GCA_000598585.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaOEMCJHIA_8 (original name was: gnl|Prokka|OEMCJHIA_8)

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	RRE-containing	157,362	177,715	polysaccharide C	Saccharide	20%

gnlProkkaOEMCJHIA_15 (original name was: gnl|Prokka|OEMCJHIA_15)

Region	Type	From	To	Most similar known cluster		Similarity
Region 15.1	phosphonate	484,045	498,309	polysaccharide B	Saccharide	43%

gnlProkkaOEMCJHIA_16 (original name was: gnl|Prokka|OEMCJHIA_16)

Region	Type	From	To	Most similar known cluster		Similarity
Region 16.1	phosphonate	797	19,891	polysaccharide B	Saccharide	13%

gnlProkkaOEMCJHIA_18 (original name was: gnl|Prokka|OEMCJHIA_18)

Region	Type	From	To	Most similar known cluster		Similarity
Region 18.1	NRPS-like	69,032	111,461

gnlProkkaOEMCJHIA_59 (original name was: gnl|Prokka|OEMCJHIA_59)

Region	Type	From	To	Most similar known cluster		Similarity
Region 50.1	NRPS	48,935	94,107

No secondary metabolite regions were found in these records:: gnlProkkaOEMCJHIA_1 (original name was: gnl|Prokka|OEMCJHIA_1); gnlProkkaOEMCJHIA_2 (original name was: gnl|Prokka|OEMCJHIA_2); gnlProkkaOEMCJHIA_3 (original name was: gnl|Prokka|OEMCJHIA_3); gnlProkkaOEMCJHIA_4 (original name was: gnl|Prokka|OEMCJHIA_4); gnlProkkaOEMCJHIA_5 (original name was: gnl|Prokka|OEMCJHIA_5); gnlProkkaOEMCJHIA_6 (original name was: gnl|Prokka|OEMCJHIA_6); gnlProkkaOEMCJHIA_7 (original name was: gnl|Prokka|OEMCJHIA_7); gnlProkkaOEMCJHIA_9 (original name was: gnl|Prokka|OEMCJHIA_9); gnlProkkaOEMCJHIA_10 (original name was: gnl|Prokka|OEMCJHIA_10); gnlProkkaOEMCJHIA_11 (original name was: gnl|Prokka|OEMCJHIA_11); gnlProkkaOEMCJHIA_12 (original name was: gnl|Prokka|OEMCJHIA_12); gnlProkkaOEMCJHIA_13 (original name was: gnl|Prokka|OEMCJHIA_13); gnlProkkaOEMCJHIA_14 (original name was: gnl|Prokka|OEMCJHIA_14); gnlProkkaOEMCJHIA_17 (original name was: gnl|Prokka|OEMCJHIA_17); gnlProkkaOEMCJHIA_19 (original name was: gnl|Prokka|OEMCJHIA_19); gnlProkkaOEMCJHIA_20 (original name was: gnl|Prokka|OEMCJHIA_20); gnlProkkaOEMCJHIA_21 (original name was: gnl|Prokka|OEMCJHIA_21); gnlProkkaOEMCJHIA_22 (original name was: gnl|Prokka|OEMCJHIA_22); gnlProkkaOEMCJHIA_23 (original name was: gnl|Prokka|OEMCJHIA_23); gnlProkkaOEMCJHIA_24 (original name was: gnl|Prokka|OEMCJHIA_24); gnlProkkaOEMCJHIA_25 (original name was: gnl|Prokka|OEMCJHIA_25); gnlProkkaOEMCJHIA_27 (original name was: gnl|Prokka|OEMCJHIA_27); gnlProkkaOEMCJHIA_28 (original name was: gnl|Prokka|OEMCJHIA_28); gnlProkkaOEMCJHIA_29 (original name was: gnl|Prokka|OEMCJHIA_29); gnlProkkaOEMCJHIA_30 (original name was: gnl|Prokka|OEMCJHIA_30); gnlProkkaOEMCJHIA_31 (original name was: gnl|Prokka|OEMCJHIA_31); gnlProkkaOEMCJHIA_32 (original name was: gnl|Prokka|OEMCJHIA_32); gnlProkkaOEMCJHIA_33 (original name was: gnl|Prokka|OEMCJHIA_33); gnlProkkaOEMCJHIA_34 (original name was: gnl|Prokka|OEMCJHIA_34); gnlProkkaOEMCJHIA_35 (original name was: gnl|Prokka|OEMCJHIA_35); gnlProkkaOEMCJHIA_36 (original name was: gnl|Prokka|OEMCJHIA_36); gnlProkkaOEMCJHIA_39 (original name was: gnl|Prokka|OEMCJHIA_39); gnlProkkaOEMCJHIA_40 (original name was: gnl|Prokka|OEMCJHIA_40); gnlProkkaOEMCJHIA_42 (original name was: gnl|Prokka|OEMCJHIA_42); gnlProkkaOEMCJHIA_43 (original name was: gnl|Prokka|OEMCJHIA_43); gnlProkkaOEMCJHIA_44 (original name was: gnl|Prokka|OEMCJHIA_44); gnlProkkaOEMCJHIA_47 (original name was: gnl|Prokka|OEMCJHIA_47); gnlProkkaOEMCJHIA_49 (original name was: gnl|Prokka|OEMCJHIA_49); gnlProkkaOEMCJHIA_50 (original name was: gnl|Prokka|OEMCJHIA_50); gnlProkkaOEMCJHIA_51 (original name was: gnl|Prokka|OEMCJHIA_51); gnlProkkaOEMCJHIA_52 (original name was: gnl|Prokka|OEMCJHIA_52); gnlProkkaOEMCJHIA_53 (original name was: gnl|Prokka|OEMCJHIA_53); gnlProkkaOEMCJHIA_55 (original name was: gnl|Prokka|OEMCJHIA_55); gnlProkkaOEMCJHIA_56 (original name was: gnl|Prokka|OEMCJHIA_56); gnlProkkaOEMCJHIA_57 (original name was: gnl|Prokka|OEMCJHIA_57); gnlProkkaOEMCJHIA_60 (original name was: gnl|Prokka|OEMCJHIA_60); gnlProkkaOEMCJHIA_61 (original name was: gnl|Prokka|OEMCJHIA_61); gnlProkkaOEMCJHIA_62 (original name was: gnl|Prokka|OEMCJHIA_62); gnlProkkaOEMCJHIA_63 (original name was: gnl|Prokka|OEMCJHIA_63); gnlProkkaOEMCJHIA_66 (original name was: gnl|Prokka|OEMCJHIA_66); gnlProkkaOEMCJHIA_72 (original name was: gnl|Prokka|OEMCJHIA_72); gnlProkkaOEMCJHIA_73 (original name was: gnl|Prokka|OEMCJHIA_73); gnlProkkaOEMCJHIA_74 (original name was: gnl|Prokka|OEMCJHIA_74)

