LOCUS       gnlProkkaPNEKKDIO_85    5208 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PNEKKDIO_85.
ACCESSION   gnlProkkaPNEKKDIO_85
VERSION     gnlProkkaPNEKKDIO_85
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2025-04-28 02:47:33
            Original ID  :: gnl|Prokka|PNEKKDIO_85
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5208
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1419
                     /NRPS_PKS="Domain: PCP (169-236). E-value: 1.1e-22. Score:
                     71.8. Matches aSDomain: nrpspksdomains_ctg85_1_PCP.1"
                     /NRPS_PKS="Domain: Thioesterase (257-405). E-value:
                     4.5e-26. Score: 84.5. Matches aSDomain:
                     nrpspksdomains_ctg85_1_Thioesterase.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg85_1"
                     /sec_met_domain="AMP-binding (E-value: 3.8e-34, bitscore:
                     106.5, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 6.6e-14, bitscore:
                     41.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVAGDGVARGYLNLSDLTAEKFVDNPFAPGERMYRTGDLARWLP
                     DGNIEYLGRIDHQVKIRGYRIEIGEVETSLSNIGEVQEAIVIAREDENGDKALYAYFVA
                     SRPYHVSEMKEKLSDQLPNYMIPSYFIQLEKMPLTPNGKIDRKALPVPEGELQTGKEYV
                     APRTPIEAKLAEIWQEVLGLAQVGVKDNFFNIGGHSIKILKLIQYMNEKMDVEINFQTI
                     FYTPTIELMALQLMNTNPRLKDKTGFIPLNQNGEINVFCFPPPPGLGMIYLEMAKFLEN
                     ECIVHVTDFIDNYDDYDEILNEYVDQITSIQNEGPYILLGYSGGGNLAFEVTKTMNQRG
                     REVSDIIMLDTTPWNKKVQEIASKILAETNLTLVDAHELMATKYAENKRNKFLMYMENL
                     TNSGLVKANIHNLVVDTTTTSLKKKWITKTSKEYIEYRGVGTHDELLNHRYIQENAGTI
                     KQVLNEIKNKIFER"
     protocluster    1..5208
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:1419]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..1419
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..5208
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..5208
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /tool="antismash"
     CDS_motif       4..69
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg85_1_0001"
                     /evalue="7.40E-10"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg85_1"
                     /protein_end="23"
                     /protein_start="1"
                     /score="31.1"
                     /tool="antismash"
                     /translation="VVAGDGVARGYLNLSDLTAEKF"
     CDS_motif       151..216
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg85_1_0002"
                     /evalue="7.20E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg85_1"
                     /protein_end="72"
                     /protein_start="50"
                     /score="37.2"
                     /tool="antismash"
                     /translation="YLGRIDHQVKIRGYRIEIGEVE"
     aSDomain        508..708
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg85_1_PCP.1"
                     /evalue="1.10E-22"
                     /label="ctg85_1_PCP.1"
                     /locus_tag="ctg85_1"
                     /protein_end="236"
                     /protein_start="169"
                     /score="71.8"
                     /tool="antismash"
                     /translation="EAKLAEIWQEVLGLAQVGVKDNFFNIGGHSIKILKLIQYMNEKMD
                     VEINFQTIFYTPTIELMALQLM"
     aSModule        508..1215
