LOCUS       c00043_gnlPro..         7705 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MKNAAJBL_52.
ACCESSION   c00043_gnlPro..
VERSION     c00043_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 20:47:56
            Original ID  :: gnl|Prokka|MKNAAJBL_52
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7705
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..144)
                     /locus_tag="ctg43_1"
                     /transl_table=11
                     /translation="MLFNYTTHFLVLYYFSSSSPPKVRGVREELKLFLFYFFISTFYIS
                     LKK"
     protocluster    1..7705
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[4473:7444]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      4474..7444
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7705
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7705
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             161..334
                     /locus_tag="ctg43_2"
                     /transl_table=11
                     /translation="MKREIIAYKGYFKEFFENLDAGTQDKILYVLKTEITPKKEIDKAK
                     ILRKEYYGSKNK"
     CDS             315..620
                     /locus_tag="ctg43_3"
                     /transl_table=11
                     /translation="MEAKTNEEFFNVSALIDERFGKEGTASRAEAEEKAYAFYTGQIIE
                     DARKKAKITQAELARRIGSDRSYISRVESGQTEPKVSTFYRIMNALGCKIEFSMIL"
     CDS             complement(690..2804)
                     /locus_tag="ctg43_4"
                     /transl_table=11
                     /translation="MKHFTIFQGFYYAIAEMTEEEIVSTIGSFTYREKVEEIRRIFAEQ
                     GEKAANEKKKELPAIAFSASYRGRRTKVNLVKYLGHIVIDIDHLSKEELARILPIIKRC
                     DYTRIAFISPKGMGVKIIVRACHPDETLPETLQEIEDFHHAAYTRLVSFYTELCQIEID
                     TSGQDVARTCLFSYDPEIYFNPNADAFLVDQPQASYKVSNRKNLSGSKQQTPPDGPPTN
                     EDTALNAHSANASLVLTLTYYHNKSEKYIAGNRNNYLHHLSCTFNRYGIPQEEASAFIK
                     SQFTDFPADETASLINSAYAHTDEFNTCKLNGTQKRILRIEQYISEHYETRYNEVLHIM
                     EYRRRHPDTEKPEPFRILDEMMENSIWIEINELGYPCTVKTIENLIYSDFSQSYHPIRE
                     YFELLPEWDGTDYIRILADSVQTSHQEFWAECLERYLVAMCAAATQENIVNHTVLLLCS
                     EIQNIGKTTFINNLLPPELRAYLSTGLINSNNKDDLAKITQAMLINLDEFEGMSGRELN
                     AFKDLVTRKVISFRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHPYSIQF
                     ITINYAQLYAQIKYLLNKPGYQYWFTQADNERLEENNEAFIFHSPEEEMVLTHIRKPER
                     FEKVYYLTVSEIAELIRERTGYQYSIGSKIQLGKVMTKHHFESKKGNNGRRYAVFIIDI
                     EQVKSNRLYE"
     CDS             complement(2891..3121)
                     /locus_tag="ctg43_5"
                     /transl_table=11
                     /translation="MNYKEDSFLIRTYTKAELAHLYNPHVCLKVALQILRRWIIYNLPL
                     LHELEQEGYRARNRLLSPKQVATIIHFLGEP"
     CDS             3315..3818
