LOCUS       gnlProkkaNOEMIMDN_56   11395 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NOEMIMDN_56.
ACCESSION   gnlProkkaNOEMIMDN_56
VERSION     gnlProkkaNOEMIMDN_56
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 00:23:48
            Original ID  :: gnl|Prokka|NOEMIMDN_56
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11395
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..11395
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[1336:6395]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1337..6395
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..11395
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..11395
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             40..1323
                     /locus_tag="ctg47_1"
                     /transl_table=11
                     /translation="MIRSFLSRSPLYVLALYASVLIFLVYTCAYAYRKPFTAAIYEGEI
                     LWGFDVKILYVLSEIIGYALSKFIGVRILPSMKAGHRIYYIIGLLTFSEVALLGFALLP
                     VPLKVCSIFLSGLPLGMIWGVIFSYIEGRRISEILNVGLSVALIVSSGLVKTLGQFVMD
                     NLHVSEYWMPFTTGLLVYPVMLLCSWLLNQIPAPNEQDIIQRTKRAPMDGKDRRKFLRQ
                     FFWGICMLVVFYGALTVFRELRDSFAADIWKELHIEGAMIFTQTEVPIAVFVLVLMFLI
                     VFVRNNRLALNIIYCIAVTGGSLMVFSTLLYVYGFLSPIWWMILSGLGLYMGYIPFTYL
                     IERLIASLRVVSTAVFIIYLADSFGYLGTTGVFMVKNFVSADISWTVMLMRTALFSGFV
                     SVLSIFAIYCYFKQQLNSLILIPSEIHD"
     CDS             1337..2035
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg47_2"
                     /sec_met_domain="NTP_transf_3 (E-value: 2.2e-14, bitscore:
                     44.4, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAGLGTRFGHYTELVPKGFVEVGGKPMIIRSIETLLSCGIERII
                     LGTGYKKEAYEALQADFPQIETCFSPRYADTNSMYTLYNTRDVIGDDSFLLLESDLVFE
                     RKAILSLLDDEFPDVMLVSSLTKFQDQYYVEYDRNHILTSCSVDKNALEAKGELVGIHK
                     LSNTFYRRMCADYATILESQPKLGYEYELLRMSRSVSPVRVLRVEGLKWYEIDDEADLS
                     YAEEHIIRYC"
     CDS             1974..3356
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 213.9;
                     E-value: 4.7e-65)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg47_3"
                     /sec_met_domain="CTP_transf_like (E-value: 2.7e-12,
                     bitscore: 37.4, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 2.1e-122, bitscore:
                     398.0, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 1e-64, bitscore:
                     208.6, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSMMKPTFRMPRSTSSAIVKNKSFPYMNKKIYVGMTADIMHPGL
                     IHIIHEATKYGDVIVGLLTDKAIAEHKRLPYLTYDQRKEVVENIKGVFEVVPQEDWSYV
                     PNLEKLKPDYIIHGDDWKTGPLREVREQVFEVMNAQGGKVIEIPYTRGINSSSLDKDIK
                     AIGTTPDVRLKSLRRLIQAKPVVRILEAHDGLCGLIIENLEVQKGDKREVFDGMWSSSL
                     TDSTSKGKPDIEAVDLTTRLQDLNNILECTTKPIIFDGDTGGKIEHFVFTVRTLERHGI
                     SAVIIEDKVGLKKNSLFGTDAIQTQDSIEGFCDKIRAGKNAQVTGDFMIIARIESLIAG
                     KPMSDALERAYAYVQAGADGIMIHSKDKSGEDIREFCRTFRKEYAHVPIVVVPTTYDHV
                     HESELHEWGANVVIYANHLLRAAYPAMMNVARTILENERAEEVRPLCMPIKEILELIPG
                     TK"
     CDS             3368..4504
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 71.8;
                     E-value: 7.9e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg47_4"
                     /sec_met_domain="TPP_enzyme_N (E-value: 2.9e-22, bitscore:
                     69.4, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 7.6e-21, bitscore:
                     64.8, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVSVEKIYDTFLHHGVDFFTGVPDSLLKNICAYITDHAPRGKHII
                     AANEGSAVGIACGYYMASGNVPLVYMQNSGLGNTVNPLLSLADEKVYSLPLLLLVGWRG
                     EPGTKDEPQHKKQGEVTLDLLKAMKIPFIILDTDEDEALAQIHDAVVSAKSRSIPHAIV
                     IRKDTFGKYKLRQEALNDYPLSREEALMQVVDHLGDNDIVVSTTGKLSRELFEYRESKG
                     QGHARDFLTVGSMGHSSSIALGVALEKPDRRVYCLDGDGAFIMHMGAVSNIGDLSPKNY
                     RHVLFNNGAHESVGGQPTLGFRLDIASIAKGCGYAHVLTASDKEGLSCALEKLSGLSGP
                     VLLEIKVRIDSRDDLGRPTTTPVENKEHFMAFLKDTHS"
     CDS             4501..5619
                     /NRPS_PKS="Domain: Aminotran_5 (30-299). E-value: 6.2e-34.