                     /domains="nrpspksdomains_ctg85_1_PCP.1"
                     /domains="nrpspksdomains_ctg85_1_Thioesterase.1"
                     /final_module
                     /incomplete
                     /locus_tags="ctg85_1"
                     /tool="antismash"
                     /type="unknown"
     aSDomain        772..1215
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg85_1_Thioesterase.1"
                     /evalue="4.50E-26"
                     /label="ctg85_1_Thioesterase.1"
                     /locus_tag="ctg85_1"
                     /protein_end="405"
                     /protein_start="257"
                     /score="84.5"
                     /tool="antismash"
                     /translation="NVFCFPPPPGLGMIYLEMAKFLENECIVHVTDFIDNYDDYDEILN
                     EYVDQITSIQNEGPYILLGYSGGGNLAFEVTKTMNQRGREVSDIIMLDTTPWNKKVQEI
                     ASKILAETNLTLVDAHELMATKYAENKRNKFLMYMENLTNSGLV"
     CDS_motif       934..1002
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg85_1_0003"
                     /evalue="2.90E-09"
                     /label="NRPS-te1"
                     /locus_tag="ctg85_1"
                     /protein_end="334"
                     /protein_start="311"
                     /score="29.3"
                     /tool="antismash"
                     /translation="QNEGPYILLGYSGGGNLAFEVTK"
     CDS             1790..1966
                     /locus_tag="ctg85_2"
                     /transl_table=11
                     /translation="MLLKSWQLTFFILLYITNINLYDIELSLMKQQVTNKFNLLLIKTL
                     GLDQRMDIIKLKF"
     CDS             complement(2022..2849)
                     /locus_tag="ctg85_3"
                     /transl_table=11
                     /translation="MLKTYQKLLAMGIFLIVLCSGNAAFAATNQVGGLSNVGFFHDYLI
                     EPFSALLKGVAGLFHGEYGLSIILVTIIVRIVVLPLFVNQFKKQRIFQEKMAVIKPQID
                     SIQVKLKKTKDPEKQKELQMEMMKLYQEHNINPLAMGCLPMLIQSPIMIGLYYAIRSTP
                     EIASHSFLWFSLGQSDILMSLSAGIMYFVQAYIAQKLSAKYSAVPQNPAAQQSAKLMVF
                     IFPVMMTIFSLNVPAALPLYWFTSGLFLTVQNIVLQMTHHKSKKTAALTESVK"
     CDS             complement(2880..3161)
                     /locus_tag="ctg85_4"
                     /transl_table=11
                     /translation="MFVESINDVLFLVDFFTIILPALTAIGIAFLLRECRAGEQWKSKR
                     TDEHQTVFHINRTDFLIIIYHRITTWIRKVFRMNSPVNDEEDAGSLLL"
     CDS             3240..4484
                     /locus_tag="ctg85_5"
                     /transl_table=11
                     /translation="MTGKSRIIFHIDMNSFYASVEMAYDPALRGKPVAVAGNVKERKGI
                     VVTCSYEARARGVKTTMPVWQAKRHCPELIVLPPNFDRYRNSSRAMFTILREYTDLVEP
                     VSIDEGYMDMTDTPYSSRALETAKEIQSRLQKELLLPSSIGIAPNKFLAKMASDMKKPL
                     GITILRKRQVPDILWPLPVGEMHGVGKKTAEKLKGLGIHTIGELAAADEHSLKRLLGIN
                     GPRLKNKANGIHHAPVDPERIYEFKSVGNSSTLSHDSSDEEELLGVFRQLAASVSDRLQ
                     RKEVMASKLFIMIRYADWKTITRSTTLRNPIDQKNDILKEAEHLFFKHWNKNPVRLLGI
                     TGTDLVEKEQAYKQLDLFSFNEDAKDEPIQQMMEKLNKKYGTKLIRKGATLKKEESKTK
                     GTSFNKDFFQDEKKS"
     CDS             4594..5208
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1100:3-hydroxyisobutyrate dehydrogenase (Score: 270;
                     E-value: 4.2e-82)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg85_6"
                     /transl_table=11
                     /translation="MSKQQIGVIGLAVMGKNLALNIESRGFSVSVYNRSSSKTEEFLQE