                     /locus_tag="ctg43_6"
                     /transl_table=11
                     /translation="MAMTVSYSVVPRKNPAKKSEPAKYYAQAQASGELDFEELCEAITS
                     RSTCTETDVRAAISGILYEVKRALKAGRIARLGDLGSLQIGLNSEGAASVKEFSGSMIK
                     GAHLIFRPGKTLAELMKILSYQQVQTRAVAQAGAGDGGGEDPDKNPDSGGDGSGDEEAP
                     DPTV"
     CDS             3878..4045
                     /locus_tag="ctg43_7"
                     /transl_table=11
                     /translation="MKEIVNRILDVIMYLIPFFGKRKRDRVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             4032..4481
                     /locus_tag="ctg43_8"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDEDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(4474..5202)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.5;
                     E-value: 1.4e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg43_9"
                     /sec_met_domain="Radical_SAM (E-value: 1.8e-29, bitscore:
                     92.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(5216..7444)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg43_10"
                     /sec_met_domain="Gly_radical (E-value: 6.2e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1173.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERENNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     KFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLN
                     RDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 tttttttaga gaaatataga aagtagaaat aaagaaataa aatagaaaaa gtttcaattc
       61 ctctctcact cctctcactt ttgggggtga agatgatgaa aaataatata aaacgaggaa
      121 atgtgttgta tagttgaaca acatttctta tatttgcgtt atgaaaagag aaataatagc
      181 atataaagga tacttcaaag aattctttga aaatttagat gctggcaccc aagataagat
      241 attgtatgtg ctgaaaaccg aaataacacc aaagaaagaa attgataaag caaagatatt
      301 aagaaaggag tattatggaa gcaaaaacaa atgaggaatt ctttaatgtc agtgcattaa
      361 tagatgagcg ttttggtaag gaaggaacag cttctcgtgc agaggcagaa gaaaaggcct
      421 acgcctttta tacaggacag attatcgagg acgcaagaaa aaaggctaaa atcactcagg
      481 ctgaattagc ccggcgtatc ggttctgacc gttcttatat ttcaagagta gaaagtggtc
      541 agacagagcc taaagtatct actttctatc gtataatgaa tgcgttaggc tgtaaaatcg
      601 aattttcaat gattttgtaa aaacaataaa tgcagagtat ctgtcaagat gactctgcat
      661 tttgctatat tcacaaggct ttcccgcttc tattcataca gcctgtttga ttttacctgc
      721 tcgatatcta taataaatac ggcatatcgg cgcccgttat ttcctttttt agattcaaaa
      781 tggtgcttag tcatcacctt ccccaactgt atttttgatc cgatggaata ctgataaccg
      841 gtacgctcac gtattagttc cgcaatctca ctgacagtca gataataaac cttctcgaaa
      901 cgttctggtt tgcggatatg cgtaagaacc atttcttctt ccggtgaatg aaagatgaaa
      961 gcctcgttat tctcttcaag ccgttcattg tccgcttgcg tgaaccagta ttgatatccc