                     Score: 109.3. Matches aSDomain:
                     nrpspksdomains_ctg47_5_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 80.5; E-value:
                     2e-24)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg47_5"
                     /sec_met_domain="Aminotran_5 (E-value: 1.5e-31, bitscore:
                     99.9, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIERKILLNPGPATTTDTVKLAQVVPDICPREKEFAGMMKQLRD
                     DLVRVAHGDLSKHTAVLFCGSGTINIDICLNSLLPADKKVLVVNNGAYSTRAVEVCEYY
                     GLPHIDLKFSVYELPDLSVIEKTLSDNPDIALVYTTHHETGTGILNPIREIGKIAHSHG
                     AVFVVDTTSSLGMIPFDIGKDNVDFCMASAQKGLQAMTGLSFIIGDESLIRASRDYPKR
                     SYYCNLYLQYDYFEKTGEMHFTPPVQTVYAARQALDEYFAVGEEAKFARHKRVFEAIHK
                     GLQELGLQYAIKREWQSGLVVSVLYPDDPLWDFDKVHDYCYERGFTIYPGKISTTNTFR
                     LCALGEIDVEDIERFFEVMREAFSLIKNITNL"
     aSDomain        4591..5397
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg47_5_Aminotran_5.1"
                     /evalue="6.20E-34"
                     /label="ctg47_5_Aminotran_5.1"
                     /locus_tag="ctg47_5"
                     /protein_end="299"
                     /protein_start="30"
                     /score="109.3"
                     /tool="antismash"
                     /translation="PREKEFAGMMKQLRDDLVRVAHGDLSKHTAVLFCGSGTINIDICL
                     NSLLPADKKVLVVNNGAYSTRAVEVCEYYGLPHIDLKFSVYELPDLSVIEKTLSDNPDI
                     ALVYTTHHETGTGILNPIREIGKIAHSHGAVFVVDTTSSLGMIPFDIGKDNVDFCMASA
                     QKGLQAMTGLSFIIGDESLIRASRDYPKRSYYCNLYLQYDYFEKTGEMHFTPPVQTVYA
                     ARQALDEYFAVGEEAKFARHKRVFEAIHKGLQELGLQYAIKREWQSG"
     CDS             5616..6395
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 151.1; E-value:
                     6.3e-46)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg47_6"
                     /sec_met_domain="NTP_transf_3 (E-value: 1.7e-11, bitscore:
                     35.0, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAVILAGGFGSRLSEATNLIPKPMVEIGGKPILWHIMKIYSYYG
                     INEFIICCGYKQYVIKEYFANYFRHNCDMTVDLSNNSTTILDNHSENWKVTMIDTGVNT
                     MTGGRVKRIQKYVGNECFLLTYGDGVADLDISDTIKVHKESKAILSLTAYKPHGKFGLL
                     DISSETNMVKSFLEKPDGDGNWINAGFFVCEPKVFDYIPANDDSIVFEKQPLESIARDG
                     KMYAYKHCGFWKPMDMLRDNIELNEMWNNGKAPWKVW"
     CDS             6399..7475
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     137.4; E-value: 1.1e-41)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg47_7"
                     /sec_met_domain="RmlD_sub_bind (E-value: 7.4e-11, bitscore:
                     31.9, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 9.9e-16,
                     bitscore: 47.9, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVDVFNNFYRGKRVLVTGHTGFKGSWLSIWLYELGAEVVGVSLD
