LOCUS       c00099_gnlPro..         6528 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MOKFDKGI_113.
ACCESSION   c00099_gnlPro..
VERSION     c00099_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-29 04:55:23
            Original ID  :: gnl|Prokka|MOKFDKGI_113
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6528
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..810)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 66.8;
                     E-value: 8e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg99_1"
                     /sec_met_domain="Radical_SAM (E-value: 1.7e-27, bitscore:
                     85.0, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLIDQSQSPLNILEQVGQDHEGPIKGYVHSVESFGSVDGPGIRF
                     VVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILADAQRYKAFWGEQGGITCSGGESLV
                     QIDFILELFTKAKELGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAE
                     HKKLTGFPNENILDMVQYMSDHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKRVVQ
                     KVEILPYHTLGVKKYHELGITYPLEGIEPPSADRVKNAENLLHVEDYT"
     protocluster    1..6528
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:3209]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..3209
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6528
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6528
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(948..3209)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg99_2"
                     /sec_met_domain="Gly_radical (E-value: 3.4e-42, bitscore:
                     131.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1e-245, bitscore:
                     804.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQLDETKVPNYWEGFNGGDWQEEINVRDFIEHNLNQYDGDESFL
                     AGPTEATTVLNNQVLNLKKQERANGGVLDADNNIPSTITSHGPGYLNKDLEKIVGVQTD
                     KPFKRAFMPFGGIRMAEDALKSYGFETDANEHKIFTEYRKTHNQGVFDAYTPEMRKARH
                     YKIVTGLPDAYGRGRIVSDFPRIAIYGIDRLMAEKFNDYNLTGDGEMTDDVIKLREEIN
                     EQYRALNDMKKMAKEYGYDISRPAANAQEAVQWIYFGYLAAVKTQNGAAMSVGRIDTVI
                     DAYIQRDMRLGKLNEEQAQELIDHLVMKLRMVRFIRTEEYNSLFSGDPIWATLSLAGMG
                     NDGRHHVTKTAFRFLKTLDNMGAAPEPNITLLWSERLPEGFKRYATEVSIQSSTIQYEN
                     DDLMRNEWGTDYYGIACCVSAQPIADGVQYFGARANLAKTVLYAINGGKDEIQEAQVGP
                     EYEPITSDYIDYDEFMKKFDKMMDWLADVYVNALNVIHYMHDKYYYEAAQLALKDTRLN
                     RTFATGISGLSHAVDSISAIKYGHVKAIRDENGVAIDFVADNDDYPRYGNNDDRADNIA
                     KWLVKTFYNKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPFSPGANPA
                     YGAEKNGALASLISTAKIPYHYATDGISNTFGVTPNTLGHDDETRKDTLVHMVDGYMEN
                     SGMHLNINVFNKETLIDAQKHPEEYPTLTVRVSGYCVYFADLTKEQQDDVIARTFFDEM
                     "
     CDS             complement(3454..5895)
                     /locus_tag="ctg99_3"
                     /transl_table=11
                     /translation="LANQIQELTLEEVMGDRFGRYSKYIIQERALPDVRDGLKPVQRRI
                     LFAMNQDGNTYDKGFRKSAKSVGNVMGNYHPHGDSSIYEAMVRLSQDWKLRAPLIQMHG
                     NNGSMDGDPPAAMRYTEARLSKISKEMLADIDKETVDMVLNFDDTAQEPTVLPAGFPNL
