GCA_001633265.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaMINOPLBK_10 (original name was: gnl|Prokka|MINOPLBK_10)

Region	Type	From	To	Most similar known cluster		Similarity
Region 9.1	cyclic-lactone-autoinducer	220,966	241,672

gnlProkkaMINOPLBK_21 (original name was: gnl|Prokka|MINOPLBK_21)

Region	Type	From	To	Most similar known cluster		Similarity
Region 19.1	RiPP-like	3,077	14,336	salivaricin CRL1328 α peptide/salivaricin CRL1328 β peptide	RiPP	16%

gnlProkkaMINOPLBK_27 (original name was: gnl|Prokka|MINOPLBK_27)

Region	Type	From	To	Most similar known cluster		Similarity
Region 24.1	NRPS,NRPS-like	161,448	220,043

gnlProkkaMINOPLBK_35 (original name was: gnl|Prokka|MINOPLBK_35)

Region	Type	From	To	Most similar known cluster		Similarity
Region 31.1	T3PKS	625,685	666,854

gnlProkkaMINOPLBK_38 (original name was: gnl|Prokka|MINOPLBK_38)

Region	Type	From	To	Most similar known cluster		Similarity
Region 33.1	terpene	27,675	48,556

Region	Type	From	To	Most similar known cluster	Similarity
Region 9.1	cyclic-lactone-autoinducer	220,966	241,672
Region 19.1	RiPP-like	3,077	14,336	salivaricin CRL1328 α peptide/salivaricin CRL1328 β peptide	RiPP	16%
Region 24.1	NRPS,NRPS-like	161,448	220,043
Region 31.1	T3PKS	625,685	666,854
Region 33.1	terpene	27,675	48,556

Compact view

gnlProkkaMINOPLBK_10 - Region 1 - cyclic-lactone-autoinducer

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Location: 220,966 - 241,672 nt. (total: 20,707 nt) Show pHMM detection rules used

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

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gnlProkkaMINOPLBK_21 - Region 1 - RiPP-like

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Location: 3,077 - 14,336 nt. (total: 11,260 nt) Show pHMM detection rules used

Region on contig edge.

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

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gnlProkkaMINOPLBK_27 - Region 1 - NRPS,NRPS-like

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Location: 161,448 - 220,043 nt. (total: 58,596 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

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Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 161447 - 220043:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Ala) + (D-Ser - Gly) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 161447 - 216229:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Ala) + (D-Ser - Gly) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 177625 - 220043:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Ala) + (D-Ser - Gly) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg24_176

AMP-binding (8..362): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SVTQAAVAGK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

ctg24_177

Search NORINE for peptide: strict or relaxed

AMP-binding (637..1009): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DSHGYGCVLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

AMP-binding (1604..1970): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLIVAAVIK
Nearest Stachelhaus code(s):: Ala DVWVVAAVIK (68% 8Å match); S-bohPhe or Phe DALIVAAVCK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

ctg24_178

Search NORINE for peptide: strict or relaxed

AMP-binding (45..431): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1451..1829): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DITQMGIVWK
Nearest Stachelhaus code(s):: Gly DILQMGMVWK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

ctg24_181

AMP-binding (13..374): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VVYNTAVCAK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

gnlProkkaMINOPLBK_35 - Region 1 - T3PKS

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Location: 625,685 - 666,854 nt. (total: 41,170 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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gnlProkkaMINOPLBK_38 - Region 1 - terpene

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Location: 27,675 - 48,556 nt. (total: 20,882 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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core biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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