LOCUS       c00023_gnlPro..         7355 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AOGAALNK_26.
ACCESSION   c00023_gnlPro..
VERSION     c00023_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-30 04:28:42
            Original ID  :: gnl|Prokka|AOGAALNK_26
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7355
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7355
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3878:7129]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3879..7129
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7355
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7355
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..944)
                     /locus_tag="ctg23_1"
                     /transl_table=11
                     /translation="LSTQNYPKLIQAMLPSLPDYIQDYYHSQKAIPIADSTLYQYLHFY
                     QEFLHWLISSRVTDAISPQNVPLETLERLSLRDAQAFVTYLLERPSKTHPNKRMTRRSV
                     ALRLVGIKALYRFLTEESEPNADGEPYFYRNVWNKVKLKTHAETTTYRNHKLQKMLFVD
                     GEDARFLQWLDQQYAQQLPSKPRAYFEATKERNLAIIALLFGSGVRVSELVNMNLEDLN
                     MDRHTVQVVRKGNFQDRVNFAEWIDPYLTAYLKSRTAMIGHQKPTSPLFVTQIGQTVNR
                     IRQNTIEAFFKRYTTAYGRPSTPHKARHTLGTN"
     CDS             complement(1827..2759)
                     /locus_tag="ctg23_2"
                     /transl_table=11
                     /translation="MTTLIFGHQNPDTDAITSAMSWAEFQKQAGNTDVEAVALGEPNDE
                     TKFVLDHFKVAAPRVIKTAANETGHVMLVDHNEFQQSVADIEDVQIDSVVDHHRIANFH
                     TASPLYYRAEPVGSTNTVIFELFQEHGYEISPTLAGLMASGLISDTLLLKSPTATDKDK
                     KILPEMAKIAGIDLQSYGLAMLKAGTNLAAKSDMDIIEGDAKSFTMGGKSIRIGQVNTV
                     DLDDIYARQDELVAAMTKEAQAKGYDDFLLIATDILNSNSTGFAVGADRDKVAAAFNEK
                     FDANDRLTLPGVVSRKKQVVPPMQGAFEG"
     CDS             complement(2789..3757)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     55.5; E-value: 1.4e-17)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg23_3"
                     /sec_met_domain="LysR_substrate (E-value: 8.8e-28,
                     bitscore: 85.5, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 1.1e-18, bitscore: 55.5,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKQERIFSSKTLTYFLQLAETMNYTQSAQLLGITQPALTQQIK
                     KLERTVGAPLFYSVGKKLHLSDAGRTMLKATHEIYEILNQTTDEIQQSTSATVGKINIG
                     LLSSIEDSAFNAFIAHYYDLNPEVEITLRMMTRHEIWEALENNQIDLAIMNLPDETIKN
                     WKPYASKKILDEQLLFLHHDNSLANRKRLHFKDTLAEPWVTYPDDYFLGQLLHEAYKNQ
                     MVDFPHVVAHFTTPYQLTQFVNHTGVNTALPTSFVAAHADQIKAHAVAFEPAIRFETSF
                     VYRKEKADIPRIANFFKTFDAHLSEKDYLSRVSENSGKIER"
     CDS             complement(3879..4730)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 66.6;
                     E-value: 9e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg23_4"
