LOCUS       c00271_gnlPro..         6049 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LMPAEMNE_292.
ACCESSION   c00271_gnlPro..
VERSION     c00271_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-30 04:41:09
            Original ID  :: gnl|Prokka|LMPAEMNE_292
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6049
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6049
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:2566]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..2566
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6049
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6049
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..1577
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg271_1"
                     /sec_met_domain="Gly_radical (E-value: 1.1e-42, bitscore:
                     133.4, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2.9e-168, bitscore:
                     549.3, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="DMKKMAKEYGYDISRPAANAQEAVQWIYFGYLAAVKTQNGAAMSV
                     GRIDTVIDAYIQRDMRLGKLNEEQAQELIDHLVMKLRMVRFIRTEEYNSLFSGDPIWAT
                     LSLAGMGNDGRHHVTKTAFRFLKTLDNMGAAPEPNITLLWSERLPEGFKRYATEVSIQS
                     STIQYENDDLMRNEWGTDYYGIACCVSAQPIADGVQYFGARANLAKTVLYAINGGKDEI
                     QEAQVGPEYEPITSDYIDYDEFMKKFDKMMDWLADVYVNALNVIHYMHDKYYYEAAQLA
                     LKDTRLNRTFATGISGLSHAVDSISAIKYGHVKAIRDENGVAIDFVADNDDYPRYGNND
                     DRADNIAKWLVKTFYNKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPF
                     SPGANPAYGAEKNGALASLMSTAKIPYHYATDGISNTFGVTPNTLGHDDETRKDTLVHM
                     VDGYMENSGMHLNINVFNKETLIDAQKHPEEYPTLTVRVSGYCVYFADLTKEQQDDVIA
                     RTFFDEM"
     CDS             1715..2566
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 66.6;
                     E-value: 9e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg271_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.4e-27, bitscore:
                     84.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLIDQSQSPLNILEQVGQDHEGPIKGYVHSVESFGSVDGPGIRF
                     VVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILADAQRYKAFWGEQGGITCSGGESLV
                     QIDFILELFTKAKELGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAE
                     HKKLTGFPNENILDMVQYMSDHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKRVVQ
                     KVEILPYHTLGVKKYHELGITYPLEGIEPPSADRVKNAENLLHVEDYTGWQSWRPQPAN
                     KQ"
     CDS             2688..3656
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     55.5; E-value: 1.4e-17)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg271_3"
                     /sec_met_domain="LysR_substrate (E-value: 8.8e-28,
                     bitscore: 85.5, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 1.1e-18, bitscore: 55.5,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKQERIFSSKTLTYFLQLAETMNYTQSAQLLGITQPALTQQIK
