LOCUS       c00061_gnlPro..         4676 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BBAMPMJL_74.
ACCESSION   c00061_gnlPro..
VERSION     c00061_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-30 03:51:09
            Original ID  :: gnl|Prokka|BBAMPMJL_74
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4676
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4676
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2086:4674]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2087..4674
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4676
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4676
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(34..471)
                     /locus_tag="ctg61_1"
                     /transl_table=11
                     /translation="LPEMAKIAGIDLQSYGLAMLKAGTNLAAKSDMDIIEGDAKSFTMG
                     GKSIRIGQVNTVDLDDIYARQDELVAAMTKEAQAKGYDDFLLIATDILNSNSTGFAVGA
                     DRDKVAAAFNEKFDANDRLALPGVVSRKKQVVPPMQGAFEG"
     CDS             complement(455..967)
                     /locus_tag="ctg61_2"
                     /transl_table=11
                     /translation="MTTLIFGHQNPDTDAITSAMSWAEFQKQAGNTDVEAVALGEPNDE
                     TKFVLDHFKVAAPRVIKTAANETGHVMLVDHNEFQQSVADIEDVQIDSVVDHHRIANFH
                     TASPLYYRAEPVGSTNTVIFELFQEHGYEISPTPGRLNGFWLDFGYPASEVTDCNRQGQ
                     KDLAGNG"
     CDS             complement(997..1965)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     55.5; E-value: 1.4e-17)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg61_3"
                     /sec_met_domain="LysR_substrate (E-value: 8.8e-28,
                     bitscore: 85.5, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 1.1e-18, bitscore: 55.5,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKQERIFSSKTLTYFLQLAETMNYTQSAQLLGITQPALTQQIK
                     KLERTVGAPLFYSVGKKLHLSDAGRTMLKATHEIYEILNQTTDEIQQSTSATVGKINIG
                     LLSSIEDSAFNAFIAHYYDLNPEVEITLRMMTRHEIWEALENNQIDLAIMNLPDETIKN
                     WKPYASKKILDEQLLFLHHDNSLANRKRLHFKDTLAEPWVTYPDDYFLGQLLHEAYKNQ
                     MVDFPHVVAHFTTPYQLTQFVNHTGVNTALPTSFVAAHADQIKAHAVAFEPAIRFETSF
                     VYRKEKADIPRIANFFKTFDAHLSEKDYLSRVSENSGKIER"
     CDS             complement(2087..2938)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 66.6;
                     E-value: 9e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg61_4"
                     /sec_met_domain="Radical_SAM (E-value: 2.4e-27, bitscore:
                     84.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLIDQSQSPLNILEQVGQDHEGPIKGYVHSVESFGSVDGPGIRF
                     VVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILADAQRYKAFWGEQGGITCSGGESLV
                     QIDFILELFTKAKELGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAE
                     HKKLTGFPNENILDMVQYMSDHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKRVVQ
                     KVEILPYHTLGVKKYHELGITYPLEGIEPPSADRVKNAENLLHVEDYTGWQSWRPQPAN
                     KQ"
     CDS             complement(3076..4674)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg61_5"
                     /sec_met_domain="Gly_radical (E-value: 1.2e-42, bitscore:
                     133.4, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2.3e-171, bitscore:
