LOCUS       c00091_gnlPro..         5897 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GLJBLKLI_133.
ACCESSION   c00091_gnlPro..
VERSION     c00091_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-03 17:59:11
            Original ID  :: gnl|Prokka|GLJBLKLI_133
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5897
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..414)
                     /locus_tag="ctg91_1"
                     /transl_table=11
                     /translation="MAKVQIKSEKLTPFGGIFSIMEQFDALLAQTIDSSLGLRCTMFGY
                     QYSEILRSLMCVYLCGGSCIEDVTTHLMKHLSLHPTLRTCSADTILRAIEELTCKNITY
                     KSASGKSYDFNTADKMNCLLVNALLATGQLKSGQ"
     protocluster    1..5897
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[665:3641]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      666..3641
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5897
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5897
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(666..1400)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg91_2"
                     /sec_met_domain="Radical_SAM (E-value: 6.4e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             complement(1401..3641)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg91_3"
                     /sec_met_domain="Gly_radical (E-value: 2.7e-46, bitscore:
                     145.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 3.5e-282, bitscore:
                     925.5, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENEYWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMVAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFERMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKMTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREMQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             3859..5577
                     /locus_tag="ctg91_4"
                     /transl_table=11
                     /translation="MRNILFTVLFIGILSLIASPISLASKSLHKANMINLSNNAIICMH
                     QDPDGYLWIGTYDGLNLYNGKDTYVYRFELNNKNSLCSNIIHKISDAEPGFLWISTSLG
                     INKFSLKKRKVTESYPGYMESDLVATDSSGITLAICKENRISCYTPFSDGFRDLPREGI
                     GPQTVRTLFAGTDGYFYLLLTSGILKKLTPDFSNTTFNLLSEERQFHNKPILSAFHENN
                     HIFIVDTDNKLYRQQVDGSGKEFLFDLSGMTNKYGNIIKICTFQSNVYIVFRNGNILDL
                     SQPENTIDTGIGIFCLMNDKRQEILWVGTDGQGIRMFYDKPDLFGSILLKDLPINIQNP
                     IRSLYTDNDRSLWFGTKGDGIVRIQAYDTYPNKKAIPQSAITHFTTADGLSSNRVYSFQ
                     RSEFHPCIWIGTEGPGLCYYSYKENRMKTIPQQEDTPPIRYIHSICEVNDSTLWLATTG
                     NGLLKVTLRMDKVTPTISKIQTFPLKNGENTCKEIQSMIYDNDSTLFLGSRGGYGVISF
                     NIFNQKYEFLQTNNLRNPAIGDVLSVCQTEDSTFYAGASSGLTRIKFLKSATYRV"
     CDS             complement(5718..5897)
                     /locus_tag="ctg91_5"
                     /transl_table=11
                     /translation="IMQRLKTREFGLRATSRIKTFVFKFISVPAKWIKTSRRHVLNIYS
                     DNNTYANLFKTDFG"
ORIGIN
        1 ttgaccggat ttcaattgac cagtagcaag cagggcatta accagtaagc agttcatctt
       61 gtctgcagta ttgaaatcat aggatttgcc agaagcagat ttataggtga tgttcttaca
      121 agtcagttct tcgatagcac gtaatatggt gtctgcgctg caagtgcgaa gagttggatg
      181 aagagacaaa tgtttcatca agtgagttgt aacatcctca atacatgagc caccacaaag
      241 atatacgcac atcagagagc gtagtatctc gctatattga taaccaaaca tagtgcatct
      301 caatcccaag gaggaatcta tggtttgagc taaaagagca tcaaattgct ccataataga