                     PITERDNYVLSGIGQKIKADIRADICDGESMKGIFQEYQPEIVFHLAAQPLVRLSYNIP
                     VETYETNVMGTIHILEAIRVTNSVKVGVMITTDKCYENKEQIWGYRENEPMGGYDPYSS
                     SKGAAEIAIASWRRSFFHPKQYKKHGKSIASVRAGNVIGGGDWALDRIIPDCIKALEAG
                     RTVDIRSPKAIRPWQHVLEPLSGYMLLAQKMWDEPTQYCEGWNFGPRAESISTVWDVAS
                     RVVESFGCGELRDLSDPNALHEADLLMLDISKVKFQLGWEPRMNIDQTVRLTVDWYRRY
                     QKEEMYKVCVEQIEKYIR"
     CDS             7505..8383
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score: 60.1;
                     E-value: 3.9e-18)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg47_8"
                     /transl_table=11
                     /translation="MLSNSIGTVLKKVGYFIQVIGKSSPVAFAPDVFIRKNLLEDAVYI
                     TELLDSDVIIYAAGAGVQAAVPIAANTMYTMNLLVPIKLCLLLNEHNYLGTFVSFGTYM
                     EIGCNDEMYKSFNEDELLHSCLPVTNDYVLSKRLFTHYIHNANFVFRKYHFVLPNLFSR
                     DEVGTRLLPYVVNYLRERKKCNKVEQPRFSSGSQIRQYVFIEEVIEAIFKCIEGEVVSG
                     IYNIGGGQTATIREFIEELFNAFCVDVDEHMFGKELRRDNDILSLSLNDAKLYKAIHYS
                     PIIKITDIYHE"
     CDS             8376..8789
                     /locus_tag="ctg47_9"
                     /transl_table=11
                     /translation="MSSINIESKIKIISRRKISDNRGWFLKIIDGKENSLPNYTGEIYI
                     VSAISGESRGGHYHNRAIEWFTLVEGNAVLKLEDIRTNEKKEIYLNDQEPVTVFVPPHI
                     AHEFINIDSVPFIVIAYTDQLYKPEDTIQYILK"
     CDS             8793..9716
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg47_10"
                     /sec_met_domain="Glycos_transf_2 (E-value: 3.9e-25,
                     bitscore: 79.0, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTFSLFLITRNRPHLLKLALNSVLNQTISNFPIIVSDNSTNNET
                     KEMVESLYLNQSNIIYRKREREYGLIEHWNKVLSEVRSEYFMIFHDDDVMHPDMIRTML
                     NIIKKDHSLAFIATNARIIKKGRICYSKYKGKEKIMRLKEARELFQMYAVGNWIPPFPS
                     YIYSKSIADNMRFAEDKGGKYCDVAFLLDAFDLGNLAIITKPYMDYYYHDGQVSQFNDY
                     FQRIKLINYVRQKMRISPHDRPLVNFKLMNLYCWINNLVRNSDDLVSKKRFINCLKIFL
                     KHRKYMYVFKLLGCIILREKMKCGKL"
     CDS             9701..10708
                     /locus_tag="ctg47_11"
                     /transl_table=11
                     /translation="MRQIVTCTGHGGTGSSFITDFFKEFENVKSTGDFEFTLSYDVDGI
                     YDLYHNISHYSGFHIAESFDRFDKLCSDIRKSYKNTLGCDIYPLLKDYISAITEVDWNG
                     FWPEHVRREPYIKKILLYYLPNIIQRNLNKIVKFNGDYEYVDRTIRQSMRLSVDSSLFL
                     QETRKLYNKIFDIVDDSNECTHLVLDRLVPPTNIEDYMIFIDNLKVVVVDRDPRDLYLL
                     NKKYWHEGWIPEDVDKFIKLYRFHRLRDKERDLIEDHSSILRVKFEDGIFDYDNTSAQL
                     CDFVGVNRGRHTKKMQFFNPDISRKNVQLWKYEKCYFADVLKIEKELTEFLYIV"
ORIGIN
        1 tttggaagct aatccatatc cgtacagcga actacacgaa tgattcgttc ctttctttcc
       61 cgttctcccc tttacgtttt ggctctctat gccagcgttt tgatttttct ggtttatact
