Select genomic region:
Overview
1.1
1.2
1.3
1.4
1.5
1.6
1.7
1.8
1.9
1.10

### Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaANBMDGDF_1 (original name was: gnl|Prokka|ANBMDGDF_1)

Region | Type | From | To | Most similar known cluster | Similarity
--- | --- | --- | --- | --- | ---
Region 1 | lanthipeptide-class-iii,NRPS,transAT-PKS | 193,327 | 277,134 | andalusicin A/andalusicin B | RiPP:Lanthipeptide | 100%
Region 2 | NRPS | 345,741 | 411,151 | surfactin | NRP:Lipopeptide | 82%
Region 3 | PKS-like | 924,760 | 966,004 | butirosin A/butirosin B | Saccharide | 7%
Region 4 | terpene | 1,050,778 | 1,071,521 |
Region 5 | transAT-PKS,T3PKS,NRPS | 1,624,692 | 1,734,781 | bacillaene | Polyketide+NRP | 100%
Region 6 | NRPS,transAT-PKS,betalactone | 1,795,152 | 1,880,708 | fengycin | NRP | 86%
Region 7 | terpene | 1,924,757 | 1,946,640 |
Region 8 | T3PKS | 2,001,008 | 2,042,108 |
Region 9 | NRP-metallophore,NRPS | 2,801,341 | 2,853,073 | bacillibactin | NRP | 100%
Region 10 | other | 3,358,364 | 3,399,782 | bacilysin | Other | 100%

gnlProkkaANBMDGDF_1 - Region 1 - NRPS,lanthipeptide-class-iii,transAT-PKS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 193,327 - 277,134 nt. (total: 83,808 nt) Show pHMM detection rules used
lanthipeptide-class-iii: (micKC and not PKS_KS)
NRPS: cds(Condensation and (AMP-binding or A-OX))
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
NRPS/PKS domains
KnownClusterBlast
Lanthipeptides
NRPS/PKS modules
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A domain glossary is available here, and an explanation of the visualisation is available here.
Selected features only
Show module domains
Similar known gene clusters from MIBiG 3.1
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.
Lanthipeptide predictions
Shows the possible core peptides for each biosynthetic enzyme.
Includes CompaRiPPson results for any available databases, with a detailed explanation here.

#### Lanthipeptide(s) for ctg1_192

ctg1_193
Leader:
MNDVLELQKLEKETDGKGIAV
Core:
D A T I T T T W T V T T T S G F V S S V S N H C
Core with dehydrations:
D A Dhb I Dhb Dhb Dhb W Dhb V Dhb Dhb Dhb Dha G F V Dha Dha V Dha N H Cys

#### MIBiG 3.1 matches for ctg1_193

37.5% BGC0002111 (EVG22_32245): Lanthipeptide: andalusicin A or andalusicin B
9
T V T T T S G F V S S V S N H C
24
T V T T T + S++S N+C
8
T V T T T G A W A S T I S N N C
23
37.5% BGC0002111 (multiple genes): Lanthipeptide: andalusicin A or andalusicin B
9
T V T T T S G F V S S V S N H C
24
T V T T T + S++S N+C
8
T V T T T G V W A S T I S N N C
23

