LOCUS       c00575_gnlPro..         6003 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GEDPCNNE_577.
ACCESSION   c00575_gnlPro..
VERSION     c00575_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 22:05:24
            Original ID  :: gnl|Prokka|GEDPCNNE_577
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6003
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6003
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3066:6001]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3067..6001
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6003
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6003
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..1397)
                     /locus_tag="ctg575_1"
                     /transl_table=11
                     /translation="MKHFTIFQGFYYAIAEMTEEEIVSTIGSFTYREKVEEIRRIFAEQ
                     GEKAANEKKKELPAIAFSASYRGRRTKVNLVKYLGHIVIDIDHLSKEELARILPIIKRC
                     NYTRIAFISPKGMGVKIIVRACHPDETLPETLQEIEDFHHAAYTRLVSFYTELCQIEID
                     TSGQDVARTCLFSYDPEIYFNPNADAFLVDQPQASYKVSNRKNLSGSKQQTPPDGPPTN
                     EDTALNAHSANASLVLTLTYYHNKSEKYIAGNRNNYLHHLSCTFNRYGIPQEEASAFIK
                     SQFTDFPADETVSLINSAYAHTDEFNTCKLNGTQKRILRIEQYISEHYETRYNEVLHIM
                     EYRRRHPDTEKPEPFRILDEMMENSIWIEINELGYPCTVKTIENLIYSDFSQSYHPIRE
                     YFELLPEWDGTDYIRILADSVQTSHQEFWAECLERYLVAMCAAATQENIVNHTVLLLCS
                     EIQNIGK"
     CDS             complement(1484..1714)
                     /locus_tag="ctg575_2"
                     /transl_table=11
                     /translation="MNYKEDSFLIRTYTKAELAHLYNPHVCLKVALQILRRWIIYNLPL
                     LHELEQEGYRARNRLLSPKQVATIIHFLGEP"
     CDS             1908..2411
                     /locus_tag="ctg575_3"
                     /transl_table=11
                     /translation="MAMTVSYSVVPRKNPAKKSEPAKYYAQAQASGELDFEELCEAITS
                     RSTCTETDVRAAISGILYEVKRALKAGRIARLGDLGSLQIGLNSEGAASVKEFSGSMIK
                     GAHLIFRPGKTLAELMKILSYQQVQTRAVVQAGAGDGGGEDPDKYPDSGGDGSGDEEAP
                     DPTV"
     CDS             2471..2638
                     /locus_tag="ctg575_4"
                     /transl_table=11
                     /translation="MKEIVSRILDVIMYLIPFFGKRKRDRVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             2625..3074
                     /locus_tag="ctg575_5"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDEDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(3067..3795)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.5;
                     E-value: 1.4e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg575_6"
                     /sec_met_domain="Radical_SAM (E-value: 1.3e-29, bitscore:
                     92.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGIHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(3809..6001)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg575_7"
                     /sec_met_domain="Gly_radical (E-value: 4.3e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1173.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="SSEINVRDFVSHNITPYYGDASFLEGPTERTKAVWNRCLEALAEE
                     RENNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKPFGGINVVSKACH
                     ENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPDNYARGRIIGDYR
                     RMALYGIDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKDMKVMGEYYGLDLSR
                     PAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSKGTITESFAQELI
                     DQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKTSFRFLQTLYNLG
                     PSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQSDDYGIACCVSYQ
                     EIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTLKFEEVMDNYKKV
                     LTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGLSIALDSLSAIKY
