LOCUS       c00177_gnlPro..         3871 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NAMJHKCF_195.
ACCESSION   c00177_gnlPro..
VERSION     c00177_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-03 16:55:39
            Original ID  :: gnl|Prokka|NAMJHKCF_195
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3871
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3871
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[646:3716]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      647..3716
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3871
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3871
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             647..2977
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg177_1"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-42, bitscore:
                     132.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.9e-250, bitscore:
                     819.4, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQKEQWQGFNGRIWREEINLREFIQDNYTPYDGDASFLEGPTEA
                     TDTLWGELQKLQKAEREKGGVLDMDTDIVSSLTSHKPGYISESLKEKEQIVGLQTDKPL
                     KRAFMPFGGIRTAEEACTTYGYTPNPEFHKIFTDYHKTHNQAVFDSYTPEMKKARHNKI
                     ITGLPDTYGRGRIVGXXXXXXXXXXXXXXXXRVALYGIDLLVADKQKDFANCGDGTMTD
                     DVIRLREEISMQIKALKDMKVMAESYGYDISEPASNAKEAVQWTYFGYLAAIKTQNGAA
                     MSIGRIATFLDIYIKRDMDKGILTEKEAQELIDHMTMKFRMVKFGRIPAYNQLFSGDPV
                     WATLEVGGLGQDGRSMVTKTDYRFLHTLENMGPSPEPNLTVLYSERLPKAFRDYAAHIS
                     ITTSSIQYENDDVMRPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVD
                     EKTGQQVGPEYKPITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYEYAL
                     MALIDTNVRRTFATGIAGFSHVVDSLSAXXXXXXXXXXXXXXXXXXVKTVRNEEGLVVD
                     YETTGDFPKYGNDDDRADDIAVWLLQTFMKKLEKFHTYRDSEPTTSILTITSNVVYGKA
                     TGSLPDGRKAGEPLSPGANPSYGAEQNGLLASLNSVAKLPYEWALDGISNTQTINPDAL
                     GHSEEERIGNLVNVMDGYFAQGAHHLNVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYA
                     VKFISLTKEQQLDVIARTCHDRM"
     CDS             2964..3716
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 76.2;
                     E-value: 1e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg177_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.3e-33, bitscore:
                     103.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTECNKNAMALVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNP
                     DTWKMTEENGAVWRSADELLNQALRYRTYWKNSGGITVSGGEPLLQIDFMLEFFKKAKE
                     KGIHTVIDTAGGPFTREEPFFSKFQELMKYTDLLLVDIKHIDNKYHKVLTGRSNENILD
                     MIRYLSDIKKPIWIRHVLVPERSDKDEYLTRLADFIHSLDNVEKVEILPYHTMGIYKWK
                     ELGLEYPLEGIDPPTKERVENAKKILAI"
ORIGIN
        1 cacaaatatg ccttattggg ggcaatggct tcgtctgtag ttctcttaat gaattaaata
       61 gtaacccctt gacattttgt tctattttgt ttatagttag gatacttata cgactgacgg
      121 aagtaagtgg aatgaaccac atgaagtata agactcttat agccgaccgt ctgggcaatt
      181 cttgtctgga tgctggcttt tttatttctg ataagataat attctatcca atatccctga
      241 taagaagtaa ctttaaatcg ttattaagtc atgtgctaaa gaagatgttt ttccaaacat
      301 gctctaaaga gcgaaaaata aaattatagt tgtatgcttg tcattattgt acttatatca
      361 gtacatttgt acgatttgat aatattttat caacgctcta aaacccttta ttttaaaggc
      421 tctacaagca tttttgtgta tatgttcaaa aaaaagaaca cataatatca aaaaattgtt
      481 tgacgattgc caaaaatcat gttaataatg ttgttagatg attaaaactt aaataagtta
      541 aaactaatca tagtaaatca tctgaacaat tttgttgatt ttttagcaaa catatttcaa
      601 aaccttgact taaggatttt tttaacaaac agggaggtca attaagatgt tacaaaaaga
      661 acaatggcag ggatttaatg gacgaatctg gagagaagaa attaatttaa gagagttcat
      721 ccaggataac tacactccat atgatggaga tgcttctttc ctcgaaggtc ctaccgaagc
      781 tactgacaca ctgtggggcg aactgcagaa gctgcagaaa gcagagcgtg aaaagggtgg
