LOCUS       c00215_gnlPro..         4218 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PNFIDCEI_215.
ACCESSION   c00215_gnlPro..
VERSION     c00215_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-30 02:48:42
            Original ID  :: gnl|Prokka|PNFIDCEI_215
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4218
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4218
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[42:3287]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      43..3287
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4218
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4218
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(43..894)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 65.2;
                     E-value: 2.4e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg215_1"
                     /sec_met_domain="Radical_SAM (E-value: 1e-26, bitscore:
                     82.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLIDQSQSPLNILEQVGQDHDGPIKGYVHSVESFGSVDGPGIRF
                     VVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILADAQRYKAFWGDQGGITCSGGESLV
                     QIDFILELFTKAKALGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAE
                     HKKLTGFPNENILDMVQYMSAHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKNVVQ
                     KVEILPYHTLGVKKYHELGITYPLEGIEPPSADRVKNAENLLHVKDYTGWQSWRPKPVA
                     SN"
     CDS             complement(1026..3287)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg215_2"
                     /sec_met_domain="Gly_radical (E-value: 1.6e-42, bitscore:
                     132.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 6.8e-245, bitscore:
                     802.0, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQLDETKVPNYWEGFNGGDWQEEINVRDFIEHNLNQYDGDESFL
                     AGPTEATTVLNNQVLNLKKQERANGGVLDADNNIPSTITSHGPGYLNKDLEKIVGVQTD
                     KPFKRAFMPFGGIRMAEDALESYGFKTDPQEHKIFNEYRKTHNQGVFDAYTPDMRKARH
                     YKIVTGLPDAYGRGRIVSDFPRIAVYGIDRLMAEKFKDYNLTGDGEMTDDVIKLREEIN
                     EQYRALNDMKKMAKEYGYDISRPAANAQEAVQWIYFGYLAAVKTQNGAAMSVGRIDTVI
                     DAYIQRDMRLGKLNEEQAQELIDHLVMKLRMVRFIRTEEYNSLFSGDPIWATLSLAGMG
                     NDGRHHVTKTAFRFLKTLDNMGAAPEPNITLLWSERLPEGFKRYATEVSIQSSTIQYEN
                     DDLMRNEWGTDYYGIACCVSAQPIADGVQYFGARANLAKTVLYAINGGKDEIQEAQVGP
                     EYAPITSDYIDYKEFMNKFDKMMDWLADTYVNALNVIHYMHDKYYYEAAQLALKDTQLN
                     RTFATGISGLSHAVDSISAIKYGHVKAIRDENGVAVDFVADNDDYPRYGNNDDRADDIA
                     KWLVKTFYNKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPA
                     YGAEKNGALASLMSTAKIPYHYATDGISNTFGVTPNTLGHDDETRKDTLVHMVDGYMEN
                     SGMHLNINVFNKETLIDAQKHPEEYPTLTVRVSGYCVYFADLTKEQQDDVIARTFFEEM
                     "
     CDS             complement(3539..4084)
                     /locus_tag="ctg215_3"
