LOCUS       c00027_gnlPro..         5861 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HNAHMGIL_33.
ACCESSION   c00027_gnlPro..
VERSION     c00027_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-03 17:49:12
            Original ID  :: gnl|Prokka|HNAHMGIL_33
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5861
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5861
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2398:5374]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2399..5374
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5861
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5861
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..322
                     /locus_tag="ctg27_1"
                     /transl_table=11
                     /translation="YNLRGGKERIFDDMNNGFGWNRLPKSFMAQNTVFLLMTALIRNFY
                     KAIMQRLKTREFGLRATSRIKTFVFKFISVPAKWIKTSRRHVLNIYSDNNTYANLFKTD
                     FG"
     CDS             complement(463..2181)
                     /locus_tag="ctg27_2"
                     /transl_table=11
                     /translation="MRNILFTVLFIGILSLIASPISLASKSLHKANMINLSNNAIICMH
                     QDPDGYLWIGTYDGLNLYNGKDTYVYRFELNNKNSLCSNIIHKISDAEPGFLWISTSLG
                     INKFSLKKRKVTESYPGYMESDLVATDSSGITLAICKENRISCYTPFSDGFRDLPREGI
                     GPQTVRTLFAGTDGYFYLLLTSGILKKLTPDFSNTTFNLLSEERQFHNKPILSAFHENN
                     HIFIVDTDNKLYRQQVDGSGKEFLFDLSGMTNKYGNIIKICTFQSNVYIVFRNGNILDL
                     SQPENTIDTGIGIFCLMNDKRQEILWVGTDGQGIRMFYDKPDLFGSILLKDLPINIQNP
                     IRSLYTDNDRSLWFGTKGDGIVRIQAYDTYPNKKAIPQSAITHFTTADGLSSNRVYSFQ
                     RSEFHPCIWIGTEGPGLCYYSYKENRMKTIPQQEDTPPIRYIHSICEVNDSTLWLATTG
                     NGLLKVTLRMDKVTPTISKIQTFPLKNGENTCKEIQSMIYDNDSTLFLGSRGGYGVISF
                     NIFNQKYEFLQTNNLRNPAIGDVLSVCQTEDSTFYAGASSGLTRIKFLKSATYRV"
     CDS             2399..4639
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg27_3"
                     /sec_met_domain="Gly_radical (E-value: 2.7e-46, bitscore:
                     145.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 3.5e-282, bitscore:
                     925.5, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENEYWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMVAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFERMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKMTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREMQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             4640..5374
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg27_4"
                     /sec_met_domain="Radical_SAM (E-value: 6.4e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             5626..5859
                     /locus_tag="ctg27_5"
                     /transl_table=11
                     /translation="MAKVQIKSEKLTPFGGIFSIMEQFDALLAQTIDSSLGLRCTMFGY
                     QYSEILRSLMCVYLCGGSCIEDVTTHLMKHLSL"
ORIGIN
        1 ctacaatctt cgtggtggca aggaacgcat cttcgatgac atgaacaatg gctttggctg
       61 gaatcgattg ccaaaatcgt tcatggcaca gaatactgta ttcctgctta tgacagctct
      121 catcagaaac ttctacaaag ctattatgca gagattgaaa acccgtgaat ttggattgcg
      181 tgccaccagc aggatcaaga cctttgtttt caagttcatc tctgttcctg caaaatggat
      241 taagacatca cgcaggcatg tattgaacat ttactcagac aacaatactt atgccaacct
      301 gttcaagaca gactttggtt aaagaccatg cttttctggt taaaccagcg tattacctca
      361 agtcgcttta tggggtaagg ggattttgtg tctgcgacat ttctgttgtg caagaaatat