                     LVNGATGISAGYATEIPPHNLREVVNAILYLLKHPQADLADLMQYVKGPDFPTGGIIQG
                     LDGIKQAYETGRGKIVVRSRTHIETIRGGREKIVVTEIPYDVNKAQMIKKIDELRLNKK
                     VDGIAEVRDESDRFGLSVVIELKKEADAHGILNYLFKNTDLQITYNFNMVAIADMQPKL
                     LGLKAMLEAYVAHRRDVVTRRTRYELNKAQARQHIVEGLIKMLSILDQVIAAIRASSDK
                     GDAKRNLVKQFDFSEAQAEAIVSLQLYRLTNTDITALQDEAAQLAKAIAEYQDILAQPA
                     SRDRVIEKELKRIAKEYGDDRRSSIQAEIETLEVATTVTVADETVMVQVSRDGYVKRSS
                     LRSYQAVDPEDNGLKPNDLAIFTGELSTLQHLYIVTNAGNIIYRPVYEISDARWKDTGE
                     HLSQTVGLGTDEKVLAVFAFDQLDLAGTFVLGSSDGYIKQTALSDLQPQRTYKRKPMMA
                     MKLKTPGAVVTNAYFTTDQAQDVFVVSRHAYGLDFPLAEVSTVGARATGVKSMDLKPED
                     EVVNFILVKQPEKAVVGILTQRGAFKRMALSEVGQMSRARRGLLVLRELKRDPHRIVAM
                     MQVDDNTRLDVLTDADKVITLTPVNHPTGDRYSNGSFVIDTDVQGKPVFVRTREETPVE
                     P"
     CDS             complement(5902..6528)
                     /locus_tag="ctg99_4"
                     /transl_table=11
                     /translation="AAGYVKNEEINTMIYTIGAGVGADFDIQQANYNKIIIMTDADTDG
                     AHIQILLLTFFYRYMRPLIDAGRVYIALPPLYRLQKGAGAKTKISYAWTDEELAKETKA
                     MGKGYTLTRFKGLGEMNADQLWETTMNPETRTLIRVRIDDAQLAERRVTTLMGDRVEPR
                     REWIEHHVKFDLDDEGSILETPTANHEKLQFGAKQIVKQLDKKRK"
ORIGIN
        1 agtatagtcc tcaacgtgta gcaagttctc tgcattcttg acccgatcag ctgaaggcgg
       61 ctcgatacct tccagcggat acgtaatacc tagttcatgg tacttcttaa cccctaacgt
      121 atggtacgga agaatttcca ctttctggac cacgcgttta tccaaagtgg caatataatc
      181 tcccaagcgt ttgagataag gatcaaagtc ggttcgttct ggcacaagta cgtggcgaat
      241 ccacatatca tcgccatgat ccgacatata ctgaaccata tccaaaatgt tttcgtttgg
      301 gaaaccggtt agctttttgt gttccgcgga atcaatgtgc ttgatgtcaa ccaacgaaat
      361 atccgtgacg gccatcagtt tctcaaattg accaaaccac ggctgatcac gggtgaatgg
      421 accacccgaa gtatccaaac aagttgagat acccagttcc ttagctttgg taaaaagttc
      481 caggataaaa tcaatttgga cgagactttc gccaccgcta caagtaatcc ccccttgttc
      541 gccccagaag gctttatatc gctgtgcatc tgccaggatc tgatcagcgg tcatttcctc
      601 gcccacaccg atattccaag tgtcagggtt gtgacagtac tggcaccgca tcctgcatcc
      661 ttgcataaag acgacaaacc gaatcccggg accatcaacc gaaccaaagc tttcgactga
      721 atgaacgtat ccttttattg ggccttcatg atcctgtccc acttgttcca aaatatttaa
      781 cgggctttgt gattgatcga tgagtttcat ctatgccgcc tccagtgcaa ataaatacct
      841 aattttcaac gattttcatt tcagtttgtc aaaaaacggc aacagcatcg ttgccgtctt
      901 ttgacaaaac aggaatgatc tcctgttttg cgaatagatt gacttgatta catttcgtcg
      961 aagaaggtcc gagcgataac atcgtcttgc tgttctttgg tcaggtctgc aaagtagacg
     1021 cagtaaccgg aaactcgaac agtcaaggtt ggatattctt ctgggtgctt ctgggcatca
     1081 atcaacgttt ctttattgaa gacgttgatg ttaaggtgca tgccgctatt ttccatgtag
     1141 ccgtcaacca tgtgaaccaa ggtgtcctta cgggtttcat cgtcatggcc taaggtgttc
     1201 ggtgttaccc caaaggtatt gctgatcccg tctgttgcat agtgatatgg gatcttggcg
     1261 gttgatatca aggaagccaa agcaccgttc ttttcagcac catacgccgg gttggcacca
     1321 ggtgagaatg gttcgccctt ctgacggccg tttggtgttg tcccggtatt cttaccataa
     1381 accacattag atgtaatggt cagaacactg gtactgagct tagcaccgcg atacagatga
     1441 tgtgtattca tcttgttgta gaaggtctta accaaccact tagcaatgtt gtctgcccgg