      361 aaaaattccc ccaaaaggag tgagtttctc ggattttatt tgtacttttg ccatgtcatt
      421 cagaattttg tttgttttta ttttgcaaca ctaagataag tgaaatttct gacatggcaa
      481 aatcctgagc aactttttgt tgctcaggtg cttataaaaa acattaaatt atagtgttgc
      541 ggaaataagg attaaatagt caaggttagt ctaggcaaaa aattattact atcgtttaaa
      601 agctaatcgg attagattgt tttccctgat tagctgttgc ataatcgcaa ggcttgatcg
      661 agagatcagg ccttgttatt ttctggtttg tagcgagaga atatcgcttt cgcattatct
      721 aaacgttctt ttgataaggg ttccaccccc tttaacggat aatcgaggcc taattgctca
      781 tatttatagg ttcccatcgt atgataaggc aataattcca ctttgcgaac cactttatat
      841 gaactgacat accgagctaa cgcctccagc cactcgtcat tatccgtata tccggggaca
      901 ataacatggc gaatccaagt atctatgcca cgtctctcca attcatccaa gaacagaagc
      961 gtattatcct gcttgacacc cgccagtaac ggatgtaaat ccgggtttaa ggttttgatg
     1021 tccaataaca ctaagtccgc ataatccaag acttcccaag cttttgaggt acatacgaaa
     1081 ccagatgtgt ccaaagctgt gtgaagacct tgctcttggc acaaacggaa aaactctttc
     1141 agaaactcag gttgcaataa aggttcccca ccggtaacag tcaccccccc acgggcgata
     1201 aaactcttat accgtaaaac ttccgtcaat agctcgccgg gagtcatctg atatttcacc
     1261 ttccctttcg ggttccaagt atccgggtta tggcaataca ggcaacgcaa cgggcagccc
     1321 tgcataaaaa cgacaaagcg aattccggga ccatctacgg tcccgaaact ctctaatgaa
     1381 tgtatcttac cttttatcac ttacagtgaa tggttaatgg ttcttgaaat aacatccaac
     1441 tgttgttcac gagtcagttt cacgaagttt accgcatatc cggatacacg gatcgtaagt
     1501 tgcggatatt tctcgggatg ttccatagcg tcgatcaaca aatccttatc aaatacgtta
     1561 acattcagat gctgacctcc atccggagta aaatagccat ccaataagct aaccagatta
     1621 ttgatctgca tttcacgctc ctttcccaac gtagccggag aaacagcgaa agtataagag
     1681 atgccgtcgt gcgcatgctg gaatggaagc ttcgctaccg aagctagcgc agcgatagca
     1741 cctttcgtat cacgaccgtt catagggttc gcgcccggag cgaaaggagt tccaccgata
     1801 cggccatccg gtgtcgtacc agtcttctta ccatatacca cattagaggt gatcgtaagg
     1861 atggactgcg taggtttcgc atggcgatag gtctcgtgct gacgcaaata ttccatgaac
     1921 ttctcggtga tctctacagc gatactatcc gtacgatcat cgttattacc gaaaggaaca
     1981 tattcgccct cacgttcgaa atcaaccgcc aagccacgct cgtcacggat cacacgtact
     2041 ttcgtatcac ggatagcagc caacgaatcg gcaacgatag aaagaccggc gatacctgtc
     2101 gcacggatac gctctacatc cccatcgtga agcgacatct cgaatgcctc atacgcgtat
     2161 ttatcatgca tataatggat tatcttcaag gcgttcacat acactttcgc caaccagcgc
     2221 atcatttgct catacttagc catcacctca tcgtattcca gatactcgcc gcgaaccggc
     2281 tcgaacatcg gggctacctg tacgccgctg cgctcatccc gtcctccgtt gatcgcatat
     2341 aataaacatt tcgccaaatt cgcacgggct ccgaagaact gcatctgctt accgatcttc
     2401 ataggagaaa cgcaacaagc gataccataa tcatctccat aatccggacg catcaaatcg
     2461 tcgttctcat attggatagc cgacgtatcg atagagacct tggcacagaa acgtttccag
     2521 ttctcgggag cgttcttaaa ccacagaacc gtcaaattgg gttctggtgc cggacctaga
     2581 ttatataatg tatggagata acgataggaa gttcttgtta ccaaagaacg gccatccact
     2641 ccttggccgc ccaaggactc tgttacccaa accggatcac cggagaacaa atcattgtat
     2701 tccggcgtac gtaagaaacg aacgatacgc aacttgatga tcagctggtc tactaactct
     2761 tgcgcctcct cttccgtcat cttaccctca cggatgtctt tctcgatata aatatccaaa
     2821 aacgtagaag tacgtccgat agacatcgcc gcgccatctt ggtcttttac cgccgccaga
     2881 taagctaagt acacaaactg aaccgcctca cgagcgtttt gcgccgggcg ggtcacgtcg
     2941 aagccataac tagcgcacat tctctcgaac gctttcaacg ccttgatttg ctcagaaatc
     3001 tcctcacggc tctggataat ctcttccgtg atctcttgga tatccaaacg attcaagaaa
     3061 cgctttttct cctcgatcaa gattgccgta ccatataaag cgacacgacg atagtcaccg