     1021 ggcttattca gcagatattt gatctgagca tagagctgcg cataattgat ggtaataaat
     1081 tggatggaat aaggatgaaa acaaaggaaa cgccggttgc ctgtcgtgtc gtgaagcact
     1141 tcctgataat tgcaggttcc tgcaaaagag gccgtatgag ggaagttttg cgaacggcgt
     1201 gcgtaaggca gacggaaact gatcactttg cgcgttacca aatctttgaa tgcattcagt
     1261 tcccggccgc tcatcccttc aaactcgtcg agattgatca gcatagcctg cgttattttg
     1321 gctaaatcat ctttattgtt cgaattgatt agtccggtag agagataagc acgcagttcg
     1381 ggaggcagga gattattgat aaaggtagtc tttcctatgt tctgtatctc gctgcatagc
     1441 agaagtaccg tgtgattgac aatattctcc tgagtagccg ctgcgcacat cgccaccaga
     1501 taacgttcca ggcattccgc ccaaaactcc tgatgactcg tctggacact atccgccagg
     1561 atacggatat agtcggttcc gtcccattcg ggcagtaatt caaaatactc gcgaatggga
     1621 tgatacgact ggctgaaatc ggagtaaatc agattttcga ttgttttgac agtgcaggga
     1681 tatcccagct cgttgatttc tatccagata ctgttttcca tcatctcatc caagatgcgg
     1741 aaaggctccg gtttctcggt gtcgggatgc cttctgcgat attccatgat atgcagcact
     1801 tcgttgtagc gtgtttcgta gtgctcgctg atatattgct ctatccgtaa tattcgtttc
     1861 tgcgtgccat tcagtttaca ggtattaaat tcgtcggtat gagcgtaggc gctattgatc
     1921 aaagaggctg tctcgtccgc cggaaagtct gtaaactggc ttttgataaa ggcggatgct
     1981 tcctcctgcg gaatgccgta gcgattgaag gtacatgaga gatggtgcag ataattgttc
     2041 cggttgcccg ctatgtattt ttcggacttg ttgtgatagt aggttaaagt cagcaccaga
     2101 gaagcgttag cggaatgggc gttgagcgct gtgtcttcat tcgtcggggg gccgtccgga
     2161 ggagtctgtt gtttactgcc ggagagattt ttacgatttg aaaccttgta ggatgcctga
     2221 ggttgatcta caagaaaagc gtcagcgttc ggattgaaat aaatctccgg atcgtaagag
     2281 aacagacaag tgcgtgccac atcctgaccg gaggtgtcga tttctatctg acagagttcg
     2341 gtgtaaaaag aaacgagtct ggtgtaagct gcatgatgga agtcttctat ttcctgaagg
     2401 gtttcgggca atgtctcatc cggatggcat gcgcgtacta tgattttcac gcccatccct
     2461 ttggggctga taaaggcgat gcgggtgtaa tcgcatctct tgatgatagg gaggatgcgt
     2521 gccaattctt ctttgctgag gtggtcgatg tctatcacga tgtgtcccag atatttgacg
     2581 aggttcactt tggtacgcct tcctcggtag ctggcggaga aggcgattgc cggcaattct
     2641 tttttcttct cattggcagc cttttctcct tgctctgcaa agatgcggcg tatttcttcg
     2701 actttttccc ggtaggtaaa actgccgata gtggacacta tttcttcttc ggtcatctct
     2761 gctattgcgt agtagaaacc ttgaaatatg gtgaaatgtt tcatttttat tttgattatt
     2821 gtttgtcgat gcaaaggaac ttctttgcgg aaatgtgtaa aggaactttg tataaaatgg
     2881 caataagtta ctacggttcg cccaaaaagt gtatgatagt tgccacttgt ttcggcgaaa
     2941 ggagacggtt gcgtgcgcgg tagccttcct gttcgagttc gtgcaggaga gggaggttgt
     3001 aaataatcca acgacgaagt atttgtaaag caactttgag gcagacatgg ggattgtaaa
     3061 ggtgagccag ttccgctttg gtataggtgc ggataaggaa agaatcttct ttgtaattca
     3121 tgatgttttc ttcttgtttt atgctataaa gttacataaa attccttgtt attcaaaggt
     3181 ttgacttcgt ttttattcgt tgggaatcaa gaaacttcgt tggggaatat ggcttgtccg
