LOCUS       c00177_gnlPro..         6897 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EGHNOKDK_177.
ACCESSION   c00177_gnlPro..
VERSION     c00177_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 21:48:03
            Original ID  :: gnl|Prokka|EGHNOKDK_177
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6897
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6897
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[79:3035]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      80..3035
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6897
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6897
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             80..2308
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg177_1"
                     /sec_met_domain="Gly_radical (E-value: 2.2e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1170.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKIDETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2310..3035
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg177_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.1e-31, bitscore:
                     98.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHERPFWLRYV
                     LVPGYSDSEEDIRLLGENLGKYRQIQRVEILPYHRLGVHKYEVMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(3104..5308)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 84.6; E-value: 1.3e-26)"
                     /gene_kind="other"
                     /locus_tag="ctg177_3"
                     /sec_met_domain="Fn3-like (E-value: 8.2e-28, bitscore:
                     84.6, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3 (E-value: 1.4e-75, bitscore:
                     242.6, seeds: 11, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 2.7e-53,
                     bitscore: 169.0, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKIIILMVCLWGLGNTFTSAQTTEKEKFISSLMKQMTLDEKIGQ
                     LAQCTYRGNFTGPDGGNIPKEEYVRQGRIGSMLNVIGVKDIRRYQELALQSRLRIPLIF
                     GLDVIHGYRTGFPLPLAEAASFDLAMIEEAARYNALESAADGLNWVFAPMVDISWDARW
                     GRVMEGAGEDPYYGSRVAEARVRGLQGDDLADTTTVMACMKHFAGYGAPIAGKEYNSVD
                     MSMGHFANFYMPPYKAAIKVGAATVMSAFNDFNNIPCTANDFLLNRLLREQWGFKGFVV
                     SDYNSVEELVNHRYAADKKDAAAKALNAGLDMEMVSTCYLTYLKELVQEGKVKESVLDD
                     AVRRILEKKYELGLFEDPFRYCNPERAARILNAPEVRNASLRMAERSVVLLENRESVLP
                     LTAQTRKIALIGGLSKSQYDMAGAWAATTDRSKVVTLYDALVRKGLEVNYAEGYDMKSN
                     QLTGLQEALQAAEASDVIVVAVGERFGQSGEKASKVNIAIPEGQQKLVSELAKTGKPVI
                     ALVMCGRPVIFNEIREHADAVLCTWWLGSEAGNAICNVLWGEYNPSAKLPMTFPAHVGQ
                     IPIYYQNKSTGRPTALKQTYKASYIDASTEPAYPFGYGLSYTDFGYSDLKLLPGKAAGV
                     HTEVSVKVTNTGKCAGEEVVQLYVQDKVASVTRPIKELKGFRKIHLNVGESKTVAFQIT
                     DDALGFYDNEMNYMVEPGDFVFMVGGSSDDVQQITYNLKE"
     CDS             complement(5339..6895)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 79.4; E-value: 5.5e-25)"
                     /gene_kind="other"
                     /locus_tag="ctg177_4"
                     /sec_met_domain="Fn3-like (E-value: 3.6e-26, bitscore:
                     79.4, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 4.2e-52,
                     bitscore: 165.2, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LIAFGALDTPREDKSIDKDNAQSKEIALDLAREGVVLLKNDNGTL
                     PYRNGRTLVIGPNADIIPTGGGSGFVTPYSVVSLYDGMVQLKGGRQIELLSDSLLYKDM
                     SQQIYTDDSFTQNGFKSEYYNTRNLTGELFQAVIEPAIDYAWKYGAPFDGMPVDQFSAR
                     WTGVYKADKDGTVKFQLAGDDGYRLFVNDKLITGDWGNHSFSTRSAFMQVSAGEIYRLR
                     IEYFDNVGEATVSFQAGMMDEERLASSLKHARNVIVCAGFNSSTEGEGFDRPFALSYGQ
                     EYLINKVASLHDNVAVVVNAGGGIDFRNWGQSVQAILMAWYPGQEGGKALAEIITGKLS
                     PSGKLPVSIEEKWEDNPVYGNYYDNRNVPHKRVQYAEGVFVGYRGYDRNGKQPLYPFGY
                     GLSYSSFEYSNLSVEKTGGSRVTVSFDIKNTGKMDAAEAAQVYVRDVECSVPRPLKELK
                     GYDKVYLKKGETKRVRILLDEEAFAYYDVESHRFVVEKGAFEILAGPSSADLPLKATVV
                     L"
ORIGIN
        1 taaaattacg gaatttattc agtatttttg cgtgctctaa aggataacat tatctaacct
       61 actaacaaaa acaaatttta tggaattgaa caaaaccttt accaatgggc tttggaataa
      121 agaaatcaat gtcagtgatt tcgtcagtaa aaacatcgca ccctataccg gggatgcatc
      181 tttcttgcag ggacctaccg aacgtaccaa acgtatctgg gacatctgtc tgaaggctct
      241 cgaagaagaa aggaacaaca atggcgtccg ttcactggac aataccaccg tatctaccat
      301 cacttctcat aaagccgggt atattgataa ggacaatgaa cttattgtcg gtttgcagac
      361 tgatgaactg ttgaaacgat ccatcaaacc tttcggaggc atcaacgtgg tagcaaaagc
      421 atgccgtgaa aacggagtgg aagtggacga taaagtaaaa gacatcttca cccattaccg
      481 caaaacacat aacgacggcg tattcgatgt atataccgaa gaaatccgtt ccttccgctc
      541 actgggattc cttaccggat tgcccgacaa ctatgcccgc ggacgtatca tcggcgacta
      601 ccgccgattg gctctctacg gcatcgaccg tctgattgaa gccaagcagg aggacttgcg
      661 caacctcacc ggcccgatga ccgaagcacg catccgtcta cgcgaagaag tggcagaaca
      721 aatcaaagct ttgaaggaca tcaaggttat gggcgaatat tacggactcg acctcagcca
      781 tccggctaca tcagctcaag aggccgtgca atgggtatac atggcatatc ttgctgccat
      841 caaagagcag gacggagcag ccatgtcact gggtaacgta tcttcgttcc tcgacatttt
      901 catcgaatac gatttggctc acggcaagat tgacgagacg tttgcacagg aactgataga
      961 ccagttcgtc atcaaactac gtatggtacg ccatctgcgc atgcagtcgt acaatgatat
     1021 ctttgccggc gacccgactt gggtgaccga agccatcggc ggacgcttca acgacggacg
     1081 cgtgaaggtc accaagactt ctttccgctt tttgcagacg ctatacaacc tcggtccttc
     1141 tccggaaccg aacttgaccg tattgtggag tcccgaactg ccggaaggct tcaaggattt
     1201 ctgcgccaaa gtttctgtag ataccagctc catccagtac gaaaacgaca acctgatgcg
     1261 cgaagtgcgc aactgcgacg attacggcat tgcctgctgt gtatcctacc aagccatcgg
     1321 caagcaaatc cagttcttcg gcgcacgtgc caaccttgcc aaggccctgc tgcttgccat
     1381 caacggtggc cgttgcgaaa acaccggaac tgtcatggtg aaaggtattc ccgtattgac
     1441 cggcgagaca ctgaagtttg aggaagttat ggccaactac aagaaggtac tgactgaaat
     1501 agcccgtgtc tacaacgagg ccatgaatat catccactac atgcatgata agtattatta
     1561 tgagaaagcc cagatggcat tcatcgatac cgacccacgc atcaatctgg catacggtgt
     1621 tgccggcctt tccatcgcca tagactcact gtccgccatc aaatatgcca aagtcactgc
     1681 acgccgcaac gaaatcggcc tgaccgaagg tttcgacacc gaaggctcat tcccctgctt
     1741 cggcaataat gacgaccgtg tagaccacct gggagtagac ctcgtttact atttcagcga
     1801 agaactgaaa aagctgcctg tctacaagaa tgcccgtccc accctttcct tgctgactat