#### antiSMASH-DB 4.0 matches for ctg1_193

100.0% NC_022081.1 and 19 others:
NZ_CP016913.1 Lanthipeptides: BARD7_RS19060_lanthipeptide NZ_CP022654.2 Lanthipeptides: CHN56_RS21570_lanthipeptide NZ_CP032144.1 Lanthipeptides: D3C60_RS19850_lanthipeptide NZ_CP034203.1 Lanthipeptides: EBA29_RS19705_lanthipeptide NZ_CP040378.1 Lanthipeptides: D1120_RS19620_lanthipeptide NZ_CP068563.1 Lanthipeptides: JKJ03_RS01160_lanthipeptide NZ_CP069391.1 Lanthipeptides: JR311_RS02265_lanthipeptide NZ_CP073635.1 Lanthipeptides: KEF49_RS01155_lanthipeptide NZ_CP083764.1 Lanthipeptides: LAZ98_RS01175_lanthipeptide NZ_CP085388.1 Lanthipeptides: LIS75_RS01165_lanthipeptide NZ_CP088300.1 Lanthipeptides: LQ763_RS01075_lanthipeptide NZ_CP097586.1 Lanthipeptides: M8971_RS04245_lanthipeptide NZ_CP107556.1 Lanthipeptides: OF857_RS01165_lanthipeptide NZ_CP116014.1 Lanthipeptides: PF996_RS01170_lanthipeptide NZ_JAGFPJ010000003.1 Lanthipeptides: J5M77_RS15590_lanthipeptide NZ_JAQEMJ010000010.1 Lanthipeptides: PGW53_RS13095_lanthipeptide NZ_LMAG01000010.1 Lanthipeptides: ASQ47_RS20350_lanthipeptide NZ_PVRP01000003.1 Lanthipeptides: C6355_RS19570_lanthipeptide NZ_SUKE01000008.1 Lanthipeptides: FAZ22_RS20105_lanthipeptide
Lanthipeptides: BASU_RS20300_lanthipeptide
1
D A T I T T T W T V T T T S G F V S S V S N H C
24
D A T I T T T W T V T T T S G F V S S V S N H C
1
D A T I T T T W T V T T T S G F V S S V S N H C
24
95.8% NZ_JALAQD010000016.1 and 1 other:
NZ_JALAUL010000008.1 Lanthipeptides: MOE42_RS14800_lanthipeptide
Lanthipeptides: MOD08_RS18745_lanthipeptide
2
A T I T T T W T V T T T S G F V S S V S N H C
24
A T I T T T W T V T T T S G F V S S V S N H C
1
A T I T T T W T V T T T S G F V S S V S N H C
23
50.0% NZ_FOHE01000012.1 and 1 other:
NZ_FOHE01000012.1 Lanthipeptides: BMX40_RS20540_lanthipeptide
Lanthipeptides: BMX40_RS20545_lanthipeptide
7
T W T V T T T S G F V S S V S N H C
24
T W T V T F+S+V S N H C
4
T W T V T I---F L S T V S N H C
18
with 10 more sequence matches
37.9% NZ_CP089924.1: Lanthipeptides: LVB77_RS02735_lanthipeptide
9
T V T T T S G F V S S V S N H C
24
+V T S G S S V S N H C
5
S V N C T S G--S S V S N H C
18
37.5% NC_014551.1 and 13 others:
NC_017188.1 Lanthipeptides: BAMTA208_RS20935_lanthipeptide NZ_CP026010.1 Lanthipeptides: BS11774_RS22080_lanthipeptide NZ_CP032146.1 Lanthipeptides: D2M30_RS20760_lanthipeptide NZ_CP054584.1 Lanthipeptides: HT135_RS21830_lanthipeptide NZ_CP061284.1 Lanthipeptides: ICJ61_RS04855_lanthipeptide NZ_CP062984.1 Lanthipeptides: IL989_RS03875_lanthipeptide NZ_CP065858.1 Lanthipeptides: I7X10_RS19015_lanthipeptide NZ_DF836084.1 Lanthipeptides: CMW1_RS20390_lanthipeptide NZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21915_lanthipeptide NZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19785_lanthipeptide NZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18870_lanthipeptide NZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19495_lanthipeptide NZ_KQ236689.1 Lanthipeptides: AD710_RS19845_lanthipeptide
Lanthipeptides: BAMF_RS41660_lanthipeptide
5
T T T W T V T T T S G F V S S V S N H C
24
T W T+ T T S++S N C
1
T A G W T L L T T L I G T S T I S N S C
20
34.5% NZ_CP025227.1: Lanthipeptides: allorf_0999194_0999376_lanthipeptide
8
W T V T T T S--G F V S S V S N H C
24
W T V T T S G V ++S C
2
W T V P T T S T I G L V C T L S W E C
20
33.3% NZ_WEHU01000003.1 and 3 others:
NZ_WEHU01000003.1 Lanthipeptides: F9279_RS25000_lanthipeptide NZ_WEHU01000003.1 Lanthipeptides: F9279_RS24995_lanthipeptide NZ_WEHU01000003.1 Lanthipeptides: F9279_RS24990_lanthipeptide
Lanthipeptides: F9279_RS25005_lanthipeptide
16
F V S S V S N H C
24
F V S S+S N H C
15
F V S S I S N H C
23
25.0% NZ_BBYG01000022.1: Lanthipeptides: AOQ37_RS40945_lanthipeptide
18
S S V S N H C
24
S S+S N H C
4
S S I S N H C
10
25.0% NZ_CP014806.1 and 3 others:
NZ_CP014806.1 Lanthipeptides: ATY39_RS17975_lanthipeptide NZ_CP014806.1 Lanthipeptides: ATY39_RS17970_lanthipeptide NZ_CP014806.1 Lanthipeptides: ATY39_RS17965_lanthipeptide
Lanthipeptides: ATY39_RS17980_lanthipeptide
16
F V S S V S N H C
24
F S++S N H C
15
F A S T I S N H C
23
25.0% NZ_CP018933.1 and 24 others:
NZ_CP018933.1 Lanthipeptides: BEI49_RS29525_lanthipeptide NZ_CP018933.1 Lanthipeptides: BEI49_RS29520_lanthipeptide NZ_CP018933.1 Lanthipeptides: BEI49_RS29515_lanthipeptide NZ_CP018933.1 Lanthipeptides: BEI49_RS29510_lanthipeptide NZ_CP018933.1 Lanthipeptides: BEI49_RS29505_lanthipeptide NZ_CP018933.1 Lanthipeptides: BEI49_RS29500_lanthipeptide NZ_CP018933.1 Lanthipeptides: BEI49_RS29495_lanthipeptide NZ_CP018933.1 Lanthipeptides: BEI49_RS29490_lanthipeptide NZ_CP018933.1 Lanthipeptides: BEI49_RS29485_lanthipeptide NZ_CP018933.1 Lanthipeptides: BEI49_RS29480_lanthipeptide NZ_CP018933.1 Lanthipeptides: BEI49_RS29475_lanthipeptide NZ_CP085399.1 Lanthipeptides: LIS81_RS04325_lanthipeptide NZ_CP085399.1 Lanthipeptides: LIS81_RS04320_lanthipeptide NZ_CP085399.1 Lanthipeptides: LIS81_RS04315_lanthipeptide NZ_CP085399.1 Lanthipeptides: LIS81_RS04310_lanthipeptide NZ_CP085399.1 Lanthipeptides: LIS81_RS04305_lanthipeptide NZ_CP085399.1 Lanthipeptides: LIS81_RS04300_lanthipeptide NZ_CP085399.1 Lanthipeptides: LIS81_RS04295_lanthipeptide NZ_CP085399.1 Lanthipeptides: LIS81_RS04290_lanthipeptide NZ_CP085399.1 Lanthipeptides: LIS81_RS04285_lanthipeptide NZ_CP085399.1 Lanthipeptides: LIS81_RS04280_lanthipeptide NZ_NTVN01000027.1 Lanthipeptides: CN415_RS28750_lanthipeptide NZ_PJCU01000036.1 Lanthipeptides: CWE34_RS31080_lanthipeptide NZ_PJCU01000036.1 Lanthipeptides: CWE34_RS31075_lanthipeptide
Lanthipeptides: BEI49_RS29530_lanthipeptide
16
F V S S V S N H C
24
+ S+V S N H C
14
W A S T V S N H C
22
25.0% NZ_GL545262.1: Lanthipeptides: HMPREF0798_RS11830_lanthipeptide
9
T V T T T S G F V S S V S N H C
24
+++T F+S++S H C
1
S I S T L V A F L S T I S Q H C
16
21.2% NZ_MIFN01000022.1 and 1 other:
NZ_MIFN01000022.1 Lanthipeptides: BHL26_RS15355_lanthipeptide
Lanthipeptides: BHL26_RS15355_lanthipeptide
4
I T T T W T V T T T S G F V S S
19
+ T T +T S G++S S
6
L A T T G A L T A V S G Y I S S
21
17.4% NZ_QVFA01000004.1: Lanthipeptides: DW882_RS14120_lanthipeptide
5
T T T W T V T T T S G F V S S V S N
22
T T T ++ S V S++S N
14
T T T A I I S A I S S V V S A I S N
31

#### Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine
Module view
Shows module structures for each candidate cluster in NRPS and PKS regions.
Genes are shown in predicted order, and are only present when containing at least one complete module.
A domain glossary is available here, and an explanation of the visualisation is available here.
Legend
Lanthipeptides
NRPS/PKS products
NRPS/PKS substrates
Core peptide predictions
Lists the possible core peptides in the region. Each core peptide lists the number of lanthionine bridges, possible molecular weights, and the scores for cleavage site prediction and RODEO.

ctg1_193 - Class III
  Cleavage pHMM score: 3.30
  RODEO score: 0
  Monoisotopic mass: 2299.2 Da
  Molecular weight: 2300.5 Da
  Number of bridges: 1
  Alternative weights
    (assuming N unmodified Ser/Thr residues)
    2318.5 Da N = 1
    2336.5 Da N = 2
    2354.5 Da N = 3
    2372.5 Da N = 4
    2390.6 Da N = 5
    2408.6 Da N = 6
    2426.6 Da N = 7
    2444.6 Da N = 8
    2462.6 Da N = 9
    2480.7 Da N = 10
    2498.7 Da N = 11

Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 1, location 193326 - 277134:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (Val - D-Phe) + (Asp - D-Asn - Gly) + (ccmal) + (ccmal) + (mal - D-Glu)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 199448 - 277134:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (Val - D-Phe) + (Asp - D-Asn - Gly) + (ccmal) + (ccmal) + (mal - D-Glu)

