LOCUS       c00203_gnlPro..         3264 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DOOGDFOH_310.
ACCESSION   c00203_gnlPro..
VERSION     c00203_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 22:26:50
            Original ID  :: gnl|Prokka|DOOGDFOH_310
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3264
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3264
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[215:3174]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      216..3174
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3264
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3264
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..98)
                     /locus_tag="ctg203_1"
                     /transl_table=11
                     /translation="MGRSQRAVITSKYTFLIFFCGIICKLKNDSYL"
     CDS             216..2444
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg203_2"
                     /sec_met_domain="Gly_radical (E-value: 1.1e-47, bitscore:
                     148.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1166.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFKDGLWSKEINVRDFVSNNITPYKGDASFLQGPTERTKA
                     VWNHCLKALEEERNNNGIRALDYTTVSTITSHPAGYIDKENELIVGLQTDQVLKRAIKP
                     FGGINVVMKACRENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGLDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKEIK
                     VLGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSQ
                     GTITETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESIGGRFNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKAFCAQVSIDTSSVQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKDIPVLTGETL
                     KFEEVMANYKKVLIQIARVYNEAMNIIHYMHDKYYYEKAQMAFVDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAHVKARRNDIGLTEGFDIEGAFPCFGNDDDRVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGIAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATQEDRIDNLVTMMDGYFTKGAHHLNVNVLN
                     REMLQDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2449..3174
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.3;
                     E-value: 1.6e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg203_3"
                     /sec_met_domain="Radical_SAM (E-value: 1.5e-31, bitscore:
                     97.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIEAKGG
                     TATDPEEIVRMAVSQKAFFGKKGGITFSGGEPTFQAKSLIPLFKRLKEAGIHICLDTNG
                     GLWNNDVEELLELTDLVLLDIKEFNPEHHQSLTGRSNEQTLKTAAWLETNHKPFWLRYV
                     LVPGYSDFEDDIRQLGEHLGTYQMIQRVEILPYHTLGVHKYEAMNKEYMLKGVKENTPE
                     QIEKAEKLFRQYFRTVQVN"
ORIGIN
        1 caaaggtacg aatcattttt taacttgcaa attattccgc aaaaaaatat tagaaaagta
       61 tatttggaag taataaccgc cctctggctt ctccccataa ataggtattt ttatgctatt
      121 caacataaaa taggaccctt ttatatacct aaaccgaaaa aaggttgtaa atttgcgcgc
      181 tctaaaggat aactaaccac taactgatta attttatgga actgaataag acctttaaag
      241 atggactttg gagcaaagaa atcaatgtaa gggatttcgt tagtaacaac atcactcctt
      301 ataaagggga tgcttctttc ctgcaaggac ctaccgaacg caccaaagct gtctggaatc
      361 actgtctcaa agctctcgaa gaagagagaa acaataatgg tattcgtgca ctcgactaca
      421 caactgtatc tactataacc tctcaccctg ccggctacat cgataaagag aacgaactga
      481 tcgtcggact acagaccgat caggtgttaa aacgtgccat caaaccgttt ggcggaatca
