LOCUS       c00305_gnlPro..         7209 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OOAFFMNO_344.
ACCESSION   c00305_gnlPro..
VERSION     c00305_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-29 04:04:31
            Original ID  :: gnl|Prokka|OOAFFMNO_344
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7209
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7209
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2163:5414]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2164..5414
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7209
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7209
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(112..1044)
                     /locus_tag="ctg305_1"
                     /transl_table=11
                     /translation="MTTLIFGHQNPDTDAITSAMSWAEFQKQAGNTDVEAVALGEPNDE
                     TKFVLDHFKVAAPRVIKTAANETGHVMLVDHNEFQQSVADIEDVQIDSVVDHHRIANFH
                     TASPLYYRAEPVGSTNTVIFELFQEHGYEISPTLAGLMASGLISDTLLLKSPTATDKDK
                     KILPEMAKIAGIDLQSYGLAMLKAGTNLAAKSDMDIIEGDAKSFTMGGKSIRIGQVNTV
                     DLDDIYARQDELVAAMTKEAQAKGYDDFLLIATDILNSNSTGFAVGADRDKVAAAFNEK
                     FDANDRLALPGVVSRKKQVVPPMQGAFEG"
     CDS             complement(1074..2042)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     55.5; E-value: 1.4e-17)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg305_2"
                     /sec_met_domain="LysR_substrate (E-value: 1e-27, bitscore:
                     85.3, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 1.1e-18, bitscore: 55.5,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKQERIFSSKTLTYFLQLAETMNYTQSAQLLGITQPALTQQIK
                     KLERTVGAPLFYSVGKKLHLSDAGRTMLKATHEIYEILNQTTDEIQQSTSAIVGKINIG
                     LLSSIEDSAFNAFIAHYYDLNPEVEITLRMMTRHEIWEALENNQIDLAIMNLPDETIKN
                     WKPYASKKILDEQLLFLHHDNSLANRKRLHFKDTLAEPWVTYPDDYFLGQLLHEAYKNQ
                     MVDFPHVVAHFTTPYQLTQFVNHTGVNTALPTSFVAAHADQIKAHAVAFEPTIRFETSF
                     VYRKEKADIPRIANFFKTFDAHLSEKDYLSRVSENSGKIER"
     CDS             complement(2164..3015)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 66.6;
                     E-value: 9e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg305_3"
                     /sec_met_domain="Radical_SAM (E-value: 2.4e-27, bitscore:
                     84.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLIDQSQSPLNILEQVGQDHEGPIKGYVHSVESFGSVDGPGIRF
                     VVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILADAQRYKAFWGEQGGITCSGGESLV
                     QIDFILELFTKAKELGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAE
                     HKKLTGFPNENILDMVQYMSDHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKRVVQ
                     KVEILPYHTLGVKKYHELGITYPLEGIEPPSADRVKNAENLLHVEDYTGWQSWRPQPAN
