LOCUS       gnlProkkaKGFGMFNE_502   2353 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KGFGMFNE_502.
ACCESSION   gnlProkkaKGFGMFNE_502
VERSION     gnlProkkaKGFGMFNE_502
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 00:30:04
            Original ID  :: gnl|Prokka|KGFGMFNE_502
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2353
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1962
                     /NRPS_PKS="Domain: AMP-binding (2-228). E-value: 6.2e-45.
                     Score: 145.3. Matches aSDomain:
                     nrpspksdomains_ctg385_1_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (339-402). E-value: 1.1e-19. Score:
                     62.3. Matches aSDomain: nrpspksdomains_ctg385_1_PCP.1"
                     /NRPS_PKS="Domain: Thioesterase (425-641). E-value:
                     1.1e-22. Score: 73.5. Matches aSDomain:
                     nrpspksdomains_ctg385_1_Thioesterase.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     141.6; E-value: 4.7e-43)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg385_1"
                     /sec_met_domain="AMP-binding (E-value: 1.5e-63, bitscore:
                     201.8, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.1e-12, bitscore:
                     33.7, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRFALITSYSFDMTVTSNWLPFIAGASLHILSDNATKDIEKLLYF
                     IDEEKINFLNVTPSHFSMLVNTLGFLERPVNLSPNMTIMLGAEIINVSDINRWLENYPL
                     HKFINEYGPTETTVASTFYPIPIEEDGKCHLNIVPIGKPIYNTQVYVLNDNLEYTLPEV
                     PGILYIGGEGVSCGYLNKEEKTKSVFINNPITHEGIVYNTGDVVKMTVTGDIVFVGRKD
                     FQVNVRGYRIELGEIENALLKVSGITEACAEIQYDVNKQPVVVAFYVTANNCDLEYKDI
                     MSVLQMKIPHYMLPSAMARIEQIPISANGKTDKKQLPNIANQKQNFVKRDIVGPRNERE
                     KQITLIWEKVLGIPEVGVFDNFWDIGGDSIRSVRLIKELKEVGLTNIKLKDLFDKPTIA
                     ELLEQEIENNKVENLICMKKVSTPYAKLICLPYAAGTPGMYSTFSGDFTGEINLYTVQY
                     PGHGDGREIKSSVEEVGALLANELKEADKEIPLFIMGYSYSCYIAYDICKRFEQENIPI
                     QGIIMIGGTPPTLRDDLMQFFSNDDNALLDYSRAKDLLNEELIATLSDEEKHEYLHELR
                     MNTVAMVNYKFLDCKLKTPLCSIVGREDEPTIRNNQHLWNNYFQKVSFHQLPGGHVLIT
                     KYHAELAKLIMNYIELHV"
     protocluster    1..2353
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:1962]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..1962
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..2353
                     /SMILES="NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..2353
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /tool="antismash"
     aSDomain        7..684
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg385_1_AMP-binding.1"
                     /evalue="6.20E-45"
                     /label="ctg385_1_AMP-binding.1"
                     /locus_tag="ctg385_1"
                     /protein_end="228"
                     /protein_start="2"
                     /score="145.3"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FALITSYSFDMTVTSNWLPFIAGASLHILSDNATKDIEKLLYFID
                     EEKINFLNVTPSHFSMLVNTLGFLERPVNLSPNMTIMLGAEIINVSDINRWLENYPLHK
                     FINEYGPTETTVASTFYPIPIEEDGKCHLNIVPIGKPIYNTQVYVLNDNLEYTLPEVPG
                     ILYIGGEGVSCGYLNKEEKTKSVFINNPITHEGIVYNTGDVVKMTVTGDIVFVGRKDFQ
                     VNVR"
     aSModule        7..1923
                     /complete
                     /domains="nrpspksdomains_ctg385_1_AMP-binding.1"
                     /domains="nrpspksdomains_ctg385_1_PCP.1"
                     /domains="nrpspksdomains_ctg385_1_Thioesterase.1"
                     /final_module
                     /locus_tags="ctg385_1"
                     /monomer_pairings="X -> X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       328..357
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg385_1_0001"
                     /evalue="3.50E-03"
                     /label="NRPS-A_a5"
                     /locus_tag="ctg385_1"
                     /protein_end="119"
                     /protein_start="109"
                     /score="10.0"
                     /tool="antismash"
                     /translation="EYGPTETTVA"
     CDS_motif       484..567
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg385_1_0002"
                     /evalue="1.70E-09"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg385_1"
                     /protein_end="189"
                     /protein_start="161"
                     /score="29.9"
                     /tool="antismash"
                     /translation="EVPGILYIGGEGVSCGYLNKEEKTKSVF"
     CDS_motif       649..714
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg385_1_0003"
                     /evalue="8.80E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg385_1"
                     /protein_end="238"
                     /protein_start="216"
                     /score="36.9"
                     /tool="antismash"
                     /translation="FVGRKDFQVNVRGYRIELGEIE"
     CDS_motif       736..753
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg385_1_0004"
