LOCUS       gnlProkkaPPNDKLOK_23    5913 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PPNDKLOK_23.
ACCESSION   gnlProkkaPPNDKLOK_23
VERSION     gnlProkkaPPNDKLOK_23
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 00:45:27
            Original ID  :: gnl|Prokka|PPNDKLOK_23
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5913
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5913
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[62:4330]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      63..4330
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..5913
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..5913
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             complement(63..1166)
                     /NRPS_PKS="Domain: Aminotran_5 (30-298). E-value: 6.2e-34.
                     Score: 109.3. Matches aSDomain:
                     nrpspksdomains_ctg23_1_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 80.5; E-value:
                     1.9e-24)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg23_1"
                     /sec_met_domain="Aminotran_5 (E-value: 4.6e-32, bitscore:
                     99.9, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIERKILLNPGPATTTDTVKLAQVVPDICPREKEFAGMMKQLRD
                     DLVRVAHGDLSKHTAVLFCGSGTINIDICLNSLLPADKKVLVVNNGAYSTRAVEVCEYY
                     GLPHIDLKFSVYELPDLSVIEKTLSDNPDIALVYTTHHETGTGILNPIREIGKIAHSHG
                     AVFVVDTTSSLGMIPFDIGKDNVDFCMASAQKGLQAMTGLSFIIGDESLIRASRDYPKR
                     SYYCNLYLQYDYFEKTGEMHFTPPVQTVYAARQALDEYFAVGEEAKFARHKRVFEAIHK
                     GLQELGLQYAIKREWQSGLVVSVLYPDDPLWDFDKVHDYCYERGFTIYPGKISTTNTFR
                     LCALGEIDVEDIERFFEVMREAVKFVK"
     aSDomain        complement(273..1076)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg23_1_Aminotran_5.1"
                     /evalue="6.20E-34"
                     /label="ctg23_1_Aminotran_5.1"
                     /locus_tag="ctg23_1"
                     /protein_end="298"
                     /protein_start="30"
                     /score="109.3"
                     /tool="antismash"
                     /translation="PREKEFAGMMKQLRDDLVRVAHGDLSKHTAVLFCGSGTINIDICL
                     NSLLPADKKVLVVNNGAYSTRAVEVCEYYGLPHIDLKFSVYELPDLSVIEKTLSDNPDI
                     ALVYTTHHETGTGILNPIREIGKIAHSHGAVFVVDTTSSLGMIPFDIGKDNVDFCMASA
                     QKGLQAMTGLSFIIGDESLIRASRDYPKRSYYCNLYLQYDYFEKTGEMHFTPPVQTVYA
                     ARQALDEYFAVGEEAKFARHKRVFEAIHKGLQELGLQYAIKREWQS"
     CDS             complement(1163..2299)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 70.6;
                     E-value: 1.9e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg23_2"
                     /sec_met_domain="TPP_enzyme_N (E-value: 9e-23, bitscore:
                     69.4, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 3e-21, bitscore:
                     64.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVSVEKIYDTFLHHGVDFFTGVPDSLLKNICAYITDHAPRGKHII
                     AANEGSAVGIACGYYMASGNVPLVYMQNSGLGNTVNPLLSLADEKVYSLPLLLLVGWRG
                     EPGTKDEPQHKKQGEVTLDLLKAMKIPFIILDTDEDEALAQIHDAVVSAKSRSIPHAIV
                     IRKDTFGKYKLRQEALNDYPLSREEALMQVVDHLGDNDIVVSTTGKLSRELFEYRESKG
                     QGHARDFLTVGSMGHSSSIALGIALEKPDRRVYCLDGDGAFIMHMGAVSNIGDLSPKNY
                     RHVLFNNGAHESVGGQPTLGFRLDIASIAKGCGYAHVLTASDKEGLSCALEKLSGLSGP
                     VLLEIKVRIDSRDDLGRPTTTPVENKEHFMAFLKDTHS"
