LOCUS       c00201_gnlPro..         3164 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NPNBONHK_230.
ACCESSION   c00201_gnlPro..
VERSION     c00201_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-30 04:57:01
            Original ID  :: gnl|Prokka|NPNBONHK_230
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3164
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3164
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[231:3163]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      232..3163
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3164
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3164
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             232..1437
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg201_1"
                     /sec_met_domain="PFL_acetate2 (E-value: 5.1e-151, bitscore:
                     491.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQLDETKVPNYWEGFNGGDWQEEINVRDFIEHNLNQYDGDESFL
                     AGPTEATTVLNNQVLNLKKQERANGGVLDADNNIPSTITSHGPGYLNKDLEKIVGVQTD
                     KPFKRAFMPFGGIRMAEDALKSYGFETDANEHKIFTEYRKTHNQGVFDAYTPEMRKARH
                     YKIVTGLPDAYGRGRIVSDFPRIAIYGIDRLMAEKFNDYNLTGDGEMTDDVIKLREEIN
                     EQYRALNDMKKMAKEYGYDISRPAANAQEAVQWIYFGYLAAVKTQNGAAMSVGRIDTVI
                     DAYIQRDMRLGKLNEEQAQELIDHLVMKLRMVRFIRTEEYNSLFSGDPIWATLSLAGMG
                     NDGRHHVTKTAFRFLKTLDNMGAAPEPNITLLWSERLPKGSNVMLLKCPSKVQPFSTKT
                     MI"
     CDS             1437..2492
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg201_2"
                     /sec_met_domain="Gly_radical (E-value: 3.3e-43, bitscore:
                     134.4, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.3e-79, bitscore:
                     255.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRNEWGTDYYGIACCVSAQPIADGVQYFGARANLAKTVLYAINGG
                     KDEIQEAQVGPEYEPITSDYIDYDEFMKKFDKMMDWLADVYVNALNVIHYMHDKYYYEA
                     AQLALKDTRLNRTFATGISGLSHAVDSISAIKYGHVKAIRDENGVAIDFVADNDDYPRY
                     GNNDDRADNIAKWLVKTFYNKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTTPNGRQK
                     GEPFSPGANPAYGAEKNGALASLMSTAKIPYHYATDGISNTFGVTPNTLGHDDETRKDT
                     LVHMVDGYMENSGMHLNINVFNKETLIDAQKHPEEYPTLTVRVSGYCVYFADLTKEQQD
                     DVIARTFFDEM"
     CDS             2630..3163
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 68.5;
                     E-value: 2.4e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg201_3"
                     /sec_met_domain="Radical_SAM (E-value: 4.4e-24, bitscore:
                     73.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLIDQSQSPLNILEQVGQDHEGPIKGYVHSVESFGSVDGPGIRF
                     VVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILADAQRYKAFWGEQGGITCSGGESLV
                     QIDFILELFTKAKELGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAE
                     HKKLTGFPNENILDM"
ORIGIN
        1 catctgtatt aatacagatg gagaattttt tatttattat atgacagtac ggttgtgcac
       61 aacttatcaa gctaatgatt aagctttttt tatgaaacag gaaagtacac cgaaaaaaac
      121 agcaaaacgc ctgttatatc agcgataaaa cgcttgcata gatttgcctt cgtgttatac
      181 tgagtttgta agaactgtca catctgtaat tcactttagg aggcaatacc tatgaaacag
      241 cttgacgaaa ccaaagttcc taattattgg gaaggcttta acggcggcga ctggcaagaa
      301 gaaattaacg tgcgcgattt tatcgagcat aacctgaacc aatatgatgg ggacgagagt
      361 ttccttgctg gccccactga agcaacgact gttttaaata atcaggtttt gaatctgaaa