                     /sec_met_domain="Radical_SAM (E-value: 2.4e-27, bitscore:
                     84.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLIDQSQSPLNILEQVGQDHEGPIKGYVHSVESFGSVDGPGIRF
                     VVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILADAQRYKAFWGEQGGITCSGGESLV
                     QIDFILELFTKAKELGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAE
                     HKKLTGFPNENILDMVQYMSDHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKRVVQ
                     KVEILPYHTLGVKKYHELGITYPLEGIEPPSADRVKNAENLLHVEDYTGWQSWRPQPAN
                     KQ"
     CDS             complement(4868..7129)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg23_5"
                     /sec_met_domain="Gly_radical (E-value: 2e-42, bitscore:
                     132.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.3e-245, bitscore:
                     804.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQLDETKVPNYWEGFNGGDWQEEINVRDFIEHNLNQYDGDESFL
                     AGPTEATTVLNNQVLNLKKQERANGGVLDADNNIPSTITSHGPGYLNKDLEKIVGVQTD
                     KPFKRAFMPFGGIRMAEDALKSYGFETDANEHKIFTEYRKTHNQGVFDAYTPEMRKARH
                     YKIVTGLPDAYGRGRIVSDFPRIAIYGIDRLMAEKFNDYNLTGDGEMTDDVIKLREEIN
                     EQYRALNDMKKMAKEYGYDISRPAANAQEAVQWIYFGYLAAVKTQNGAAMSVGRIDTVI
                     DAYIQRDMRLGKLNEEQAQELIDHLVMKLRMVRFIRTEEYNSLFSGDPIWATLSLAGMG
                     NDGRHHVTKTAFRFLKTLDNMGAAPEPNITLLWSERLPEGFKRYATEVSIQSSTIQYEN
                     DDLMRNEWGTDYYGIACCVSAQPIADGVQYFGARANLAKTVLYAINGGKDEIQEAQVGP
                     EYEPITSDYIDYDEFMKKFDKMMDWLADVYVNALNVIHYMHDKYYYEAAQLALKDTRLN
                     RTFATGISGLSHAVDSISAIKYGHVKAIRDENGVAIDFVADNDDYPRYGNNDDRADNIA
                     KWLVKTFYNKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPA
                     YGAEKNGALASLMSTAKIPYHYATDGISNTFGVTPNTLGHDDETRKDTLVHMVDGYMEN
                     SGMHLNINVFNKETLIDAQKHPEEYPTLTVRVSGYCVYFADLTKEQQDDVIARTFFDEM
                     "
ORIGIN
        1 atattggttc ccaaggtatg gcgtgcttta tggggcgttg atgggcggcc ataagcggtt
       61 gtatagcgtt tgaagaatgc ttcaatcgta ttttggcgaa tgcggttcac cgtttggccg
      121 atttgcgtca caaatagcgg agaagtaggc ttctggtgcc cgatcatcgc ggttcgggac
      181 ttcaaataag ccgttaaata aggatcaatc cattcagcaa agttaacgcg atcctgaaaa
      241 ttacctttac gcaccacctg cacagtatga cgatccatat tgagatcttc caaattcata
      301 ttgaccaatt cactaacccg cacgccggag ccaaacagca acgcaatgat cgccaaattt
      361 cgttccttcg tcgcctcaaa ataagcacgc ggtttcgatg gcagttgctg agcatattgc
      421 tgatccagcc actgtaaaaa gcgcgcatct tcgccatcca caaacaacat tttctgcagt
      481 ttatgattac ggtaagtcgt tgtctctgca tgggtcttaa gtttcacctt attccaaaca
      541 ttgcggtaaa aataaggctc accgtccgca tttggttcgc tttcttcggt taagaaccga
      601 taaagggcct tgatcccgac cagtcgtaac gcaacactgc gccgcgtcat gcgtttattc