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS substrate predictions
Shows the predicted substrates for each adenylation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).

ctg1_208: Val - Phe
  Search NORINE for peptide: strict or relaxed
  AMP-binding (233..630): Val
  nrpys: Val
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
    Large clusters prediction:
      Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
    Small clusters prediction:
      Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
    Single AA prediction:
      val (Val)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAFWLGGTFK
    Nearest Stachelhaus code(s):
      Val DAFWLGGTFK (94% 8Å match)
    Stachelhaus code match:
      100% (strong)
  AMP-binding (1272..1663): Phe
  nrpys: Phe
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
    Large clusters prediction:
      Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
    Small clusters prediction:
      Unpolar aromatic ring (Phe, Trp)
    Single AA prediction:
      phe (Phe)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAWTIAAICK
    Nearest Stachelhaus code(s):
      Phe DAWTIAAICK (91% 8Å match)
    Stachelhaus code match:
      100% (strong)
ctg1_209: Asp - Asn - Gly
  Search NORINE for peptide: strict or relaxed
  AMP-binding (264..665): Asp
  nrpys: Asp
  SVM prediction details:
    Predicted physicochemical class:
      hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
    Large clusters prediction:
      Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
    Small clusters prediction:
      asp,asn (Asp, Asn)
    Single AA prediction:
      asp (Asp)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DLTKIGHVGK
    Nearest Stachelhaus code(s):
      Asp DLTKIGHVGK (65% 8Å match)
    Stachelhaus code match:
      100% (strong)
  AMP-binding (1303..1697): Asn
  nrpys: Asn
  SVM prediction details:
    Predicted physicochemical class:
      hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
    Large clusters prediction:
      Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
    Small clusters prediction:
      asp,asn (Asp, Asn)
    Single AA prediction:
      asn (Asn)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DLTKIGEVGK
    Nearest Stachelhaus code(s):
      Asn DLTKIGEVGK (94% 8Å match)
    Stachelhaus code match:
      100% (strong)
  AMP-binding (2805..3198): Gly
  nrpys: Gly
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
    Large clusters prediction:
      Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
    Small clusters prediction:
      Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
    Single AA prediction:
      gly (Gly)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DILQMGMIWK
    Nearest Stachelhaus code(s):
      Gly DILQMGMVWK (85% 8Å match)
    Stachelhaus code match:
      90% (moderate)
ctg1_210: mal
  PKS_AT (6..293): mal
  ATSignature: Malonyl-CoA
  Top 3 matches:
    Malonyl-CoA: 83.3%
    inactive: 75.0%
    2-Methylbutyryl-CoA: 54.2%
  minowa: Malonyl-CoA
  Prediction, score:
    Malonyl-CoA: 111.1
    inactive: 50.8
    Methoxymalonyl-CoA: 39.3
    Isobutyryl-CoA: 31.6
    Methylmalonyl-CoA: 29.7
    Propionyl-CoA: 12.0
    CHC-CoA: 10.8
    fatty_acid: 10.4
    Benzoyl-CoA: 10.0
    trans-1,2-CPDA: 0.0
    Ethylmalonyl-CoA: 0.0
    Acetyl-CoA: 0.0
    3-Methylbutyryl-CoA: 0.0
    2-Methylbutyryl-CoA: 0.0
ctg1_214: Glu
  Search NORINE for peptide: strict or relaxed
  AMP-binding (1198..1599): Glu
  nrpys: Glu
  SVM prediction details:
    Predicted physicochemical class:
      hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
    Large clusters prediction:
      Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
    Small clusters prediction:
      glu,gln (Glu, Gln)
    Single AA prediction:
      N/A
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAKDIGVVDK
    Nearest Stachelhaus code(s):
      Glu DAKDIGVVDK (85% 8Å match)
    Stachelhaus code match:
      100% (strong)

gnlProkkaANBMDGDF_1 - Region 2 - NRPS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 345,741 - 411,151 nt. (total: 65,411 nt) Show pHMM detection rules used
NRPS: cds(Condensation and (AMP-binding or A-OX))

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
NRPS/PKS domains
KnownClusterBlast
NRPS/PKS modules
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A domain glossary is available here, and an explanation of the visualisation is available here.
Selected features only
Show module domains
Similar known gene clusters from MIBiG 3.1
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.
Module view
Shows module structures for each candidate cluster in NRPS and PKS regions.
Genes are shown in predicted order, and are only present when containing at least one complete module.
A domain glossary is available here, and an explanation of the visualisation is available here.
Legend
NRPS/PKS products
NRPS/PKS substrates
Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 4, location 345740 - 411151:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (Glu - Leu - D-Leu) + (Val - Asp - D-Leu) + (Ile)