     CDS             complement(2311..3612)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 214.1;
                     E-value: 3.9e-65)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg23_3"
                     /sec_met_domain="CTP_transf_like (E-value: 1.5e-13,
                     bitscore: 39.9, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 5.5e-123, bitscore:
                     398.2, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 2.8e-65, bitscore:
                     208.8, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKKIYVGMTADIMHPGLIHIIHEATKYGDVIVGLLTDKAIAEHK
                     RLPYLTYDQRKEVVENIKGVCEVVPQEDWSYVPNLEKLKPDYIIHGDDWKTGPLREVRE
                     QVFEVMNAQGGKVIEIPYTRGINSSSLDKDIKAIGTTPDVRLKSLRRLIQAKPVVRILE
                     AHDGLCGLIIENLEVQKGDKREVFDGMWSSSLTDSTSKGKPDIEAVDLTTRLQDLNNIL
                     ECTTKPIIFDGDTGGKIEHFVFTVRTLERHGISAVIIEDKVGLKKNSLFGTDAIQTQDS
                     IEGFCDKIRAGKNAQVTGDFMIIARIESLIAGKPMSDALERAYAYVQAGADGIMIHSKD
                     KSGEDIREFCRTFRKEYAHVPIVVVPTTYDHVHESELHEWGANVVIYANHLLRAAYPAM
                     MNVARTILENERAEEVRPLCMPIKEILELIPGTK"
     CDS             complement(3632..4330)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg23_4"
                     /sec_met_domain="NTP_transf_3 (E-value: 6.8e-15, bitscore:
                     44.4, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAGLGTRFGHYTELVPKGFVEVGGKPMIIRSIETLLSCGIERII
                     LGTGYKKEAYEALQADFPQIETCFSPRYADTNSMYTLYNTRDVIGDDSFLLLESDLVFE
                     RKAILSLLDDEFPDVMLVSSLTKFQDQYYVEYDRNHILTSCSVDKDALEAKGELVGIHK
                     LSNTFYRRMCADYATILESQPKLGYEYELLRMSRSVSPVRVLRVEGLKWYEIDDEADLS
                     YAEEHIIRYC"
     CDS             complement(4344..5627)
                     /locus_tag="ctg23_5"
                     /transl_table=11
                     /translation="MIRSFLSRSPLYVLALYASVLIFLVYTCAYAYRKPFTAAIYEGEI
                     LWGFDVKILYVLSEIIGYALSKFIGVRILPSMKAGHRIYYIIGLLTFSEVALLGFALLP
                     VPLKVCSIFLSGLPLGMIWGVIFSYIEGRRISEILNVGLSVALIVSSGLVKTLGQFVMD
                     NLHVSEYWMPFTTGLLVYPVMLLCSWLLNQIPAPNEQDIIQRTKRAPMDGKDRRKFLRQ
                     FFWGICMLVVFYGALTVFRELRDSFAADIWKELHIEGAMIFTQTEVPIAVFVLVLMFLI
                     VFVRNNRLALNIIYCIAVTGSSLMVFSTLLYVYGFLSPIWWMILSGLGLYMGYIPFTYL
                     IERLIASLRVVSTAVFIIYLADSFGYLGTTGVFMVKNFVSADISWTVMLMRTALFSGFV
                     SVLSIFAIYCYFKQQLNSLILIPSEIHD"
     CDS             complement(5634..5912)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) dTDP_sugar_isom"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg23_6"
                     /sec_met_domain="dTDP_sugar_isom (E-value: 2.4e-30,
                     bitscore: 94.1, seeds: 19, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFGKYFSVELTEDNHRQFFIPRGFAHGFSVLSEEVIFQYKCDNFY
                     HPEAEGAIVWNDTELKIDWKIPIEKITLSEKDKAYPNFMDQDFHFNY"
ORIGIN
        1 gaagctttct taaatctgaa ccctctttat tatatctatt taagtcttcc attaatacaa
       61 atttatttaa caaatttcac agcttctctc atcacctcaa agaacctctc aatatcctcc
      121 acatcaatct ctcccaacgc acaaagccgg aatgtattcg ttgtggaaat cttacccggg
      181 tagatcgtaa agccacgttc atagcagtaa tcatgaacct tatcaaaatc ccagagggga
      241 tcatcaggat acaatacaga aacaacaaga cccgactgcc attcacgttt gatcgcatac
      301 tgaagaccaa gctcttgcaa acctttatgg atggcttcaa agaccctctt gtgacgggcg
      361 aatttagcct cttcacctac tgcaaaatat tcatcaagag cctgacgggc agcgtaaacg