                     KQ"
     CDS             complement(3153..5414)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg305_4"
                     /sec_met_domain="Gly_radical (E-value: 2e-42, bitscore:
                     132.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 7.3e-246, bitscore:
                     805.6, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQLDETKVPNYWEGFNGGDWQEEINVRDFIEHNLNQYDGDESFL
                     AGPTEATTVLNNQVLNLKKQERANGGVLDADNNIPSTITSHGPGYLNKDLEKIVGVQTD
                     KPFKRAFMPFGGIRMAEDALKSYGFETDANEHKIFTEYRKTHNQGVFDAYTPEMRKARH
                     YKIVTGLPDAYGRGRIVSDFPRIAIYGIDRLMAEKFNDYNLTGDGEMTDDVIKLREEIN
                     EQYRALNDMKKMAKEYGYDISRPAANAQEAVQWIYFGYLAAVKTQNGAAMSVGRIDTVI
                     DAYIQRDMRLGKLNEEQAQELIDHLVMKLRMVRFIRTEEYNSLFSGDPIWATLSLAGMG
                     NDGRHHVTKTAFRFLKTLDNMGAAPEPNITLLWSERLPEGFKRYATEVSIQSSTIQYEN
                     DDLMRNEWGTDYYGIACCVSAQPIADGVQYFGARANLAKTVLYAINGGKDEIQEAQVGP
                     EYEPITSDYIDYDEFMKKFDKMMDWLADVYVNALNVIHYMHDKYYYEAAQLALKDTRLN
                     RTFATGISGLSHAVDSISAIKYGHVKAIRDENGIAIDFVADNDDYPRYGNNDDRADNIA
                     KWLVKTFYNKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPA
                     YGAEKNGALASLMSTAKIPYHYATDGISNTFGVTPNTLGHDDETRKDTLVHMVDGYMEN
                     SGMHLNINVFNKETLIDAQKHPEEYPTLTVRVSGYCVYFADLTKEQQDDVIARTFFDEM
                     "
     CDS             complement(5659..7113)
                     /locus_tag="ctg305_5"
                     /transl_table=11
                     /translation="MVAIADMQPKLLGLKAMLEAYVAHRRDVVTRRTRYELNKAQARQH
                     IVEGLIKMLSILDQVIAAIRASSDKGDAKRNLVKQFDFSEAQAEAIVSLQLYRLTNTDI
                     TALQDEAAQLAKAIAEYQDILAQPASRDRVIEKELKRIAKEYGDDRRSSIQAEIETLEV
                     ATTVTVADETVMVQVSRDGYVKRSSLRSYQAVDPEDNGLKPNDLAIFTGELSTLQHLYI
                     VTNAGNIIYRPVYEISDARWKDTGEHLSQTVGLGTDEKVLAVFAFDQLDLAGTFVLGSS
                     DGYIKQTALSDLQPQRTYKRKPMMAMKLKTPGAVVTNAYFTTDQAQDVFVVSRHAYGLD
                     FPLAEVSTVGARATGVKSMDLKPEDEVVNFILVKQPEKAVVGILTQRGAFKRMALSEVG
                     QMSRARRGLLVLRELKRDPHRIVAMMQVDDNTRLDVLTDADKVITLTPVNHPTGDRYSN
                     GSFVIDTDVQGKPVFVRTREETPVEP"
ORIGIN
        1 caccttcagt tctaaaaatt cganatttta tgttcatcca agtttttgag cncaaaaaaa
       61 tacgcaacca caaacggctg cgatttataa aaccaattat taagttcaaa attaaccttc
      121 aaacgcacct tgcattggcg gtacgacctg cttcttacgt gaaaccacgc caggcagtgc
      181 caaccggtca tttgcatcaa acttttcgtt gaatgctgcg gccactttgt cacgatctgc
      241 gccaacagca aagcccgttg aattgctgtt cagaatatcg gttgcaatta acaggaagtc
      301 atcatagccc ttcgcctgtg cttctttggt catagcggcc accaattcat cctgacgcgc
      361 ataaatatca tcaagatcaa ctgtgttcac ttgtccaata cggatagact taccacccat
      421 tgtaaagctc ttcgcatcgc cttcaatgat gtccatatcc gacttggcag ctagattggt
