LOCUS       c00498_gnlPro..         5088 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DOOGDFOH_763.
ACCESSION   c00498_gnlPro..
VERSION     c00498_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 22:26:50
            Original ID  :: gnl|Prokka|DOOGDFOH_763
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5088
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5088
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[257:4896]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      258..4896
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..5088
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5088
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(3..110)
                     /locus_tag="ctg498_1"
                     /transl_table=11
                     /translation="MRTRTMKPLFLIFCLCLSPLLMGQNQNKIQEAMANY"
     CDS             complement(258..1418)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg498_2"
                     /sec_met_domain="OAD_beta (E-value: 2.4e-154, bitscore:
                     502.1, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDFVSFLGNNLVDFWGYTGFANATPGHLFMLLIGLFFIYLAVAK
                     EFEPMLLIPIGFGILIGNIPFNMEAGLKVGIYEEGSVLNILYQGVTSGWYPPLIFLGIG
                     AMTDFSALISNPKLMLIGAAAQFGIFGAYIIALLIGFEPNQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAVSAYSYMALVPVIQPPIMRALTTKHERLIRMKPPRIVSHTEKVIFP
                     IVGLLLTCFLVPSGLPLLGMLFFGNLLKESGVTRRLANTASGPLIDVITILLGLTVGAS
                     TQASQFLTWDSILIFILGAFSFIIATASGVMFVKFFNLFLKKGNKINPLIGNAGVSAVP
                     DSARISQVIGLEYDPTNYLLMHAMGPNVAGVIGSAVAAGILLGFLM"
     CDS             complement(1421..1852)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 69.7; E-value:
                     4.8e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg498_3"
                     /sec_met_domain="Biotin_lipoyl (E-value: 4.7e-23, bitscore:
                     69.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQYKYKINGNLYNVTVNDVEDNIANVEVNGTSYKVELDKPVKAA
                     PKPVTRPAAAPKTETGAPVVTKQPTASKKDGVKSPLPGVILDIKVKEGDTVKRGQTIII
                     LEAMKMENNINANKDGKVAEIKVNKGDSVLEGTDLVIIE"
     CDS             complement(1878..2792)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg498_4"
                     /sec_met_domain="OAD_gamma (E-value: 7.5e-17, bitscore:
                     50.6, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKKRIGIFIALLAMVCMGLRAQSATSLRINEVLVVNDQNYQDDY
                     GLHNAWIEIFNTSFASVNLEGCFLTNDKNNPTKYPIPKGDVLTLIKPRQHALFWADGMP
                     NRGTFHVNFTLDPNKENYIALYDSNGKTLIDEVTIPAGQLADRSYAREKDGSANWVVKG
                     EGEHSYVTPSTNNMTIDKNPKIENFKKHDSIGIGMAIIAMSVVFIGLVLLYLSFKAVGN
                     VAVRLGKKNAMKATGITDKTEAKEKNLGSHTGEETAAIAMALHEYLNDAHDVEDMILTI
                     NKVKRTYSPWSSKIYTLRQTPKR"
     CDS             complement(2813..4366)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg498_5"
                     /sec_met_domain="CT_Btheta (E-value: 1.2e-291, bitscore:
                     955.9, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKVKELIELRAQARLGGGEKAIEKQHAKGKYTARERIAQL
                     LDEGSFEELDMFVQHRCTNFGQEKKHFLGDGVVTGYGTIEGRLVYVFAQDFTVFGGSLS
                     ETMAQKICKVMDMAMKMGAPVIGINDSGGARIQEGINALSGYAEIFQRNIMASGVIPQI
                     SGIFGPCAGGAVYSPALTDFTLMTEGTSYMFLTGPAVVKTVTGEDVSQEDLGGASVHAS
                     KSGVTHFTAETGEEGLAIIRKLLSFIPQNNLEEAPLVNCTDPIDRMDDLLNEIIPDSPN
                     KPYDMYEVIGAIIDNGEFLEVQKDYAKNLIIGFARMNGQSVGVVANQPKYLAGVLDSNA