      121 tgtgcctatg cctaccgtaa gccatttacc gcagccattt atgaaggtga gattctttgg
      181 ggattcgatg tcaaaatcct gtatgtcctt tctgagatta tcggctacgc cctttccaag
      241 ttcatcggtg ttcgtattct gccttcgatg aaagcgggtc accgcatcta ttatataata
      301 gggctgctta ctttttccga agttgctttg ttgggttttg cacttcttcc ggttcctttg
      361 aaggtgtgtt ctatttttct gagcggtctt cccctgggta tgatctgggg tgtcattttt
      421 agctatatcg aaggacgtcg tatcagtgaa atattgaatg tcgggctgag cgttgctttg
      481 atagtctctt ccggtttggt gaaaaccttg ggacaattcg tgatggataa tttgcatgtg
      541 tcggaatatt ggatgccttt taccaccggt ttgttggtat accctgttat gttgctttgc
      601 agctggcttc tcaatcagat tccggctccc aatgagcaag atatcataca gcgtaccaaa
      661 cgtgctccga tggatggtaa agacaggcgt aaatttttga ggcaattctt ctggggaatc
      721 tgcatgctgg ttgtttttta cggtgccctg accgtgttcc gtgagcttcg tgactctttc
      781 gctgccgata tttggaagga gctccatatt gaaggtgcaa tgatctttac tcagacagag
      841 gttcccattg ccgtttttgt ccttgtactg atgttcctga ttgtgtttgt ccgtaataac
      901 cgtttggctc ttaatatcat ttattgcatt gccgttacag gcggttcctt aatggttttt
      961 tccacattgc tttatgtgta cggttttctt tctcctattt ggtggatgat tctttccggt
     1021 cttggtctgt atatggggta tattcctttc acttacctga tagaacgcct gattgcttcc
     1081 cttagggtgg ttagtacggc tgtttttatt atttatttgg ccgattcttt cggctatctg
     1141 ggcactacgg gtgtgtttat ggtcaagaac ttcgtttctg ctgacatttc ttggactgtc
     1201 atgctaatgc gtactgccct tttttcagga tttgtttctg tcctgtcgat ttttgcgatt
     1261 tactgttatt tcaagcaaca attgaattca cttatattaa ttccctctga aattcatgat
     1321 taaaacagca atgatcatgg ctgccggtct tggtacccgc ttcgggcatt ataccgagct
     1381 tgtgcccaaa ggttttgtgg aggttggtgg aaaacctatg attatccgct ctatcgaaac
     1441 ccttctttcc tgcggtatcg aacgtatcat cctcggtacg ggttataaaa aagaggctta
     1501 tgaagccctt caggcggatt tcccccagat agagacctgt ttcagtcccc gttatgccga
     1561 tactaacagt atgtacactc tgtacaatac gcgtgacgtg ataggcgatg acagtttcct
     1621 gctgcttgaa tcggaccttg tttttgaacg caaggccatc ctctcgctac tggatgacga
     1681 attcccggat gtgatgcttg tctcttctct gaccaagttc caggatcagt attatgtgga
     1741 atatgaccgc aatcacatcc ttacttcctg ttcggtagac aagaacgccc ttgaggcaaa
     1801 aggcgagctg gtcggtatcc ataaactttc caatactttc taccgccgga tgtgcgccga
     1861 ttatgcgacc atcctggagt cccagcctaa actgggttac gagtacgaac tgctccgcat
     1921 gtcccgttcc gtttcccctg tccgtgtgct cagggtggag ggtcttaaat ggtatgaaat
     1981 cgatgatgaa gccgaccttt cgtatgccga ggagcacatc atccgctatt gttaaaaaca
     2041 aatcttttcc atatatgaac aagaaaatct atgtcggtat gaccgcggat atcatgcatc
     2101 cggggctgat ccatattatt catgaagcta ctaaatacgg tgatgtcatc gtcggtctgc
     2161 tgacggacaa ggccatcgcc gaacacaaac gcctccctta ccttacctat gatcagcgta
     2221 aggaggttgt tgagaatatc aagggggttt ttgaggttgt tccccaggag gactggagct
     2281 atgttccgaa tctggaaaaa ctcaagcctg actatatcat ccacggcgac gactggaaaa