      481 gccggccttc aacatagcca agccgtatga ttgcaggtca atgccagcga ttttagccat
      541 ttccggcagg atctttttgt ccttgtcggt tgcagtcggt gacttcagaa gcagagtatc
      601 cgaaatcaag ccagaagcca ttaagcctgc cagtgttggt gaaatttcat agccgtgttc
      661 ctgaaacaac tcgaaaatca ccgtgttggt actgcccact ggttctgccc ggtaatatag
      721 cggtgacgcc gtatggaagt tggcaatgcg gtggtgatca acaactgaat cgatttgcac
      781 gtcttcgata tcagcaacgc tttgctggaa ttcgttatga tcaaccaaca ttacgtgccc
      841 ggtttcattt gcggctgtct taataacccg cggcgcagca accttaaaat gatccaatac
      901 aaacttggtt tcatcgtttg gttcaccgag tgctactgct tcaacatcgg tattacctgc
      961 ctgcttctga aattcagccc aactcatggc gctggtaatc gcgtctgtat cagggttttg
     1021 atgtccaaaa atcaaagtcg tcatgttatc tgatcgctcc tcagctgaat agtttaccgt
     1081 tcaattttac cactgttttc gctgacccgc gatagataat ctttctcgga taaatgtgcg
     1141 tcaaacgtct taaagaagtt ggcaatccgc ggaatatcag ctttttcctt gcgatacaca
     1201 aacgaggtct caaagcgaat cgttggttca aaggcaacgg catgcgcctt gatttgatca
     1261 gcatgagcgg caacaaaact ggttggtaac gcggtgttaa cgccggtatg attgacaaac
     1321 tgagttaact gatatggcgt ggtaaaatgc gccacaacat gcggaaaatc aaccatctga
     1381 ttcttatatg cctcatgcaa caactgaccc aggaaatagt cgtctggata agttacccac
     1441 ggctcagcca gcgtatcttt gaaatgcagg cgtttgcgat tagccaagga attatcatga
     1501 tgcaggaaca ataactgttc atcaagaatc ttcttgctgg catacggctt ccagttctta
     1561 atcgtctcat caggcagatt catgatggcc aaatcaatct gattgttctc caatgcttcc
     1621 cagatctcat gacgtgtcat cattcgtagc gtgatttcga cttcgggatt aaggtcgtaa
     1681 taatgcgcga taaaagcatt aaacgcactg tcttcaattg aacttaacaa gccaatatta
     1741 atcttgccga ctatggcact ggtactttgc tgaatctcgt cggtcgtctg gttcaaaatt
     1801 tcataaatct cgtgtgtcgc ctttaacatc gtgcgaccgg catcggataa atgcaacttc
     1861 ttaccaacgg aataaaacaa tggtgccccc actgttcgtt ccaatttctt gatttgctgc
     1921 gtcagtgccg gctgggtaat tcccaacaac tgggctgact gggtgtagtt catggtttct
     1981 gctaattgca aaaaatacgt gagagttttg gaggaaaaaa tgcgctcttg tttcgtcttc
     2041 atagggcggc ttccctttcg tttataattt gataacgtta ctcaaccatc atcataagtg
     2101 gcatttaaaa aaaacgcaac ggatttgctg cgtttttaat cgttacgtta agtttcaact
     2161 aaatcactgc ttgttagctg gctgtggtcg ccaactttgc catccagtat aatcctcaac
     2221 gtgtagcaag ttctctgcat tcttgacccg atcagctgaa ggcggctcga taccttccag
     2281 cggatacgta atacctagtt catggtactt cttaacccct aacgtatgat acggaagaat
     2341 ttccactttc tgaaccacgc gtttatccaa agtggcaata taatctccca agcgtttgag
     2401 ataaggatca aagtcggttc gttctggcac aagtacgtgg cgaatccaca tatcatcgcc
     2461 atgatccgac atatactgaa ccatatccaa aatgttttcg tttgggaaac cggtcagctt
     2521 tttgtgttcc gcggaatcaa tgtgcttgat atcaaccaac gaaatatccg tgacggccat
     2581 cagtttctca aattgaccaa accacggctg atcgcgggtg aatggaccac ccgaagtatc
     2641 caaacaagtt gagataccca gttccttagc tttggtaaaa agttccagga taaaatcaat
