LOCUS       c00656_gnlPro..         4231 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GKGENLLJ_1454.
ACCESSION   c00656_gnlPro..
VERSION     c00656_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-03 17:15:51
            Original ID  :: gnl|Prokka|GKGENLLJ_1454
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4231
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4231
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1256:4229]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1257..4229
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4231
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4231
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             61..564
                     /locus_tag="ctg656_1"
                     /transl_table=11
                     /translation="MAMTVTYSVVPRKNPAKKDESAKYYAQAQASGELDFEELCEGITS
                     RSTCTETDVRAAISGILYEAKRALKAGRIVRLGDLGSLQIGLNSEGAVSVKEFSSSLIT
                     AAHIIFRPGKTLADITKILSYQQVTTRAVAQTGGSGNEGEDDDKGSGGGSDGGGSGEAP
                     DPAA"
     CDS             585..752
                     /locus_tag="ctg656_2"
                     /transl_table=11
                     /translation="MKEIVTKILDVIMFLVPFFGKRKRNRIVREVRFNATHKEVCNVKT
                     TEREKDDEKD"
     CDS             739..1185
                     /locus_tag="ctg656_3"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRADRDFTAKDVDTAHRFRGFSCWGYHYYIRK
                     SGQIEPMRDEDTVGAHARGFNAISLGVCYEGGLDENGKAADTRTSRQKEAMHRLVSELL
                     QRYPDAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIEEFES"
     CDS             complement(1257..1985)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 73.2;
                     E-value: 8.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg656_4"
                     /sec_met_domain="Radical_SAM (E-value: 2.7e-30, bitscore:
                     94.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLMRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRRLGEALGKYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(2001..4229)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg656_5"
                     /sec_met_domain="Gly_radical (E-value: 2.1e-47, bitscore:
                     148.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1171.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSTEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERANNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEVKKEDLRNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYMEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     NFEEVMSNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIQGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATEEDRVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLYDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 tatgtcgtta acaaataagg aacgcgttcg aaatgtgtga aatcttaaaa ttattttatt
       61 atggcaatga cagtaactta ttcagtagtg ccgcgcaaga atcctgccaa aaaggatgag
      121 tcggcaaagt attacgcgca ggcacaggct tcgggagagt tggattttga agagttgtgc
      181 gagggaatca cgagtcggtc tacctgtacg gagacagatg tgcgtgccgc catttcgggt
      241 attctttatg aggcgaagcg tgcgttgaaa gcgggaagaa tagtccggtt gggtgatttg
      301 ggcagtttgc agatcgggtt gaacagtgaa ggagctgtgt cggtcaagga gttttcaagt
      361 tcgctgatta cggctgccca tattattttc cgtcccggaa agacgttggc ggacatcacg
      421 aagattctta gctaccagca ggtaacgacg cgtgcggtgg cccaaacggg cggtagcggc
      481 aatgaaggag aagacgatga caaaggttcc ggtggcggtt cagacggtgg tggaagcgga
      541 gaagctccgg atccggcagc atgataactt taatattaga tcaaatgaaa gaaattgtaa
      601 ctaagatttt ggatgtaatc atgttccttg tgcctttctt cggaaaaaga aagcggaaca
      661 gaattgtgag agaggtgcgc ttcaatgcta cccataagga agtgtgtaat gtgaaaacaa
      721 cggaaaggga aaaggacgat gagaaagatt agtctgattg tgattcactg ctctgctacc
      781 cgtgctgatc gcgacttcac ggcgaaggat gtggacactg cccatcgctt tcggggcttt
      841 tcttgttggg ggtatcatta ttatatccgc aagtcgggac agatagaacc gatgagggat
      901 gaagatacgg tgggtgctca tgcacgcggt tttaatgcga ttagtttagg ggtgtgctat
      961 gaaggcgggc tggatgagaa cggaaaggca gcagatacgc gtacttcccg ccagaaagag