                     AKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEELKKLPVYKNARPT
                     LSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDS
                     QDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLNRDMLYDAMEHPE
                     NYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 gtctttccta tgttctgtat ctcgctgcat agcagaagta ccgtgtgatt gacaatattc
       61 tcctgagtag ccgctgcgca catcgccacc agataacgtt ccaggcattc cgcccaaaac
      121 tcctgatgac tcgtctggac actatccgcc aggatacgga tatagtcggt tccgtcccat
      181 tcgggcagta attcaaagta ctcgcgaatg ggatgatacg actggctgaa atcggagtaa
      241 atcagatttt cgattgtttt gacagtgcag ggatatccaa gttcgttgat ttctatccag
      301 atactgtttt ccatcatctc atccaagatg cggaaaggct ccggtttctc ggtgtcggga
      361 tgccttctgc gatattccat gatatgcagc acttcgttgt agcgtgtttc gtagtgctcg
      421 ctgatatatt gctctatccg taatattcgt ttctgcgtac cattcagttt acaggtatta
      481 aattcgtcgg tatgagcgta ggcgctattg atcaaagaga ctgtctcgtc cgccggaaag
      541 tctgtaaact ggcttttgat aaaggcggat gcttcctcct gcggaatgcc gtagcgattg
      601 aaggtacatg agagatggtg cagataattg ttccggttgc ccgctatgta tttttcggac
      661 ttgttgtgat agtaggttaa agtcagcacc agagaagcgt tggcggaatg ggcgttgagc
      721 gctgtgtctt cattcgtcgg ggggccgtcc ggaggagtct gttgtttact gccggagaga
      781 tttttacgat ttgaaacctt gtaggatgcc tgaggttgat ctacaagaaa agcgtcagcg
      841 ttcggattga aataaatctc cggatcgtaa gagaacagac aagtgcgtgc cacatcctgg
      901 ccggaggtgt cgatttctat ctgacagagt tcggtgtaaa aagaaacgag tctggtgtaa
      961 gctgcatgat ggaagtcttc tatttcctga agggtttcgg gcaatgtctc atctggatgg
     1021 catgcgcgta ctatgatttt cacgcccatc cctttgggac tgataaaggc gatgcgggtg
     1081 taattgcatc tcttgataat agggaggatg cgtgccaatt cttctttgct gaggtggtcg
     1141 atgtctatca cgatgtgtcc cagatatttg acgaggttca ctttggtacg ccttcctcgg
     1201 tagctggcgg agaaggcgat tgccggcaat tcttttttct tctcattggc agccttttct
     1261 ccttgctctg caaagatgcg gcgtatttct tcgacttttt cccggtaggt aaaactgccg
     1321 atagtggaca ctatttcttc ttcggtcatc tctgctattg cgtagtagaa accttgaaat
     1381 atggtgaaat gtttcatttt tattttgatt attgtttgtc gatgcaaagg aacttctttg
     1441 cggaaatgtg taaaggaact ttgtataaaa tggcaataag ttactacggt tcgcccaaaa
     1501 agtgtatgat agttgccact tgtttcggcg aaaggagacg gttgcgtgcg cggtagcctt
     1561 cctgttcgag ttcgtgcagg agagggaggt tgtaaataat ccaacgacga agtatttgta
     1621 aagcaacttt gaggcagaca tggggattgt aaaggtgagc cagttccgct ttggtatagg
     1681 tgcggataag gaaagaatct tctttgtaat tcatgatgtt ttcttcttgt tttatgctat
     1741 aaagttacat aaaattcctt gtttttcaaa ggtttgactt cgtttttatt cgttgggaat
     1801 caagaaactt cgttggggaa tatggcttgt ccggataccc ttatctttgt gctgttaaca
     1861 agaaggggat gcatcctatg tattataaac tttaaaatta ttttattatg gcaatgacag
     1921 tatcttattc agtggtgccg cgcaagaatc ctgcaaaaaa gagcgaaccg gcaaagtatt
     1981 atgcacaggc gcaggcgtcg ggtgagctgg actttgaaga gttgtgcgaa gcgatcacca
     2041 gtcgttctac ttgtacggaa acggatgtac gtgcggctat ttcgggtatt ctttacgaag
     2101 tgaaacgtgc gttgaaggca ggaagaattg cgagactggg cgatttgggc agtttgcaga
     2161 taggactcaa cagtgaaggt gcggcatcgg tcaaggaatt ttccggttca atgattaagg
     2221 gggcgcatct tattttccgc cccggtaaga cgctggcgga gttgatgaag attctgagtt
     2281 atcagcaagt gcagactcgt gcggtggtgc aggcgggtgc aggcgatggc gggggagaag
     2341 accctgacaa gtatccggat tccggtggtg atggctccgg tgatgaagag gctccggacc
     2401 ctacggtata attggcagac gggtcaaggt aataacgaat tagaataact tccattaata
     2461 ttttgataag atgaaagaaa tagtaagtcg gattttagat gtgatcatgt atctgattcc
     2521 tttcttcgga aagagaaagc gggacagggt ggtgagagaa gtgcgctatc atactacttg
     2581 taaggaggtg tgcaaagtga aaacgacgga aagggaaaag gaacatgaga aagattagtc
     2641 tgatcgtgat ccattgttcc gccacccgtg tcgattgcga ctttacggca aaggatgtgg
     2701 atacggctca ccgttatcgt ggcttttcgt gctgggggta tcattattat atacgcaagt
     2761 cgggcgagat tgagccgatg cgggatgaag atacggtcgg agctcatgca cgtggctata
     2821 atgcgatcag cttaggtgta tgttatgagg gcggtctgga tgagaatgga aaagctgcgg