     2701 ttggacgaga ctttcgccac cgctacaagt aatcccccct tgttcacccc agaaggcttt
     2761 atatcgctgt gcatctgcca ggatctgatc agcagtcatt tcctcgccca caccgatatt
     2821 ccaggtgtca gggttgtgac agtactggca ccgcatcctg catccttgca taaagacgac
     2881 aaaccgaatc ccgggaccat caaccgaacc aaagctttcg actgaatgaa cgtatccctt
     2941 tattgggcct tcatgatcct gtcccacttg ttccaaaata tttaacgggc tttgtgattg
     3001 atcgatgagt ttcatctatg ccgcctccag tgcaaataaa tacctaattt tcaacgattt
     3061 tcatttcagt ttgtcaaaaa acggcaacag catcgttgcc gtcttttgac aaaacaggaa
     3121 tgatctcctg ttttgcgaat agattgactt gattacattt cgtcgaagaa ggtccgagcg
     3181 ataacatcgt cttgctgttc tttggtcagg tctgcaaagt agacgcagta accggaaact
     3241 cgaacagtca aggttggata ttcttctggg tgcttctggg catcaatcaa cgtttcttta
     3301 ttgaagacgt tgatgttaag gtgcatgccg ctattttcca tgtagccgtc aaccatgtga
     3361 accaaggtgt ccttacgggt ttcatcgtca tggcctaagg tgttcggtgt taccccaaag
     3421 gtattgctga tcccgtctgt tgcatagtga tatgggatct tggcggttga catcaaggaa
     3481 gctaaagcac cgttcttttc agcgccatac gctgggttgg caccaggtga gaatggttcg
     3541 cccttctgac ggccgtttgg tgttgtcccg gtattcttac cataaaccac atttgatgta
     3601 atggtcagaa cactggtact gagcttagca ccgcgataca gatgatgtgt attcatcttg
     3661 ttgtagaagg tcttaaccaa ccacttagca atgttgtctg cccggtcatc attgttacca
     3721 taacgcggat agtcatcatt gtcagcaaca aagtcgattg caatgccatt ttcatcccga
     3781 attgccttca catggccata cttgatggca ctgatggaat caaccgcatg tgacaaaccg
     3841 gaaatcccgg tagcaaatgt ccgattcaga cgcgtatcct tcaatgccaa ctgtgcagct
     3901 tcatagtagt acttgtcatg catgtagtga ataacattca gggcattgac atagacatct
     3961 gctaaccagt ccatcatctt gtcgaacttc ttcatgaact cgtcatagtc aatgtagtcg
     4021 ctggtaatcg gctcgtattc aggtcccacc tgtgcttcct gaatttcgtc tttaccaccg
     4081 ttgatcgcat acagcacggt cttggctagg tttgctcgcg ccccgaagta ctgaacgcca
     4141 tctgcaattg gctgtgcgga aacacagcaa gcaatgccgt aatagtcggt tccccattcg
     4201 ttgcgcatca gatcatcgtt ttcgtactga atggttgaac tttggatgga cacttcagta
     4261 gcataacgtt tgaacccttc aggaagacgt tcgctccaca acaaggtgat gtttggttcc
     4321 ggtgccgcgc ccatgttgtc caaggtcttc aggaagcgga acgcggtctt ggtaacatga
     4381 tgacggccat cattacccat ccctgccaag gacagtgttg cccagatcgg atcgccggag
     4441 aacagtgagt tgtattcttc agtccgaatg aaacgaacca tccgcaactt cataaccaaa
     4501 tgatcgatga gttcttgtgc ctgctcttca ttcagcttgc ccaaacgcat atcccgttga
     4561 atataggcat caatcactgt atcgatacgg ccgacagaca ttgctgcacc attttgggtc
     4621 ttaacggctg ccaggtaacc gaagtagatc cactgaacag cttcttgagc attagcagct
     4681 gggcgggaaa tgtcatagcc gtattctttg gccatcttct tcatgtcatt caaggcacga
     4741 tactgttcgt taatttcttc gcgcagtttg ataacatcat ctgtcatctc gccatcgccg
     4801 gtcagattgt aatcgttgaa cttctcagcc atcagccggt caatcccata aatagcaatt
     4861 cgagggaagt ccgaaacgat ccgaccacga ccataagcat ccggtaagcc ggtcacaatc
     4921 ttatagtggc gggctttgcg catttccggc gtataagcat caaaaacacc ttggttatgc