     2341 cgggcccgtt gcgcgaggtg cgcgaacagg tttttgaggt gatgaacgcc caggggggca
     2401 aggtaatcga gatcccttat acccggggca tcaactcctc ctcgctcgac aaggatatca
     2461 aggcgatcgg gactacccct gacgtacgtc ttaagtccct ccgccgcctg atccaggcca
     2521 agcctgtggt ccgtatcctg gaggctcacg acggtctttg cggtctgatt attgagaatc
     2581 ttgaagtcca gaagggtgat aagcgcgagg tctttgacgg catgtggtcc agcagcctga
     2641 ccgactccac gagtaagggt aaaccggata ttgaagcggt cgaccttacc acccgtttac
     2701 aggacctgaa caatatcctt gaatgtacca ccaagcctat tatttttgat ggagacacgg
     2761 gtggcaagat cgaacacttt gtctttactg tgcgcactct ggaacgtcac ggcatttctg
     2821 cggtaatcat tgaagataaa gtcggtctga agaagaattc cctttttggt actgacgcta
     2881 tccagactca ggatagtatc gagggtttct gcgataagat cagagcaggg aagaatgccc
     2941 aggtcaccgg tgactttatg atcattgcgc gtatcgaaag ccttattgcc ggcaaaccca
     3001 tgagtgacgc cctcgaacgt gcttatgcct atgtacaggc gggtgcggac ggcatcatga
     3061 tccacagcaa ggataagtcg ggtgaggata tccgtgagtt ctgccggact ttccgcaaag
     3121 aatacgccca tgttcccatt gtcgttgttc cgacgactta tgaccatgtg cacgaatccg
     3181 aacttcacga atggggtgcc aacgtagtca tttacgccaa ccaccttctc cgtgccgctt
     3241 acccggccat gatgaacgtg gccaggacca tccttgaaaa cgagcgcgcc gaggaggtcc
     3301 gtccgctttg tatgcctatc aaggaaattc tggaattgat tccgggcact aaataaatca
     3361 gacggttatg gtaagtgtag aaaagatata cgatacgttc cttcatcacg gtgttgactt
     3421 ctttacgggt gttcccgatt cccttttgaa gaatatttgc gcctatatca cggatcatgc
     3481 tccgcgtggt aagcatatca tcgccgccaa tgaaggttcc gcggtgggca ttgcctgcgg
     3541 ttattatatg gcttctggca acgttccttt ggtttatatg cagaactccg gtctgggaaa
     3601 tacggtgaat cccctcttgt cccttgcgga cgagaaagtg tacagccttc ctcttctttt
     3661 gctggtcggc tggcgcggtg agcccggcac aaaagacgaa ccccagcata agaagcaggg
     3721 cgaggttacc cttgatcttc tgaaagccat gaaaattcct tttataattc tggatacgga
     3781 tgaagacgag gccctggcgc agatccatga cgcggttgtc tcggcgaaga gccggtcgat
     3841 tccccatgcg attgtcatcc gtaaagatac gtttggaaaa tataaattgc gtcaggaagc
     3901 cttgaatgac tatcctctgt cccgcgagga ggcgctaatg caggttgtcg accacctcgg
     3961 ggacaatgac attgtggttt ccaccacggg caagctctcc cgtgagcttt tcgagtaccg
     4021 tgaatcgaaa gggcaggggc acgcaaggga tttcctaact gtgggttcca tggggcacag
     4081 ttcttccatt gccctggggg ttgcccttga gaagcctgac cgccgtgtct actgtcttga
     4141 cggtgacggt gcctttatca tgcacatggg tgctgtcagc aatatcggcg atctttctcc
     4201 caagaattac aggcacgtcc ttttcaacaa cggtgcccat gaatctgtcg gcggtcaacc
     4261 cacgctcggt ttccgtctgg atatcgcctc gatagcgaaa ggttgcggtt atgcgcatgt
     4321 actgactgcc tccgacaagg aagggctttc gtgtgcgctt gagaagctgt ccggcctgtc
     4381 cggtccggtt cttcttgaaa ttaaggtccg gatcgattca cgtgatgatc tgggtcgccc
     4441 tacgactact ccggtagaga ataaagagca ttttatggca tttttaaaag atacgcattc
     4501 atgaatatcg aaagaaagat ccttttgaat ccgggtcccg ccaccacgac ggatacggtg
     4561 aagctggcgc aggttgttcc cgatatctgt ccgcgtgaga aagagtttgc gggtatgatg