                     /evalue="9.90E+01"
                     /label="NRPS-A_a5"
                     /locus_tag="ctg385_1"
                     /protein_end="251"
                     /protein_start="245"
                     /score="-3.0"
                     /tool="antismash"
                     /translation="GITEAC"
     aSDomain        1018..1206
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg385_1_PCP.1"
                     /evalue="1.10E-19"
                     /label="ctg385_1_PCP.1"
                     /locus_tag="ctg385_1"
                     /protein_end="402"
                     /protein_start="339"
                     /score="62.3"
                     /tool="antismash"
                     /translation="EKQITLIWEKVLGIPEVGVFDNFWDIGGDSIRSVRLIKELKEVGL
                     TNIKLKDLFDKPTIAELL"
     aSDomain        1276..1923
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg385_1_Thioesterase.1"
                     /evalue="1.10E-22"
                     /label="ctg385_1_Thioesterase.1"
                     /locus_tag="ctg385_1"
                     /protein_end="641"
                     /protein_start="425"
                     /score="73.5"
                     /tool="antismash"
                     /translation="KLICLPYAAGTPGMYSTFSGDFTGEINLYTVQYPGHGDGREIKSS
                     VEEVGALLANELKEADKEIPLFIMGYSYSCYIAYDICKRFEQENIPIQGIIMIGGTPPT
                     LRDDLMQFFSNDDNALLDYSRAKDLLNEELIATLSDEEKHEYLHELRMNTVAMVNYKFL
                     DCKLKTPLCSIVGREDEPTIRNNQHLWNNYFQKVSFHQLPGGHVLITKYHAEL"
     CDS             1965..2351
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1060:4'-phosphopantetheinyl transferase (Score: 110.8;
                     E-value: 1.1e-33)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg385_2"
                     /transl_table=11
                     /translation="MLVLPFSDIAVEIVEDSPFATSDNLFNEEKIFIKREWSEIRLKTY
                     ILGRCAAHRALQTLQKEIVPILKGRDGEPIWPFDIVGSISHKDAIAVAAVGKKKRYKGI
                     GVDIEDQTTILSKKEYSLFCTPQEI"
ORIGIN
        1 aacaggtttg ctttaataac atcgtattcc tttgatatga ctgttacttc taattggcta
       61 cctttcattg ccggagcttc tttacacatt ctttccgata atgcaactaa agatatagag
      121 aaacttctgt attttataga tgaagaaaaa atcaattttt tgaatgtaac tccctctcat
      181 ttttcaatgt tggtaaatac gttgggattt ttggagagac ctgtaaacct atcacccaat
      241 atgactatta tgctgggtgc tgaaattatc aatgtttcgg atataaacag gtggttagaa
      301 aattatcctt tgcacaaatt tattaatgaa tacggcccta cagaaacaac ggtggcttcc
      361 actttttatc ctatcccgat tgaagaagac ggcaaatgtc atttaaatat agttccaata
      421 gggaaaccga tttataatac acaagtatat gtattaaatg ataatttaga atatacacta
      481 ccagaagttc ctggtatcct ttatattgga ggcgagggag tgtcatgtgg ttatttgaat
      541 aaagaagaga aaacgaaatc tgtatttatc aataatccta ttactcatga aggaattgta
      601 tataatacag gagacgttgt aaagatgact gtaactggcg atattgtatt tgtaggacgt
      661 aaagattttc aagtaaatgt cagagggtat agaattgaat taggagaaat agagaatgct
      721 ttattaaaag tatcaggtat tacagaggct tgtgcagaga ttcaatatga tgtaaacaaa
      781 cagcctgtag ttgtagcttt ttatgttaca gcaaataatt gtgatttaga atacaaggat
      841 ataatgtctg ttctgcaaat gaagatacct cactatatgt taccttccgc aatggctaga
      901 atagagcaga ttccaatttc tgccaatgga aaaacagaca agaagcaatt acccaatatt
      961 gcgaatcaga aacaaaattt tgttaaaagg gatattgtag gtccaaggaa tgaaagggaa
     1021 aaacaaataa ccttaatctg ggaaaaggta ttagggatac cagaggtagg tgtatttgat
     1081 aatttctggg atataggagg cgactctatc cgatctgtaa ggttaataaa agaattaaag
     1141 gaggtcggat taaccaacat caaattaaaa gatttattcg ataaacctac gatagccgaa
     1201 ctgcttgaac aagaaataga gaataataaa gttgagaatc tcatttgtat gaaaaaggtt
     1261 tctactccat atgcaaaact tatatgtctt ccatatgcag caggaacccc tggtatgtat
     1321 agtacatttt ctggggattt taccggagaa atcaacttat atacagtaca atatccggga
     1381 catggagatg gacgtgagat aaagtcctct gtagaagaag ttggtgcgct attagccaat
     1441 gaactgaaag aggctgataa agagattcct ttgtttatca tgggatattc ttacagttgc
     1501 tatattgctt atgatatctg taaacgattt gaacaagaaa atattccgat acagggtatt
     1561 ataatgattg gtggaactcc tcctacattg cgtgatgact taatgcaatt cttttcaaat
     1621 gacgataatg cccttttgga ttattcacgt gcaaaagatc tgttgaatga agaattgatc
     1681 gcaactttat cagatgaaga aaaacatgaa tatttgcatg agttgagaat gaatactgta
     1741 gcaatggtaa attacaagtt tcttgattgt aaattgaaaa ccccattatg ttccatcgtc
     1801 ggtagagagg atgagcctac aattcgaaac aatcaacatc tatggaataa ttactttcag
     1861 aaagtttctt tccaccaatt gccaggaggg catgttctta ttacaaaata tcatgcggaa
     1921 ttagctaagt tgataatgaa ttatatagaa ttgcatgtat aaatatgctt gtattaccct
     1981 tttccgatat agcagtagaa attgtggaag atagtccttt tgctacttca gataatctct
     2041 ttaatgagga aaaaatattc attaaaagag aatggtctga aatacgtctt aaaacttata
     2101 ttttgggacg ttgtgccgca catcgtgcct tacagacttt acaaaaggaa atagttccca
     2161 ttttaaaagg aagagatggt gaaccaatat ggccatttga catagttggt tctatatctc
     2221 ataaagatgc aatagcagtt gctgctgttg gaaagaaaaa gaggtataag ggtattggag
     2281 tagatattga agatcaaacg acgatattat caaaaaagga atattctctt ttttgtacac
     2341 cccaagagat agc
//