     4981 gtcttacgat attcggtaaa gatcttgtgc tcattagcgt cggtttcgaa gccataggat
     5041 ttcaaagcat cctcagccat ccggataccg ccaaatggca tgaatgcccg cttaaacggc
     5101 ttatcggttt ggacaccgac aatcttttcg aggtccttat tcagataacc tgggccatga
     5161 gaagtaatgg tcgatggaat attgttatct gcatccagca cgccgccatt ggcacgttct
     5221 tgctttttca gattcaaaac ctgattattt aaaacggtcg ttgcttcagt ggggccagca
     5281 aggaaactct cgtccccatc atattggttc aggttatgct cgataaaatc gcgcacgtta
     5341 atttcttctt gccagtcgcc gccgttaaag ccttcccaat aattaggaac tttggtttcg
     5401 tcaagctgtt tcataggtat tgcctcctaa agtgaattac agatgtgaca gttcttacaa
     5461 actcagtata acacgaaggc aaatctatgc aagcgtttta tcgctgatat aacaggcgtt
     5521 ttgctgtttt ttcggtgtac tttcctgttt cataaaaaaa gcttaatcat tagcttgata
     5581 agttgtgcac aaccgtactg tcatataata aatcaaaaag tcctcatctg tattaataca
     5641 gatgaggact tttttgcgtc acggttcaac cggtgtttct tcacgtgtgc gtacgaaaac
     5701 cggtttccct tggacatcag tatcaatcac aaaggaacca ttagagtagc gatcacctgt
     5761 cgggtggttc acaggcgtta acgtgatcac tttatcggca tcggttaaca cgtccagacg
     5821 cgtgttgtca tcgacttgca tcatggcgac aatgcggtgc ggatcacgct ttaattcgcg
     5881 taaaacaagc aatccgcggc gagcacggct catttgaccg acttcgctta acgccatacg
     5941 tttaaacgca ccacgctgag tcaaaatgcc caccactgct ttttctggct gcttaactaa
     6001 aataaagtta actacctcat cttccggttt gagatccata cttttgaccc cagtagcccg
     6061 ggcaccgacc gtggaaactt cagccagtgg gaagtcgagt ccatacgcat gtcggctgac
     6121 aacaaaaaca tcttgggctt gatcggtcgt gaaataagcg ttggtaacca cggcacccgg
     6181 tgttttcagt ttcattgcca tcattggttt gcgtttatac gtgcgctgcg gctgcaaatc
     6241 actcaatgcc gtctgcttaa tgtagccgtc actggagcca aggacaaagg taccagcaag
     6301 atctagctga tcaaacgcaa agactgccaa aaccttttca tcggttccca agccaactgt
     6361 ctggcttaaa tgttccccag tgtccttcca ccgcgcatcg ctaatttcgt aaacgggccg
     6421 ataaatgata ttaccggcat tcgtgacgat gtaaagatgt tgcagggtgc ttaactctcc
     6481 ggtaaaaatc gccaaatcat tgggtttcaa accgttatct tcaggatcaa cagcttggta
     6541 actacgcaag gagctgcgtt tgacatagcc gtcgcgtgat acctggacca ttacggtttc
     6601 atcagccact gtgaccgtcg ttgccacttc gagcgtttca atctcggctt gaatgctgct
     6661 gcgccgatca tccccgtact ctttagcaat ccgtttaagt tctttttcaa tcacccgatc
     6721 acgcgaagct ggctgcgcta gaatgtcttg atattcggca atcgccttgg ccagttgtgc
     6781 cgcctcgtcc tgtaaagcgg taatgtcggt attcgtcaac cgatataact gtaaggagac
     6841 aatagcttca gcctgcgctt cggaaaaatc aaattgtttg accaaattgc gtttggcatc
     6901 ccctttatct gaggatgcac gaatcgcagc aatgacttga tccaaaatgg acaacatttt
     6961 aatcaagcct tcaacgatgt gctgccgtgc ctgtgctttg tttaactcat aacgtgtccg
     7021 gcgtgtgacc acatcacggc gatgcgccac ataagcttcc aacatcgcct tgagcccaag
     7081 caatttcggt tgcatatcgg caatcgcaac catattaaag ttatatgtga tttgcagatc
     7141 ggtattttga agaggtaatt caaaatccca tgggcatccg cttctttttt cagctcaatg
     7201 acaacactt
//