      421 gtttgaacag gaggagtaaa atgcatctcg cccgtcttct caaagtaatc gtattgtaaa
      481 tagaggttac aatagtagga acgtttcgga taatcccttg aagcacgtat cagggattca
      541 tcacctatga taaaggaaag ccccgtcatg gcctgaaggc ctttctgagc cgaagccata
      601 caaaagtcaa cattatcctt cccgatatca aaaggaatca tacccaggga ggaagtggtg
      661 tcgacaacaa aaacggcacc gtgcgaatgg gctatttttc cgatctcacg gatggggttc
      721 aagatacccg tacccgtttc atggtgcgtg gtatagacca aagcgatgtc cggattgtcg
      781 gaaagcgtct tttcgattac cgacaggtcg ggaagttcgt aaacggaaaa cttcaaatcg
      841 atatggggaa gaccgtaata ttcgcagact tccaccgcac gggtgctgta ggcaccgtta
      901 ttgacaacca gaactttctt atccgcagga agaagagagt tcaggcagat atcgatattg
      961 atcgtacccg agccgcaaaa caggacggcc gtatgtttgg agaggtctcc atgcgcaacg
     1021 cggaccaggt cgtcacgtaa ctgtttcatc atacccgcaa actctttctc acgcggacag
     1081 atatcgggaa caacctgcgc cagcttcacc gtatccgtcg tggtggcggg acccggattc
     1141 aaaaggatct ttctttcgat attcatgaat gcgtatcttt taaaaatgcc ataaaatgct
     1201 ctttattctc taccggagta gtcgtagggc gacccagatc atcacgtgaa tcgatccgga
     1261 ccttaatttc aagaagaacc gggccggaca ggccggacag cttctcaagc gcacacgaaa
     1321 gcccttcctt gtcggaggca gtcagtacat gcgcataacc gcaacctttc gctatcgagg
     1381 cgatatccag acggaaaccg agcgtgggtt gaccgccgac agattcatgg gcaccgttat
     1441 tgaaaaggac gtgcctgtaa ttcttgggag aaagatcgcc gatattgctg acagcaccca
     1501 tgtgcatgat aaaggcaccg tcaccgtcaa gacagtagac acggcggtca ggcttctcaa
     1561 gggcaatccc cagggcaatg gaagaactgt gacccatgga acccacagtt aggaaatccc
     1621 ttgcgtgccc ctgccctttc gattcacggt actcgaaaag ctcacgggag agcttgcccg
     1681 tggtggaaac cacaatgtca ttgtccccga ggtggtcgac aacctgcatt agcgcctcct
     1741 cgcgggacag aggatagtca ttcaaggctt cctgacgcaa tttatatttt ccaaacgtat
     1801 ctttacggat gacaatcgca tggggaatcg accggctctt cgccgagaca accgcgtcat
     1861 ggatctgcgc cagggcctcg tcttcatccg tatccagaat tataaaagga attttcatgg
     1921 ctttcagaag atcaagggta acctcgccct gcttcttatg ctggggttcg tctttcgtgc
     1981 cgggctcacc gcgccaaccg accagcaaaa gaagaggaag gctgtacact ttctcgtccg
     2041 caagggacaa gaggggattc accgtatttc ccagaccgga gttctgcata taaaccaaag
     2101 gaacgttgcc agaagccata taataaccgc aggcaatgcc caccgcggaa ccttcattgg
     2161 cggcgatgat atgcttacca cgcggagcat gatccgtgat ataggcgcaa atattcttca
     2221 aaagggaatc gggaacaccc gtaaagaagt caacaccgtg atgaaggaac gtatcgtata
     2281 tcttttctac acttaccata accgtctgat ttatttagtg cccggaatca attccagaat
     2341 ttccttgata ggcatacaaa gcggacggac ctcctcggcg cgctcgtttt caaggatggt
     2401 cctggccacg ttcatcatgg ccggataagc ggcacggaga aggtggttgg cgtaaatgac
     2461 tacgttggca ccccattcgt gaagttcgga ttcgtgcaca tggtcataag tcgtcggaac
     2521 aacgacaatg ggaacatggg cgtattcttt gcggaaagtc cggcagaact cacggatatc
     2581 ctcacccgac ttatccttgc tgtggatcat gatgccgtcc gcacccgcct gtacataggc
     2641 ataagcacgt tcgagggcgt cactcatggg tttgccggca ataaggcttt cgatacgcgc
     2701 aatgatcata aagtcaccgg tgacctgggc attcttccct gctctgatct tatcgcagaa
     2761 accctcgata ctatcctgag tctggatagc gtcagtacca aaaagggaat tcttcttcag
     2821 accaacttta tcttcaatga ttaccgcaga aatgccgtga cgttccaaag tgcgcacagt
     2881 aaagacaaag tgttcgatct tgccacccgt gtctccatca aaaataatag gcttggtggt
     2941 gcattcaagg atattgttca ggtcctgtaa acgggtggta aggtcgaccg cttcaatatc
     3001 cggtttaccc ttactcgtgg agtcggtcag gctgctggac cacatgccgt caaagacctc
     3061 gcgcttatca cccttctgga cttcaagatt ctcaataatc agaccgcaaa gaccgtcgtg
     3121 agcctccagg atacggacca caggcttggc ctggatcagg cggcggaggg acttaagacg