     3121 ataatacgtc ctctaccata cgcatcgggc aagccggtga tgatagccga acgacgtgcg
     3181 gcaaccatct cttccgtata tgcggagaaa acgccctcgt tatgcgtctt acggtacttc
     3241 gtgaagatct ctttcgtcat cgggtctaaa tgataaccgt aggcttccaa gctattctcg
     3301 accatacgga taccaccctt cgggaagata ccacgtttca gcggagcatc ggtttgtaac
     3361 ccaacgataa cctcattctc cttatcaata tagcccggac catatacatc gatcccttga
     3421 ggtaattttg tctcggcgtc ataaactccc ttttctctct cgaccttaaa catctcggtc
     3481 agcttgttcc agaccttacg ggtcttctcg gaagaagcga ctaaaaagga atcatcccct
     3541 tcataaggcg tatagttact ctggataaaa tctcttacat tgatctctct cttccaggtc
     3601 tcacccttaa agtgatccca gtactcattc tcaaatttca taaacaatct tatctaatac
     3661 cacattttta ccggcacaaa ggtaaaactt ttatcctcat gttcctgtaa catttgcgac
     3721 acccctctaa aatccctata aataggtatt aaaaatatac aagaagaaac gggatagtaa
     3781 tgcgatgctt tgcacgaagt ctccatttta ttcactatct ttgcctacag aacgtaaaag
     3841 ttaatctgga tcaaacacat gagaaatatc ctgtttaccg ttttatttat aggaatacta
     3901 tctttaatcg catctcctat ctcactcgcg tctaaatctt tgcataaggc aaatatgatt
     3961 aatttgtcga acaatgccat tatttgcatg catcaagatc cggatggcta tttgtggatc
     4021 gggacttacg acggacttaa cttatacaac ggaaaagata cgtatgtata tcgtttcgaa
     4081 ttaaataaca aaaactctct ttgtagtaat atcattcaca aaatatccga cgcagaaccc
     4141 ggctttttat ggatatcaac ctctttaggt atcaataaat tctcgctaaa gaaacggaaa
     4201 gtcaccgagt cttacccggg atacatggag agtgatctgg tagcgaccga ttcatccggt
     4261 atcacattag ccatttgtaa agaaaaccgg atatcttgtt acaccccatt tagcgatgga
     4321 ttccgggatc tgccgagaga agggataggc cctcaaaccg tacggacttt attcgccgga
     4381 acagacggat atttttattt actattgacc tcgggaatac tgaaaaagtt aacaccggat
     4441 ttctccaata caacttttaa tttactatcg gaagaacgcc aattccataa taaaccgatc
     4501 ttatccgcat ttcatgagaa taaccatatc ttcattgtcg ataccgacaa taagttatat
     4561 cgccaacagg tagacggaag cgggaaagaa ttcctgtttg atctgtctgg aatgacaaat
     4621 aaatatggaa acatcattaa gatatgtacc ttccaatcca atgtatatat cgttttccgt
     4681 aacggcaata ttctagacct aagccaaccg gaaaacacga tagacacggg catcggtatc
     4741 ttttgcctga tgaacgataa aagacaggag attctttggg tagggaccga tggacaaggc
     4801 atacggatgt tttacgataa acccgatctt ttcggctcta tcttactcaa ggatttaccg
     4861 attaatatac agaaccccat acgttcttta tatacagaca atgatcgatc cctttggttc
     4921 gggacaaaag gagacggaat tgtccggata caagcctatg acacttaccc aaacaaaaag
     4981 gcgattccac aaagcgctat cacgcatttc accaccgccg acggattatc cagcaaccgg
     5041 gtatatagct tccagagaag cgaattccat ccttgtatct ggatcggtac ggaaggcccg
     5101 gggctctgtt attattctta taaagaaaat cgaatgaaga cgatcccgca gcaagaggac
     5161 actcccccta tccgctatat ccattctatt tgtgaggtaa acgactcgac attatggttg
     5221 gcgacaaccg gcaacggatt actgaaggtt actttacgta tggataaagt cacgcctaca
     5281 atcagtaaga tacagacctt cccgctaaaa aacggggaga atacctgcaa agagattcaa
     5341 tccatgatat atgataacga ctcgacctta tttctcggga gtcgtggcgg ttatggggtt
     5401 attagtttta acatattcaa ccagaaatat gagttcctgc aaaccaacaa tttaaggaat
     5461 ccggccattg gagacgtgtt atctgtatgc cagaccgagg attctacttt ctatgcaggc
     5521 gccagctccg gacttacccg gattaaattc ctcaaatccg caacctatag ggtttagtgg
     5581 gattttggtt gcaaagcgac aaaattcatt ttattgtaca tatttcttgc acaacagaaa
     5641 tgtcgcagac acaaaatccc cttaccccat aaagcgactt gaggtaatac gctggtttaa
     5701 ccagaaaagc atggtcttta accaaagtct gtcttgaaca ggttggcata agtattgttg
     5761 tctgagtaaa tgttcaatac atgcctgcgt gatgtcttaa tccattttgc aggaacagag
     5821 atgaacttga aaacaaaggt cttgatcctg ctggtggcac gcaatccaaa ttcacgggtt
     5881 ttcaatctct gcataat
//