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gnlProkkaOEMCJHIA_8 - Region 1 - RRE-containing

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Location: 157,362 - 177,715 nt. (total: 20,354 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

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core biosynthetic genes

additional biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

gnlProkkaOEMCJHIA_15 - Region 1 - phosphonate

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Location: 484,045 - 498,309 nt. (total: 14,265 nt) Show pHMM detection rules used

phosphonate: (phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase or phosphonatesMeIsocitrateLyase))

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core biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

gnlProkkaOEMCJHIA_16 - Region 1 - phosphonate

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Location: 797 - 19,891 nt. (total: 19,095 nt) Show pHMM detection rules used

Region on contig edge.

phosphonate: (phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase or phosphonatesMeIsocitrateLyase))

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core biosynthetic genes

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resistance

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

gnlProkkaOEMCJHIA_18 - Region 1 - NRPS-like

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Location: 69,032 - 111,461 nt. (total: 42,430 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

No matches found.

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 69031 - 111461:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg18_90

AMP-binding (12..401): X

nrpys: Ile, Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DALFLGVVLK
Nearest Stachelhaus code(s):: Leu or Ile DAIYLGVVLK (59% 8Å match); aIle DALVFGVVLK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

gnlProkkaOEMCJHIA_59 - Region 1 - NRPS

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Location: 48,935 - 94,107 nt. (total: 45,173 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 48934 - 94107:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg50_73

AMP-binding (8..447): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DYFGSGSLIK
Nearest Stachelhaus code(s):: Ala DMGGFGCLIK (50% 8Å match); Leu DYFTFGLIIK (41% 8Å match); Hpr DYQYCGHLIK (32% 8Å match)
Stachelhaus code match:: 60% (weak)

AMP-binding (1210..1629): X

nrpys: Tyr, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMTNMGEVAK
Nearest Stachelhaus code(s):: Asn DMTKVGEVGK (56% 8Å match)
Stachelhaus code match:: 70% (weak)