     3241 gctatcctta tctttgtgct gttaacaaga aggggatgca tcctatgtat tataaacttt
     3301 aaaattattt tattatggca atgacagtat cttattcagt ggtgccgcgc aagaatcctg
     3361 caaaaaagag cgaaccggca aagtattatg cacaggcgca ggcgtcgggt gagctggact
     3421 ttgaagagtt gtgcgaagcg atcaccagtc gttctacttg tacggaaacg gatgtacgtg
     3481 cggctatttc gggtattctt tacgaagtga aacgtgcgtt gaaggcagga agaattgcga
     3541 gactgggcga tttgggcagt ttgcagatag gactgaacag tgaaggtgcg gcatcggtca
     3601 aggaattttc cggttcaatg attaaggggg cgcatcttat tttccgcccc ggtaagacgc
     3661 tggcggagtt gatgaagatt ctgagttatc agcaagtgca gactcgtgcg gtggcgcagg
     3721 cgggtgcagg cgatggcggg ggagaagacc ctgacaagaa tccggattcc ggtggtgatg
     3781 gctccggtga tgaagaggct cccgacccta cggtataatt ggcagacggg tcaaggtaat
     3841 aacgaattag aataacttcc attaatattt tgataagatg aaagaaatag taaaccggat
     3901 tttagatgtg atcatgtatc tgattccttt cttcggaaag agaaagcggg acagggtggt
     3961 gagagaagtg cgctatcata ctacctgtaa ggaggtgtgc aaagtgaaaa cgacggaaag
     4021 ggaaaaggaa catgagaaag attagtctga ttgtgatcca ttgttccgcc acccgtgtcg
     4081 attgcgactt tacggcaaag gatgtggata cggctcaccg ttatcgtggc ttttcgtgct
     4141 gggggtatca ttattatata cgcaagtcgg gcgagattga gccgatgcgg gatgaagata
     4201 cggtcggagc tcatgcacgt ggctataatg cgatcagctt aggtgtatgt tatgagggcg
     4261 gtctggatga gaatggaaaa gctgcggata cgcgtacacc ccgtcagaag gaggcgctac
     4321 atcgcttggt acacgaattg ttgcagcgtt atccggaagc taaagtagta gggcatcggg
     4381 atttaagtcc cgacaccaac tataatggga tcgtagatcc ttgggaacgg attaaagaat
     4441 gcccttgctt tgaggtcatt ggagaattct ccattaatta actaccaccg tagtgaaata
     4501 ctctttgaat acctccgcag ctttttcaag ctgttcgggg gtattttctt ttaccccttt
     4561 catcttgtat tcctgtccca tcgcttcgta cttatgtacg cccagtgtat ggtaaggtag
     4621 tatttccacc cgttggatca tcttgtattt tccgagtgct tctcccagtg cgcgtatgtc
     4681 ttcttcaaaa tcgctgtatc cgggcactag cacgtagcgt agccaaaaag gtttctcctg
     4741 ttcttccagc caggcggccg tgcggatggt ttgctcgttg cttcttccgg tgagcgtctg
     4801 atggcggttg gggttgaatt cctttatatc caataacaca agatcggtca gtctgaataa
     4861 ttcttctacg tcttcgttcc aaagtcctcc gttgctgtcc agacagacat ggatgccttt
     4921 ttctttcagt tcgcggacca gcgggacgag tgcttttgcc tgaaacgtcg gttcgcctcc
     4981 ggagaaagtg attcctcctc gtttcccgaa gaaggggcgc tggctcattg ccatgcggac
     5041 gatttcttcc ggtggggtag gcgtgcctcc ctttcctgct atcgtatccg gattggcgca
     5101 atagaggcag cggaagttgc atccttgaag aaaaacgacg agccgtaagc ccggcccgtc
     5161 gaatgttccc atactttcgt atgaatgtac gtttatcatc atatagagta atagattaca
     5221 tacgttcgtg gaagctacgg ctgataactt ccagttggtg ttcgcggctc agcttcacga
     5281 agtttacggc atagccggaa acgcggatag tcagctgcgg atagttttcc ggatgctcca
     5341 tggcatcgta cagcatatca cggttcagta cgtttacgtt caggtggtgg gcacctttgg
     5401 tgaagtaacc gtccatcatc gttacgaggt tctcgatacg gtcttcgtcc gtagcaccca
