LOCUS       c00138_gnlPro..         7505 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EHLMDOOL_151.
ACCESSION   c00138_gnlPro..
VERSION     c00138_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-29 04:30:36
            Original ID  :: gnl|Prokka|EHLMDOOL_151
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7505
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..774
                     /locus_tag="ctg138_1"
                     /transl_table=11
                     /translation="LNNHEVLIYASNHYEPATLRIGKQTYHVAKQVTSFPNAPKTFIPN
                     LFVALPSDVQLLTALHALYPKRSMALLKADVTTNQDVTLSGSHANQVKLYHAVVKRGIA
                     LPESVQSRAANYDDTASMMSSFLFLGVVFGVTFILATLLILYYKQLAEGYADARRYTIL
                     QRVGLSLREVRATINSQLLTLFYLPLIVAAIHVGFALPFVQRIMMLFGLPDWQFFLTVS
                     LITLAIFAVIYVLMYRLTGNVYYRIVSRRQPSSRG"
     protocluster    1..7505
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[4941:7505]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      4942..7505
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7505
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7505
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(950..2062)
                     /locus_tag="ctg138_2"
                     /transl_table=11
                     /translation="LSTQNYPKLIQAMLPSLPDYIQDYYHSQKAIPIADSTLYQYLHFY
                     QEFLHWLISSRVTDAISPQNVPLETLERLSLRDAQAFVAYLLERPSKTHPNKRMTRRSV
                     ALRLVGIKALYRFLTEESEPNADGEPYFYRNVWNKVKLKTHAETTTYRNHKLQEMLFVD
                     GEDARFLQWLDQQYAQQLPSKPRAYFEATKERNLAIIALLFGSGVRVSELVNMNLEDLN
                     MDRHTVQVVRKGNFQDRVNFADWIDPYLTAYLKSRTAMIGQQKPTSPLFVTQIGQMVNR
                     IRQNTIEAFFKRYTTAYGRPSTPHKARHTLGTNIYTVTKDVQQVADQLGQTTTSATDLY
                     INLSDKSGKAALREVSETAAKALDQHPQQP"
     CDS             complement(2890..3822)
                     /locus_tag="ctg138_3"
                     /transl_table=11
                     /translation="MTTLIFGHQNPDTDAITSAMSWAEFQKQAGNTDVEAVALGEPNDE
                     TKFVLDHFKVAAPRVIKTAANETGHVMLVDHNEFQQSVADIEDVQIDSVVDHHRIANFH
                     TASPLYYRAEPVGSTNTVIFELFQEHGYEISPTLAGLMASGLISDTLLLKSPTATDKDK
                     KILPEMAKIAGIDLQSYGLAMLKAGTNLAAKSDMDIIEGDAKSFTMGGKSIRIGQVNTV
                     DLDDIYARQDELVAAMTKEAQAKGYDDFLLIATDILNSNSTGFAVGADRDKVAAAFNEK
                     FDANDRLALPGVVSRKKQVVPPMQGAFEG"
     CDS             complement(3852..4820)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     55.5; E-value: 1.4e-17)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg138_4"
                     /sec_met_domain="LysR_substrate (E-value: 1.1e-27,
                     bitscore: 85.5, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 1.3e-18, bitscore: 55.5,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKQERIFSSKTLTYFLQLAETMNYTQSAQLLGITQPALTQQIK
                     KLERTVGAPLFYSVGKKLHLSDAGRTMLKATHEIYEILNQTTDEIQQSTSATVGKINIG