                     SRKGARFVRFCDAFNIPLVTLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKVTV
                     TLRKSYGGSHIVMSCKQLRGDMNYAWPTAEIAVMGGAGAVAVLYAKEAKDQENPAQFLA
                     DKEAEYTKLFANPYNAAKYGYIDDVIEPRNTRFRVIRALQQLQTKKLTNPAKKHGNIPL
                     "
     CDS             complement(4492..4896)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg498_6"
                     /sec_met_domain="Glyoxalase_4 (E-value: 1.3e-24, bitscore:
                     75.4, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKISHIEHLGIAVKSIEEALPYYENVLGLKCYNIETVEDQKVRTA
                     FLKVGDTKIELLEPTCPESTIAKFIENKGAGVHHVAFAIEDGVANALAEAESKEIRLID
                     KAPRKGAEGLNIAFLHPKSTLGVLTELCEH"
ORIGIN
        1 tcatagtttg ccattgcttc ctgaattttg ttttgattct ggcccatcag taggggactg
       61 aggcaaagac agaaaataag gaagagtggt ttcattgttc ttgttctcat aggacgtatt
      121 gttttattgt ccgcaagtta gtaaaaatat ccattctacg aaaggttcgt aatatctttt
      181 agatagatta acgcaaaaaa aagacgccct tctttgcgaa aagaaggacg tctaccttac
      241 taatgaaaaa atatcgttta catgaggaat ccgagcagga taccggcagc tacagccgaa
      301 ccgataaccc ccgctacgtt aggacccatg gcgtgcatca gcagatagtt ggtcggatca
      361 tattccaaac cgattacctg tgaaatacgt gccgagtcgg gtacggcgga tacacctgca
      421 ttaccaatca gcggattgat cttattgcct ttcttcaaga atagattgaa gaatttgaca
      481 aacatgacac ctgacgctgt tgcgatgatg aaagagaatg caccgaggat aaagatcaag
      541 atagaatccc aggtgaggaa ttgtgaagct tgggtagaag ctcccacagt cagtcccaac
      601 aggatagtga tcacatcgat caacggacca ctggccgtat ttgccaaacg acgggttact
      661 ccactttctt tcagaagatt accgaagaac agcataccca gcaaaggcag acccgaagga
      721 accaggaagc atgtaagcaa cagacccacg atggggaaga ttactttttc cgtatgcgat
      781 acgattctcg gtggtttcat gcggatcaga cgctcgtgtt tggtggttag cgcacgcatg
      841 ataggtggct gtatcaccgg caccagcgcc atgtaagaat aggctgacac agcgatggca
      901 cccatcaggt tgggagccag tttggacgaa aggaagatgg ccgtcggtcc gtcggcaccg
      961 ccaatgatac cgatagcacc cgcctggttg ggttcgaatc caatgagcag ggcaatgata
     1021 tacgcgccga agataccgaa ttgcgcggca gctccgatca gcatcaattt ggggttggag
     1081 atcagcgccg agaagtccgt catggctccg atacccagga aaatgagcgg cggataccag
     1141 ccggaggtta ctccttgata aaggatgttc agtaccgaac cttcttcgta aatacccact
     1201 tttaatccgg cttccatatt gaatggaata ttaccgatca gaataccgaa gccaatgggg
     1261 atcagcagca tcggttcaaa ttctttggct acggcaaggt agataaagaa gagaccgatt
     1321 agcaacatga aaagatgtcc gggtgtcgcg ttcgcaaaac cggtgtatcc ccagaagtca
     1381 acaaggttat tccctaagaa tgatacgaaa tctcccataa ttattcaatg attacgaggt
     1441 ctgtaccttc aagtacagaa tctcctttat taactttaat ttctgctact tttccgtctt
     1501 tattggcatt gatgttgttt tccatcttca tagcctcaag gatgatgatc gtctggcctc
     1561 tcttcacggt atccccttct ttcactttta tgtcgaggat aacgcccgga agcggagatt
     1621 tcacaccgtc ttttttggaa gctgtcggtt gtttggttac taccggagca cctgtttccg
     1681 ttttcggagc ggctgccgga cgggtcaccg gtttcggagc ggctttgacg ggcttgtcca
     1741 actctacttt ataagaggtt ccgttcactt caacgttggc tatgttatct tctacatcgt
     1801 tcactgtaac gttgtaaagg ttgccgttga ttttatattt atattgtttc attgcttcat
     1861 tttattaggg ttagtaatta tcttttcgga gtctgacgca gtgtgtagat cttcgaactc
     1921 cagggtgagt aggtgcgttt cactttgttg atggtcaaaa tcatgtcttc aacgtcgtga
     1981 gcgtcattca ggtattcatg caaagccatg gcgattgcgg cggtttcttc acctgtgtgg
     2041 ctacccaggt tcttttcctt cgcttccgtc ttgtcggtga taccggtggc tttcatcgcg
     2101 tttttcttac ccaatctgac agctacgtta cctaccgcct tgaatgaaag atacaagagt
     2161 accaaaccta tgaatacgac cgacatggcg atgatggcca taccgatacc gatagagtcg
     2221 tgtttcttga agttctcgat cttcgggttt ttgtcgatag tcatattgtt ggtgctcgga