                     AKGKNVVGTYSIEEFVQSLETPRKILLMVKAGTATDATIQSLLPHLEKDDILIDGGNTY
                     YKDTQRRNKELAESGIHFIGTGVSGGEEGALKGPSIMPGGQKEAHELVKPILEAISAKV
                     DGEPCTTYIGPDGAGHYVKMVHNGIEYGDMQLISESYFILKQ"
ORIGIN
        1 ttggtggtag ctggtgatgg agtggcacgt gggtacttaa acctctctga tttaacagcg
       61 gagaagttcg tagacaaccc atttgcccct ggagaacgga tgtatcgtac gggagatttg
      121 gctcgttggt tgccggatgg gaacattgag tacctgggac ggatcgatca tcaggtaaaa
      181 atacgtggct accgcattga aatcggggaa gtggaaacct cgttgtcaaa cataggagag
      241 gtacaggaag ccatcgtcat tgcacgtgaa gatgaaaatg gagacaaggc attatatgcc
      301 tattttgtag cgagtcgtcc atatcacgta agtgagatga aagaaaaact gtcggaccaa
      361 ttgcctaatt acatgattcc gtcttatttt atacagcttg agaaaatgcc gttaacaccg
      421 aatgggaaaa ttgaccgcaa ggctctcccg gtacctgaag gagaattaca gaccggaaaa
      481 gagtatgtgg ctccacggac tccaatcgaa gcaaaattag ctgaaatttg gcaagaggtg
      541 cttggcctag cacaggttgg agtaaaagat aatttcttta atataggcgg acattctatc
      601 aaaatactaa aattaattca atatatgaat gagaaaatgg atgttgagat aaactttcaa
      661 accatttttt atactcctac aattgaatta atggcattac aattaatgaa tacgaatcca
      721 aggttaaaag ataagacagg atttattccc ttaaatcaaa atggggaaat aaatgtgttc
      781 tgctttccac ctcctccagg tcttggaatg atatatttgg agatggctaa atttttggaa
      841 aatgagtgca ttgtgcatgt gacagatttt atagataact atgacgatta tgatgagata
      901 ctaaatgagt atgtagacca aattacgagt atacagaatg agggtccata tatacttttg
      961 gggtattctg gtggaggtaa tttagcattt gaagtgacta aaacaatgaa tcaaagaggg
     1021 agggaggtat ccgacattat catgttagat actacacctt ggaacaaaaa agttcaggaa
     1081 atagcatcta agatacttgc agagactaat ttaacacttg tagatgctca cgaattgatg
     1141 gctactaaat atgcagaaaa taaacgtaat aagtttctga tgtacatgga gaatttaacg
     1201 aactcaggat tagttaaagc taatatccat aaccttgttg tcgacacaac gacaacctca
     1261 cttaagaaaa agtggataac gaaaacatct aaagaatata ttgaatatag gggagtcgga
     1321 acccatgatg agctacttaa tcatagatat attcaagaaa atgcaggaac tatcaagcaa
     1381 gttttgaatg agattaaaaa taagattttc gagaggtgat ataattcttg tttaaatcat
     1441 ttgtttcctg tattgccaaa gtccaattat tggactttgg caataagttt ttttctaaag
     1501 cagagtgttt attcgaagct ttttatacgt gatcattgtt cttaagtcat atagattgaa
     1561 gaagtagtaa ttcagaagtt ttttattaat ggaaaagttt tcacttttta tgataaggga
     1621 aggatcaccc aactaataac cttcgttgat gctagagaat gaaatcgttt ttgtacatgt
     1681 aagacttaat aaactttctt ttacctaaat acttcaatca aaaaaggcta tgactaattg
     1741 aaatgtttag tggtgaaaga ttgtatggat cgaattgaag gaatcgtcca tgcttcttaa
     1801 aagctggcaa ttaacctttt tcattctctt atatataacc aacataaatc tttatgatat
     1861 agaattatct cttatgaagc aacaagtgac aaacaagttt aatttattgc ttattaaaac
     1921 gttaggacta gatcaaagaa tggatattat aaaactaaaa ttttagaagt tatgaaagga
     1981 tccaaaaaca cccgcgaccg taaagccggg ggtgtttcct tttatttcac cgactcagta
     2041 agagcggctg tttttttact tttatgatgc gtcatctgaa ggacgatgtt ttgcactgtc
     2101 aaaaacagtc cgcttgtaaa ccagtacagc ggaagggctg ccggcacatt aagcgagaaa
     2161 atcgtcatca tcacagggaa gatgaacacc atcaattttg cggattgctg cgccgcaggg
     2221 ttttgcggga cagcggaata tttcgcgctc agtttttgag cgatataggc ttggacaaaa
     2281 tacataatcc ctgcactaag ggacatgaga atatcagatt gccctaaact gaaccataga
     2341 aacgagtgtg acgcaatttc aggtgttgag cgaatcgcgt aataaagccc aatcataatc
     2401 ggagactgaa tcagcattgg aagacagccc atcgcaagcg ggttgatatt atgctcctga
     2461 tacagcttca tcatttccat ttgcagttct ttttgctttt ccgggtcctt cgtctttttc