     4621 aaacagttac gtgacgacct ggtccgcgtt gcgcatggag acctctccaa acatacggcc
     4681 gtcctgtttt gcggctcggg tacgatcaat atcgatatct gcctgaactc tcttcttcct
     4741 gcggataaga aagttctggt tgtcaataac ggtgcctaca gcacccgtgc ggtggaagtc
     4801 tgcgaatatt acggtcttcc ccatatcgat ttgaagtttt ccgtttacga acttcccgac
     4861 ctgtcggtaa tcgaaaagac gctttccgac aatccggaca tcgctttggt ctataccacg
     4921 caccatgaaa cgggtacggg tatcttgaac cccatccgtg agatcggaaa aatagcccat
     4981 tcgcacggtg ccgtttttgt tgtcgacacc acttcctccc tgggtatgat tccttttgat
     5041 atcggaaagg ataatgttga cttttgtatg gcttcggctc agaaaggcct tcaggccatg
     5101 acggggcttt cctttatcat aggtgatgaa tccctgatac gtgcttcaag ggattatccg
     5161 aaacgctcct actattgtaa cctctattta caatacgatt actttgagaa gacgggtgag
     5221 atgcatttta ctcctcctgt tcaaaccgtt tacgctgccc gtcaggctct tgatgaatat
     5281 tttgcagtag gtgaagaggc taaattcgcc cgtcacaaga gggtctttga agccatccat
     5341 aaaggtttgc aagagcttgg tcttcagtat gcgatcaaac gtgaatggca gtcgggtctt
     5401 gttgtttctg tattgtatcc tgatgatccc ctctgggatt ttgataaggt tcatgattac
     5461 tgctatgaac gtggctttac gatctacccg ggtaagattt ccacaacgaa tacattccgg
     5521 ctttgtgcgt tgggagagat tgatgtggag gatattgaga ggttctttga ggtgatgaga
     5581 gaggcatttt ctttaattaa aaatattact aatctatgaa agctgttatt ttagcaggag
     5641 ggttcggatc tcgtttaagt gaggctacta atttgatacc taagccaatg gtggaaattg
     5701 ggggaaagcc aatactttgg catattatga aaatttatag ttattatggt attaatgaat
     5761 ttattatttg ctgtggctat aaacaatatg tcataaaaga atattttgca aactattttc
     5821 gtcataattg tgatatgaca gttgatctat ctaataatag cactactatt ttagataatc
     5881 attcagagaa ctggaaagta actatgatag atactggggt gaatacaatg actggaggac
     5941 gtgtaaaacg catccagaaa tatgtaggaa atgaatgttt tttgttgact tatggtgatg
     6001 gtgttgcaga tcttgatata tctgatacga taaaagtgca caaagaatca aaagcaattc
     6061 tttctcttac agcttataaa cctcacggaa aatttggctt attagatata tctagtgaga
     6121 cgaatatggt gaaatcattc ctggaaaaac cggatggtga tggaaactgg ataaatgcag
     6181 gctttttcgt ttgtgaacca aaagtctttg attatatacc tgctaatgat gattctatcg
     6241 tctttgaaaa acaaccgtta gaatcgattg cccgcgatgg taaaatgtat gcttataaac
     6301 actgtggctt ttggaagccg atggatatgt taagggataa tatagaactg aatgaaatgt
     6361 ggaataatgg gaaagcccct tggaaagttt ggtagtatat gagtgtagat gtatttaata
     6421 acttctatag aggaaaacgg gttcttgtta ccggccatac tggttttaaa ggttcatggc
     6481 tttctatctg gttatacgaa ttaggtgctg aagtggtagg tgtcagtttg gatcctatta
     6541 cagaacgaga taactatgtt ctttctggta taggacaaaa aattaaggcg gatattcgtg
     6601 ccgatatttg tgatggcgaa agtatgaaag gtatctttca agaataccaa cctgaaatag
     6661 tcttccattt ggcagcccaa ccattggtac gtctcagtta taatattcct gttgaaacat
     6721 atgaaacaaa tgtaatggga acgattcata tacttgaagc cattcgcgta acaaatagtg
     6781 ttaaagtagg agtaatgata actacggaca aatgttacga gaataaagaa caaatttggg
     6841 gatatcgtga aaatgaaccg atggggggat acgatcctta ttcaagtagt aaaggagctg