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS substrate predictions
Shows the predicted substrates for each adenylation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).

ctg1_323: Glu - Leu - Leu
  Search NORINE for peptide: strict or relaxed
  AMP-binding (465..867): Glu
  nrpys: Glu
  SVM prediction details:
    Predicted physicochemical class:
      hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
    Large clusters prediction:
      Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
    Small clusters prediction:
      glu,gln (Glu, Gln)
    Single AA prediction:
      gln (Gln)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAKDLGVVDK
    Nearest Stachelhaus code(s):
      Glu DAKDLGVVDK (100% 8Å match)
    Stachelhaus code match:
      100% (strong)
  AMP-binding (1511..1908): Leu
  nrpys: Leu
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
    Large clusters prediction:
      Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
    Small clusters prediction:
      Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
    Single AA prediction:
      leu (Leu)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAFMMGMVFK
    Nearest Stachelhaus code(s):
      Leu DAFMMGMVFK (100% 8Å match)
    Stachelhaus code match:
      100% (strong)
  AMP-binding (2550..2939): Leu
  nrpys: Leu
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
    Large clusters prediction:
      Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
    Small clusters prediction:
      Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
    Single AA prediction:
      leu (Leu)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAWFLGNVVK
    Nearest Stachelhaus code(s):
      Leu DAWFLGNVVK (97% 8Å match)
    Stachelhaus code match:
      100% (strong)
ctg1_324: Val - Asp - Leu
  Search NORINE for peptide: strict or relaxed
  AMP-binding (470..864): Val
  nrpys: Val
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
    Large clusters prediction:
      Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
    Small clusters prediction:
      Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
    Single AA prediction:
      val (Val)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAFWIGGTFK
    Nearest Stachelhaus code(s):
      Val DAFWIGGTFK (100% 8Å match)
    Stachelhaus code match:
      100% (strong)
  AMP-binding (1502..1904): Asp
  nrpys: Asp
  SVM prediction details:
    Predicted physicochemical class:
      hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
    Large clusters prediction:
      Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
    Small clusters prediction:
      asp,asn (Asp, Asn)
    Single AA prediction:
      asp (Asp)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DLTKVGHIGK
    Nearest Stachelhaus code(s):
      Asp DLTKVGHIGK (100% 8Å match)
    Stachelhaus code match:
      100% (strong)
  AMP-binding (2547..2936): Leu
  nrpys: Leu
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
    Large clusters prediction:
      Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
    Small clusters prediction:
      Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
    Single AA prediction:
      leu (Leu)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAWFLGNVVK
    Nearest Stachelhaus code(s):
      Leu DAWFLGNVVK (97% 8Å match)
    Stachelhaus code match:
      100% (strong)
ctg1_325: Ile
  Search NORINE for peptide: strict or relaxed
  AMP-binding (469..867): Ile
  nrpys: Ile
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
    Large clusters prediction:
      Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
    Small clusters prediction:
      Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
    Single AA prediction:
      ile (Ile)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DGFFLGVVFK
    Nearest Stachelhaus code(s):
      Ile DGFFLGVVFK (88% 8Å match)
    Stachelhaus code match:
      100% (strong)

gnlProkkaANBMDGDF_1 - Region 3 - PKS-like
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 924,760 - 966,004 nt. (total: 41,245 nt) Show pHMM detection rules used
PKS-like: (t2pks2 or ksIII)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
NRPS/PKS domains
KnownClusterBlast
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A domain glossary is available here, and an explanation of the visualisation is available here.
Selected features only
Show module domains
Similar known gene clusters from MIBiG 3.1
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.
NRPS/PKS substrates
NRPS/PKS substrate predictions
Shows the predicted substrates for each adenylation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).

gnlProkkaANBMDGDF_1 - Region 4 - terpene
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 1,050,778 - 1,071,521 nt. (total: 20,744 nt) Show pHMM detection rules used
terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
KnownClusterBlast
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Similar known gene clusters from MIBiG 3.1
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.