      421 gtacaataaa atgaattttg tcgctttgca accaaaatcc cactaaaccc tataggttgc
      481 ggatttgagg aatttaatcc gggtaagtcc ggagctggcg cctgcataga aagtagaatc
      541 ctcggtctgg catacagata acacgtctcc aatggccgga ttccttaaat tgttggtttg
      601 caggaactca tatttctggt tgaatatgtt aaaactaata accccataac cgccacgact
      661 cccgagaaat aaggtcgagt cgttatcata tatcatggat tgaatctctt tgcaggtatt
      721 ctccccgttt tttagcggga aggtctgtat cttactgatt gtaggcgtga ctttatccat
      781 acgtaaagta accttcagta atccgttgcc ggttgtcgcc aaccataatg tcgagtcgtt
      841 tacctcacaa atagaatgga tatagcggat agggggagtg tcctcttgct gcgggatcgt
      901 cttcattcga ttttctttat aagaataata acagagcccc gggccttccg taccgatcca
      961 gatacaagga tggaattcgc ttctctggaa gctatatacc cggttgctgg ataatccgtc
     1021 ggcggtggtg aaatgcgtga tagcgctttg tggaatcgcc tttttgtttg ggtaagtgtc
     1081 ataggcttgt atccggacaa ttccgtctcc ttttgtcccg aaccaaaggg atcgatcatt
     1141 gtctgtatat aaagaacgta tggggttctg tatattaatc ggtaaatcct tgagtaagat
     1201 agagccgaaa agatcgggtt tatcgtaaaa catccgtatg ccttgtccat cggtccctac
     1261 ccaaagaatc tcctgtcttt tatcgttcat caggcaaaag ataccgatgc ccgtgtctat
     1321 cgtgttttcc ggttggctta ggtctagaat attgccgtta cggaaaacga tatatacatt
     1381 ggattggaag gtacatatct taatgatgtt tccatattta tttgtcattc cagacagatc
     1441 aaacaggaat tctttcccgc ttccgtctac ctgttggcga tataacttat tgtcggtatc
     1501 gacaatgaag atatggttat tctcatgaaa tgcggataag atcggtttat tatggaattg
     1561 gcgttcttcc gatagtaaat taaaagttgt attggagaaa tccggtgtta actttttcag
     1621 tattcccgag gtcaatagta aataaaaata tccgtctgtt ccggcgaata aagtccgtac
     1681 ggtttgaggg cctatccctt ctctcggcag atcccggaat ccatcgctaa atggggtgta
     1741 acaagatatc cggttttctt tacaaatggc taatgtgata ccggatgaat cggtcgctac
     1801 cagatcactc tccatgtatc ccgggtaaga ctcggtgact ttccgtttct ttagcgagaa
     1861 tttattgata cctaaagagg ttgatatcca taaaaagccg ggttctgcgt cggatatttt
     1921 gtgaatgata ttactacaaa gagagttttt gttatttaat tcgaaacgat atacatacgt
     1981 atcttttccg ttgtataagt taagtccgtc gtaagtcccg atccacaaat agccatccgg
     2041 atcttgatgc atgcaaataa tggcattgtt cgacaaatta atcatatttg ccttatgcaa
     2101 agatttagac gcgagtgaga taggagatgc gattaaagat agtattccta taaataaaac
     2161 ggtaaacagg atatttctca tgtgtttgat ccagattaac ttttacgttc tgtaggcaaa
     2221 gatagtgaat aaaatggaga cttcgtgcaa agcatcgcat tactatcccg tttcttcttg
     2281 tatattttta atacctattt atagggattt tagaggggtg tcgcaaatgt tacaggaaca
     2341 tgaggataaa agttttacct ttgtgccggt aaaaatgtgg tattagataa gattgtttat
     2401 gaaatttgag aatgagtact gggatcactt taagggtgag acctggaaga gagagatcaa
     2461 tgtaagagat tttatccaga gtaactatac gccttatgaa ggggatgatt cctttttagt
     2521 cgcttcttcc gagaagaccc gtaaggtctg gaacaagctg accgagatgt ttaaggtcga
     2581 gagagaaaag ggagtttatg acgccgagac aaaattacct caagggatcg atgtatatgg
     2641 tccgggctat attgataagg agaatgaggt tatcgttggg ttacaaaccg atgctccgct
     2701 gaaacgtggt atcttcccga agggtggtat ccgtatggtc gagaatagct tggaagccta
     2761 cggttatcat ttagacccga tgacgaaaga gatcttcacg aagtaccgta agacgcataa
     2821 cgagggcgtt ttctccgcat atacggaaga gatggttgcc gcacgtcgtt cggctatcat
     2881 caccggcttg cccgatgcgt atggtagagg acgtattatc ggtgactatc gtcgtgtcgc
     2941 tttatatggt acggcaatct tgatcgagga gaaaaagcgt ttcttgaatc gtttggatat
     3001 ccaagagatc acggaagaga ttatccagag ccgtgaggag atttctgagc aaatcaaggc
     3061 gttgaaagcg ttcgagagaa tgtgcgctag ttatggcttc gacgtgaccc gcccggcgca