                     KLERTVGAPLFYSVGKKLHLSDAGRTMLKATHEIYEILNQTTDEIQQSTSATVGKINIG
                     LLSSIEDSAFNAFIAHYYDLNPEVEITLRMMTRHEIWEALENNQIDLAIMNLPDETIKN
                     WKPYASKKILDEQLLFLHHDNSLANRKRLHFKDTLAEPWVTYPDDYFLGQLLHEAYKNQ
                     MVDFPHVVAHFTTPYQLTQFVNHTGVNTALPTSFVAAHADQIKAHAVAFEPAIRFETSF
                     VYRKEKADIPRIANFFKTFDAHLSEKDYLSRVSENSGKIER"
     CDS             3686..4618
                     /locus_tag="ctg271_4"
                     /transl_table=11
                     /translation="MTTLIFGHQNPDTDAITSAMSWAEFQKQAGNTDVEAVALGEPNDE
                     TKFVLDHFKVAAPRVIKTAANETGHVMLVDHNEFQQSVADIEDVQIDSVVDHHRIANFH
                     TASPLYYRAEPVGSTNTVIFELFQEHGYEISPTLAGLMASGLISDTLLLKSPTATDKDK
                     KILPEMAKIAGIDLQSYGLAMLKAGTNLAAKSDMDIIEGDAKSFTMGGKSIRIGQVNTV
                     DLDDIYARQDELVAAMTKEAQAKGYDDFLLIATDILNSNSTGFAVGADRDKVAAAFNEK
                     FDANDRLALPGVVSRKKQVVPPMQGAFEG"
     CDS             5504..6049
                     /locus_tag="ctg271_5"
                     /transl_table=11
                     /translation="LSTQNYPKLIQAMLPSLPDYIQDYYHSQKAIPIADSTLYQYLHFY
                     QEFLHWLISSRVTDAVSPQNVPLKTLERLSLRDAQAFVAYLLERPSKTHPNKRMTRRSV
                     ALRLVGIKALYRFLTEESEPNADGEPYFYRNVWNKVKLKTHAETTTYRNHKLQEMLFVD
                     GEDARFLQWLDRQYAQQLP"
ORIGIN
        1 atgacatgaa gaagatggcc aaagaatacg gctatgacat ttcccgccca gctgctaatg
       61 ctcaagaagc tgttcagtgg atctacttcg gttacctggc agccgttaag acccaaaatg
      121 gtgcggcaat gtctgtcggc cgtatcgata cagtgattga tgcctatatt caacgggata
      181 tgcgtttggg caagctgaat gaagagcagg cacaagaact catcgatcat ttggttatga
      241 agttacggat ggtacgtttc attcggactg aagaatacaa ctcactgttc tccggtgatc
      301 cgatctgggc aacactgtcc ttggcaggga tgggtaatga tggccgtcat catgttacca
      361 agaccgcgtt ccgcttcctg aagaccttgg acaacatggg cgcggcaccg gaaccaaaca
      421 tcaccttgtt gtggagcgaa cgtcttcctg aagggttcaa acgttatgct actgaagtgt
      481 ccatccaaag ttcaaccatt cagtacgaaa acgatgatct gatgcgcaac gaatggggaa
      541 ccgactatta cggcattgct tgctgtgttt ccgcacagcc aattgcagat ggtgttcagt
      601 acttcggggc gcgagcaaac ctagccaaga ccgtgctgta tgcgatcaac ggtggtaagg
      661 acgaaattca ggaagcacag gtgggacctg aatacgagcc gatcaccagc gactacattg
      721 actatgacga gttcatgaag aagttcgaca agatgatgga ctggttagca gatgtctatg
      781 tcaatgccct gaatgttatt cactacatgc acgacaagta ctactatgaa gctgcgcagt
      841 tggcattgaa ggatacacgt ctgaatcgga cgtttgctac cgggatttcc ggtttgtcac
      901 atgcggttga ttccatcagt gccatcaagt atggccacgt gaaggcaatt cgggatgaaa
      961 atggcgttgc aatcgacttt gttgctgaca atgatgacta tccgcgttat ggtaacaatg
     1021 atgaccgggc agacaacatt gctaagtggt tggttaagac cttctacaac aagatgaata
     1081 cacatcatct gtatcgcggt gctaagctca gtaccagtgt tctgaccatt acatcaaatg
     1141 tggtttatgg taagaatacc gggacaacac caaacggccg tcagaagggc gaaccattct
     1201 cacctggagc caacccagcg tatggcgctg aaaagaacgg tgctttagct tccttgatgt
     1261 caaccgccaa gatcccatat cactatgcaa cagacgggat cagcaatacc tttggggtaa