                     /transl_table=11
                     /translation="MAMKLKDPAAIVTNAYFTTDQQQDVFVVSRHAYGLTFPLSDVSTV
                     GARATGVKSMDLKPDDEVVNFILVKDPTTAKVGILTQRGAFKKMALSEVGEMSRARRGL
                     LVLRELKRDPHRIVAMMSVDDATRLNVLTDTGKVTTLNPAQHPAGDRYSNGSFVIDTDV
                     LGKPVFVQTKEEPADQVE"
     CDS             complement(4126..4218)
                     /locus_tag="ctg215_4"
                     /transl_table=11
                     /translation="VLAVFAFDQLDGAGSFVLGSSDGYXKPKLP"
ORIGIN
        1 gccgccgttt ttaatcgtta cgttaagttc taagctaaaa attcagttgc tcgcaactgg
       61 tttcggacgc cagctttgcc atccggtata atccttaacg tgcagcagat tctctgcatt
      121 cttaacgcga tcggcagacg gcggttcgat gccttcaagc gggtacgtaa tgccaagctc
      181 gtgatacttt ttaacgccta acgtgtgata cggcagaatc tcaacttttt ggaccacgtt
      241 tttgtccaat gtcgcaatat agtcccctaa ccgtttgaga taagggtcaa agtcagtgcg
      301 ttccgggacc agaacgtggc gaatccacat gtcatcacca tgcgccgaca tatactgcac
      361 catatccaaa atattttcat taggaaaacc ggtgagcttt ttatgctcgg ccgaatcaat
      421 gtgtttaata tcaactaatg agatgtcagt aacagccatc agcttttcaa actggccaaa
      481 ccacggttgg tcacgcgtga aggggccgcc tgacgtatcg aggcaagtcg aaattcccag
      541 tgccttagcc ttggtgaaga gttcgagaat gaaatcgatt tgtaccaaac tctcaccgcc
      601 actgcaggta ataccacctt ggtcacccca gaatgcttta tagcgctggg catccgccaa
      661 aatttgatcg gccgtcattt cttcaccaac cccaatgttc caggtgtcag ggttgtgaca
      721 atactggcaa cgcatgcgac acccttgcat aaagacaacg aagcgaatac cagggccatc
      781 gaccgaacca aaactttcga ccgagtgaac ataccctttg atcgggccat catgatcctg
      841 accaacttgt tctaagatat ttaatgggct ttgtgactga tcgatgagtt tcatcttatt
      901 gcctccacta caacatcatg tgtcgttttc aattgatttt catttcattc tgccaaaaaa
      961 cggcaacagc atcgttgccg tcttttggca aaacaggaat ggcttcctgt cttgagttaa
     1021 gctgcttaca tttcttcgaa gaaggtccga gcaatgacat cgtcttgttg ttccttggtc
     1081 aaatctgcga agtagacgca gtagccagaa acccgaacgg tcaatgttgg gtattcttct
     1141 gggtgcttct gagcatcaat cagggtctcc ttgttaaaga cgttgatgtt caaatgcatg
     1201 ccgctgtttt ccatatagcc gtcaaccatg tgaaccaagg tatccttacg agtttcatca
     1261 tcgtggccca gtgtgttcgg agtaacccca aacgtattac taatgccgtc agttgcgtaa
     1321 tgatacggaa tcttggcggt ggacatcagg gatgccaatg cgccgttctt ttctgcgccg
     1381 tatgccgggt tggcacctgg tgagaatggt tcgccttttt gacgaccgtt tggtgttgtc
     1441 cctgtattct taccataaac cacgttggaa gtaatggtca ggacactggt actcaacttc
     1501 gcgccgcgat acagatgatg cgtgttcatc ttgttataga aggtcttaac caaccactta
     1561 gcgatgtcat cagcgcgatc atcgttgtta ccatagcgtg gatagtcatc attgtcagca
     1621 acgaagtcaa ctgcaacacc gttttcatcg cgaattgcct tgacatgacc atacttgatt
     1681 gcactgatgg aatcaactgc gtgtgacagg ccggaaatcc cggttgcgaa ggtccggttc
     1741 agttgggtat ccttcaatgc cagctgtgca gcttcataat aatacttgtc atgcatatag
     1801 tgaatcacat tcaaagcatt gacatacgtg tcggccagcc agtccatcat cttgtcgaac
     1861 ttattcatga attctttgta atcaatgtaa tcgctggtga tcggtgcata ttcaggccct
