LOCUS       c00497_gnlPro..         4977 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EMONJBNA_635.
ACCESSION   c00497_gnlPro..
VERSION     c00497_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-30 03:41:24
            Original ID  :: gnl|Prokka|EMONJBNA_635
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4977
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4977
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2410:4977]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2411..4977
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4977
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4977
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(359..1291)
                     /locus_tag="ctg497_1"
                     /transl_table=11
                     /translation="MTTLIFGHQNPDTDAITSAMSWAEFQKQAGNTDVEAVALGEPNDE
                     TKFVLDHFKVAAPRVIKTAANETGHVMLVDHNEFQQSVADIEDVQIDSVVDHHRIANFH
                     TASPLYYRAEPVGSTNTVIFELFQEHGYEISPTLAGLMASGLISDTLLLKSPTATDKDK
                     KILPEMAKIAGIDLQSYGLAMLKAGTNLAAKSDMDIIEGDAKSFTMGGKSIRIGQVNTV
                     DLDDIYARQDELVAAMTKEAQAKGYDDFLLIATDILNSNSTGFAVGADRDKVAAAFNEK
                     FDANDRLTLPGVVSRKKQVVPPMQGAFEG"
     CDS             complement(1321..2289)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     55.5; E-value: 1.4e-17)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg497_2"
                     /sec_met_domain="LysR_substrate (E-value: 7.1e-28,
                     bitscore: 85.5, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 8.8e-19, bitscore: 55.5,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKQERIFSSKTLTYFLQLAETMNYTQSAQLLGITQPALTQQIK
                     KLERTVGAPLFYSVGKKLHLSDAGRTMLKATHEIYEILNQTTDEIQQSTSATVGKINIG
                     LLSSIEDSAFNAFIAHYYDLNPEVEITLRMMTRHEIWEALENNQIDLAIMNLPDETIKN
                     WKPYASKKILDEQLLFLHHDNSLANRKRLHFKDTLAEPWVTYPDDYFLGQLLHEAYKNQ
                     MVDFPHVVAHFTTPYQLTQFVNHTGVNTALPTSFVAAHADQIKAHAVAFEPAIRFETSF
                     VYRKEKADIPRIANFFKTFDAHLSEKDYLSRVSENSGKIER"
     CDS             complement(2411..3262)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 66.6;
                     E-value: 9e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg497_3"
                     /sec_met_domain="Radical_SAM (E-value: 1.9e-27, bitscore:
                     84.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLIDQSQSPLNILEQVGQDHEGPIKGYVHSVESFGSVDGPGIRF
                     VVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILADAQRYKAFWGEQGGITCSGGESLV
                     QIDFILELFTKAKELGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAE
                     HKKLTGFPNENILDMVQYMSDHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKRVVQ
                     KVEILPYHTLGVKKYHELGITYPLEGIEPPSADRVKNAENLLHVEDYTGWQSWRPQPAN
                     KQ"
     CDS             complement(3400..4977)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg497_4"
                     /sec_met_domain="Gly_radical (E-value: 9e-43, bitscore:
                     133.4, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.6e-168, bitscore:
                     549.9, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="NDMKKMAKEYGYDISRPAANAQEAVQWIYFGYLAAVKTQNGAAMS
                     VGRIDTVIDAYIQRDMRLGKLNEEQAQELIDHLVMKLRMVRFIRTEEYNSLFSGDPIWA
                     TLSLAGMGNDGRHHVTKTAFRFLKTLDNMGAAPEPNITLLWSERLPEGFKRYATEVSIQ