                     559.6, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="NEQYRALNDMKKMAKEYGYDISRPAANAQEAVQWIYFGYLAAVKT
                     QNGAAMSVGRIDTVIDAYIQRDMRLGKLNEEQAQELIDHLVMKLRMVRFIRTEEYNSLF
                     SGDPIWATLSLAGMGNDGRHHVTKTAFRFLKTLDNMGAAPEPNITLLWSERLPEGFKRY
                     ATEVSIQSSTIQYENDDLMRNEWGTDYYGIACCVSAQPIADGVQYFGARANLAKTVLYA
                     INGGKDEIQEAQVGPEYEPITSDYIDYDEFMKKFDKMMDWLADVYVNALNVIHYMHDKY
                     YYEAAQLALKDTRLNRTFATGISGLSHAVDSISAIKYGHVKAIRDENGVAIDFVADNDD
                     YPRYGNNDDRADNIAKWLVKTFYNKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTTPN
                     GRQKGEPFSPGANPAYGAEKNGALASLMSTAKIPYHYATDGISNTFGVTPNTLGHDDET
                     RKDTLVHMVDGYMENSGMHLNINVFNKETLIDAQKHPEEYPTLTVRVSGYCVYFADLTK
                     EQQDDVIARTFFDEM"
ORIGIN
        1 tgcgatttat aaaaccaatt attaagttca aaattaacct tcaaacgcac cttgcattgg
       61 cggtacgacc tgcttcttac gtgaaaccac gccaggcagt gccaaccggt catttgcatc
      121 aaacttttcg ttgaatgctg cggccacttt gtcacgatct gcgccaacag caaagcccgt
      181 tgaattgctg ttcagaatat cggttgcaat taacaggaag tcatcatagc ccttcgcctg
      241 tgcttctttg gtcatagcgg ccaccaattc atcctgacgc gcataaatat catcaagatc
      301 aactgtattc acttgtccaa tacggataga cttaccgccc atcgtaaagc tcttcgcatc
      361 gccttcaatg atatccatat ccgacttggc agctagattg gtgcccgcct tcaacatagc
      421 caagccatat gattgcaggt caatgccagc gattttagcc atttccggca agatcttttt
      481 gtccttgtcg gttgcagtcg gtgacttcag aagcagggta tccgaaatca agccagaagc
      541 cattaagcct gccaggtgtt ggtgaaattt catagccgtg ttcctggaac aactcgaaaa
      601 tcaccgtgtt ggtactgccc actggttctg cccggtaata tagtggtgac gccgtatgga
      661 agttggcaat gcggtggtga tcaaccactg aatcgatttg cacgtcttcg atatcagcaa
      721 cgctttgctg gaattcgtta tgatcaacca acattacgtg cccggtttca tttgcggctg
      781 tcttaatgac ccgcggcgca gcaaccttaa aatgatccaa tacaaacttg gtttcatcgt
      841 ttggttcacc gagtgctact gcttcaacat cggtattacc tgcttgcttc tgaaattcag
      901 cccaactcat ggcgcttgta atcgcgtctg tatcagggtt ttgatgtcca aaaatcaaag
      961 tcgtcatgtt atctgatcgc tcctcagctg aatagtttac cgttcaattt taccactgtt
     1021 ttcgctgacc cgcgatagat aatctttctc ggataaatgt gcgtcaaacg tcttaaagaa
     1081 gttggcaatc cgcggaatat cggctttttc cttgcgatac acaaacgagg tctcaaagcg
     1141 aatcgctggt tcaaaggcaa cggcatgcgc cttgatttga tcagcatgag cggcaacaaa
     1201 actggttggt aacgcggtgt taacaccggt atggttgaca aactgagtta actgatatgg
     1261 cgtggtaaaa tgcgccacaa catgcggaaa atcaaccatc tgattcttat acgcctcatg
     1321 caacaactga cccaggaaat agtcgtctgg ataagttacc cacggctcag ccagcgtatc
     1381 tttaaaatgc aggcgtttgc gattagccaa ggaattatca tgatgcagga acaataactg
     1441 ttcatcaaga atcttcttgc ttgcatacgg cttccagttc ttaatcgtct catcaggcag
     1501 attcatgatg gccaaatcaa tctgattgtt ctccaacgct tcccagatct catgacgtgt
     1561 catcattcgt agcgtgattt cgacttcagg attaaggtcg taataatgcg cgataaaagc
     1621 attaaacgca ctgtcttcaa ttgaacttaa caagccaata ttaatcttgc cgactgtggc
     1681 actggtgctt tgctgaatct cgtcagtcgt ctggttcaaa atttcataaa tctcgtgtgt
     1741 cgccttcaac atcgtgcgac cggcatcgga taaatgcaac ttcttaccaa cggaataaaa
     1801 caatggtgcc cccactgttc gttccaattt cttgatttgc tgcgtcagtg ccggctgggt
     1861 aatccccaac aactgggctg actgggtgta gttcatggtt tctgctaatt gcaaaaaata
     1921 cgtgagagtt ttggaggaaa aaatgcgctc ttgtttcgtc ttcatagggc ggcttccctt
     1981 tcgtttataa tttgataacg ttactcaacc atcatcataa gtggcattta aaaaaaacgc