     5461 gtgatttcgg aacgatagag aaggtgttgc tgataccgtc ctgtgagtca cggtaacgaa
     5521 gtttcgctac agagctcaga gaagcgatcg caccattctt gtcgcgtccg tgcatcgggt
     5581 tggcacccgg agcgaaggca acacctttgg cacgtccgtc gggagtagca ccggtctttt
     5641 tgccgtacat cacattggaa gtgatagtca gcaaggagag ggtaggacgg gcattcttgt
     5701 aaacaggcaa tttcttcaat tcttcgctga agaagtatac caggtctacg ccaaggtggt
     5761 ctaccttgtc gttgtcgtta ccgaagcaag ggaattctcc ttcgatgtcg aagccttctg
     5821 tcagaccgat atcattgcgg cgggcagtta ctttcgcata tttgatggca gacagtgaat
     5881 cgagggcgat ggaaagtccg gctacaccgt aagcaaggtt tatgcgcggg ttcgtatcta
     5941 caagagccat ctgggccttc tcgtagtaat acttgtcgtg catatagtgg atgatgttca
     6001 tcgcttcgtt atatacgcgg gcgatttcgg tcagcacttt tttgtagttg tccattactt
     6061 cttcgaactt cagcgtgtcg ctggtcagca cggggatgtt cttcaccatg actgtaccgg
     6121 tattctcgca acgtccgccg ttgatggcaa gcaacagggc tttggccagg ttgcaacggg
     6181 cgccgaagaa ctgaatctgc ttacctattt cctggtatga tacgcagcag gcgattccgt
     6241 agtcgtcgga ctgacgtact tcgcgcatca ggtcgtcatt ttcgtactgg atagaagaag
     6301 tgtcgataga aacctttgca cagaattctt tgaatccttc cggaagttcc gggctccaga
     6361 gtacggtcag gttcggttcc ggtgaagggc cgaggttgta caatgtctgc aggaaacgga
     6421 aggaggtctt tgtcactttg gtacgtccgt cgttgagacg tccgcccaga gattcggtta
     6481 cccaggtcgg gtcgcctgca aagatgtcgt tgtatgattg catacgcagg tggcggacca
     6541 tacgcagttt gatgacaaac tggtcgatca gctcttgcgc gaatgattcg gtgatggttc
     6601 ctttgctcag ttcatattcc agatagatat cgaggaaaga agaaacgtta cccagtgaca
     6661 tagcggcacc gtcttgttct ttgacggcgg caaggtaagc catatatacc cactgtacgg
     6721 cttcttgtgc cgtgtaagca ggacggctca ggtcgagacc gtagtattcg cccatcactt
     6781 tcatgtcttt cagtgccttg atctgttctg ccacttcttc gcgcaggcgg atgcgggcct
     6841 ctgtcatcgg accggtgagg ttgtgcaaat cttccttctt tgcttcgatc agacggtcga
     6901 tgccgtaaag agccatacgg cggtagtcac cgatgatgcg tccgcgtgca taattgtcgg
     6961 gaagtccggt aaggaatccc agcgaacgga aggaacggat ttcttccgta tatacgtcga
     7021 atactccgtc gttgtgcgtc ttgcggtagt gagtgaaaat atcttttacg cggtcgtcca
     7081 cttccacgcc gttttcgtga caggctttgc tgactacgtt gatacctccg aaaggcttga
     7141 tggcacgttt cagaagttcg tctgtctgca ggccgacgat cagttcgttt tccttgtcga
     7201 tatatccggc cttgtgggaa gtgatggttg atacggtaac attatctaat gagcggacac
     7261 cgttgttttc tctttcttct gccaacgctt cgaggcagcg gttccatacg gctttggtac
     7321 gttctgtagg tccttcgagg aatgaagcat ctccgtaata cggagtgatg ttatgactta
     7381 cgaaatctct gacattgatt tcgctgctcc aaagaccgtc tttaaagatc ttgtttaatt
     7441 ccataaaatg ataaaattag aggttgtagt tatcctattc tttagagcgc acaaagttac
     7501 aatcattttt catataatca gcataaatat gctctatttt atgcttttca acataaaata
     7561 cctattaatt gctatcctta ggaaaagatt agattaaatt tttagtgaaa actcttgcaa
     7621 gtttaaaata tcttcgtacc tttgcaccgc ttttaacgaa aagcacttct gataaggaag
     7681 ttttggagag gtggcagagt ggtcg
//