     2521 aatttaacct ggatactgtc aatctgaggt ttaataacgg ccattttctc ttggaagatg
     2581 cgctgttttt tgaactgatt gacaaacaaa ggcaatacaa ctatacgcac aataatggtt
     2641 acgaggataa tagagagtcc gtattctccg tgaaacagcc cggcaacacc cttaagcaga
     2701 gcggaaaacg gttcgattaa ataatcgtgg aaaaaaccta cgttgctgag gcctcccacc
     2761 tgattagtcg ctgcaaatgc agcattgccc gagcataata cgatcaaaaa gatacccata
     2821 gccaaaagtt tttgatatgt ttttaacaat tttgttctcc tcctttttat aaatgcggtt
     2881 tataaaagaa gagaaccggc gtcttcctca tcgttcacag gcgaattcat gcggaagact
     2941 ttacgtatcc aagttgtaat gcgatgatat ataataataa gaaagtctgt tcggttaatg
     3001 tgaaagaccg tctgatgttc atctgttcgt tttgatttcc attgctcgcc cgcacggcac
     3061 tcccgtaaga gaaatgcaat cccgattgcc gttagagcag gaagaataat tgtgaaaaaa
     3121 tcgactaaga ataaaacgtc atttatcgat tccacaaaca ttgtcatcac atccattcaa
     3181 tagcttcatt ttactatatg tacaagctgt tgtctaatga ccgaaaagga gatttgttta
     3241 tgacgggaaa gagccgaatt attttccata ttgatatgaa tagcttttat gcgtcggttg
     3301 aaatggcata tgatccggct ctccggggaa agccggtcgc agttgccgga aacgtaaagg
     3361 agcgaaaggg cattgtggta acatgcagct acgaagcaag agcgcgcggc gtcaaaacaa
     3421 cgatgccggt ttggcaagcg aagcgccatt gtcctgagct catcgttctt cctccgaact
     3481 ttgaccgcta tagaaactct tcacgagcga tgtttaccat tcttcgtgaa tatactgacc
     3541 tggtggagcc tgtctccatc gacgaaggct atatggacat gaccgataca ccatacagca
     3601 gccgcgctct ggagacggca aaagaaatcc aaagcaggct gcaaaaagag ctgctgctcc
     3661 cgtcaagcat agggattgcg ccgaataaat ttctcgctaa gatggcgtct gatatgaaaa
     3721 aaccgcttgg aattacgatc ttaagaaaga ggcaagtgcc tgatatcctg tggccgcttc
     3781 ctgttggaga gatgcacgga gtcggcaaaa agacagcgga aaaactgaag ggactcggca
     3841 tccatacaat cggagagcta gctgcagctg atgaacactc gctgaagcgc ctgcttggca
     3901 tcaacggtcc gcgccttaaa aataaagcaa atggcattca ccatgcgccg gttgacccgg
     3961 agcgcattta cgaatttaaa agcgtcggca actcatcgac actttcccat gattccagtg
     4021 atgaagaaga gctgttaggc gtctttagac agcttgccgc ttctgtgagt gaccgtttac
     4081 agcgcaagga agtcatggct tcgaagcttt ttattatgat tagatatgcg gattggaaaa
     4141 ccatcacaag aagcactacg ctgagaaacc cgattgatca aaaaaatgac attttgaaag
     4201 aagcggagca cctctttttt aaacattgga ataaaaatcc tgtcaggctg cttggcatta
     4261 ccggaacgga tttagtagaa aaagagcagg cctataaaca gctcgatttg ttcagcttta
     4321 atgaagatgc gaaggatgag cccattcagc agatgatgga aaagttgaat aaaaagtacg
     4381 gtaccaaact gatcagaaaa ggggcaacgt taaaaaaaga ggagagcaaa acaaagggaa
     4441 caagctttaa taaagatttt tttcaagatg aaaagaaaag ctgaatcgct tgaaaaaaag
     4501 ggtgaatgta tggtaatttc tttattataa atatcatttt attttaattt aaattttaaa
     4561 atgattaaac gtttgcagaa gggacgttag aatatgtcaa aacaacaaat cggcgttatc
     4621 ggacttgcgg tcatgggtaa aaatctggct ttaaatatcg aaagccgggg attttctgtt
     4681 tctgtttaca acagatcaag cagtaaaact gaggagtttt tacaggaggc aaaaggaaaa
     4741 aatgttgttg gcacttacag cattgaagaa tttgtccaat ccttggaaac accgcgcaaa
     4801 atcttattaa tggttaaagc gggaactgca acagatgcga caattcaatc tcttcttcct
     4861 catctagaga aggatgatat tttaattgac ggcggtaata catactataa agatacacag
     4921 cgccgtaaca aagaactcgc tgaaagcggc attcatttta tcggaacagg cgtttccggc
     4981 ggagaagaag gggcgcttaa aggaccttct atcatgcctg gcggacaaaa agaagcacat
     5041 gagcttgtaa aaccgattct tgaagccatt tctgcaaaag ttgacggaga accttgtacg
     5101 acatatatcg gtccggacgg cgccggccat tacgtaaaaa tggtgcacaa cggaattgaa
     5161 tacggagata tgcagctgat ctctgaatca tatttcattc tgaaacag
//