      661 ggatgggttt tcgacggccg ctcaagtaaa tacgtcacaa acgcctgcgc atcacgcaag
      721 gaaagccgct ctaaggtttc caaaggcaca ttctgtggcg aaatggcgtc cgtgacccga
      781 ctggagataa gccaatgcag aaattcctgg taaaagtgca ggtactggta aagggtagaa
      841 tccgcgatgg gaattgcttt ttggctgtga tagtaatcct gaatatagtc cggcagggaa
      901 ggtagcatgg cttgtattaa tttaggatag ttctgtgtcg acaaataaag tcacctcgaa
      961 catatgtttt cttttggata atttcagtat acgcgaaaaa accagtgcgt ggcgagggat
     1021 aatgacgaaa atatcggtga ttcacaacga tatgcgccga aaaaagcacc ggtgtagaac
     1081 tgaataaagt tttagtatta tccaaaggta aatgggcttc taatcatcaa gccagctaaa
     1141 tcaccaaatt tgttgatctg tcatttgaaa ctaaacttag ttgcatttca aatttgaatt
     1201 tactgcacga tcagaacatc tgtgctgatg tcctgattcc acggcgccgc tttagcaaca
     1261 gtttaaccaa cgattcattg gctgcaatca aatcaatttt cgtattttga tccagaattc
     1321 agctgacagc aacatttgcg acttgaatgc tgtttatgac tcaaaatgat actttctaca
     1381 aaactgcgtt gattgatgct gaatttacaa atatttagca acgactggtc attttcaaac
     1441 cagaaaaagc aaataaagcg caccgctcac atccgtacaa ttgatttgct tctctcaaaa
     1501 gtttttgagc tgtctgtcag gtgaggggac ttgcatcaat cgttttgcca tttgaacacc
     1561 aaataagtca cgtcaaagtc aacgactcaa aatctccatt ctagtttaca taatatatat
     1621 tatacgaagt agttataagg ctgctttttt tcacctcgtt ttaaatttga accatcctta
     1681 ctgaactgaa agatttcgcc aatcatcaca tttttgcacc ttcagatctg aaaattcgat
     1741 attttatgtt tatcaaagtt tttgagcaca aaaaaatcgc aaccacaaac ggctgcgatt
     1801 tataaaacca attattaagt tcaaaattaa ccttcaaacg caccttgcat tggcggtacg
     1861 acctgcttct tacgtgaaac cacgccaggc agtgtcaacc ggtcattcgc atcaaacttt
     1921 tcgttgaatg ctgcggccac tttgtcacga tctgcgccaa cagcaaagcc cgttgaattg
     1981 ctgttcagaa tatcggttgc aattaacagg aagtcatcat agcccttcgc ctgtgcttct
     2041 ttggtcatag cggccaccaa ttcatcctga cgcgcataaa tatcatcaag atcaactgta
     2101 ttcacttgtc caatacggat agacttaccg cccatcgtaa agctcttcgc atcgccttca
     2161 atgatgtcca tatccgactt ggcggctaaa ttagtgcccg ccttcaacat agccaaaccg
     2221 tatgattgca ggtcaatgcc agcgatttta gccatttccg gcaagatctt tttgtccttg
     2281 tcggtagcag tcggtgactt cagcaataac gtatccgaaa tcaagccaga cgccattaag
     2341 cctgccagtg tcggtgaaat ttcatagccg tgttcctgga acaactcgaa aatcaccgtg
     2401 ttggtactgc ccactggttc tgcccggtaa tatagcggtg acgccgtatg gaagttggca
     2461 atgcggtggt gatcaaccac tgagtcgatt tgtacatctt caatatcagc aacgctttgc
     2521 tggaattcgt tatgatcaac cagcattacg tgaccggttt cgtttgcagc tgtcttaata
     2581 acccgcggcg cagcaacctt aaaatgatcc aatacaaact tggtttcatc gtttggttca
     2641 ccgagtgcta ctgcttcaac atcggtatta cctgcctgct tctgaaattc agcccaactc
     2701 atggcgcttg taatcgcgtc tgtatcaggg ttttgatgtc caaaaatcaa agtcgtcatg
     2761 ttatctgatc gctcctcagc tgaatagttt accgttcaat tttaccactg ttttcgctga
     2821 cccgcgatag ataatctttc tcggataaat gcgcgtcaaa cgtcttaaag aagttggcaa