     1021 gcgatgcatc gtctggtaag cgagttgttg cagcgttatc cggatgcaaa ggtggtcggt
     1081 caccgggact tgagccctga caccaattat aatggaattg tagacccctg ggaacggatc
     1141 aaagagtgtc cctgttttga ggtgattgaa gaatttgaga gttgagaatg gagagttgag
     1201 agttggctgc gcactgttat actgcatagt taactctcaa ttctcaactt cttcaactaa
     1261 ttgaccacca cagtagtgaa atattcttta aatacctccg cggctttttc aagttgttcg
     1321 ggggtatttt cttttacgtc tttcagcttg tattcctgct ccatcgcttc atacttatgt
     1381 acgcctaatg tatgataagg caggatttcc actcgttgca ccatcttata ttttcccaga
     1441 gcctctccta aacggcggat gtcttcctcg aaatcgctgt atccgggcac taatacataa
     1501 cgcagccaga aaggtttctc gttttcttcc agccatgctg ccgtgcggat ggtttgctcg
     1561 ttacttcttc cggtcagtgc ctgatggcgt gccggattga actctttaat atccagcaat
     1621 acaagatccg tcagtttgaa aagttcttct acttcttcgt tccagatacc gccgttgctg
     1681 tctatgcata catgtattcc cttttctttc agttcacgca ttaaagggac tagtgctttg
     1741 gcctgaaacg taggttctcc tccggagaaa gtgactcctc cgcgtttccc gaaaaaagga
     1801 cgttggctca tagccatgcg gacgatttct tccggtgggg taggtgtgcc tccttttccg
     1861 gctatcgtgt cggggttggc acaatacagg cagcggaaat tgcatccttg aagaaaaacg
     1921 acgagccgta agcccggccc gtcgaatgtt cccatacttt cgtatgaatg tacgtttatc
     1981 gtcatattga gtagatctga ttacatacgt tcgtggaagc tacggctgat aacttccaat
     2041 tgatgttcgc ggctcaactt cacgaagttt acggcataac cggaaacacg gatggtgagt
     2101 tgcggatatt tttccggatg ttccatggca tcgtaaagca tttcacggtt cagtacgttc
     2161 acgttcaggt ggtgagcgcc tttggtgaag taaccatcca tcattgtcac cagattttct
     2221 acgcgatctt cttcagtagc tcccagtgat ttcggaacga tagagaaggt gttgctgata
     2281 ccgtcctgtg agtcgcggta acgtaatttc gctacggaac tcaaagaggc gatggcgccg
     2341 ttcttgtcac gtccgtgcat agggttggct cccggagcaa aagcgacacc tttggcgcgg
     2401 ccgtcgggag tagcacctgt cttcttgcca tacatcacgt tggaagtgat ggtgagcaaa
     2461 gacagagtag gacgggcatt tttgtaaacc ggaagtttct tcaattcttc gctgaagaaa
     2521 tagaccaggt caacacccag gtggtctacc ttgtcgttgt cattaccgaa gcaagggaat
     2581 tcgccttgaa tatcgaaacc ttctgtcaga ccgatgtcgt tgcggcgtgc ggttactttg
     2641 gcatatttga tggccgacag tgaatcgagg gcgatagaaa gtccggctac accgtaagca
     2701 aggttgatgc gcgggttggt atctacaaga gccatttggg ctttttcgta gtaatactta
     2761 tcatgcatat aatggatgat gttcatcgct tcgttgtaaa cacgggcgat ttctgtcaat
     2821 accttcttgt agttgctcat tacttcttcg aagttcagtg tgtcgctggt tagtacgggg
     2881 atgtttttca ccattaccgt gccggtgttt tcgcaacgtc cgccgttgat ggcaagcagc
     2941 agggctttag ccaggttgca acgtgcgccg aagaactgga tttgttttcc gatttcctga
     3001 taagatacgc agcacgcgat tccgtagtcg tcagactggc gtacttcacg catcaggtcg
     3061 tcgttttcat attggatgga agaagtgtcg atagatactt ttgcgcagaa ttctttgaat
     3121 ccttccggaa gttccgggct ccacaataca gtcaggttcg gttccggtga agggccgagg
     3181 ttgtagagtg tttgcaggaa gcggaaagaa gtctttgtca cctttgtgcg tccgtcgttg
     3241 aggcgtccac ccaaggattc ggtcacccat gtcgggtcgc cggcaaagat gtcgttgtaa
     3301 gattgcatac gcaggtggcg caccatacgc agtttgatga caaactggtc gatcagctcc
     3361 tgtgcgaaag attctgtgat agttcctttg ctcaattcat attccatata aatgtcgagg
     3421 aacgaagaga cgttacccag tgacatggca gcaccgtctt gttcttttac ggcagcaagg
     3481 taagccatgt acacccattg tactgcttct tgtgcggtgt aggcaggacg gctcaagtcg
     3541 agtccgtagt attcgcccat taccttcatg tccttcaatg ccttgatttg ctctgccact
     3601 tcttcgcgca ggcggatgcg ggcttcggtc atcggaccgg taaggttacg caaatcttct
     3661 ttttttacct caatcagtcg gtcgatacca tagagagcca tacggcggta gtcgccgatg
     3721 atacgtccgc gggcatagtt gtcaggaagt ccggtgagga atcccagaga acggaacgaa
     3781 cggatttctt cggtatatac gtcaaatact ccgtcattgt gcgtcttgcg gtagtgagtg
     3841 aatatatctt ttacacggtc gtccacttcc acgccgtttt cgtggcaggc tttgctcacc
     3901 acgttgatac ctccgaaagg tttgatggca cgtttcagaa gttcgtctgt ttgcagaccg
     3961 acgatcagtt cgttttcttt gtcgatatat ccggctttat gagaagtgat ggttgagacg
     4021 gtgacattat ccagtgagcg tactccgtta ttggctcttt cttctgccag tgcttcgagg
     4081 cagcggttcc atacagcttt tgtgcgttcg gtaggtcctt cgaggaaaga agcatctcca
     4141 taatacggag tgatgttgtg actaacgaaa tctcttacgt tgatttctgt gctccaaaga
     4201 ccgtctttaa agatcttatt taattccata a
//