     1321 caccgaacac cttaggccat gacgatgaaa ctcgtaagga caccttggtt cacatggttg
     1381 acggctacat ggaaaatagc ggcatgcacc ttaacatcaa cgtcttcaat aaagaaacgt
     1441 tgattgatgc ccagaagcac ccagaagaat atccaacttt gactgttcga gtttccggtt
     1501 actgcgtcta ctttgcagac ctgaccaaag aacagcaaga cgatgttatc gctcggacct
     1561 tcttcgacga aatgtaatca agtcaatcta ttcgcaaaac aggagatcat tcctgttttg
     1621 tcaaaaaacg gcaacgatgc tgttgccgtt ttttgacaaa ctgaaatgaa aatcgttgaa
     1681 aattaggtat ttatttgcac tggaggcggc atagatgaaa ctcatcgatc aatcacaaag
     1741 cccgttaaat attttggaac aagtgggaca ggatcatgaa ggcccaataa agggatacgt
     1801 tcattcagtc gaaagctttg gttcggttga tggtcccggg attcggtttg tcgtctttat
     1861 gcaaggatgc aggatgcggt gccagtactg tcacaaccct gacacttgga atatcggtgt
     1921 gggcgaggaa atgaccgctg atcagatcct ggcagatgca cagcgatata aagccttctg
     1981 gggtgaacaa ggggggatta cttgtagcgg tggcgaaagt ctcgtccaaa ttgattttat
     2041 cctggaactt tttaccaaag ctaaggaact gggtatctca acttgtttgg atacttcggg
     2101 tggtccattc acccgcgatc agccgtggtt tggtcaattt gagaaactga tggccgtcac
     2161 ggatatttcg ttggttgata tcaagcacat tgattccgcg gaacacaaaa agctgaccgg
     2221 tttcccaaac gaaaacattt tggatatggt tcagtatatg tcggatcatg gcgatgatat
     2281 gtggattcgc cacgtacttg tgccagaacg aaccgacttt gatccttatc tcaaacgctt
     2341 gggagattat attgccactt tggataaacg cgtggtccag aaagtggaaa ttcttccgta
     2401 ccatacgtta ggggttaaga agtaccatga actaggtatt acgtatccgc tggaaggtat
     2461 cgagccgcct tcagctgatc gggtcaagaa tgcagagaac ttgctacacg ttgaggacta
     2521 tactggatgg caaagttggc gaccacagcc agctaacaag cagtgattta gttgaaactt
     2581 aacgtaacga ttaaaaacgc agcaaatccg ttgcgttttt tttaaatgcc acttatgatg
     2641 atggttgagt aacgttatca aattataaac gaaagggaag ccgccctatg aagacgaaac
     2701 aagagcgcat tttttcctcc aaaactctca cgtatttttt gcaattagca gaaaccatga
     2761 actacaccca gtcagcccag ttgttgggga ttacccagcc ggcactgacg cagcaaatca
     2821 agaaattgga acgaacagtg ggggcaccat tgttttattc cgttggtaag aagttgcatt
     2881 tatccgatgc cggtcgcacg atgttgaagg cgacacacga gatttatgaa attttgaacc
     2941 agacgactga cgagattcag caaagtacca gtgccacagt cggtaagatt aatattggct
     3001 tgttaagttc aattgaagac agtgcgttta atgcttttat cgcgcactac tatgatctta
     3061 atcctgaagt agaaatcacg ttgcgaatga tgacacgtca tgagatctgg gaagcattgg
     3121 agaacaacca gattgatttg gccatcatga atctgcctga tgagacgatt aagaactgga
     3181 aaccgtatgc cagcaagaag attcttgatg aacagttatt gttcctgcac catgataatt
     3241 ccctggctaa tcgcaaacgc ctgcatttca aagatacgct ggctgagccg tgggtaactt
     3301 atccagacga ctatttcctg ggtcagttgt tgcatgaggc atataagaat cagatggttg
     3361 attttccgca tgttgtggcg cattttacca cgccatatca gttaactcag tttgtcaacc
     3421 ataccggtgt taacaccgcg ttaccaacca gttttgttgc cgctcatgct gatcaaatca
     3481 aggcgcatgc cgttgccttt gaaccagcga ttcgctttga gacctcgttt gtgtatcgca
     3541 aggaaaaagc tgatattccg cggattgcca acttctttaa gacgtttgac gcacatttat
     3601 ctgagaaaga ttatctatcg cgggtcagcg aaaacagtgg taaaattgaa aggtaaacta