     2881 tccgcggaat atcagctttt tccttgcgat acacaaacga ggtctcaaag cgaatcgctg
     2941 gttcaaaggc aacggcatgc gccttgattt gatcagcatg agcggcaaca aaactggttg
     3001 gtaacgcggt gttaacgccg gtatggttga caaactgagt tagctgatac ggcgtggtaa
     3061 aatgcgccac aacatgcgga aaatcaacca tctgattctt atatgcctca tgcaacaact
     3121 gacccaggaa atagtcgtct ggataagtta cccacggctc agccagcgta tctttgaaat
     3181 gcaggcgttt gcgattagcc aaggaattat catgatgcag gaacaataac tgttcatcaa
     3241 gaatcttctt gctggcatac ggcttccagt tcttaatcgt ctcatcaggc aggttcatga
     3301 tggccaaatc aatctggttg ttctccaacg cttcccagat ctcatgacgt gtcatcattc
     3361 gcaacgtgat ttcgacttca ggattaagat cataataatg cgcgataaaa gcattaaacg
     3421 cactgtcttc aattgaactt aacaagccaa tattaatctt accgactgtg gcactggtac
     3481 tttgctgaat ctcgtcagtc gtctggttca aaatttcata aatctcgtgt gtcgccttca
     3541 acatcgtgcg accggcatcg gataaatgca acttcttacc aacggaataa aacaatggtg
     3601 cccccactgt tcgttccaat ttcttgattt gctgcgtcag tgccggctgg gtaattccca
     3661 acaactgggc tgactgggtg tagttcatgg tttctgctaa ttgcaaaaaa tacgtgagag
     3721 ttttggagga aaaaatgcgc tcttgtttcg tcttcatagg gcggcttccc tttcgtttat
     3781 aatttgataa cgttactcaa ccatcatcat aagtggcatt taaaaaaaac gcaacggatt
     3841 tgctgcgttt ttaatcgtta cgttaagttt caactaaatc actgcttgtt agctggctgc
     3901 ggtcgccaac tttgccatcc agtatagtcc tcaacgtgta gcaagttctc tgcattcttg
     3961 acccgatcag ctgaaggcgg ctcaatacct tccagcggat acgtaatacc tagttcatgg
     4021 tacttcttaa cccctaacgt atgatacgga agaatttcca ctttctgaac cacgcgttta
     4081 tccaaagtgg caatataatc tcccaagcgt ttgagataag gatcaaagtc ggttcgttct
     4141 ggcacaagta cgtggcgaat ccacatatca tcgccatgat ccgacatata ctgaaccata
     4201 tccaaaatgt tttcgtttgg gaaaccggtc agctttttgt gttccgcgga atcaatgtgc
     4261 ttgatatcaa ccaacgaaat atccgtgacg gccatcagtt tctcaaattg accaaaccac
     4321 ggctgatcgc gggtgaatgg accacccgaa gtatccaaac aagttgagat acccagttcc
     4381 ttagctttgg taaaaagttc caggataaaa tcaatttgga cgagactttc gccaccgcta
     4441 caagtaatcc ccccttgttc accccagaag gctttatatc gctgtgcatc tgccaggatc
     4501 tgatcagcgg tcatttcctc gcccacaccg atattccaag tgtcagggtt gtgacagtac
     4561 tggcaccgca tcctgcatcc ttgcataaag acgacaaacc gaatcccggg accatcaacc
     4621 gaaccaaagc tttcgactga atgaacgtat ccctttattg ggccttcatg atcctgtccc
     4681 acttgttcca aaatatttaa cgggctttgt gattgatcga tgagtttcat ctatgccgcc
     4741 tccagtgcaa ataaatacct aattttcaac gattttcatt tcagtttgtc aaaaaacggc
     4801 aacagcatcg ttgccgtctt ttgacaaaac aggaatgatc tcctgttttg cgaatagatt
     4861 gacttgatta catttcgtcg aagaaggtcc gagcgataac atcgtcttgc tgttctttgg
     4921 tcaggtctgc aaagtagacg cagtaaccgg aaactcgaac agtcaaggtt ggatattctt
     4981 ctgggtgctt ctgggcatca atcaacgttt ctttattgaa gacgttgatg ttaaggtgca
     5041 tgccgctatt ttccatgtag ccgtcaacca tgtgaaccaa ggtgtcctta cgggtttcat