     1501 tcatcattgt taccataacg cggatagtca tcattgtcag caacaaagtc gattgcaacg
     1561 ccattttcat cccgaattgc cttcacgtgg ccatacttga tggcactgat ggaatcaacc
     1621 gcatgtgaca aaccggaaat cccggtagca aacgtccgat tcagacgtgt atccttcaat
     1681 gccaactgcg cagcttcata gtagtacttg tcatgcatgt agtgaataac attcagtgca
     1741 ttgacataga catctgctaa ccagtccatc atcttgtcga acttcttcat gaactcgtca
     1801 tagtcgatat agtcgctggt gatcggctcg tattcaggtc ccacctgtgc ttcctgaatt
     1861 tcgtctttac caccgttgat cgcatacagc acggtcttgg ctaggtttgc tcgcgccccg
     1921 aagtactgaa caccatctgc aattggctgc gcggaaacac agcaagcaat gccgtaatag
     1981 tcggttcccc attcgttacg catcagatca tcgttttcgt attgaatggt tgaactttgg
     2041 atggacactt cagtggcata acgtttgaac ccttcaggaa gacgttcgct ccacaacaag
     2101 gtgatgtttg gttccggtgc cgcgcccatg ttgtccaagg tcttcaggaa gcggaacgcg
     2161 gtcttggtaa catgatgacg gccatcatta cccatccctg ccaaggacag tgttgcccag
     2221 atcggatcgc cggagaacag tgagttgtat tcttcagtcc gaatgaaacg taccatccgt
     2281 aacttcataa ccaaatgatc gatgagttct tgtgcctgct cttcattcag cttgcccaaa
     2341 cgcatatccc gttgaatata ggcatcaatc actgtatcga tacggccgac agacattgcc
     2401 gcaccatttt gggtcttaac ggctgccagg taaccgaagt agatccactg aacagcttct
     2461 tgagcattag cagctgggcg ggaaatgtca tagccgtatt ctttggccat cttcttcatg
     2521 tcattcaagg cacgatactg ttcgttaatt tcttcgcgca gtttgataac atcatctgtc
     2581 atctcgccat cgccggtcag gttgtaatcg ttgaacttct cagccatcag ccggtcaatc
     2641 ccataaatag caattcgagg gaagtccgaa acgatccgac cacgaccata agcatccggt
     2701 aagccggtca caatcttata gtggcgggct ttgcgcattt ccggcgtata agcatcaaaa
     2761 acaccttggt tatgcgtctt acgatattcg gtaaagatct tgtgctcatt agcgtcggtt
     2821 tcgaagccat aggatttcaa agcatcctca gccatccgga taccgccaaa tggcatgaat
     2881 gcccgcttaa acggcttatc ggtttggaca ccgacaatct tttcgaggtc cttattcaga
     2941 taacctgggc catgagaagt aatggtcgat ggaatattgt tatctgcatc cagcacgccg
     3001 ccattggcac gttcttgctt tttcagattc aaaacctgat tatttaaaac ggtcgttgct
     3061 tcagtggggc cagcaaggaa actctcgtcc ccatcatatt ggttcaggtt atgctcgata
     3121 aaatcgcgca cgttaatttc ttcttgccag tcgccgccgt taaagccttc ccaataatta
     3181 ggaactttgg tttcgtcaag ctgtttcata ggtattgcct cctaaagtga attacagatg
     3241 tgacagttct tacaaactca gtataacacg aaggcaaatc tatgcaaacg ttttatcgct
     3301 gatataacag gcgttttgct gttttttcgg tgtactttcc tgtttcataa aaaaagctta
     3361 atcattagct tgataagttg tgcacaaccg tactgtcata taataaatca aaaagtcctc
     3421 atctgtatta atacagatga ggactttttt gcgttacggt tcaaccggtg tttcttcacg
     3481 tgtgcgtacg aaaaccggtt ttccttggac atcagtatca atcacaaagg aaccattaga
     3541 gtagcgatca cctgtcgggt ggttcacagg cgttaacgtg atcactttat cggcatcggt
     3601 taacacatcc agacgcgtgt tgtcatcgac ttgcatcatg gcgacaatgc ggtgcggatc
     3661 acgctttaat tcgcgtaaaa caagcaatcc gcggcgagca cggctcattt gaccgacttc
     3721 gcttaacgcc atacgtttaa acgcaccacg ctgagtcaaa atgcccacca ctgctttttc
     3781 tggttgctta actaaaataa agttaactac ctcatcttcc ggtttgagat ccatactttt
     3841 gaccccagta gcccgagcac cgaccgtgga aacttcagcc agtgggaagt cgagtccata
     3901 cgcatgtcgg ctgacaacaa aaacatcctg ggcttgatcg gtcgtgaagt aagcattggt
     3961 aaccacggca cccggtgttt tcaatttcat tgccatcatt ggtttgcgtt tatacgtgcg