      541 atgtggttat gaaagcttgc cgtgaaaacg gagtggaagt agatgacaga gtcaaggata
      601 tcttcactca ctatcgcaag actcataatg atggggtatt cgatgtgtat accgaagaaa
      661 ttcgttcgtt ccgttcattg ggattcctta ccggacttcc cgacaactat gcacgcggac
      721 gtatcatcgg tgattatcgc cgcctggctc tctatggtct tgaccgtctg atagaagcta
      781 aaaaagaaga tctgcataat ctgacaggtc ctatgactga agcccgcatt cgtcttcgcg
      841 aagaagtagc cgagcaaatc aaggccctga aagagattaa agtattgggc gaatattatg
      901 gtttggatct gagccgaccg gcatataccg cacaggaagc tgtacaatgg gtgtatatgg
      961 cttatctggc tgccgtaaaa gagcaggatg gtgctgccat gtcactgggt aatgtatctt
     1021 cattcctcga catctatctt gaatatgaat taagccaggg tacgataacc gaaacattcg
     1081 ctcaggaact gatcgaccaa ttcgttatca aactccgtat ggtacgccac ttgcgtatgc
     1141 agtcgtacaa cgatattttt gcgggtgatc ccacatgggt gaccgaatct atcggtggac
     1201 gtttcaatga tggacgtacc aaagttacta aaacttcttt ccgcttcttg cagacacttt
     1261 ataacttagg cccgtcaccg gaacctaaca tgactgtact atggagccct gagttacccg
     1321 aaggtttcaa agcattctgt gcacaagtat ctatcgacac ttcttccgta caatacgaaa
     1381 acgacgacct aatgcgtgaa gtccgtcaat cggatgacta cggaatcgct tgttgtgttt
     1441 catatcagga aatcggaaag cagattcagt tcttcggcgc acgtgccaac cttgccaaag
     1501 ccctgctact ggctatcaac ggaggacgtt gcgaaaatac aggtacagtt atggtaaaag
     1561 acatccctgt attgaccggt gaaacactga agttcgaaga agtaatggcc aactataaga
     1621 aagtattgat acaaattgca cgtgtataca acgaagcaat gaatatcata cattacatgc
     1681 acgataagta ttactacgaa aaggcgcaga tggctttcgt ggatactgat ccccgcatta
     1741 acctcgctta tggtgtagcc ggactttcaa tcgccatcga ttcactgtcc gccatcaaat
     1801 atgcacatgt gaaagcccgt cgcaacgaca tcggactgac ggagggattc gatatcgaag
     1861 gtgctttccc ttgtttcggt aatgacgatg accgtgtaga ccacctgggt gtagacctgg
     1921 tatatttctt tagcgaagaa ttaaagaaac tccccgtata taagaatgcc cgtcctaccc
     1981 tctcgctgct gactatcact tcgaacgtaa tgtatggcaa gaagaccggt gccactcctg
     2041 acggacgcgc caaaggcatt gcctttgctc ccggtgccaa ccccatgcac ggacgtgata
     2101 aaaatggtgc aatcgcctca ctgagttctg tagccaagtt gcgttatcgc gactcacagg
     2161 atggtatcag caatacattc tcgatcgttc ccaaatcatt gggagcaaca caagaggacc
     2221 gtatcgacaa tctggtgacc atgatggacg gatattttac aaaaggcgca caccacctga
     2281 acgtaaacgt gctgaaccgg gaaatgttgc aggatgcgat ggaacatccc gaaaagtatc
     2341 cgcaactgac tatccgtgtc tcaggatatg ccgttaattt cgtgaaattg agccgcgaac
     2401 atcagttgga agtaatcagc cgcagtttcc acgaaagaat gtaattgaat gataaacgta
     2461 cactcatacg agtcaatggg aacattcgac gggccgggtc tccggctcgt cgttttcctt
     2521 caagggtgta acttccgttg cctctattgt gccaatccgg acacaattga agccaaagga
     2581 gggacagcaa ccgaccctga ggaaatagtt cgcatggccg tcagtcagaa agctttcttc
     2641 ggtaaaaagg gaggtatcac cttttccggt ggagagccta cttttcaagc aaaatcgctt
     2701 atcccacttt ttaaaagact aaaggaagca ggaatccata tttgccttga taccaatggc
     2761 gggctatgga acaatgatgt agaggaacta ttggaattga ctgatctggt tttattagat
     2821 atcaaagaat ttaatccgga acatcatcag tctttaaccg gaagaagcaa cgagcagacc
     2881 ctgaaaaccg cagcctggct tgaaaccaat cataaaccat tctggttgcg atacgtatta
     2941 gtgcccggct acagtgactt cgaagacgat atccggcagc tgggagagca tcttggaacg
     3001 taccagatga ttcagcgtgt agaaatattg ccttaccaca ccttgggtgt tcacaaatat
     3061 gaagcaatga acaaagaata tatgctgaaa ggagtgaaag agaatactcc ggagcagata
     3121 gaaaaggctg aaaaactatt caggcaatat ttccggaccg tacaagtgaa ttgacttata
     3181 taagtgtcga aattcgtatt cgccacagat tacacgaatt tacacagata aagattcatt
     3241 ggccatcaat agattaagtt cact
//