     1861 cacctccaac gtgatgtacg gcaagaagac cggtgccact ccggacggac gcgccaaagg
     1921 tgttgccttt gctccgggtg ccaacccaat gcacggacgt gacaagaacg gtgccatcgc
     1981 ttccttgagc tccgtagcca aattgcgcta ccgcgactcg caggacggca tcagcaatac
     2041 gttctccatt gttcccaaat cactgggccc gacaccggaa gaacgtgtag agaacctggt
     2101 aaccatgatg gacggctact tcaccaaagg tgcccaccat ttgaacgtaa acgtactgaa
     2161 ccgcgaaatg ctggaagatg ccatggaaca tcctgaaaag tatccgcaac tcaccatccg
     2221 cgtttccggc tatgccgtga acttcgtgaa gttgagccgc gagcatcagt tggaagtcat
     2281 cagccgttca ttccacgaaa gaatgtaata tgataaatgt acactcctac gaaagtatgg
     2341 gaacattcga cgggccgggt ctccggctcg tcgttttcct gcaaggatgt ccgttccgtt
     2401 gtctgtattg tgccaatccg gatacgattg atgtaaaagg aggcactccc accactccgg
     2461 aagagattct gcaaatggct atcagccaaa aggcattctt cgggaagaaa ggaggtatta
     2521 ctttctccgg cggtgagcct accctgcaag cagaagcgct gattcctctt ttcaaggagc
     2581 tgaaggcgaa cggcatccat atctgtctgg acagtaacgg aggcatctgg aatgaaaagg
     2641 tggaagagct ctttagcctg actgacctcg tgctgctgga catcaagcaa ttcaatcccg
     2701 aacgccaccg tacgctgacg ggacggagca acgaacagac actccgcacc gctgcctggc
     2761 tcgaagaaca tgagcgcccc ttctggctgc gttatgtact ggttcccggt tacagcgatt
     2821 cggaagaaga catccgcctg ctgggtgaaa atctcggcaa ataccggcag atacaacggg
     2881 tggaaatact tccttaccac cgtctgggcg ttcacaaata cgaagtcatg ggatgggatt
     2941 acaaattgaa agaagtgaag gaaaatactc cggaacaact ggaacgggca gaaaaactgt
     3001 tcaaagagta tttcacgaat gtgataacca actaaaacag aaaagcaagg ctgtccggat
     3061 atgattccag acagccttgc ttttcattta taggacaaga atcctattct ttcaagttat
     3121 aggtaatctg ctgtacatca tcactactgc cacccaccat aaagacgaaa tcaccaggtt
     3181 ctaccatata attcatctca ttgtcataga atcccagtgc atcgtcagtt atctgaaaag
     3241 ctacagtttt gctttcaccc acattcagat gaattttcct gaagcccttc aattccttta
     3301 tcggacgcgt aacagaggct accttatcct gcacatacaa ttgaactact tcctctcctg
     3361 cacacttacc ggtattagtc acttttaccg acacctccgt atggacgcct gcagccttgc
     3421 ccggcagcag tttcaaatca gaatatccaa aatccgtgta cgaaagccca tatccgaaag
     3481 gataggcagg ctcagtggaa gcatcgatat aagatgcctt ataggtctgt ttcaatgcgg
     3541 tagggcggcc ggtgctctta ttctgataat aaatgggaat ctgtcctacg tgtgcaggaa
     3601 atgtcatggg caatttagcc gaaggattat actctcccca caacacattg catatggcat
     3661 tgccggcctc acttcccagc caccaagtac acagtaccgc atcagcatgt tcccttatct
     3721 cattgaagat taccggacgc ccacacatga caagagcaat gacaggtttt ccagtctttg
     3781 ccagttccga caccagcttc tgctgcccct cgggtatggc tatattcact ttggaagcct
     3841 tctcgccact ctgaccgaat ctctcgccta ccgccacaac aatgacatcc gaagcctcag
     3901 cagcctgcaa tgcttcctgc agtccggtca attgattgct cttcatatca tagccttcag
     3961 catagttcac ctccagtcct ttccggacca gtgcatcata caaggtcacc actttgctcc
     4021 ggtccgtagt ggcggcccaa gcgcctgcca tatcgtattg ggattttgag agtcctccta
     4081 tcaaggctat cttcctcgtc tgcgccgtca acggaagtac ggattcccta ttctccagca
     4141 atacaacgga acgctcggcc atacggagcg aagcattgcg gacttcgggt gcattgagta
     4201 ttctggctgc tctttccgga ttacagtagc gaaaagggtc ctcaaacaat cccaactcat
     4261 atttcttctc caaaatacgc cgtactgcat catcaagcac actctccttt actttccctt
     4321 cttgaacaag ctccttcaga taagtcaagt agcaggtact taccatctcc atgtccagac