No matches found.

gnlProkkaANBMDGDF_1 - Region 5 - NRPS,T3PKS,transAT-PKS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 1,624,692 - 1,734,781 nt. (total: 110,090 nt) Show pHMM detection rules used
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
NRPS: cds(Condensation and (AMP-binding or A-OX))

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
NRPS/PKS domains
KnownClusterBlast
NRPS/PKS modules
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A domain glossary is available here, and an explanation of the visualisation is available here.
Selected features only
Show module domains
Similar known gene clusters from MIBiG 3.1
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.
Module view
Shows module structures for each candidate cluster in NRPS and PKS regions.
Genes are shown in predicted order, and are only present when containing at least one complete module.
A domain glossary is available here, and an explanation of the visualisation is available here.
Legend
NRPS/PKS products
NRPS/PKS substrates
Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 7, location 1624691 - 1734781:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (Gly - ccmal - ohmal - mal) + (ccmal - mal - ohmal - mal) + (Me-ccmal - ohmal) + (Ala - ccmal - ccmal - Me-ccmal) + (mal - mal)

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS substrate predictions
Shows the predicted substrates for each adenylation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).

ctg1_1593: mal
  PKS_AT (4..285): mal
  ATSignature: Malonyl-CoA
  Top 3 matches:
    Malonyl-CoA: 87.5%
    inactive: 62.5%
  minowa: Malonyl-CoA
  Prediction, score:
    Malonyl-CoA: 98.2
    inactive: 43.3
    Methoxymalonyl-CoA: 36.6
    Methylmalonyl-CoA: 24.6
    Propionyl-CoA: 24.5
    2-Methylbutyryl-CoA: 5.7
    trans-1,2-CPDA: 0.0
    fatty_acid: 0.0
    Isobutyryl-CoA: 0.0
    Ethylmalonyl-CoA: 0.0
    CHC-CoA: 0.0
    Benzoyl-CoA: 0.0
    Acetyl-CoA: 0.0
    3-Methylbutyryl-CoA: 0.0
ctg1_1594: mal
  PKS_AT (7..293): mal
  ATSignature: (unknown)
  No matches above 50%
  minowa: Malonyl-CoA
  Prediction, score:
    Malonyl-CoA: 29.5
    inactive: 18.8
    Methoxymalonyl-CoA: 14.7
    Benzoyl-CoA: 9.3
    Isobutyryl-CoA: 8.6
    trans-1,2-CPDA: 0.0
    fatty_acid: 0.0
    Propionyl-CoA: 0.0
    Methylmalonyl-CoA: 0.0
    Ethylmalonyl-CoA: 0.0
    CHC-CoA: 0.0
    Acetyl-CoA: 0.0
    3-Methylbutyryl-CoA: 0.0
    2-Methylbutyryl-CoA: 0.0
ctg1_1595: mal
  PKS_AT (4..282): mal
  ATSignature: Malonyl-CoA
  Top 3 matches:
    Malonyl-CoA: 91.7%
    inactive: 66.7%
  minowa: Malonyl-CoA
  Prediction, score:
    Malonyl-CoA: 96.9
    inactive: 30.8
    Methoxymalonyl-CoA: 27.7
    Methylmalonyl-CoA: 15.2
    trans-1,2-CPDA: 0.0
    fatty_acid: 0.0
    Propionyl-CoA: 0.0
    Isobutyryl-CoA: 0.0
    Ethylmalonyl-CoA: 0.0
    CHC-CoA: 0.0
    Benzoyl-CoA: 0.0
    Acetyl-CoA: 0.0
    3-Methylbutyryl-CoA: 0.0
    2-Methylbutyryl-CoA: 0.0
ctg1_1600: pk - Gly
  Search NORINE for peptide: strict or relaxed
  CAL_domain (74..476): pk
  minowa: fatty_acid
  Prediction, score:
    fatty_acid: 22.2
    shikimic_acid: 0.0
    NH2: 0.0
    Acetyl-CoA: 0.0
    AHBA: 0.0
  AMP-binding (1145..1540): Gly
  nrpys: Gly
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
    Large clusters prediction:
      Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
    Small clusters prediction:
      Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
    Single AA prediction:
      gly (Gly)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DILQLGMIWK
    Nearest Stachelhaus code(s):
      Gly DILQLGMIWK (100% 8Å match)
    Stachelhaus code match:
      100% (strong)
ctg1_1603: Ala
  Search NORINE for peptide: strict or relaxed
  AMP-binding (492..896): Ala
  nrpys: Ala
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
    Large clusters prediction:
      N/A
    Small clusters prediction:
      N/A
    Single AA prediction:
      N/A
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DVSNMAIIYK
    Nearest Stachelhaus code(s):
      Ala DVSNMAIIYK (88% 8Å match)
    Stachelhaus code match:
      100% (strong)