                     LLSSIEDSAFNAFIAHYYDLNPEVEITLRMMTRHEIWEALENNQIDLAIMNLPDETIKN
                     WKPYASKKILDEQLLFLHHDNSLANRKRLHFKDTLAEPWVTYPDDYFLGQLLHEAYKNQ
                     MVDFPHVVAHFTTPYQLTQFVNHTGVNTALPTSFVAAHADQIKAHAVAFEPAIRFETSF
                     VYRKEKADIPRIANFFKTFDAHLSEKDYLSRVSENSGKIER"
     CDS             complement(4942..5793)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 66.6;
                     E-value: 9e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg138_5"
                     /sec_met_domain="Radical_SAM (E-value: 2.8e-27, bitscore:
                     84.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLIDQSQSPLNILEQVGQDHEGPIKGYVHSVESFGSVDGPGIRF
                     VVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILADAQRYKAFWGEQGGITCSGGESLV
                     QIDFILELFTKAKELGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAE
                     HKKLTGFPNENILDMVQYMSDHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKRVVQ
                     KVEILPYHTLGVKKYHELGITYPLEGIEPPSADRVKNAENLLHVEDYTGWQSWRPQPAN
                     KQ"
     CDS             complement(5931..7505)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg138_6"
                     /sec_met_domain="Gly_radical (E-value: 1.4e-42, bitscore:
                     133.4, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 3.5e-168, bitscore:
                     549.3, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="DMKKMAKEYGYDISRPAANAQEAVQWIYFGYLAAVKTQNGAAMSV
                     GRIDTVIDAYIQRDMRLGKLNEEQAQELIDHLVMKLRMVRFIRTEEYNSLFSGDPIWAT
                     LSLAGMGNDGRHHVTKTAFRFLKTLDNMGAAPEPNITLLWSERLPEGFKRYATEVSIQS
                     STIQYENDDLMRNEWGTDYYGIACCVSAQPIADGVQYFGARANLAKTVLYAINGGKDEI
                     QEAQVGPEYEPITSDYIDYDEFMKKFDKMMDWLADVYVNALNVIHYMHDKYYYEAAQLA
                     LKDTRLNRTFATGISGLSHAVDSISAIKYGHVKAIRDENGVAIDFVADNDDYPRYGNND
                     DRADNIAKWLVKTFYNKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPF
                     SPGANPAYGAEKNGALASLMSTAKIPYHYATDGISNTFGVTPNTLGHDDETRKDTLVHM
                     VDGYMENSGMHLNINVFNKETLIDAQKHPEEYPTLTVRVSGYCVYFADLTKEQQDDVIA
                     RTFFDEM"
ORIGIN
        1 cttaacaatc atgaagtttt aatctatgcc tccaatcatt acgaacccgc cacgttgcgt
       61 attggcaaac agacttacca cgtcgccaaa caagtcacca gttttccaaa tgcgcctaaa
      121 acgtttattc ctaacttgtt tgttgcgctc ccatctgatg tccaactatt aaccgcttta
      181 cacgcgcttt atccaaaaag gtcaatggca ctgctcaaag ccgacgtgac cactaatcaa
      241 gacgtaacat taagcggcag tcacgccaat caggtcaagc tttatcatgc agtcgtcaaa
      301 agagggattg cattaccgga atcggttcaa tcgcgagccg ctaattacga tgatactgcc
      361 agcatgatga gcagtttcct attccttggg gttgtctttg gggtgacctt catcctggca
      421 accttgctga ttctttacta caagcagttg gcagaaggct acgcggatgc acgccgctat
      481 accatcttac aacgcgtcgg cctgagcctt cgggaagttc gcgctaccat taatagtcag