     1921 acttgcgctt cctgaatttc atctttccca ccgttgatcg catacagaac tgtcttagct
     1981 aagttagcac gggcaccaaa gtattgaacg ccatcagcaa ttggttgtgc cgagacacag
     2041 caagcaatac cgtagtaatc ggtaccccat tcgttgcgca tcaggtcatc attttcatat
     2101 tgaatggttg aactttggat ggatacttcg gttgcatagc gtttgaagcc ttctggcaag
     2161 cgttcgctcc aaagcaaggt gatgtttggt tctggtgctg cgcccatgtt gtccaaagtc
     2221 ttcaggaaac ggaaagccgt cttggtaacg tggtgacggc catcattgcc cattcctgcc
     2281 aatgacaagg ttgcccagat tggatcgcct gagaacagtg agttatattc ttcagtccgg
     2341 atgaaacgaa ccatccgcaa tttcataacc aaatgatcaa tcagttcctg tgcctgttct
     2401 tcgttcaatt tgcctaagcg catatcgcgt tggatataag catcaataac cgtatcgatc
     2461 cggccaacgg acattgcggc accgttttga gtcttaacag cagccagata gccaaagtag
     2521 atccactgaa cagcttcttg cgcattagct gccggacgcg agatgtcata gccatattcc
     2581 ttggccatct tcttcatgtc gtttaaggca cgatattgtt cattgatttc ttcacgcagt
     2641 ttgattacat cgtcagtcat ttcgccgtcg ccggtcaagt tataatcctt gaacttctca
     2701 gccattaaac gatcgatccc gtaaacagcg atccgtggga aatcagaaac aatccggcca
     2761 cggccgtatg cgtctggcaa gccagtcacg atcttgtaat ggcgagcctt tcgcatatca
     2821 ggggtataag catcaaagac accttggtta tgggtcttac gatattcatt gaaaatcttg
     2881 tgttcttgag gatctgtctt gaagccataa gattccaatg catcttcagc cattcgaata
     2941 ccgccaaatg gcatgaatgc tcgtttgaat ggcttgtcgg tctgaacacc aacaatcttt
     3001 tcaagatctt tgttcaaata accaggtccg tgtgaggtga tcgtagatgg aatattgttg
     3061 tcagcatcca aaacaccgcc atttgcacgt tcttgcttct ttaaatttag aacctgattg
     3121 ttcaggactg ttgtcgcttc agtggggcca gcaaggaaac tttcgtcccc gtcatattgg
     3181 ttcaggttat gctcgataaa atcgcgcaca ttaatttctt cttgccagtc gccgccgtta
     3241 aagccttccc aataattagg aactttggtt tcgtcaagct gtttcatagt tgtttgcctc
     3301 ctaaagtgga ttacagatgt gacagttctt acaaacacag tataacacgc cgccaaatct
     3361 atgcaagcgt tttattgccc atataatagg cgttatattg ttttttaagt gtgcttcgtc
     3421 gcttcttttc gcccatgatt tcgtcatttt gttaaattgt gcacaactga tctgtcctat
     3481 aataatcaaa aacgccgcca tctgtattag tacagacggc ggcgtcaatg atcaccattt
     3541 attcgacttg atctgctggt tcttctttgg tttgaacaaa aactggcttg ccaaggacat
     3601 cagtgtcaat gacaaacgaa ccattcgaat agcggtcacc tgctggatgt tgtgccggat
     3661 taagggtcgt taccttaccc gtatcggtca aaacattcag gcgggtagcg tcatcgactg
     3721 acatcatcgc gacaatccga tgcggatcac gcttaagctc acgcaagacc agtaaaccac
     3781 gacgggcacg actcatttcc ccgacttcgc ttaaggccat ctttttgaag gcgccacgtt
     3841 gggtcaaaat accgacctta gccgtagttg gatccttcac tagaatgaaa ttgacaacct
     3901 catcgtctgg ctttagatcc atacttttaa cgccggttgc ccgggcaccg acagttgaca
     3961 catcgcttaa cgggaatgtc agtccgtaag cgtggcggct gaccacaaag acatcctgtt
     4021 gctgatcagt cgtgaagtat gcattggtga caatcgcggc tgggtctttc agcttcatcg
     4081 ccatcatcgg cttactcttg tacgtccgtt gcggctgcaa atcacttaag gcagtttggg
     4141 tttnatgtac ccgtcacttg accctaacac aaaactgcca gcgccatcga gttgatcaaa
     4201 agcaaacact gccaagac
//