gnlProkkaANBMDGDF_1 - Region 6 - NRPS,betalactone,transAT-PKS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 1,795,152 - 1,880,708 nt. (total: 85,557 nt) Show pHMM detection rules used
NRPS: cds(Condensation and (AMP-binding or A-OX))
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
betalactone: (HMGL-like and AMP-binding)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
NRPS/PKS domains
KnownClusterBlast
NRPS/PKS modules
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A domain glossary is available here, and an explanation of the visualisation is available here.
Selected features only
Show module domains
Similar known gene clusters from MIBiG 3.1
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.
Module view
Shows module structures for each candidate cluster in NRPS and PKS regions.
Genes are shown in predicted order, and are only present when containing at least one complete module.
A domain glossary is available here, and an explanation of the visualisation is available here.
Legend
NRPS/PKS products
NRPS/PKS substrates
Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 8, location 1795151 - 1880708:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (mal - Asn) + (D-Tyr - D-Asn - Pro - Glu) + (D-Ser - Thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 9, location 1795151 - 1872408:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (mal - Asn) + (D-Tyr - D-Asn - Pro - Glu) + (D-Ser - Thr)

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS substrate predictions
Shows the predicted substrates for each adenylation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).

ctg1_1720: Ser - Thr
  Search NORINE for peptide: strict or relaxed
  AMP-binding (267..675): Ser
  nrpys: Ser
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
    Large clusters prediction:
      Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
    Small clusters prediction:
      ser (Ser)
    Single AA prediction:
      ser (Ser)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DVWHFSLIDK
    Nearest Stachelhaus code(s):
      Ser DVWHFSLIDK (100% 8Å match)
    Stachelhaus code match:
      100% (strong)
  AMP-binding (1790..2189): Thr
  nrpys: Thr
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
    Large clusters prediction:
      Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
    Small clusters prediction:
      thr (Thr)
    Single AA prediction:
      thr (Thr)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DFWNIGMVHK
    Nearest Stachelhaus code(s):
      Thr DFWNIGMVHK (100% 8Å match)
    Stachelhaus code match:
      100% (strong)
ctg1_1721: Tyr - Asn - Pro - Glu
  Search NORINE for peptide: strict or relaxed
  AMP-binding (268..662): Tyr
  nrpys: Tyr
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
    Large clusters prediction:
      Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
    Small clusters prediction:
      Polar aromatic ring (Tyr, bOH-Tyr)
    Single AA prediction:
      tyr (Tyr)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DALSVGEVVK
    Nearest Stachelhaus code(s):
      Tyr DALSVGEVVK (100% 8Å match)
    Stachelhaus code match:
      100% (strong)
  AMP-binding (1778..2173): Asn
  nrpys: Asn
  SVM prediction details:
    Predicted physicochemical class:
      hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
    Large clusters prediction:
      Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
    Small clusters prediction:
      asp,asn (Asp, Asn)
    Single AA prediction:
      asn (Asn)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DLTKIGEVGK
    Nearest Stachelhaus code(s):
      Asn DLTKIGEVGK (100% 8Å match)
    Stachelhaus code match:
      100% (strong)
  AMP-binding (3286..3683): Pro
  nrpys: Pro
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
    Large clusters prediction:
      Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
    Small clusters prediction:
      pro (Pro)
    Single AA prediction:
      pro (Pro)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DVQFIAHVVK
    Nearest Stachelhaus code(s):
      Pro DVQFIAHVVK (100% 8Å match)
    Stachelhaus code match:
      100% (strong)
  AMP-binding (4325..4732): Glu
  nrpys: Glu
  SVM prediction details:
    Predicted physicochemical class:
      hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
    Large clusters prediction:
      Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
    Small clusters prediction:
      glu,gln (Glu, Gln)
    Single AA prediction:
      gln (Gln)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAKDLGVVDK
    Nearest Stachelhaus code(s):
      Glu DAKDLGVVDK (100% 8Å match)
    Stachelhaus code match:
      100% (strong)
ctg1_1722: pk - Asn
  Search NORINE for peptide: strict or relaxed
  CAL_domain (36..478): pk
  minowa: fatty_acid
  Prediction, score:
    fatty_acid: 618.7
    NH2: 18.8
    shikimic_acid: 0.0
    Acetyl-CoA: 0.0
    AHBA: 0.0
  AMP-binding (2956..3351): Asn
  nrpys: Asn
  SVM prediction details:
    Predicted physicochemical class:
      hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
    Large clusters prediction:
      Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
    Small clusters prediction:
      asp,asn (Asp, Asn)
    Single AA prediction:
      asn (Asn)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DLTKIGEVGK
    Nearest Stachelhaus code(s):
      Asn DLTKIGEVGK (100% 8Å match)
    Stachelhaus code match:
      100% (strong)
ctg1_1723: mal
  PKS_AT (6..291): mal
  ATSignature: Malonyl-CoA
  Top 3 matches:
    Malonyl-CoA: 62.5%
    inactive: 54.2%
  minowa: Malonyl-CoA
  Prediction, score:
    Malonyl-CoA: 77.2
    inactive: 33.6
    Methoxymalonyl-CoA: 21.2
    Propionyl-CoA: 13.0
    Methylmalonyl-CoA: 11.7
    trans-1,2-CPDA: 0.0
    fatty_acid: 0.0
    Isobutyryl-CoA: 0.0
    Ethylmalonyl-CoA: 0.0
    CHC-CoA: 0.0
    Benzoyl-CoA: 0.0
    Acetyl-CoA: 0.0
    3-Methylbutyryl-CoA: 0.0
    2-Methylbutyryl-CoA: 0.0
ctg1_1741: X
  AMP-binding (12..445): X
  nrpys: Asp, Asn, Glu, Gln, Aad
  SVM prediction details:
    Predicted physicochemical class:
      N/A
    Large clusters prediction:
      Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
    Small clusters prediction:
      N/A
    Single AA prediction:
      N/A
  Stachelhaus prediction details:
    Stachelhaus sequence:
      -GLHIAAPVK
    Nearest Stachelhaus code(s):
      Z-ahmohOrn FGLHIASTCK (56% 8Å match)
      Ile DGLFIGIPVK (32% 8Å match)
    Stachelhaus code match:
      60% (weak)