      541 ctactcacac tgttttacct gcctttgatc gtcgctgcca tccacgtggg ctttgcattg
      601 ccctttgtcc aacgtattat gatgctattc ggcctgccgg attggcaatt cttcttaacc
      661 gtttcgttaa tcacgcttgc aatttttgcg gtcatctacg tgctgatgta ccgtttgacc
      721 ggcaacgttt attatcgaat tgtttcccgc cgtcagccat ccagtcgtgg gtgagggcgg
      781 tctgacgcat agtataaaaa agctgcctga tcgcaagcca tcaaaggcga tcaggcagct
      841 tttttctatg tcaaccagca tcaaaaaatg tcgccactgt tatctcactg aatactgtga
      901 cgttaattag cgccgcaaca tgacttaacc agacaactaa ccgccgacat tatggttgct
      961 ggggatgttg atccaaggct ttggcggcag tttccgaaac ttcgcgtaag gccgcttttc
     1021 cacttttatc gctgagattg atgtacagat ccgtcgcgct ggtcgtcgtt tgtccgagtt
     1081 gatcggcaac ttgctgcaca tccttggtga cggtataaat attggttccc aaggtatggc
     1141 gtgctttatg gggtgttgat gggcggccat aagcggttgt atagcgtttg aagaatgctt
     1201 caatcgtatt ttggcgaatg cggtttacca tttggccgat ttgcgtcaca aatagcggag
     1261 aagtaggctt ctgctgacca atcatcgcgg ttcgggactt caagtaagcc gttaaataag
     1321 gatcaatcca gtcagcaaag ttaacgcgat cctgaaaatt acctttacgc accacctgca
     1381 cagtatgacg atccatattg agatcttcca aattcatatt gaccaattca ctaacccgca
     1441 cgccggagcc aaacagcagc gcaatgatcg ccaaatttcg ttccttcgtc gcctcaaaat
     1501 aagcacgcgg tttcgatggc agctgctgag catattgctg atccagccac tgtaaaaagc
     1561 gcgcatcttc gccatccaca aacaacattt cctgcagttt atgattacgg taagtcgtcg
     1621 tctctgcatg ggtcttaagt ttcaccttat tccaaacatt gcggtaaaaa taaggctcac
     1681 cgtccgcatt tggttcgctt tcttcggtta agaaccgata aagggccttg atcccgacca
     1741 gtcgtaacgc cacactgcgc cgcgtcatac gtttattcgg atgggttttt gacggccgct
     1801 caagtaaata cgccacaaac gcctgcgcat cacgcaagga aagccgctct aaagtttcca
     1861 aaggcacatt ctgtggcgaa atggcgtccg tgacccgact ggagataagc caatgcagaa
     1921 attcctggta aaagtgcagg tactggtaaa gggtagaatc cgcgatggga attgcttttt
     1981 ggctgtgata gtaatcctga atatagtccg gcagggaagg tagcatggct tgtattaatt
     2041 taggatagtt ctgtgtcgac aaataaagtc acctcgaaca tatgttttct tttggataat
     2101 ttcagtatac gcgaaaaaac cagtgcgtgg cgagggataa tgacgaaaat atcggtaatt
     2161 cgcaacgata tgcgccgaaa aaagcaccag tgtagaactg aataaagttt tagtattatc
     2221 caaaggtaaa tgggcttcta atcatcaagc cagctaaatc accaaatttg ttgatctgtc
     2281 atttgacact aaacttagtt gcatttcaaa tttgaattta ctgcacgatc aggaccatct
     2341 gtgctgatgt cctgattcca cggcgccgct ttagcaacag tttaaccaat taaccaacga
     2401 ttcattggct gcaatcaaat caattttcgt attttgatcc agaattcagc tgacagcaac
     2461 acttgcgact tgaatgctgt ttatgactca aaatgatact ttctacaaat ctgctttgat
     2521 taatgctgaa tttacatata tttagcaacg actggtcatt ttcaaaccag aaaaaacaaa
     2581 taaagcgcac cgctcacatc cgtacaattg atttgcttct ctcaaaagtt tttgagctgt
     2641 ctgtcaggtg aggggacttg catcaatcgt tttgccattt gaacaccaaa taagtcacgt
     2701 caaagtcaac gactcaaaat ctccattcta gtttacataa tatatattat acgaagtagt
     2761 tatgaggctg cgttttttgc acctcgtttt aaatttgaac tatccttttt gttcatccaa
     2821 gtttttgagc acaaaaaaat cgcaaccaca aacggctgcg atttataaaa ccaattatta