     3661 ttcagctgag gagcgatcag ataacatgac gactttgatt tttggacatc aaaaccctga
     3721 tacagacgcg attacaagcg ccatgagttg ggctgaattt cagaagcaag caggtaatac
     3781 cgatgttgaa gcagtagcac tcggtgaacc aaacgatgaa accaagtttg tattggatca
     3841 ttttaaggtt gctgcgccgc gggtcattaa gacagccgca aatgaaaccg ggcacgtaat
     3901 gttggttgat cataacgaat tccagcaaag cgttgctgat atcgaagacg tgcaaatcga
     3961 ttcagtggtt gatcaccacc gcattgccaa cttccatacg gcgtcaccac tatattaccg
     4021 ggcagaacca gtgggcagta ccaacacggt gattttcgag ttgttccagg aacacggcta
     4081 tgaaatttca ccaacactgg caggcttaat ggcttctggc ttgatttcgg ataccctgct
     4141 tctgaagtca ccgactgcaa ccgacaagga caaaaagatc ttgccggaaa tggctaaaat
     4201 cgctggcatt gacctgcaat catacggttt ggctatgttg aaggcgggca ccaatctagc
     4261 tgccaagtcg gatatggata tcattgaagg cgatgcgaag agctttacga tgggcggtaa
     4321 gtctatccgt attggacaag tgaatacagt tgatcttgat gatatttatg cgcgtcagga
     4381 tgaattggtg gccgctatga ccaaagaagc acaggcgaag ggctatgatg acttcctgtt
     4441 aattgcaacc gatattctga acagcaattc aacgggcttt gctgttggcg cagatcgtga
     4501 caaagtggcc gcagcattca acgaaaagtt tgatgccaat gaccggttgg cactgcctgg
     4561 cgtggtttca cgtaagaagc aggtcgtacc gccaatgcaa ggtgcgtttg aaggttaatt
     4621 ttgaacttaa taattggttt tataaatcgc agccgtttgt ggttgcgatt tttttgtgct
     4681 caaaaacttg gaagaacaaa aaagtcgaat ttttagatct gaaggtgcag aaatgtgatt
     4741 attgcgaaat ttttcagtaa gaatagttca aatttaaaac gaggtgcaaa aaacgcagcc
     4801 ttataactac ttcgtataat atatattatg taaactagaa tggagatttt gagtcgttga
     4861 ctttgacgtg acttatttgg tgttcaaatg gcaaaacgat tgatgcaagt cccctcacct
     4921 gacagacagc tcaaaaactt ttgagagaag caaatcaatt gtacggatgt gagcggtgcg
     4981 ctttatttgt ttttttctgg tttgaaaatg accagtcgtt gctaaatata tgtaaattca
     5041 gcattaatca aagcagattt gtagaaagta tcattttgag tcataaacag cattcaagtc
     5101 gcaagtgttg ctgtcagctg aattctggat caaaatacga aaattgattt gattgcagcc
     5161 aatgaatcgt tggttaattg gttaaactgt tgctaaagcg gcgccgtgga atcaggacat
     5221 cagcacagat gtcctgatcg tgcagtgaat tcaaatttga aatgcaacta agtttagtgt
     5281 caaatgacag atcaacaaat ttggtgattt agctggcttg atgattagaa gcccatttac
     5341 ctttggataa tactaaaact ttattcagtt ctacactggt gcttttttcg gcgcatatcg
     5401 ttgcgaatta ccgatatttt cgtcattatc cctcgccacg cactggtttt ttcgcgtata
     5461 ctgaaattat ccaaaagaaa acatatgttc gaggtgactt tatttgtcga cacagaacta
     5521 ccctaaatta atacaagcca tgctaccttc cctgccggac tatattcagg attactatca
     5581 cagccaaaaa gcaattccca tcgcggattc taccctttac cagtacctgc acttttacca
     5641 ggaatttctg cattggctta tttccagtcg ggtcacggat gccgtttcgc cacagaatgt
     5701 gcctttgaag accttagagc ggctttcctt gcgtgatgcg caggcgtttg tggcgtattt
     5761 acttgagcgg ccgtcgaaaa cccatccgaa taaacgcatg acgcggcgca gtgttgcgtt
     5821 acgactggtc gggatcaagg ccctttatcg gttcttaacc gaagaaagcg aaccaaatgc
     5881 agacggtgag ccttattttt accgcaatgt ttggaataag gtgaaactta agacccatgc
     5941 ggagacgacg acttaccgta atcataaact gcaggaaatg ttgtttgtag atggcgaaga
     6001 tgcgcgcttt ttacagtggc tggatcggca atatgctcag cagctgcca
//