      421 aagcaagaac gtgccaatgg cggtgtactg gatgcagata acaatattcc atcgaccatt
      481 acttctcatg gccccggtta tctgaataag gacctcgaaa agattgtcgg tgtccaaacc
      541 gataagccgt ttaagcgagc attcatgcca tttggcggta tccggatggc tgaggatgct
      601 ttgaaatcct atggcttcga aaccgacgct aatgagcaca agatctttac cgaatatcgt
      661 aagacgcata accaaggtgt ttttgatgct tatacgccgg aaatgcgcaa agcccgccac
      721 tataagattg tgaccggctt accggatgct tatggtcgtg gtcggatcgt ttcggacttc
      781 cctcgaattg ctatttatgg gattgaccgg ctgatggctg agaagttcaa cgattacaac
      841 ctgaccggcg atggcgagat gacagatgat gttatcaaac tgcgcgaaga aattaatgaa
      901 cagtatcgtg ccttgaatga catgaagaag atggccaaag aatacggcta tgacatttcc
      961 cgcccagctg ctaatgctca agaagctgtt cagtggatct acttcggtta cctggcagcc
     1021 gttaagaccc aaaatggtgc ggcaatgtct gtcggccgta tcgatacagt gattgatgcc
     1081 tatattcaac gggatatgcg tttgggcaag ctgaatgaag agcaggcaca agaactcatc
     1141 gatcatttgg ttatgaagtt acggatggta cgtttcattc ggactgaaga atacaactca
     1201 ctgttctccg gtgatccgat ctgggcaaca ctgtccttgg cagggatggg taatgatggc
     1261 cgtcatcatg ttaccaagac cgcgttccgc ttcctgaaga ccttggacaa catgggcgcg
     1321 gcaccggaac caaacatcac cttgttgtgg agcgaacgtc ttcctaaggg ttcaaacgtt
     1381 atgctactga agtgtccatc caaagttcaa ccattcagta cgaaaacgat gatctgatgc
     1441 gcaacgaatg gggaaccgac tattacggca ttgcttgctg tgtttccgca cagccaattg
     1501 cagatggtgt tcagtacttc ggggcgcgag caaacctagc caagaccgtg ctgtatgcga
     1561 tcaacggtgg taaggacgaa attcaggaag cacaggtggg acctgaatac gagccgatca
     1621 ccagcgacta cattgactat gacgagttca tgaagaagtt cgacaagatg atggactggt
     1681 tagcagatgt ctatgtcaat gccctgaatg ttattcacta catgcacgac aagtactact
     1741 atgaagctgc gcagttggca ttgaaggata cacgtctgaa tcggacgttt gctaccggga
     1801 tttccggttt gtcacatgcg gttgattcca tcagtgccat caagtatggc cacgtgaagg
     1861 caattcggga tgaaaatggc gttgcaatcg actttgttgc tgacaatgat gactatccgc
     1921 gttatggtaa caatgatgac cgggcagaca acattgctaa gtggttggtt aagaccttct
     1981 acaacaagat gaatacacat catctgtatc gcggtgctaa gctcagtacc agtgttctga
     2041 ccattacatc aaatgtggtt tatggtaaga ataccgggac aacaccaaac ggccgtcaga
     2101 agggcgaacc attctcacct ggagccaacc cagcgtatgg cgctgaaaag aacggtgctt
     2161 tagcttcctt gatgtcaacc gccaagatcc catatcacta tgcaacagac gggatcagca
     2221 atacctttgg ggtaacaccg aacaccttag gccatgacga tgaaactcgt aaggacacct
     2281 tggttcacat ggttgacggc tacatggaaa atagcggcat gcaccttaac atcaacgtct
     2341 tcaataaaga aacgttgatt gatgcccaga agcacccaga agaatatcca actttgactg
     2401 ttcgagtttc cggttactgc gtctactttg cagacctgac caaagaacag caagacgatg
     2461 ttatcgctcg gaccttcttc gacgaaatgt aatcaagtca atctattcgc aaaacaggag
     2521 atcattcctg ttttgtcaaa aaacggcaac gatgctgttg ccgttttttg acaaactgaa
     2581 atgaaaatcg ttgaaaatta ggtatttatt tgcactggag gcggcataga tgaaactcat
     2641 cgatcaatca caaagcccgt taaatatttt ggaacaagtg ggacaggatc atgaaggccc
     2701 aataaaggga tacgttcatt cagtcgaaag ctttggttcg gttgatggtc ccgggattcg
     2761 gtttgtcgtc tttatgcaag gatgcaggat gcggtgccag tactgtcaca accctgacac
     2821 ttggaatatc ggtgtgggcg aggaaatgac cgctgatcag atcctggcag atgcacagcg
     2881 atataaagcc ttctggggtg aacaaggggg gattacttgt agcggtggcg aaagtctcgt
     2941 ccaaattgat tttatcctgg aactttttac caaagctaag gaactgggta tctcaacttg
     3001 tttggatact tcgggtggtc cattcacccg cgatcagccg tggtttggtc aatttgagaa
     3061 actgatggcc gtcacggata tttcgttggt tgatatcaag cacattgatt ccgcggaaca
     3121 caaaaagctg accggtttcc caaacgaaaa cattttggat atgg
//