     3181 tacgtcaggg gtagtcccga tcgccttgat atccttgtcg agcgaggagg agttgatgcc
     3241 ccgggtataa gggatctcga ttaccttgcc cccctgggcg ttcatcacct caaaaacctg
     3301 ttcgcgcacc tcgcgcaacg ggcccgtttt ccagtcgtcg ccgtggatga tatagtcagg
     3361 cttgagtttt tccagattcg gaacatagct ccaatcctcc tggggaacaa cttcgcaaac
     3421 ccctttgata ttctcaacaa cctccttacg ctgatcatag gtaaggtaag ggaggcgttt
     3481 gtgttcggcg atggccttgt ccgtcagcag accgacgatg acatcaccgt atttagtagc
     3541 ttcatgaata atatggatca accccggatg catgatatcc gcggtcatac cgacatagat
     3601 tttcttgttc atatatggaa aagatttgtt tttaacaata gcggatgatg tgctcctcag
     3661 catacgaaag gtcggcttca tcatcgattt cataccattt aagaccctcc accctgagca
     3721 cacggacagg ggaaacggaa cgggacatgc ggagcagttc gtactcgtaa cccagtttag
     3781 gctgggactc caggatggtc gcataatcgg cgcacatccg gcggtagaaa gtattggaaa
     3841 gtttatggat accgaccagc tcgccttttg cctcaagggc gtccttgtct accgaacagg
     3901 aagtaaggat gtgattgcgg tcatattcca cataatactg atcctggaac ttggtcagag
     3961 aagagacaag catcacatcc gggaattcgt catccagcag cgagaggatg gccttgcgtt
     4021 caaaaacaag gtccgattca agcagcagga aactgtcatc gcctatcacg tcacgcgtat
     4081 tgtacagagt gtacatactg ttagtatcgg cataacgggg actgaaacag gtctctatct
     4141 gggggaaatc cgcctgaagg gcttcataag cctctttttt ataacccgta ccgaggatga
     4201 tacgttcgat accgcaggaa agaagggttt cgatagagcg gataatcata ggttttccac
     4261 caacctccac aaaacctttg ggcacaagct cggtataatg cccgaagcgg gtaccaagac
     4321 cggcagccat gatcattgct gttttaatca tgaatttcag agggaattaa tataagtgaa
     4381 ttcaattgtt gcttgaaata acagtaaatc gcaaaaatcg acaggacaga aacaaatcct
     4441 gaaaaaaggg cagtacgcat cagcatgaca gtccaggaaa tgtcagcaga aacgaagttc
     4501 ttgaccataa acacacccgt agtgcccaga tagccgaaag aatcggccaa ataaataata
     4561 aaaacagccg tactaaccac cctaagggaa gcaatcagac gttctatcag gtaagtgaaa
     4621 ggaatatacc ccatatacag accaagaccg gaaagaatca tccaccaaat aggagaaaga
     4681 aaaccgtaca cataaagcaa tgtggaaaaa accattaagg aactgcctgt gacggcgatg
     4741 caataaatga tattaagagc caaacggtta ttacggacaa acacaatcag gaacatcagt
     4801 acaaggacaa aaacggcaat gggaacctct gtctgagtaa agatcattgc accctcaata
     4861 tggagctcct tccaaatatc ggcagcgaaa gagtcacgaa gctcacggaa cacggtcagg
     4921 gcaccgtaaa aaacaaccag catgcagatt ccccaaaaga attgcctcaa aaatttacgc
     4981 ctgtctttac catccatcgg agcacgtttg gtacgctgta tgatatcttg ctcattggga
     5041 gccggaatct gattgagaag ccagctgcaa agcaacataa cagggtatac caacaaaccg
     5101 gtggtaaaag gcatccaata ttccgacaca tgcaaattat ccatcacgaa ttgtcccaag
     5161 gttttcacca aaccggaaga gactatcaaa gcaacgctca gcccgacatt caatatttca
     5221 ctgatacgac gtccttcgat atagctaaaa atgacacccc agatcatacc caggggaaga
     5281 ccgctcagaa aaatagaaca caccttcaaa ggaaccggaa gaagtgcaaa acccaacaaa
     5341 gcaacttcgg aaaaagtaag cagccctatt atataataga tgcggtgacc cgctttcatc
     5401 gaaggcagaa tacgaacacc gatgaacttg gaaagggcgt agccgataat ctcagaaagg
     5461 acatacagga ttttgacatc gaatccccaa agaatctcac cttcataaat ggctgcggta
     5521 aatggcttac ggtaggcata ggcacaagta taaaccagaa aaatcaaaac gctggcatag
     5581 agagccaaaa cgtaaagggg agaacgggaa agaaaggaac gaatcattat cttctagtaa
     5641 ttaaaatgaa agtcttgatc catgaaatta ggatatgctt tatccttttc gctcaatgta
     5701 attttttcaa ttggtatctt ccaatcaatc ttcaattctg tatcattcca aacaatcgcc
     5761 ccttccgctt ccggatgata aaaattatca catttatatt gaaaaatgac ctcttcactt
     5821 aatacactaa agccatgagc gaatcctctt ggaataaaaa actgacgatg attatcttct
     5881 gttagttcta ctgaaaaata tttcccaaat gtc
//