     2041 aacggatttg ctgcgttttt aatcgttacg ttaagtttca actaaatcac tgcttgttag
     2101 ctggctgtgg tcgccaactt tgccatccag tatagtcctc aacgtgtagc aagttctctg
     2161 cattcttgac ccgatcagct gaaggcggct cgataccttc cagcggatac gtaataccta
     2221 gttcatggta cttcttaacc cctaacgtat ggtacggaag aatttccact ttctggacca
     2281 cgcgtttatc caaagtggca atataatctc ccaagcgttt gagataagga tcaaagtcgg
     2341 ttcgttctgg cacaagtacg tggcgaatcc acatatcatc gccatgatcc gacatatact
     2401 gaaccatatc caaaatgttt tcgtttggga aaccggttag ctttttgtgt tccgcggaat
     2461 caatgtgctt gatgtcaacc aacgaaatat ccgtgacggc catcagtttc tcaaattgac
     2521 caaaccacgg ctgatcacgg gtgaatggac cacccgaagt atccaaacaa gttgagatac
     2581 ccagttcctt agctttggta aaaagttcca ggataaaatc aatttggacg agactttcgc
     2641 caccgctaca agtaatcccc ccttgttcgc cccagaaggc tttatatcgc tgtgcatctg
     2701 ccaggatctg atcagcggtc atttcctcgc ccacaccgat attccaagtg tcagggttgt
     2761 gacagtactg gcaccgcatc ctgcatcctt gcataaagac gacaaaccga atcccgggac
     2821 catcaaccga accaaagctt tcgactgaat gaacgtatcc ttttattggg ccttcatgat
     2881 cctgtcccac ttgttccaaa atatttaacg ggctttgtga ttgatcgatg agtttcatct
     2941 atgccgcctc cagtgcaaat aaatacctaa ttttcaacga ttttcatttc agtttgtcaa
     3001 aaaacggcaa cagcatcgtt gccgtctttt gacaaaacag gaatgatctc ctgttttgcg
     3061 aatagattga cttgattaca tttcgtcgaa gaaggtccga gcgataacat cgtcttgctg
     3121 ttctttggtc aggtctgcaa agtagacgca gtaaccggaa actcgaacag tcaaggttgg
     3181 atattcttct gggtgcttct gggcatcaat caacgtttct ttattgaaga cgttgatgtt
     3241 aaggtgcatg ccgctatttt ccatgtagcc gtcaaccatg tgaaccaagg tgtccttacg
     3301 ggtttcatcg tcatggccta aggtgttcgg tgttacccca aaggtattgc tgatcccgtc
     3361 tgttgcatag tgatatggga tcttggcggt tgacatcaag gaagccaaag caccgttctt
     3421 ttcagcacca tacgccgggt tggcaccagg tgagaatggt tcgcccttct gacggccgtt
     3481 tggtgttgtc ccggtattct taccataaac cacattagat gtaatggtca gaacactggt
     3541 actgagctta gcaccgcgat acagatgatg tgtattcatc ttgttgtaga aggtcttaac
     3601 caaccactta gcaatgttgt ctgcccggtc atcattgtta ccataacgcg gatagtcatc
     3661 attgtcagca acaaagtcga ttgcaacgcc attttcatcc cgaattgcct tcacgtggcc
     3721 atacttgatg gcactgatgg aatcaaccgc atgtgacaaa ccggaaatcc cggtagcaaa
     3781 cgtccgattc agacgtgtat ccttcaatgc caactgcgca gcttcatagt agtacttgtc
     3841 atgcatgtag tgaataacat tcagtgcatt gacatagaca tctgctaacc agtccatcat
     3901 cttgtcgaac ttcttcatga actcgtcata gtcgatatag tcgctggtga tcggctcgta
     3961 ttcaggtccc acctgtgctt cctgaatttc gtctttacca ccgttgatcg catacagcac
     4021 ggtcttggct aggtttgctc gcgccccgaa gtactgaaca ccatctgcaa ttggctgcgc
     4081 ggaaacacag caagcaatgc cgtaatagtc ggttccccat tcgttacgca tcagatcatc
     4141 gttttcgtat tgaatggttg aactttggat ggacacttca gtggcataac gtttgaaccc
     4201 ttcaggaaga cgttcgctcc acaacaaggt gatgtttggt tccggtgccg cgcccatgtt
     4261 gtccaaggtc ttcaggaagc ggaacgcggt cttggtaaca tgatgacggc catcattacc
     4321 catccctgcc aaggacagtg ttgcccagat cggatcgccg gagaacagtg agttgtattc
     4381 ttcagtccga atgaaacgta ccatccgtaa cttcataacc aaatgatcga tgagttcttg
     4441 tgcctgctct tcattcagct tgcccaaacg catatcccgt tgaatatagg catcaatcac
     4501 tgtatcgata cggccgacag acattgccgc accattttgg gtcttaacgg ctgccaggta
     4561 accgaagtag atccactgaa cagcttcttg agcattagca gctgggcggg aaatgtcata
     4621 gccgtattct ttggccatct tcttcatgtc attcaaggca cgatactgtt cgttaa
//