     3121 aaacgctcgt gaggcggttc agtttgtgta cttagcttat ctggcggcgg taaaagacca
     3181 agatggcgcg gcgatgtcta tcggacgtac ttctacgttt ttggatattt atatcgagaa
     3241 agacatccgt gagggtaaga tgacggaaga ggaggcgcaa gagttagtag accagctgat
     3301 catcaagttg cgtatcgttc gtttcttacg tacgccggaa tacaatgatt tgttctccgg
     3361 tgatccggtt tgggtaacag agtccttggg cggccaagga gtggatggcc gttctttggt
     3421 aacaagaact tcctatcgtt atctccatac attatataat ctaggtccgg caccagaacc
     3481 caatttgacg gttctgtggt ttaagaacgc tcccgagaac tggaaacgtt tctgtgccaa
     3541 ggtctctatc gatacgtcgg ctatccaata tgagaacgac gatttgatgc gtccggatta
     3601 tggagatgat tatggtatcg cttgttgcgt ttctcctatg aagatcggta agcagatgca
     3661 gttcttcgga gcccgtgcga atttggcgaa atgtttatta tatgcgatca acggaggacg
     3721 ggatgagcgc agcggcgtac aggtagcccc gatgttcgag ccggttcgcg gcgagtatct
     3781 ggaatacgat gaggtgatgg ctaagtatga gcaaatgatg cgctggttgg cgaaagtgta
     3841 tgtgaacgcc ttgaagataa tccattatat gcatgataaa tacgcgtatg aggcattcga
     3901 gatgtcgctt cacgatgggg atgtagagcg tatccgtgcg acaggtatcg ccggtctttc
     3961 tatcgttgcc gattcgttgg ctgctatccg tgatacgaaa gtacgtgtga tccgtgacga
     4021 gcgtggcttg gcggttgatt tcgaacgtga gggcgaatat gttcctttcg gtaataacga
     4081 tgatcgtacg gatagtatcg ctgtagagat caccgagaag ttcatggaat atttgcgtca
     4141 gcacgagacc tatcgccatg cgaaacctac gcagtccatc cttacgatca cctctaatgt
     4201 ggtatatggt aagaagactg gtacgacacc ggatggccgt atcggtggaa ctcctttcgc
     4261 tccgggcgcg aaccctatga acggtcgtga tacgaaaggt gctatcgctg cgctagcttc
     4321 ggtagcgaag cttccattcc agcatgcgca cgacggcatc tcttatactt tcgctgtttc
     4381 tccggctacg ttgggaaagg agcgtgaaat gcagatcaat aatctggtta gcttattgga
     4441 tggctatttt actccggatg gaggtcagca tctgaatgtt aacgtatttg ataaggattt
     4501 gttgatcgac gctatggaac atcccgagaa atatccgcaa cttacgatcc gtgtatccgg
     4561 atatgcggta aacttcgtga aactgactcg tgaacaacag ttggatgtta tttcaagaac
     4621 cattaaccat tcactgtaag tgataaaagg taagatacat tcattagaga gtttcgggac
     4681 cgtagatggt cccggaattc gctttgtcgt ttttatgcag ggctgcccgt tgcgttgcct
     4741 gtattgccat aacccggata cttggaaccc gaaagggaag gtgaaatatc agatgactcc
     4801 cggcgagcta ttgacggaag ttttacggta taagagtttt atcgcccgtg ggggggtgac
     4861 tgttaccggt ggggaacctt tattgcaacc tgagtttctg aaagagtttt tccgtttgtg
     4921 ccaagagcaa ggtcttcaca cagctttgga cacatctggt ttcgtatgta cctcaaaagc
     4981 ttgggaagtc ttggattatg cggacttagt gttattggac atcaaaacct taaacccgga
     5041 tttacatccg ttactggcgg gtgtcaagca ggataatacg cttctgttct tggatgaatt
     5101 ggagagacgt ggcatagata cttggattcg ccatgttatt gtccccggat atacggataa
     5161 tgacgagtgg ctggaggcgt tagctcggta tgtcagttca tataaagtgg ttcgcaaagt
     5221 ggaattattg ccttatcata cgatgggaac ctataaatat gagcaattag gcctcgatta
     5281 tccgttaaag ggggtggaac ccttatcaaa agaacgttta gataatgcga aagcgatatt
     5341 ctctcgctac aaaccagaaa ataacaaggc ctgatctctc gatcaagcct tgcgattatg
     5401 caacagctaa tcagggaaaa caatctaatc cgattagctt ttaaacgata gtaataattt
     5461 tttgcctaga ctaaccttga ctatttaatc cttatttccg caacactata atttaatgtt
     5521 ttttataagc acctgagcaa caaaaagttg ctcaggattt tgccatgtca gaaatttcac
     5581 ttatcttagt gttgcaaaat aaaaacaaac aaaattctga atgacatggc aaaagtacaa
     5641 ataaaatccg agaaactcac tccttttggg ggaatttttt ctattatgga gcaatttgat
     5701 gctcttttag ctcaaaccat agattcctcc ttgggattga gatgcactat gtttggttat
     5761 caatatagcg agatactacg ctctctgatg tgcgtatatc tttgtggtgg ctcatgtatt
     5821 gaggatgtta caactcactt gatgaaacat ttgtctcttc a
//
