKTDPQEHKIFNEYRKTHNQGVFDAYTPDMRKARHYKIVTGLP
                     DAYGRGRIVSDFPRIAVYGIDRLMAEKFKDYNLTGDGEMTDDVIKLREEINEQYRALND
                     MKKMAKEYGYDISRPAANAQEAVQWIYFGYLAAVKTQNGAAMSVGRIDTVIDAYIQRDM
                     RLGKLNEEQAQELIDHLVMKLRMVRFIRTEEYNSLFSGDPIWATLSLAGMGNDGRHHVT
                     KTAFRFLKTLDNMGAAPEPNITLLWSERLPEGFKRYATEVSIQSSTIQYENDDLMRNEW
                     GTDYYGIACCVSAQPIADGVQYFGARANLAKTVLYAINGGKDEIQEAQVGPEYAPITSD
                     YIDYKEFMNKFDKMMDWLADTYVNALNVIHYMHDKYYYEAAQLALKDTQLNRTFATGIS
                     GLSHAVDSISAIKYGHVKAIRDENGVAVDFVADNDDYPRYGNNDDRADDIAKWLVKTFY
                     NKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPAYGAEKNGA
                     LASLMSTAKIPYHYATDGISNTFGVTPNTLGHDDETRKDTLVHMVDGYMENSGMHLNIN
                     VFNKETLIDAQKHPEEYPTLTVRVSGYCVYFADLTKEQQDDVIARTFFEEM"
ORIGIN
        1 aatcgttacg ttaagtctag ctaaaattca gttgctcgca actggtttcg gacgccagct
       61 ttgccatccg gtataatcct taacgtgcag cagattctct gcattcttaa cgcgatcggc
      121 agacggcggt tcgatgcctt caagcgggta cgtaatgcca agctcgtgat actttttaac
      181 gcctaacgtg tgatacggca gaatctcaac tttttggacc acgtttttgt ccaatgtcgc
      241 aatatagtcc cctaaccgtt tgagataagg gtcaaagtca gtgcgttccg ggaccagaac
      301 gtggcgaatc cacatgtcat caccatgcgc cgacatatac tgcaccatat ccaaaatatt
      361 ttcattagga aaaccggtga gctttttatg ctcggccgaa tcaatgtgtt ttatatcaac
      421 taatgagatg tcagtaacag ccatcagctt ttcaaactgg ccaaaccacg gttggtcacg
      481 cgtgaagggg ccgcctgacg tatcgaggca agtcgaaatt cccagtgcct tagccttggt
      541 gaagagttcg agaatgaaat cgatttgtac caaactctca ccgccactgc aggtaatacc
      601 cccttggtca ccccagaaag ctttatagcg ctgggcatcc gccaaaattt gatcagccgt
      661 catttcttca ccaaccccaa tgttccaggt gtcagggttg tgacaatact ggcaacgcat
      721 gcgacaccct tgcataaaga caacgaagcg aataccaggg ccatcgaccg aaccaaaact
      781 ttcgaccgag tgaacatacc ctttgatcgg gccatcatga tcctgaccaa cttgttctaa
      841 gatatttaat gggctttgtg actgatcgat gagtttcatc ttattgcctc cactacaaca
      901 tcatgtgtcg ttttcaattg attttcattt cattctgcca aaaaacggca acagcatcgt
      961 tgccgtcttt tggcaaaaca ggaatgactt cctgtcttga gttaagctgc ttacatttct
     1021 tcgaagaagg tccgagcaat gacatcgtct tgttgttcct tggtcaaatc tgcgaagtag
     1081 acgcagtagc cagaaacccg aacggtcaat gttgggtatt cttctgggtg cttctgagca
     1141 tcaatcaggg tctccttgtt gaagacgttg atgttcaaat gcatgccgct gttttccata
     1201 tagccgtcaa ccatgtgaac caaggtatcc ttacgagttt catcatcgtg gcccagtgtg
     1261 ttcggagtaa ccccaaacgt attactaatg ccgtcagttg cgtaatgata cggaatcttg
     1321 gcggtggaca tcagggatgc caatgcgccg ttcttttctg cgccgtatgc cgggttggca
     1381 cctggtgaga atggttcgcc tttttgacga ccgtttggtg ttgtccctgt attcttacca
     1441 taaaccacgt tggaagtaat ggtcaggaca ctggtactca acttcgcgcc gcgatacaga
     1501 tgatgcgtgt tcatcttgtt atagaaggtc ttaaccaacc acttagcgat gtcatcagcg
     1561 cgatcatcgt tgttaccata gcgtggatag tcatcattgt cagcaacgaa gtcaactgca
     1621 acaccgtttt catcgcgaat tgccttgaca tgaccatact tgattgcact gatggaatca
     1681 actgcgtgtg acaggccgga aatcccggtt gcgaaggtcc ggttcagttg ggtatccttc
     1741 aatgccagct gtgcagcttc ataataatac ttgtcatgca tatagtgaat cacattcaaa
     1801 gcattgacat acgtgtcggc cagccagtcc atcatcttgt cgaacttatt catgaattct
     1861 ttgtaatcaa tgtaatcgct ggtgatcggt gcatattcag gccctacttg cgcttcctga
     1921 atttcatctt tcccaccgtt gatcgcatac agaactgtct tagctaagtt agcacgggca
     1981 ccaaagtatt gaacgccatc agcaattggt tgtgccgaga cacagcaagc aataccgtag
     2041 taatcggtac cccattcgtt gcgcatcaga tcatcatttt catattgaat ggttgaactt
     2101 tggatggata cttcggttgc atagcgtttg aagccttctg gcaagcgttc gctccaaagc
     2161 aaggtgatgt ttggttctgg tgctgcgccc atgttgtcca aagtcttcag gaaacggaaa
     2221 gccgtcttgg taacgtggtg acggccatca ttgcccattc ctgccaatga caaggttgcc
     2281 cagattggat cgcctgagaa cagtgagtta tattcttcag tccggatgaa acgaaccatc
     2341 cgcaatttca taaccaaatg atcaatcagt tcctgtgcct gttcttcgtt caatttgcct
     2401 aagcgcatat cgcgttggat ataagcatca ataaccgtat cgatccggcc aacggacatt
     2461 gcggcaccgt tttgagtctt aacagcagcc agatagccaa agtagatcca ctgaacagct
     2521 tcttgcgcat tagctgccgg acgcgagatg tcatagccat attccttggc catcttcttc
     2581 atgtcgttta aggcacgata ttgttcattg atttcttcac gcagtttgat cacatcgtca
     2641 gtcatttcgc cgtcgccagt caagttataa tccttgaact tctcggccat caaacgatcg
     2701 atcccgtaaa cagcgatccg tgggaaatca gaaacaatcc ggccacgacc atatgcgtct
     2761 ggcaagccag tcacgatctt gtaatggcga gcctttcgca tatcaggggt ataagcatca
     2821 aagacacctt ggttatgggt cttacgatat tcattgaaaa tcttgtgttc ttgaggatct
     2881 gtcttgaagc cataagattc caatgcatct tcagccattc gaataccgcc aaatggcatg
     2941 aatgctcgtt tgaatggctt gtcggtctga acaccaacaa tcttttcaag atctttgttc
     3001 aaataaccag ggccgtgtga ggtgatcgta gatggaatat tgttgtcagc atccaaaaca
     3061 ccgccatttg ca
//