     4381 cagcattcag cgctttggct gcggcatctt tcttgtcagc agcataacgg tggttcacca
     4441 attcctctac cgaattataa tcggacacaa caaatccctt aaatccccac tgctctctca
     4501 atagccggtt gagcaagaaa tcattggcag tacaaggaat attattaaaa tcattgaaag
     4561 cactcatcac ggtagctgct cctaccttta tggccgcctt atagggaggc atataaaaat
     4621 tggcaaagtg gcccattgac atatccacac tattatactc ctttcctgct atcggtgcac
     4681 catatccggc aaaatgtttc atacatgcca tgacagtcgt cgtgtctgcc aaatcgtctc
     4741 cttgcaagcc tctgacacgc gcctccgcta cccgagaacc ataataaggg tcctcaccag
     4801 ccccttccat gacgcgtccc caacgtgcgt cccacgaaat atccaccatc ggggcaaaaa
     4861 cccagttcaa accatcggcg gcactttcca aagcattgta gcgtgcagct tcctcaatca
     4921 tggccaaatc gaaacttgca gcctcggcaa gcggaagcgg aaatccggta cgatagccgt
     4981 ggataacatc cagtccgaaa attaaaggaa tgcgcaaacg cgattgcaaa gcaagctctt
     5041 gataacggcg tatatccttt actcctatca cattcagcat agagcctatt ctgccttgcc
     5101 ggacatactc ttctttagga atatttccac cgtcgggtcc ggtaaaattc cctcggtagg
     5161 tgcattgtgc aagctggcct atcttttcat ccagcgtcat ctgcttcatc aagcttgaga
     5221 tgaatttctc cttctccgta gtttgtgccg aggtaaaggt gttaccaagt ccccacagac
     5281 aaaccatcag tattattatc tttttcatac tgccattctt gttttgatat ttattttatc
     5341 ataaaacaac agttgccttc aatggtaaat cagctgaaga aggacctgcc agaatctcaa
     5401 aagccccctt ttccactaca aaccgatgtg attccacatc atagtaggca aaggcttcct
     5461 catccagcaa tatccgaacg cgcttggttt cacccttctt caagtaaacc ttatcgtagc
     5521 ctttcaactc cttcaatgga cgcggaacag aacattccac atcccgcaca tatacctgag
     5581 ccgcttccgc agcatccatc tttccggtat tcttgatgtc aaagcttacc gtaacccggc
     5641 ttccccccgt cttctcaaca ctcagattac tatattcaaa actactgtat gacagtccat
     5701 acccgaaagg atagagcggc tgctttccgt tccggtcata tcccctataa cctacgaaaa
     5761 caccttcggc atactgcacc cgtttatgag gtacattgcg attgtcataa taattaccat
     5821 ataccggatt atcttcccat ttttcctcga tgctgactgg tagtttgcca cttggcgaaa
     5881 gcttgcccgt aatgatttcg gcaagggcct ttccaccctc ctgccccgga taccatgcca
     5941 tcaaaatagc ttgtacactc tgcccccaat tgcggaaatc tattccacct ccggcattca
     6001 ccaccacagc cacattatca tgaagtgaag ccactttatt aatgagatat tcctgcccgt
     6061 atgacaatgc aaacggacgg tcgaaccctt caccttctgt actgctgttg aacccggcac
     6121 atacaataac attcctggca tgcttcagtg aagaggccaa acgctcctca tccatcattc
     6181 ctgcctggaa actgacagtc gcctcaccca cattatcaaa atattcaata cgcaaccggt
     6241 atatctcccc tgcactgacc tgcataaatg cactgcgggt actgaatgaa tggttccccc
     6301 aatctccggt aatcagctta tcgtttacaa acagacggta tccgtcatcg ccagccagct
     6361 ggaacttgac agttccatcc ttgtcagcct tgtaaactcc cgtccaacgg gcagaaaact
     6421 gatctaccgg catgccgtcg aacggtgcac cgtatttcca ggcatagtct atggcaggct
     6481 ctatgacagc ctgaaaaagt tctccggtaa gattacgagt attataatat tcgctcttaa
     6541 aaccgttttg cgtaaaactg tcatccgtat aaatctgttg ggacatatcc ttataaagaa
     6601 ggctgtcaga cagcagttct atctgtctgc cgcccttcaa ctgaaccata ccgtcataga
     6661 gcgacactac cgaataagga gtgacaaatc cgcttcctcc tcctgtggga ataatatcgg
     6721 cattaggccc gattaccaat gttctaccgt tccggtaagg caacgtaccg ttgtcatttt
     6781 tcaataacac tactccttcc ctcgccaagt caagtgcaat ttccttagac tgtgcattgt
     6841 ctttatcgat agatttatct tctctcggag tatcaagtgc tccaaaagca atcaatg
//