     5101 cgtcatggcc taaggtgttc ggtgttaccc caaaggtatt gctgatccca tctgttgcat
     5161 agtgatatgg gatcttggcg gttgacatca aggaagctaa agcaccgttc ttttcagcgc
     5221 catacgctgg gttggcacca ggtgagaatg gttcgccctt ctgacggccg tttggtgttg
     5281 tcccggtatt cttaccataa accacatttg atgtaatggt cagaacactg gtactgagct
     5341 tagcaccgcg atacagatga tgtgtattca tcttgttgta gaaggtctta accaaccact
     5401 tagcaatgtt gtctgcccgg tcatcattgt taccataacg cggatagtca tcattgtcag
     5461 caacaaagtc gattgcaacg ccattttcat cccgaattgc cttcacatgg ccatacttga
     5521 tggcactgat ggaatcaacc gcatgtgaca aaccggaaat cccggtagca aacgtccgat
     5581 tcagacgcgt gtctttcaat gccaactgtg cagcttcata gtagtacttg tcatgcatgt
     5641 agtgaataac attcagtgca ttgacataga catctgctaa ccagtccatc atcttgtcga
     5701 acttcttcat gaactcgtca tagtcgatat agtcgctggt gatcggctcg tattcaggtc
     5761 ccacctgtgc ttcctgaatt tcgtctttac caccgttgat cgcatacagc acggtcttgg
     5821 ctaggtttgc tcgcgccccg aagtactgaa cgccatctgc aattggctgt gcggaaacac
     5881 agcaagcaat gccgtaatag tcggttcccc attcgttgcg catcagatca tcgttttcgt
     5941 actgaatggt tgaactttga atggacactt cagtagcata acgtttgaac ccttcaggaa
     6001 gacgttcgct ccacaacaag gtgatgtttg gttccggtgc cgcgcccatg ttgtccaagg
     6061 tcttcaggaa gcggaacgcg gtcttggtaa catgatgacg gccatcatta cccatccctg
     6121 ccaaggacag tgttgcccag atcggatcgc cggagaacag tgagttgtat tcttcagtcc
     6181 gaatgaaacg aaccatccgc aacttcatca ccaaatgatc gatgagttct tgtgcctgct
     6241 cttcattcag cttgcccaaa cgcatatccc gttgaatata ggcatcaatc actgtatcga
     6301 tacggccgac agacattgcc gcaccatttt gggtcttaac ggctgccagg taaccgaagt
     6361 agatccactg aacagcttct tgagcattag cagctgggcg ggaaatgtca tagccgtatt
     6421 ctttggccat cttcttcatg tcattcaagg cacgatactg ttcgttaatt tcttcgcgca
     6481 gtttgataac atcatctgtc atctcgccat cgccggtcag gttgtaatcg ttgaacttct
     6541 cagccatcag ccggtcaatc ccataaatag caattcgagg gaagtccgaa acgatccgac
     6601 cacgaccata agcatccggt aagccggtca caatcttata gtggcgggct ttgcgcattt
     6661 ccggcgtata agcatcaaaa acaccttggt tatgcgtctt acgatattcg gtaaagatct
     6721 tgtgctcatt agcgtcggtt tcgaagccat aggatttcaa agcatcctca gccatccgga
     6781 taccgccaaa cggcatgaat gctcgcttaa acggcttatc ggtttggaca ccgacaatct
     6841 tttcgaggtc cttattcaga taaccggggc catgagaagt aatggtcgat ggaatattgt
     6901 tatctgcatc cagtacaccg ccattggcac gttcttgctt tttcagattc aaaacctgat
     6961 tatttaaaac agtcgttgct tcagtggggc cagcaaggaa actctcgtcc ccatcatatt
     7021 ggttcaggtt atgctcgata aaatcgcgca cgttaatttc ttcttgccag tcgccgccgt
     7081 taaagccttc ccaataatta ggaactttgg tttcgtcaag ctgtttcata ggtattgcct
     7141 cctaaagtga attacagatg tgacagttct tacaaactca gtataacacg aaggcaaatc
     7201 tatgcaagcg ttttatcgct gatataacag gcgttttgct gtttttttgg tgtactttcc
     7261 tgtttcataa aaaaagctta atcattagct tgataagttg tgcacaactg tactgtcata
     7321 taataaataa aaaattctcc atctgtatta ataca
//