gnlProkkaANBMDGDF_1 - Region 7 - terpene
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 1,924,757 - 1,946,640 nt. (total: 21,884 nt) Show pHMM detection rules used
terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
KnownClusterBlast
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Similar known gene clusters from MIBiG 3.1
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.

No matches found.

gnlProkkaANBMDGDF_1 - Region 8 - T3PKS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 2,001,008 - 2,042,108 nt. (total: 41,101 nt) Show pHMM detection rules used
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
KnownClusterBlast
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Similar known gene clusters from MIBiG 3.1
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.

No matches found.

NRPS/PKS substrates
NRPS/PKS substrate predictions
Shows the predicted substrates for each adenylation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).

gnlProkkaANBMDGDF_1 - Region 9 - NRP-metallophore,NRPS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 2,801,341 - 2,853,073 nt. (total: 51,733 nt) Show pHMM detection rules used
NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX))

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
NRPS/PKS domains
KnownClusterBlast
NRPS/PKS modules
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A domain glossary is available here, and an explanation of the visualisation is available here.
Selected features only
Show module domains
Similar known gene clusters from MIBiG 3.1
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.
Module view
Shows module structures for each candidate cluster in NRPS and PKS regions.
Genes are shown in predicted order, and are only present when containing at least one complete module.
A domain glossary is available here, and an explanation of the visualisation is available here.
Legend
NRPS/PKS products
NRPS/PKS substrates
Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 13, location 2801340 - 2853073:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (Gly - Thr)

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS substrate predictions
Shows the predicted substrates for each adenylation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).

ctg1_2749: Gly - Thr
  Search NORINE for peptide: strict or relaxed
  AMP-binding (456..855): Gly
  nrpys: Gly
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
    Large clusters prediction:
      Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
    Small clusters prediction:
      Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
    Single AA prediction:
      gly (Gly)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DILQLGLIWK
    Nearest Stachelhaus code(s):
      Gly DILQLGLIWK (100% 8Å match)
    Stachelhaus code match:
      100% (strong)
  AMP-binding (1513..1929): Thr
  nrpys: Thr
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
    Large clusters prediction:
      Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
    Small clusters prediction:
      thr (Thr)
    Single AA prediction:
      thr (Thr)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DFWNIGMVHK
    Nearest Stachelhaus code(s):
      Thr DFWNIGMVHK (100% 8Å match)
    Stachelhaus code match:
      100% (strong)
ctg1_2751: diOH-Bz
  Search NORINE for peptide: strict or relaxed
  AMP-binding (31..435): diOH-Bz
  nrpys: diOH-Bz
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
    Large clusters prediction:
      Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
    Small clusters prediction:
      Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
    Single AA prediction:
      dhb (diOH-Bz)
  Stachelhaus prediction details:
    Stachelhaus sequence:
      PLPAQGVVNK
    Nearest Stachelhaus code(s):
      diOH-Bz PLPAQGVVNK (97% 8Å match)
      diOH-Bz or Sal PLPAQGVVNK (94% 8Å match)
    Stachelhaus code match:
      100% (strong)

gnlProkkaANBMDGDF_1 - Region 10 - other
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 3,358,364 - 3,399,782 nt. (total: 41,419 nt) Show pHMM detection rules used
other: (LmbU or Neocarzinostat or bcpB or frbD or mitE or vlmB or prnB or CaiA or bacilysin or orf2_PTase)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
KnownClusterBlast
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Similar known gene clusters from MIBiG 3.1
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.