                     SSTIQYENDDLMRNEWGTDYYGIACCVSAQPIADGVQYFGARANLAKTVLYAINGGKDE
                     IQEAQVGPEYEPITSDYIDYDEFMKKFDKMMDWLADVYVNALNVIHYMHDKYYYEAAQL
                     ALKDTRLNRTFATGISGLSHAVDSISAIKYGHVKAIRDENGVAIDFVADNDDYPRYGNN
                     DDRADNIAKWLVKTFYNKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEP
                     FSPGANPAYGAEKNGALASLMSTAKIPYHYATDGISNTFGVTPNTLGHDDETRKDTLVH
                     MVDGYMENSGMHLNINVFNKETLIDAQKHPEEYPTLTVRVSGYCVYFADLTKEQQDDVI
                     ARTFFDEM"
ORIGIN
        1 catccgtaca attgatttgc ttctctcaaa agtttttgag ctgtctgtca ggtgagggga
       61 cttgcatcaa tcgttttgcc atttgaacac caaataagtc acgtcaaagt caacgactca
      121 aaatctccat tctagtttac ataatatata ttatacgaag tagttataag gctgcttttt
      181 ttcacctcgt tttaaatttg aaccatcctt actgaactga aagatttcgc caatcatcac
      241 atttttgcac cttcagatct gaaaattcga tattttatgt ttatcaaagt ttttgagcaa
      301 caaaaaaatc gcaaccacaa acggctgcga tttataaaac caattattaa gttcaaaatt
      361 aaccttcaaa cgcaccttgc attggcggta cgacctgctt cttacgtgaa accacgccag
      421 gcagtgtcaa ccggtcattc gcatcaaact tttcgttgaa tgctgcggcc actttgtcac
      481 gatctgcgcc aacagcaaag cccgttgaat tgctgttcag aatatcggtt gcaattaaca
      541 ggaagtcatc atagcccttc gcctgtgctt ctttggtcat agcggccacc aattcatcct
      601 gacgcgcata aatatcatca agatcaactg tattcacttg tccaatacgg atagacttac
      661 cgcccatcgt aaagctcttc gcatcgcctt caatgatgtc catatccgac ttggcggcta
      721 aattagtgcc cgccttcaac atagccaaac cgtatgattg caggtcaatg ccagcgattt
      781 tagccatttc cggcaagatc tttttgtcct tgtcggtagc agtcggtgac ttcagcaata
      841 acgtatccga aatcaagcca gacgccatta agcctgccag tgtcggtgaa atttcatagc
      901 cgtgttcctg gaacaactcg aaaatcaccg tgttggtact gcccactggt tctgcccggt
      961 aatatagcgg tgacgccgta tggaagttgg caatgcggtg gtgatcaacc actgagtcga
     1021 tttgtacatc ttcaatatca gcaacgcttt gctggaattc gttatgatca accagcatta
     1081 cgtgaccggt ttcgtttgca gctgtcttaa taacccgcgg cgcagcaacc ttaaaatgat
     1141 ccaatacaaa cttggtttca tcgtttggtt caccgagtgc tactgcttca acatcggtat
     1201 tacctgcctg cttctgaaat tcagcccaac tcatggcgct tgtaatcgcg tctgtatcag
     1261 ggttttgatg tccaaaaatc aaagtcgtca tgttatctga tcgctcctca gctgaatagt
     1321 ttaccgttca attttaccac tgttttcgct gacccgcgat agataatctt tctcggataa
     1381 atgcgcgtca aacgtcttaa agaagttggc aatccgcgga atatcagctt tttccttgcg
     1441 atacacaaac gaggtctcaa agcgaatcgc tggttcaaag gcaacggcat gcgccttgat
     1501 ttgatcagca tgagcggcaa caaaactggt tggtaacgcg gtgttaacgc cggtatggtt
     1561 gacaaactga gttagctgat acggcgtggt aaaatgcgcc acaacatgcg gaaaatcaac
     1621 catctgattc ttatatgcct catgcaacaa ctgacccagg aaatagtcgt ctggataagt
     1681 tacccacggc tcagccagcg tatctttgaa atgcaggcgt ttgcgattag ccaaggaatt
     1741 atcatgatgc aggaacaata actgttcatc aagaatcttc ttgctggcat acggcttcca
     1801 gttcttaatc gtctcatcag gcaggttcat gatggccaaa tcaatctggt tgttctccaa
     1861 cgcttcccag atctcatgac gtgtcatcat tcgcaacgtg atttcgactt caggattaag
     1921 atcataataa tgcgcgataa aagcattaaa cgcactgtct tcaattgaac ttaacaagcc
     1981 aatattaatc ttaccgactg tggcactggt actttgctga atctcgtcag tcgtctggtt
     2041 caaaatttca taaatctcgt gtgtcgcctt caacatcgtg cgaccggcat cggataaatg
     2101 caacttctta ccaacggaat aaaacaatgg tgcccccact gttcgttcca atttcttgat
     2161 ttgctgcgtc agtgccggct gggtaattcc caacaactgg gctgactggg tgtagttcat
     2221 ggtttctgct aattgcaaaa aatacgtgag agttttggag gaaaaaatgc gctcttgttt