     2881 agttcaaaat taaccttcaa acgcaccttg cattggcggt acgacctgct tcttacgtga
     2941 aaccacgcca ggcagtgcca accggtcatt tgcatcaaac ttttcgttga atgctgcggc
     3001 cactttgtca cgatctgcgc caacagcaaa gcccgttgaa ttgctgttca gaatatcggt
     3061 tgcaattaac aggaagtcat catagccctt cgcctgtgct tctttggtca tagcggccac
     3121 caattcatcc tgacgcgcat aaatatcatc aagatcaact gtattcactt gtccaatacg
     3181 gatagactta ccgcccatcg taaagctctt cgcatcgcct tcaatgatat ccatatccga
     3241 cttggcagct agattggtgc ccgccttcaa catagccaag ccatatgatt gcaggtcaat
     3301 gccagcgatt ttagccattt ccggcaagat ctttttgtcc ttgtcggttg cagtcggtga
     3361 cttcagaagc agggtatccg aaatcaagcc agaagccatt aagcctgcca gtgttggtga
     3421 aatttcatag ccgtgttcct ggaacaactc gaaaatcacc gtgttggtac tgcccactgg
     3481 ttctgcccgg taatatagtg gtgacgccgt atggaagttg gcaatgcggt ggtgatcaac
     3541 cactgaatcg atttgcacgt cttcgatatc agcaacgctt tgctggaatt cgttatgatc
     3601 aaccaacatt acgtgcccgg tttcatttgc ggctgtctta atgacccgcg gcgcagcaac
     3661 cttaaaatga tccaatacaa acttggtttc atcgtttggt tcaccgagtg ctactgcttc
     3721 aacatcggta ttacctgctt gcttctgaaa ttcagcccaa ctcatggcgc ttgtaatcgc
     3781 gtctgtatca gggttttgat gtccaaaaat caaagtcgtc atgttatctg atcgctcctc
     3841 agctgaatag tttaccgttc aattttacca ctgttttcgc tgacccgcga tagataatct
     3901 ttctcggata aatgtgcgtc aaacgtctta aagaagttgg caatccgcgg aatatcggct
     3961 ttttccttgc gatacacaaa cgaggtctca aagcgaatcg ctggttcaaa ggcaacggca
     4021 tgcgccttga tttgatcagc atgagcggca acaaaactgg ttggtaacgc ggtgttaaca
     4081 ccggtatggt tgacaaactg agttaactga tatggcgtgg taaaatgcgc cacaacatgc
     4141 ggaaaatcaa ccatctgatt cttatacgcc tcatgcaaca actgacccag gaaatagtcg
     4201 tctggataag ttacccacgg ctcagccagc gtatctttaa aatgcaggcg tttgcgatta
     4261 gccaaggaat tatcatgatg caggaacaat aactgttcat caagaatctt cttgcttgca
     4321 tacggcttcc agttcttaat cgtctcatca ggcagattca tgatggccaa atcaatctga
     4381 ttgttctcca acgcttccca gatctcatga cgtgtcatca ttcgtagcgt gatttcgact
     4441 tcaggattaa ggtcgtaata atgcgcgata aaagcattaa acgcactgtc ttcaattgaa
     4501 cttaacaagc caatattaat cttgccgact gtggcactgg tgctttgctg aatctcgtca
     4561 gtcgtctggt tcaaaatttc ataaatctcg tgtgtcgcct tcaacatcgt gcgaccggca
     4621 tcggataaat gcaacttctt accaacggaa taaaacaatg gtgcccccac tgttcgttcc
     4681 aatttcttga tttgctgcgt cagtgccggc tgggtaatcc ccaacaactg ggctgactgg
     4741 gtgtagttca tggtttctgc taattgcaaa aaatacgtga gagttttgga ggaaaaaatg
     4801 cgctcttgtt tcgtcttcat agggcggctt ccctttcgtt tataatttga taacgttact
     4861 caaccatcat cataagtggc atttaaaaaa aacgcaacgg atttgctgcg tttttaatcg
     4921 ttacgttaag tttcaactaa atcactgctt gttagctggc tgtggtcgcc aactttgcca
     4981 tccagtatag tcctcaacgt gtagcaagtt ctctgcattc ttgacccgat cagctgaagg
     5041 cggctcgata ccttccagcg gatacgtaat acctagttca tggtacttct taacccctaa
     5101 cgtatggtac ggaagaattt ccactttctg gaccacgcgt ttatccaaag tggcaatata
     5161 atctcccaag cgtttgagat aaggatcaaa gtcggttcgt tctggcacaa gtacgtggcg