     4021 ctgcggctgc aaatcactca atgccgtctg cttaatgtag ccgtcactgg agccaaggac
     4081 aaaggtacca gcaagatcta gctgatcaaa agcaaagact gccaaaacct tttcatcggt
     4141 tcccaagcca actgtctggc ttaaatgttc cccagtgtcc ttccaccgcg catcgctaat
     4201 ttcgtaaacg ggccgataaa tgatattacc ggcattcgtg acaatgtaaa gatgttgcag
     4261 ggtgcttaac tctccggtaa aaatcgccaa atcattgggt tttaaaccgt tatcttcagg
     4321 atcaacagct tggtaactac gcaaggagct gcgtttgaca tagccgtcgc gtgacacctg
     4381 gaccatcacg gtttcatcag ccactgtgac cgtcgttgcc acttcgagcg tttcaatctc
     4441 ggcttgaata ctgctgcgcc gatcatcccc gtactcttta gcaatccgtt taagttcttt
     4501 ttcaatcacc cgatcacgcg aagccggctg cgctagaatg tcttgatatt cggcaatcgc
     4561 cttggccagt tgtgccgcct cgtcctgcaa agcggtaatg tcggtattcg tcaaccgata
     4621 taactgcaag gagacaatgg cttcagcctg cgcttcggaa aaatcaaatt gtttgaccaa
     4681 attgcgtttg gcatcccctt tatctgagga tgcacgaatc gccgcaatga cttgatccaa
     4741 aatggacaac attttaatca agccttcaac aatgtgctgc cgtgcctgcg ctttgtttaa
     4801 ctcataacgt gtccggcgtg tgaccacatc acggcgatgc gccacataag cttccaacat
     4861 cgccttgagc ccaagcaatt tcggttgcat atcggcaatc gcaaccatat taaagttata
     4921 tgtgatttgc agatcggtat ttttgaagag gtaattcaaa attccatggg catccgcttc
     4981 ttttttcagc tcaatgacaa cacttaagcc gaatcgatca ctttcatcgc gaacttcagc
     5041 gataccgtcg acttttttgt taagtcgtag ttcatcgatt tttttaatca tttgggcctt
     5101 gttgacatca tacggaattt cagtgaccac aatcttctcg cggccgccgc gaatggtttc
     5161 gatgtgggtg cgtgagcgaa cgacaatctt tccccggccg gtttcgtatg cctgcttgat
     5221 cccgtcaagc ccttggatga tgccgccggt tggaaaatcg ggtcccttaa catactgcat
     5281 caagtcggct aagtcagctt gcggatgttt tagcaaatac aagatagcat tgacgacttc
     5341 ccgcaaatta tgcggcggga tttcggtcgc ataccctgca gagatcccgg tagcaccgtt
     5401 gacgaggaga ttaggaaaac ccgctggcag tacagtcggt tcctgggcag tgtcatcaaa
     5461 gtttaaaacc atatcaacgg tttctttatc aatatccgcc agcatttcct tactgatctt
     5521 gcttagccgc gcttctgtat aacgcatcgc tgctggcgga tccccatcca ttgacccatt
     5581 gttaccgtgc atctgaatta acggtgcgcg tagtttccag tcttggctga gccgcaccat
     5641 cgcttcgtaa atcgagctat cgccatgagg atgataatta cccataacat taccgacact
     5701 cttagctgac ttgcgaaatc ctttgtcata agtgttgccg tcttggttca tcgcaaataa
     5761 aatgcgtcgt tggaccggtt ttaacccgtc acgaacatct ggcagtgcgc gttcttgaat
     5821 aatatatttc gagtaccggc caaaccggtc gcccatgacc tcttctaagg tcaactcttg
     5881 aatctgattg gccaatggct tttacttcct tttcttatct aattgcttga cgatttgctt
     5941 agctccgaat tgcaactttt catggtttgc cgttggagtt tccagaatac tgccttcatc
     6001 atccagatca aacttcacgt gatgttcaat ccattcacgc cgcggttcaa cgcgatcgcc
     6061 catcaaagtc gtaacccggc gttctgccag ctgcgcatcg tcaatccgaa cccgaatcag
     6121 tgtccgcgtt tcgggattca tcgtggtctc ccatagttga tcggcgttca tttcgccaag
     6181 ccccttaaag cgggtcaagg tatagccttt gcccatcgcc ttggtttcct tagctagctc
     6241 ttcatccgtc cacgcatagc tgatcttcgt tttagcgcca gcaccttttt gcaagcggta
     6301 caacggcggc agtgcgatat acacacgccc ggcatcgatt aacggccgca tgtatcggta
     6361 aaagaaggtc aacagcagga tctgaatgtg cgcaccatca gtatcggcat cggtcataat
     6421 aatgatctta ttgtagttag cctgctggat gtcaaaatcg gcaccgacac cggcgccaat
     6481 ggtataaatc atggtattga tttcttcgtt tttgacgtat cctgcagc
//
