GCA_000569035.1 - 2 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaMDBLGAPE_1 (original name was: gnl|Prokka|MDBLGAPE_1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	hglE-KS	225,124	267,652
Region 2	lassopeptide,lanthipeptide-class-iii	1,819,503	1,850,777

gnlProkkaMDBLGAPE_1 - Region 1 - hglE-KS

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Location: 225,124 - 267,652 nt. (total: 42,529 nt) Show pHMM detection rules used

hglE-KS: (hglE or hglD)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 225123 - 267652:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_182

PKS_AT (38..333): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 53.5
Methoxymalonyl-CoA: 42.7
Methylmalonyl-CoA: 21.4
inactive: 13.9
2-Methylbutyryl-CoA: 13.9
Propionyl-CoA: 11.0
Isobutyryl-CoA: 10.1
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

gnlProkkaMDBLGAPE_1 - Region 2 - lanthipeptide-class-iii,lassopeptide

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Location: 1,819,503 - 1,850,777 nt. (total: 31,275 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)
lanthipeptide-class-iii: (micKC and not PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Lanthipeptides

Lasso peptides

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Lasso peptide predictions

Lassopeptide(s) for ctg1_1546

ctg1_1546

Leader:

MKQYIAPTVETIASFREATN

Full core:

GLWFGKYVDIGGAKAPFPWGSN

Putative macrolactam:

GLWFGKYVD

MIBiG 3.1 matches for ctg1_1546

11.8% BGC0000535 (ADJ56352.1): Lanthipeptide: nisin A

GAKAPFPWGSN

G K G N

GCKTGALMGCN

antiSMASH-DB 4.0 matches for ctg1_1546

100.0% NZ_CP006714.1: Lassopeptides: B2258_RS10665_lassopeptide

GLWFGKYVDIGGAKAPFPWGSN

86.4% NZ_CP062951.1and 1 other:

NZ_CP071248.1 Lassopeptides: BLI009_RS05010_lassopeptide

Lassopeptides: BL1347_RS03935_lassopeptide

GLWFGKYVDIGGAKAPFPWGSN

G+WFG YVD+GGAKAPFPWGSN

GVWFGNYVDVGGAKAPFPWGSN

50.0% NZ_KI912489.1: Lassopeptides: allorf_513617_513742_lassopeptide

GLWFGKYVDIGGAKAPF

GLW+GK+ DI GAK F

GLWYGKFRDIFGAKTYF

with 13 more sequence matches

47.8% NZ_NCVL01000001.1and 1 other:

NZ_VMMF01000036.1 Lassopeptides: HT717_RS09510_lassopeptide

Lassopeptides: B7694_RS10650_lassopeptide

GLWFGKYVDIGGAKAPFP

GLWFGK+ DI G +A F

GLWFGKFTDIFGGRAVFQ

40.9% NZ_KI912489.1: Lassopeptides: allorf_513868_514041_lassopeptide

GLWFGKYVDIGGAKA

G+WFGK+ DI G +A

GVWFGKWTDIFGGRA

39.1% NC_012468.1and 9 others:

NZ_CGXY01000023.1 Lassopeptides: AQD87_RS12295_lassopeptideNZ_CLXO01000005.1 Lassopeptides: AFA99_RS11895_lassopeptideNZ_CMLU01000007.1 Lassopeptides: AEP96_RS12330_lassopeptideNZ_CP014326.1 Lassopeptides: AXK38_RS11095_lassopeptideNZ_CRCU01000004.1 Lassopeptides: AYW54_RS12245_lassopeptideNZ_FDQP01000002.1 Lassopeptides: A1S42_RS12075_lassopeptideNZ_FHTX02000015.1 Lassopeptides: A0693_RS10110_lassopeptideNZ_FZBS01000003.1 Lassopeptides: CFY35_RS11490_lassopeptideNZ_RXXR01000008.1 Lassopeptides: EKA98_RS05595_lassopeptide

Lassopeptides: SP70585_RS13230_lassopeptide

GLWFGKYVDIGGAKA

GLW GK+ DI G +A

GLWTGKFRDIFGGRA

39.1% NC_021023.1and 26 others:

NZ_BJED01000004.1 Lassopeptides: FFR60_RS05785_lassopeptideNZ_CABGPX010000005.1 Lassopeptides: FN189_RS04890_lassopeptideNZ_CABGTR010000001.1 Lassopeptides: FN307_RS01775_lassopeptideNZ_CABGXT010000046.1 Lassopeptides: FN373_RS15575_lassopeptideNZ_CP045045.1 Lassopeptides: F9293_RS05110_lassopeptideNZ_CP046111.1 Lassopeptides: GKQ56_RS05625_lassopeptideNZ_CP063980.1 Lassopeptides: ISN59_RS06410_lassopeptideNZ_CP097069.1 Lassopeptides: M2912_RS09265_lassopeptideNZ_CP110068.1 Lassopeptides: OLM02_RS09865_lassopeptideNZ_CP116962.1 Lassopeptides: PO880_RS04740_lassopeptideNZ_GG670372.1 Lassopeptides: EFDG_RS21040_lassopeptideNZ_GL457197.1 Lassopeptides: HMPREF9517_RS19205_lassopeptideNZ_JADOEP010000002.1 Lassopeptides: IW492_RS01515_lassopeptideNZ_JADPEP010000046.1 Lassopeptides: I4U96_RS13395_lassopeptideNZ_JTKS01000001.1 Lassopeptides: QI17_RS14045_lassopeptideNZ_JTKX01000023.1 Lassopeptides: QR19_RS15990_lassopeptideNZ_KB932331.1 Lassopeptides: Q9A_RS18260_lassopeptideNZ_KB944632.1 Lassopeptides: WMI_RS18340_lassopeptideNZ_KB944658.1 Lassopeptides: WMM_RS18400_lassopeptideNZ_KB944694.1 Lassopeptides: WO3_RS18855_lassopeptideNZ_KB944828.1 Lassopeptides: WOM_RS18345_lassopeptideNZ_KE351156.1 Lassopeptides: D924_RS19170_lassopeptideNZ_KE352620.1 Lassopeptides: D350_RS18085_lassopeptideNZ_NAQY01000001.1 Lassopeptides: B6A11_RS15210_lassopeptideNZ_SCHR01000003.1 Lassopeptides: EQ847_RS03410_lassopeptideNZ_SMGV01000009.1 Lassopeptides: EYY48_RS11015_lassopeptide

Lassopeptides: ENT_RS07390_lassopeptide

GLWFGKYVDIGGAKAPFP

GLW GK+ D+ G +A F

GLWTGKFRDVFGGRALFQ

39.1% NZ_AULE01000001.1: Lassopeptides: H509_RS27430_lassopeptide

GLWFGKYVDIGGAKA

GLW GKY D+ G +A

GLWTGKYTDVFGGRA

39.1% NZ_KI912489.1: Lassopeptides: allorf_514674_514802_lassopeptide

GLWFGKYVDIGGAKA

GLW GK+ DI G +A

GLWTGKWTDIFGGRA

36.4% NZ_CP053549.1and 3 others:

NZ_JADKSM010000003.1 Lassopeptides: ITJ61_RS18070_lassopeptideNZ_PSXX01000017.1 Lassopeptides: C5E05_RS18625_lassopeptideNZ_PSXZ01000003.1 Lassopeptides: C5E07_RS02640_lassopeptide

Lassopeptides: HMI60_RS00570_lassopeptide

GLWFGKYVDIGGAKA

G+WFG++ DI G +A

GVWFGRWRDIFGGRA

36.4% NZ_CP106992.1: Lassopeptides: NDS46_RS23370_lassopeptide

GLWFGKYVDIGGAKA

GLW GK+ D+ G +A

GLWTGKFKDLFGGRA

36.4% NZ_JAMDLY010000010.1and 4 others:

NZ_JAPDMW010000034.1 Lassopeptides: OF778_RS29475_lassopeptideNZ_JMLT01000016.1 Lassopeptides: BP95_RS33025_lassopeptideNZ_LS992241.1 Lassopeptides: PBLR_RS07315_lassopeptideNZ_SADY01000011.1 Lassopeptides: C7Y44_RS26425_lassopeptide

Lassopeptides: M5X04_RS09885_lassopeptide

GLWFGKYVDIGGAKA

G+WFG+Y D+ G +A

GIWFGRYRDVLGGRA

36.4% NZ_NGKY01000001.1: Lassopeptides: A5819_RS19010_lassopeptide

GLWFGKYVDIGGAKA

G+WFG++ D+ G KA

GVWFGRWKDVFGGKA

34.8% NZ_FILX01000006.1and 1 other:

NZ_JAGFVF010000023.1 Lassopeptides: allorf_10827_10955_lassopeptide

Lassopeptides: allorf_42148_42276_lassopeptide

GLWFGKYVDIGGAKA

GLW GK+ D+ G +A

GLWTGKFRDVFGGRA

31.8% NZ_CP009452.1and 1 other:

NZ_NISJ01000009.1 Lassopeptides: CDQ91_RS21055_lassopeptide

Lassopeptides: LH20_RS23250_lassopeptide

FGKYVDIGGAKAP

FG V+IGG + P

FGPPVEIGGHELP

27.3% NZ_LMUL01000017.1: Lassopeptides: allorf_410478_410597_lassopeptide

GKYVDIGGAKAPFPWG

G +V+ K P P G

GDFVETALTKQPIPMG

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Lasso peptides

Core peptide predictions

No core peptides found.

Core peptide predictions

ctg1_1546 - Class II: Cleavage pHMM score: -8.10; RODEO score: 18; Monoisotopic mass: 2348.2 Da; Molecular weight: 2349.6 Da; Number of bridges: 0