     2881 atacgcgtac cccccgtcag aaggaggcgc tacatcgctt ggtacacgaa ttgttgcagc
     2941 gttatccgga agctaaagta gtagggcatc gggatttaag tcccgacacc aactataatg
     3001 ggatcgtaga tccttgggaa cggattaaag aatgtccttg ctttgaggtc attggagaat
     3061 tctccattaa ttaactacca ccgtagtgaa atactctttg aatacctccg cagctttttc
     3121 aagctgttcg ggggtatttt cttttacccc tttcatcttg tattcctgtc ccatcgcttc
     3181 gtacttgtgt atgcccagtg tatggtaagg caatatttcc acccgttgga tcatcttgta
     3241 ttttccgagt gcttctccca gtgcgcgtat gtcttcttca aaatcgctgt atccgggcac
     3301 tagcacgtag cgtagccaga aaggtttctc ctgttcttcc agccaggcgg ccgtgcggat
     3361 ggtttgctcg ttgcttcttc cggtgagcgt ctgatggcgg ttagggttga attcctttat
     3421 atccaataac acaagatcgg tcagtctgaa taattcttct acgtcttcgt tccaaagtcc
     3481 tccgttgctg tccagacaga catggatgcc tttttctttc agttcgcgga ccagcgggac
     3541 gagtgctttc gcctgaaacg tcggttcgcc tccggagaaa gtgattcccc ctcgtttccc
     3601 gaagaagggg cgctggctca ttgccatgcg gacgatttct tccggtgggg taggcgtgcc
     3661 tccctttcct gctatcgtat ccggattggc gcaatagagg cagcggaagt tgcatccttg
     3721 aagaaaaacg acgagccgta agcccggccc gtcgaatgtt cccatacttt cgtatgaatg
     3781 tacgtttatc atcatataga gtaatagatt acatacgttc gtggaagcta cggctgataa
     3841 cttccagttg gtgttcgcgg ctcagcttca cgaagtttac ggcatagccg gaaacgcgga
     3901 tagtcagctg cggatagttt tccggatgct ccatggcatc gtacagcata tcacggttca
     3961 gtacgtttac gttcaggtgg tgggcacctt tggtgaagta accgtccatc atcgttacga
     4021 ggttctcgat acggtcttcg tccgtagcac ccagtgattt cggaacgata gagaaggtgt
     4081 tgctgatacc gtcctgtgag tcacggtaac gaagtttcgc tacagaactc agagaagcga
     4141 tcgcaccatt cttgtcgcgt ccgtgcatcg ggttggcacc cggagcgaag gcaacacctt
     4201 tggcacgtcc gtcgggagta gcaccggtct ttttgccgta catcacattg gaagtgatag
     4261 tcagcaagga gagggtagga cgggcattct tgtaaactgg caatttcttc aattcttcgc
     4321 tgaagaagta taccaggtct acgccaaggt ggtctacctt gtcgttgtcg ttaccgaagc
     4381 aagggaattc tccttcgatg tcgaagcctt ctgtcagacc gatatcattg cggcgggcag
     4441 ttactttcgc atatttgatg gcagacagtg aatcaagggc gatggaaagt ccggctacac
     4501 cgtaagccag gttgatgcgc gggttcgtat ctacaagagc catctgggcc ttctcgtagt
     4561 aatacttgtc gtgcatatag tggatgatgt tcatcgcttc gttatatacg cgggcgattt
     4621 cggtcagcac ttttttgtag ttgtccatta cttcttcgaa cttcagcgtg tcgctggtca
     4681 gcacggggat gttcttcacc atgactgtac cggtattctc gcaacgtccg ccgttgatgg
     4741 caagcaacag ggctttggcc aggttgcaac gggcgccgaa gaactgaatc tgcttgccta
     4801 tttcctggta tgatacgcag caggcgattc cgtagtcgtc ggactgacgt acttcacgca
     4861 tcaggtcgtc attttcgtac tggatagaag aagtgtcgat agaaaccttt gcacagaatt
     4921 ctttgaatcc ttccggaagt tccgggctcc agagtacggt caggttcggt tccggtgaag
     4981 ggccgaggtt gtacaatgtc tgcaggaaac ggaaggaggt ctttgtcact ttggtacgtc
     5041 cgtcgttgag acgtccgccc agagattcgg ttacccaggt cgggtcgcct gcaaagatgt
     5101 cgttgtatga ttgcatacgc aggtggcgga ccatacgcag tttgatgaca aactggtcga
     5161 tcagctcttg cgcgaatgat tcggtgatgg ttcctttgct cagttcatat tccagataga
     5221 tatcgaggaa agaagaaacg ttacccagtg acatagcggc accgtcttgt tctttgacgg
     5281 cggcaaggta agccatatat acccactgta cggcttcttg tgccgtgtaa gcgggacggc
     5341 tcaggtcgag accgtaatat tcgcccatca ctttcatgtc tttcagtgcc ttgatctgtt
     5401 ctgccacttc ttcgcgcagg cggatgcggg cctctgtcat cggaccggtg aggttgtgca
     5461 aatcttcctt ctttgcttcg atcagacggt cgatgccgta aagagccata cggcggtagt
     5521 caccgatgat gcgtccgcgt gcataattgt cgggaagtcc ggtaaggaat cccagcgaac
     5581 ggaaggaacg gatttcttcc gtatatacgt cgaatactcc gtcgttgtgc gtcttgcggt
     5641 agtgagtgaa aatatctttt acgcggtcgt ccacttccac gccgttttcg tgacaggctt
     5701 tgctgactac attgatacct ccgaaaggct tgatggcacg tttcagaagt tcgtctgtct
     5761 gcaggccgac gatcagttcg ttttccttgt cgatatatcc ggccttgtgg gaagtgatgg
     5821 ttgatacggt aacattatct aatgagcgga caccgttgtt ttctctttct tctgccagtg
     5881 cttcgaggca gcggttccat acggctttgg tacgttctgt aggtccttcg aggaatgaag
     5941 catctccgta atacggagtg atgttatgac ttacgaaatc tctgacgttg atttcgctgc
     6001 tcc
//