     5221 aatccacata tcatcgccat gatccgacat atactgaacc atatccaaaa tgttttcgtt
     5281 tgggaaaccg gttagctttt tgtgttccgc ggaatcaatg tgcttgatgt caaccaacga
     5341 aatatccgtg acggccatca gtttctcaaa ttgaccaaac cacggctgat cacgggtgaa
     5401 tggaccaccc gaagtatcca aacaagttga gatacccagt tccttagctt tggtaaaaag
     5461 ttccaggata aaatcaattt ggacgagact ttcgccaccg ctacaagtaa tccccccttg
     5521 ttcgccccag aaggctttat atcgctgtgc atctgccagg atctgatcag cggtcatttc
     5581 ctcgcccaca ccgatattcc aagtgtcagg gttgtgacag tactggcacc gcatcctgca
     5641 tccttgcata aagacgacaa accgaatccc gggaccatca accgaaccaa agctttcgac
     5701 tgaatgaacg tatcctttta ttgggccttc atgatcctgt cccacttgtt ccaaaatatt
     5761 taacgggctt tgtgattgat cgatgagttt catctatgcc gcctccagtg caaataaata
     5821 cctaattttc aacgattttc atttcagttt gtcaaaaaac ggcaacagca tcgttgccgt
     5881 cttttgacaa aacaggaatg atctcctgtt ttgcgaatag attgacttga ttacatttcg
     5941 tcgaagaagg tccgagcgat aacatcgtct tgctgttctt tggtcaggtc tgcaaagtag
     6001 acgcagtaac cggaaactcg aacagtcaag gttggatatt cttctgggtg cttctgggca
     6061 tcaatcaacg tttctttatt gaagacgttg atgttaaggt gcatgccgct attttccatg
     6121 tagccgtcaa ccatgtgaac caaggtgtcc ttacgggttt catcgtcatg gcctaaggtg
     6181 ttcggtgtta ccccaaaggt attgctgatc ccgtctgttg catagtgata tgggatcttg
     6241 gcggttgaca tcaaggaagc caaagcaccg ttcttttcag caccatacgc cgggttggca
     6301 ccaggtgaga atggttcgcc cttctgacgg ccgtttggtg ttgtcccggt attcttacca
     6361 taaaccacat tagatgtaat ggtcagaaca ctggtactga gcttagcacc gcgatacaga
     6421 tgatgtgtat tcatcttgtt gtagaaggtc ttaaccaacc acttagcaat gttgtctgcc
     6481 cggtcatcat tgttaccata acgcggatag tcatcattgt cagcaacaaa gtcgattgca
     6541 acgccatttt catcccgaat tgccttcacg tggccatact tgatggcact gatggaatca
     6601 accgcatgtg acaaaccgga aatcccggta gcaaacgtcc gattcagacg tgtatccttc
     6661 aatgccaact gcgcagcttc atagtagtac ttgtcatgca tgtagtgaat aacattcagt
     6721 gcattgacat agacatctgc taaccagtcc atcatcttgt cgaacttctt catgaactcg
     6781 tcatagtcga tatagtcgct ggtgatcggc tcgtattcag gtcccacctg tgcttcctga
     6841 atttcgtctt taccaccgtt gatcgcatac agcacggtct tggctaggtt tgctcgcgcc
     6901 ccgaagtact gaacaccatc tgcaattggc tgcgcggaaa cacagcaagc aatgccgtaa
     6961 tagtcggttc cccattcgtt acgcatcaga tcatcgtttt cgtattgaat ggttgaactt
     7021 tggatggaca cttcagtggc ataacgtttg aacccttcag gaagacgttc gctccacaac
     7081 aaggtgatgt ttggttccgg tgccgcgccc atgttgtcca aggtcttcag gaagcggaac
     7141 gcggtcttgg taacatgatg acggccatca ttacccatcc ctgccaagga cagtgttgcc
     7201 cagatcggat cgccggagaa cagtgagttg tattcttcag tccgaatgaa acgtaccatc
     7261 cgtaacttca taaccaaatg atcgatgagt tcttgtgcct gctcttcatt cagcttgccc
     7321 aaacgcatat cccgttgaat ataggcatca atcactgtat cgatacggcc gacagacatt
     7381 gccgcaccat tttgggtctt aacggctgcc aggtaaccga agtagatcca ctgaacagct
     7441 tcttgagcat tagcagctgg gcgggaaatg tcatagccgt attctttggc catcttcttc
     7501 atgtc
//