     6901 ctgaaatagc gattgcttcc tggcgtcgct ctttctttca tcctaaacag tacaagaagc
     6961 atggtaaaag catcgctagt gtacgcgcag gaaatgtaat tggtggtggt gattgggcat
     7021 tagaccgcat aattccagat tgtataaaag ctttagaagc aggaaggact gttgatattc
     7081 gaagtcctaa agcaattcgc ccttggcaac atgtacttga acctctgagt ggatatatgc
     7141 ttcttgcaca gaaaatgtgg gatgagccga ctcaatattg tgaaggttgg aattttggtc
     7201 ctagagcaga atctatttct accgtatggg atgttgctag tagggtcgtg gagagttttg
     7261 gatgtggtga attaagggat ttgtctgacc cgaatgcttt acatgaggct gatttattaa
     7321 tgctggatat ttctaaagta aaattccaac tgggctggga gccaagaatg aatattgatc
     7381 aaacagtaag attgactgta gattggtata gaagatatca aaaagaagaa atgtataaag
     7441 tttgtgtgga gcaaatagaa aagtatatcc gatgaaaata actattatag gaggaggaag
     7501 tggaatgctt tcgaatagca ttggaactgt tttgaaaaaa gttggctatt ttattcaggt
     7561 tatcggtaag agttctcctg tagcatttgc tcctgatgta tttataagaa aaaatctttt
     7621 agaggatgcc gtttatatta cggagttatt agattcagat gttattatat atgcagcggg
     7681 agcgggtgta caagcagcag tgccaatagc ggcaaataca atgtatacaa tgaatttgct
     7741 ggtacctata aaactctgtt tattattgaa tgagcataat tatctgggga cttttgtatc
     7801 ttttggaact tatatggaga ttggatgtaa tgatgaaatg tataaatcat ttaatgaaga
     7861 tgaacttttg cattcttgtc ttcctgtaac taatgattat gtcctttcta aacggctttt
     7921 tacacattat atacataatg caaatttcgt ttttagaaaa tatcactttg ttttacctaa
     7981 tctcttctca agagatgaag ttggaacaag attgttacct tatgttgtta attatctgag
     8041 agagagaaaa aaatgtaata aagttgagca acctcggttt tcttctggtt ctcagattcg
     8101 acagtatgtg tttatagaag aagtgataga ggctattttt aaatgtattg aaggtgaagt
     8161 agtaagtgga atatacaata ttggtggtgg tcagacagct acaataaggg aattcataga
     8221 agaacttttc aatgcttttt gtgtagatgt agatgaacac atgtttggaa aagaattaag
     8281 aagagacaat gatatattat cactctcatt gaatgatgcc aaactttaca aggctataca
     8341 ttattctcct ataattaaga taacagatat atatcatgag tagtataaat atagaatcca
     8401 aaatcaagat aatctctcga agaaaaattt cagataatag gggatggttt ttaaagataa
     8461 ttgatggaaa agaaaattca ttacctaatt atacaggtga aatatacatc gtgagtgcca
     8521 tatcagggga atctcgtgga ggacattatc ataatagagc gattgaatgg tttactttgg
     8581 ttgagggaaa cgctgtttta aaacttgaag atattcggac taatgaaaaa aaggaaattt
     8641 atttgaatga tcaagagcct gttaccgtgt ttgtccctcc acatatagca catgagttta
     8701 taaatataga tagtgttcca tttattgtga ttgcatacac tgatcagtta tacaaaccag
     8761 aagatacaat ccaatatata ctgaaataag atatggatac tttttcattg tttttgatta
     8821 ctcgtaatcg tcctcattta ttaaaactgg cattaaattc tgtactaaat cagaccatct
     8881 ctaattttcc aatcattgtt tctgataact ctacaaacaa tgaaacgaaa gaaatggtag
     8941 aatctctcta tttaaatcaa tctaatatta tttatcggaa aagagaaaga gaatatggtt
     9001 taatagaaca ctggaataaa gttttgtcag aagtccgtag tgaatatttt atgatatttc
     9061 atgatgatga tgtaatgcat cctgatatga ttaggacaat gcttaacata ataaaaaagg