      841 tgttcttgat atggatactg atatcgtatc aagccttact tcccataaac cgggttacat
      901 ttctgagtct ttaaaagaga aagaacagat cgtaggtctt cagacagata aacctttaaa
      961 acgtgcattt atgccatttg gtggtatccg tactgctgaa gaagcttgta caacttatgg
     1021 ttacacacca aatccagaat tccacaagat ttttacagat tatcataaga cacataacca
     1081 ggcagtattt gattcttata ctcctgaaat gaagaaagct cgtcacaata agatcattac
     1141 tggtcttcca gatacttacg gacgtggacg tatcgttggt gnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnngtcg tgtagctctt tacggtattg acctgttagt
     1261 tgctgacaag caaaaagatt tcgcaaactg cggtgacgga acaatgacag atgacgttat
     1321 ccgtttaaga gaagaaatct ccatgcagat taaagcatta aaagatatga aagttatggc
     1381 tgaaagctat ggctatgata tttccgaacc tgcttctaac gcaaaggaag ctgttcaatg
     1441 gacgtatttc ggataccttg cagcaatcaa gacacagaac ggtgctgcta tgagtatcgg
     1501 acgtatcgct actttccttg acatttatat caaacgtgat atggataagg gaatccttac
     1561 agagaaagaa gcgcaggaat taatcgacca tatgactatg aagttccgta tggttaagtt
     1621 cggacgtatc ccagcttaca accagctctt ctccggtgac cctgtatggg caacacttga
     1681 agttggcgga cttggacagg acggtcgttc catggttaca aaaactgact accgtttcct
     1741 ccacacatta gaaaatatgg gaccatcccc agagccaaac ctcacagtac tttactctga
     1801 gcgtcttcca aaagcattta gagattacgc agctcatatt tctattacaa caagttcaat
     1861 ccagtatgaa aatgatgatg taatgcgtcc agtatggggc gatgattact ccatctgctg
     1921 ctgtgtttct gctacacaga ctggtaagga aatgcagttc ttcggtgctc gcgctaacct
     1981 tgcaaaatgt ctcctttacg ctatcaatgg tggtgtggat gaaaagaccg gacagcaggt
     2041 tggaccggaa tataaaccga ttacttccga atatcttgac tatgatgaag taatgcacaa
     2101 atatgatatc atgatggact ggttagcagg tttatatgta aatacattaa atctcatcca
     2161 gtacatgcat gacaaatatt actatgaata tgctttgatg gccttaatcg atacaaatgt
     2221 acgtcgtaca ttcgctacag gtatcgcagg attctcacac gtagtagact ccttaagtgc
     2281 tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngttaa
     2341 gacagttcgt aacgaagaag gactcgttgt agattatgaa acaactggcg acttccctaa
     2401 atacggaaat gatgacgacc gcgccgatga tatcgctgta tggttacttc agacattcat
     2461 gaagaaactg gagaagttcc atacttatcg tgattctgaa cctacaactt ctatccttac
     2521 tatcacatct aacgtagtat atggtaaggc aacaggttcc cttccagatg gaagaaaagc
     2581 cggtgaacca ctttccccag gagcaaaccc aagttatggt gcagagcaga acggtctctt
     2641 agcttcctta aactctgtag ctaaacttcc ttatgaatgg gcattagatg gtatttccaa
     2701 tacgcagaca atcaaccctg atgcactcgg tcattccgaa gaagaacgta ttggtaactt
     2761 agtaaatgtt atggacggct acttcgcaca gggtgctcac cacctaaacg taaatgtatt
     2821 tggtgttgag aaactgattg atgctatgga acatccggaa aaagaagaat acgcaaactt
     2881 tacgatccgt gtttcaggtt atgcagttaa attcatctct ctgacaaaag aacagcagtt
     2941 agatgtaatt gccagaacat gccatgacag aatgtaataa aaatgcgatg gctcttgttc
     3001 attctacgga gagttttggt tcggtggatg ggccgggagt tcgttttatt gtttttttac
     3061 agggttgtcc tttgcgatgt cagttctgtc acaatccaga tacatggaag atgacagagg
     3121 aaaatggtgc ggtgtggaga agtgcggatg agctgttaaa tcaggcactc cgttatcgta
     3181 cttactggaa aaatagcggc ggtattaccg taagtggtgg tgagcctctt ttacagattg
     3241 attttatgct agagtttttt aaaaaggcaa aagaaaaagg aatccacact gttattgata
     3301 ctgctggagg accttttaca agagaggaac ctttcttttc taaatttcag gaattgatga
     3361 aatatacaga cctgttatta gtggatatca aacacattga taacaaatac cacaaagttt
     3421 taactggtcg tagcaatgag aatattcttg atatgattcg ctatctttcc gatatcaaaa
     3481 aaccgatttg gattcgccat gtccttgtcc cggaacgaag cgataaagat gaatatttga
     3541 ctagattagc tgatttcatt cactctcttg ataatgtgga aaaggtggaa atcctgcctt
     3601 atcatacgat gggtatttat aaatggaaag aacttggatt agaatatcct ttagaaggaa
     3661 ttgaccctcc aacgaaagaa cgagtggaga acgccaagaa aattcttgct atttgatttg
     3721 ccgacaaaca gaatcctaaa accccccata tttgtgactt tgaagataga ttgcgttgaa
     3781 tacttattca tcttcaatct atccttgaag tcacaatatg gggtgctctt tagatataaa
     3841 cttcagttaa aattcgctca aattaaattc t
//