     2281 gtgacatagc tgtgttctcc ttcacctttt actacccagt tggcacttcc gtctttttcg
     2341 cgagcgtatg aacggtcggc aagctgaccg gcgggtatgg ttacttcgtc aatcagagtt
     2401 ttaccgttag agtcatacaa agcaatgtaa ttctctttgt tcgggtcaag cgtaaagttc
     2461 acgtggaacg taccccggtt aggcattccg tctgcccaga acaaagcatg ttggcgcggc
     2521 ttgatcagag tcagcacgtc gcctttggga ataggatatt tggtcggatt gtttttatcg
     2581 ttcgtcagaa agcaaccttc cagatttaca gaagcgaaag aggtgttgaa tatctctatc
     2641 cacgcattgt gcagtccgta gtcatcctga tagttctggt cgttgacgac caaaacctca
     2701 ttgattctca gactggtggc actttgcgcc ctgagcccca tgcaaaccat tgccagcaga
     2761 gcgataaata tcccgattct tttcttattc ataacaatcg atgtttaatt tgttacaatg
     2821 gaatattacc gtgcttttta gcagggttgg ttaatttttt ggtctgcagc tgttgcaggg
     2881 cgcggatcac gcggaaacga gtgtttctcg gttcgataac atcgtcgatg taaccgtatt
     2941 tggctgcatt gtacgggttg gcgaacagtt tagtgtactc ggcttccttg tctgccagga
     3001 attgagcagg gttttcctga tctttggctt ctttggcata caataccgct actgcaccgg
     3061 caccgcccat tacggcgatt tcggctgtcg gccatgcata gttcatgtcg ccgcggagtt
     3121 gcttgcaact cattacgatg tgcgaaccac cgtaagattt acgcagggta acagttacct
     3181 tgggcacagt ggcttcacca taagcgtaca gcaacttggc tccgtgaagg attacaccat
     3241 tatattcctg acccgtaccc ggaaggaatc ccggaacgtc taccaatgtc accagcggaa
     3301 tgttgaatgc gtcgcagaag cgaacgaagc gtgcaccttt gcgtgaagca ttgctgtcga
     3361 gtactccggc gaggtattta ggctgattgg caaccacacc taccgattgt ccgttcatac
     3421 gggcaaaacc gataataaga tttttcgcat agtctttctg tacttccagg aattctccgt
     3481 tatcgatgat ggcgccgatc acttcgtaca tatcgtacgg tttgttcggg ctgtcgggga
     3541 tgatctcgtt cagcaggtcg tccatacggt cgatggggtc ggtacagttc accaatggag
     3601 cttcttccag gttgttttgc ggaataaagc tgagaagctt gcgaataatc gccagacctt
     3661 cttcaccggt ttcggcagtg aagtgagtta caccggactt gctggcatgt acgcttgcac
     3721 cacccagatc ttcctgagac acgtcttcac ccgttactgt tttcacaaca gccggtccgg
     3781 tgaggaacat gtaagatgta ccttccgtca tcagcgtgaa gtccgtcagg gcgggagagt
     3841 aaaccgcacc gccggcacac ggaccgaaaa tacctgaaat ctgtgggatg acacccgaag
     3901 ccatgatgtt gcgctggaag atttcagcat aaccggacag ggcgttgatg ccttcctgaa
     3961 tacgtgcgcc acccgagtcg ttgataccga taacaggggc acccatcttc atggccatat
     4021 ccattacttt acagatcttt tgtgccatgg tttccgacag tgaaccaccg aatactgtga
     4081 aatcttgtgc gaaaacatat actaatctac cttctatcgt tccataaccg gttaccacac
     4141 cgtcgccgag gaaatgtttt ttctcttgtc cgaaattggt acatctgtgt tgaacaaaca
     4201 tatccagttc ttcgaaacta ccttcgtcaa gcaactgtgc aatacgctcg cgggctgtat
     4261 atttgccttt ggcgtgttgt ttttcaatag ccttttcgcc accacctaaa cgagcctgag
     4321 cacgaagttc aataagctct tttacttttt caagctggtt actcatgagt ttttcttaat
     4381 ttaaagggtt ataatattta caaccggacg attgacaacc cggatgggga atttaatgca
     4441 aagaaaaatt ccgtttgtcc gtggcgcgga tcgtccgaat cgtacatctt tttagtgttc
     4501 gcagagttct gtcagtacgc ccagagttga tttcgggtga aggaaagcaa tgttcaggcc
     4561 ttcggcacct ttgcgcggag ctttgtcgat caggcggatc tctttgcttt cggcttcggc
     4621 cagggcattg gctacgccgt cttcaatggc gaatgctacg tgatgaacac ctgcaccttt
     4681 gttttcaatg aatttcgcga tggtactttc cggacatgtg ggttccagta attcgatttt
     4741 ggtatcacct actttcagga aggctgttct tactttttga tcttctacag tttcgatgtt
     4801 gtagcatttc aaacctaaaa cgttttcata atacggaagg gcttcttcga tactttttac
     4861 ggcaatgccc aaatgttcaa tgtgtgaaat cttcataact atatttttaa attttggtat
     4921 taatcgtctt ttcggaatgg tataaaacag cacaaagaaa atcaattctt gtttaataaa
     4981 gaaatttttc tgcaattaat tatggtgaaa ataggtttaa aacctaaggt gcatgggttt
     5041 aaaaggcagg catcgttggt ttaaaaccta aggggtgttg gtttaaaa
//