     9121 atcattcatt agcttttatt gccacaaatg caagaattat aaaaaaaggt agaatatgtt
     9181 attctaaata taagggaaaa gagaaaataa tgcgattgaa agaagctaga gaactttttc
     9241 agatgtatgc agtgggaaat tggattcctc cctttccgtc ttacatatat tcaaaatcta
     9301 ttgctgataa tatgagattt gcagaagata aaggtggaaa atattgtgat gttgcttttc
     9361 ttttagatgc ttttgatctg gggaatttag ctattattac aaaaccatat atggattatt
     9421 attatcatga tgggcaagta agccaattta acgattattt tcaaagaata aaattgataa
     9481 attatgtacg tcaaaagatg agaatatctc ctcacgatcg tcctttagtt aattttaaat
     9541 taatgaatct ttattgttgg attaataatt tggttagaaa ttccgatgat ttagtttcga
     9601 aaaagagatt catcaattgt cttaaaatct ttcttaaaca tagaaagtat atgtatgttt
     9661 tcaaattgtt agggtgtatt attcttagag aaaaaatgaa atgcggcaaa ttgtaacttg
     9721 tacaggacat ggaggaacag gctcttcctt tattacagat ttttttaaag aatttgaaaa
     9781 tgtaaaatca acaggagatt ttgagtttac attgagttat gacgttgatg gtatttatga
     9841 tttatatcat aatataagtc attatagcgg tttccatata gctgaatctt ttgatcgttt
     9901 tgataaattg tgtagtgata ttcgtaaatc gtataaaaat acattgggat gtgatatata
     9961 tcctttgtta aaagattata tctctgcaat tacagaggtt gactggaatg gattttggcc
    10021 tgagcatgta aggcgtgaac catatataaa aaaaattctt ttgtattatc ttcctaacat
    10081 aatacagaga aatctaaata aaattgtaaa atttaatggt gattatgaat atgttgatag
    10141 aacaattagg cagtctatga gactttctgt tgattcatcg ttgtttttac aggaaacaag
    10201 aaagttatat aataaaattt ttgatattgt tgatgattct aatgaatgta cacatctggt
    10261 attagacaga ctggtacctc ctacaaatat agaagattac atgatattca ttgataattt
    10321 gaaggttgtg gttgtggata gagaccccag agatttatac ctattgaata agaaatattg
    10381 gcatgaagga tggattccag aagatgttga taaatttatt aagctatatc gtttccatcg
    10441 tttaagagat aaagaaagag acttaataga agatcattcg tctattttaa gagtgaaatt
    10501 tgaggatggt atttttgatt atgataatac aagtgcccaa ttatgtgatt ttgtaggagt
    10561 aaatagagga aggcatacaa agaaaatgca gttttttaat ccggatattt cgagaaaaaa
    10621 tgttcaatta tggaaatatg agaaatgtta ttttgcagat gttctgaaga tagaaaaaga
    10681 gttaacggaa tttttatata tagtttagag ttatggaaca gatttggaaa gttgcagtat
    10741 atagtgctac tacccaccct ttaataaaac attttcccaa gcaaattcct aaatggggga
    10801 attgtgagtt tatcttaaac tcgggtagtg aagactgtga ttgtttattt gtcattgatt
    10861 ctattttagg ggatattcct gttaaatgtg gaaaagaaaa tactttttta tgtatttgtg
    10921 agccggcttc tgttaagtta tatagctcta aatatattaa tcaatttggt aatattgttt
    10981 catttagaaa tttttctcgt tataaaggaa ataccatttc ttcatttcca ttattgccgt
    11041 ggatggtagg ctctaaattt gattttgatt taaaagcttg ggataaagat aatttcttag
    11101 attatgatac tttacaacaa agagtaactt gtgataaaaa gaataaagtt gcggtgatta
    11161 cttcaaataa aacattgact cgaggacatc gtcagcgatt ggattttata ttgaaattaa
    11221 aatcaataat accagatctt ttggatattt atggtgaagg ctttgatttt gttatagata
    11281 aatatgatat ctattcaaaa tataagtatg tgttggcttt cgagaactgt cgagaaaaag
    11341 gatattggac cgagaagatt gctgatgcct atttatgtga agctttccct tttta
//