     2281 cgtcttcata gggcggcttc cctttcgttt ataatttgat aacgttactc aaccatcatc
     2341 ataagtggca tttaaaaaaa acgcaacgga tttgctgcgt ttttaatcgt tacgttaagt
     2401 ttcaactaaa tcactgcttg ttagctggct gcggtcgcca actttgccat ccagtatagt
     2461 cctcaacgtg tagcaagttc tctgcattct tgacccgatc agctgaaggc ggctcaatac
     2521 cttccagcgg atacgtaata cctagttcat ggtacttctt aacccctaac gtatgatacg
     2581 gaagaatttc cactttctga accacgcgtt tatccaaagt ggcaatataa tctcccaagc
     2641 gtttgagata aggatcaaag tcggttcgtt ctggcacaag tacgtggcga atccacatat
     2701 catcgccatg atccgacata tactgaacca tatccaaaat gttttcgttt gggaaaccgg
     2761 tcagcttttt gtgttccgcg gaatcaatgt gcttgatatc aaccaacgaa atatccgtga
     2821 cggccatcag tttctcaaat tgaccaaacc acggctgatc gcgggtgaat ggaccacccg
     2881 aagtatccaa acaagttgag atacccagtt ccttagcttt ggtaaaaagt tccaggataa
     2941 aatcaatttg gacgagactt tcgccaccgc tacaagtaat ccccccttgt tcaccccaga
     3001 aggctttata tcgctgtgca tctgccagga tctgatcagc ggtcatttcc tcgcccacac
     3061 cgatattcca agtgtcaggg ttgtgacagt actggcaccg catcctgcat ccttgcataa
     3121 agacgacaaa ccgaatcccg ggaccatcaa ccgaaccaaa gctttcgact gaatgaacgt
     3181 atccctttat tgggccttca tgatcctgtc ccacttgttc caaaatattt aacgggcttt
     3241 gtgattgatc gatgagtttc atctatgccg cctccagtgc aaataaatac ctaattttca
     3301 acgattttca tttcagtttg tcaaaaaacg gcaacagcat cgttgccgtc ttttgacaaa
     3361 acaggaatga tctcctgttt tgcgaataga ttgacttgat tacatttcgt cgaagaaggt
     3421 ccgagcgata acatcgtctt gctgttcttt ggtcaggtct gcaaagtaga cgcagtaacc
     3481 ggaaactcga acagtcaagg ttggatattc ttctgggtgc ttctgggcat caatcaacgt
     3541 ttctttattg aagacgttga tgttaaggtg catgccgcta ttttccatgt agccgtcaac
     3601 catgtgaacc aaggtgtcct tacgggtttc atcgtcatgg cctaaggtgt tcggtgttac
     3661 cccaaaggta ttgctgatcc catctgttgc atagtgatat gggatcttgg cggttgacat
     3721 caaggaagct aaagcaccgt tcttttcagc gccatacgct gggttggcac caggtgagaa
     3781 tggttcgccc ttctgacggc cgtttggtgt tgtcccggta ttcttaccat aaaccacatt
     3841 tgatgtaatg gtcagaacac tggtactgag cttagcaccg cgatacagat gatgtgtatt
     3901 catcttgttg tagaaggtct taaccaacca cttagcaatg ttgtctgccc ggtcatcatt
     3961 gttaccataa cgcggatagt catcattgtc agcaacaaag tcgattgcaa cgccattttc
     4021 atcccgaatt gccttcacat ggccatactt gatggcactg atggaatcaa ccgcatgtga
     4081 caaaccggaa atcccggtag caaacgtccg attcagacgc gtgtctttca atgccaactg
     4141 tgcagcttca tagtagtact tgtcatgcat gtagtgaata acattcagtg cattgacata
     4201 gacatctgct aaccagtcca tcatcttgtc gaacttcttc atgaactcgt catagtcgat
     4261 atagtcgctg gtgatcggct cgtattcagg tcccacctgt gcttcctgaa tttcgtcttt
     4321 accaccgttg atcgcataca gcacggtctt ggctaggttt gctcgcgccc cgaagtactg
     4381 aacgccatct gcaattggct gtgcggaaac acagcaagca atgccgtaat agtcggttcc
     4441 ccattcgttg cgcatcagat catcgttttc gtactgaatg gttgaacttt gaatggacac
     4501 ttcagtagca taacgtttga acccttcagg aagacgttcg ctccacaaca aggtgatgtt
     4561 tggttccggt gccgcgccca tgttgtccaa ggtcttcagg aagcggaacg cggtcttggt
     4621 aacatgatga cggccatcat tacccatccc tgccaaggac agtgttgccc agatcggatc
     4681 gccggagaac agtgagttgt attcttcagt ccgaatgaaa cgaaccatcc gcaacttcat
     4741 caccaaatga tcgatgagtt cttgtgcctg ctcttcattc agcttgccca aacgcatatc
     4801 ccgttgaata taggcatcaa tcactgtatc gatacggccg acagacattg ccgcaccatt
     4861 ttgggtctta acggctgcca ggtaaccgaa gtagatccac tgaacagctt cttgagcatt
     4921 agcagctggg cgggaaatgt catagccgta ttctttggcc atcttcttca tgtcatt
//
