GCA_002076025.1 - 6 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaCMDGLOJP_9 (original name was: gnl|Prokka|CMDGLOJP_9)

Region	Type	From	To	Most similar known cluster		Similarity
Region 9.1	RiPP-like	25,131	36,270
Region 9.2	RiPP-like	156,239	166,631

gnlProkkaCMDGLOJP_20 (original name was: gnl|Prokka|CMDGLOJP_20)

Region	Type	From	To	Most similar known cluster		Similarity
Region 20.1	lanthipeptide-class-v	7,212	49,101	N-octanoyl-Met-Phe-H	NRP	22%
Region 20.2	NRPS-like	172,182	215,193	nosiheptide	RiPP:Thiopeptide	7%

gnlProkkaCMDGLOJP_23 (original name was: gnl|Prokka|CMDGLOJP_23)

Region	Type	From	To	Most similar known cluster		Similarity
Region 23.1	RiPP-like,lanthipeptide-class-ii	3,914	36,613
Region 23.2	lanthipeptide-class-iii,RRE-containing	70,633	100,289

Region	Type	From	To	Most similar known cluster	Similarity
Region 9.1	RiPP-like	25,131	36,270
Region 9.2	RiPP-like	156,239	166,631
Region 20.1	lanthipeptide-class-v	7,212	49,101	N-octanoyl-Met-Phe-H	NRP	22%
Region 20.2	NRPS-like	172,182	215,193	nosiheptide	RiPP:Thiopeptide	7%
Region 23.1	RiPP-like,lanthipeptide-class-ii	3,914	36,613
Region 23.2	lanthipeptide-class-iii,RRE-containing	70,633	100,289

No secondary metabolite regions were found in these records:: gnlProkkaCMDGLOJP_1 (original name was: gnl|Prokka|CMDGLOJP_1); gnlProkkaCMDGLOJP_2 (original name was: gnl|Prokka|CMDGLOJP_2); gnlProkkaCMDGLOJP_3 (original name was: gnl|Prokka|CMDGLOJP_3); gnlProkkaCMDGLOJP_4 (original name was: gnl|Prokka|CMDGLOJP_4); gnlProkkaCMDGLOJP_5 (original name was: gnl|Prokka|CMDGLOJP_5); gnlProkkaCMDGLOJP_6 (original name was: gnl|Prokka|CMDGLOJP_6); gnlProkkaCMDGLOJP_7 (original name was: gnl|Prokka|CMDGLOJP_7); gnlProkkaCMDGLOJP_8 (original name was: gnl|Prokka|CMDGLOJP_8); gnlProkkaCMDGLOJP_10 (original name was: gnl|Prokka|CMDGLOJP_10); gnlProkkaCMDGLOJP_11 (original name was: gnl|Prokka|CMDGLOJP_11); gnlProkkaCMDGLOJP_12 (original name was: gnl|Prokka|CMDGLOJP_12); gnlProkkaCMDGLOJP_13 (original name was: gnl|Prokka|CMDGLOJP_13); gnlProkkaCMDGLOJP_14 (original name was: gnl|Prokka|CMDGLOJP_14); gnlProkkaCMDGLOJP_15 (original name was: gnl|Prokka|CMDGLOJP_15); gnlProkkaCMDGLOJP_16 (original name was: gnl|Prokka|CMDGLOJP_16); gnlProkkaCMDGLOJP_17 (original name was: gnl|Prokka|CMDGLOJP_17); gnlProkkaCMDGLOJP_18 (original name was: gnl|Prokka|CMDGLOJP_18); gnlProkkaCMDGLOJP_19 (original name was: gnl|Prokka|CMDGLOJP_19); gnlProkkaCMDGLOJP_21 (original name was: gnl|Prokka|CMDGLOJP_21); gnlProkkaCMDGLOJP_22 (original name was: gnl|Prokka|CMDGLOJP_22); gnlProkkaCMDGLOJP_24 (original name was: gnl|Prokka|CMDGLOJP_24); gnlProkkaCMDGLOJP_25 (original name was: gnl|Prokka|CMDGLOJP_25); gnlProkkaCMDGLOJP_26 (original name was: gnl|Prokka|CMDGLOJP_26)

Compact view

gnlProkkaCMDGLOJP_9 - Region 1 - RiPP-like

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Location: 25,131 - 36,270 nt. (total: 11,140 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

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core biosynthetic genes

additional biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaCMDGLOJP_9 - Region 2 - RiPP-like

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Location: 156,239 - 166,631 nt. (total: 10,393 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

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core biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaCMDGLOJP_20 - Region 1 - lanthipeptide-class-v

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Location: 7,212 - 49,101 nt. (total: 41,890 nt) Show pHMM detection rules used

lanthipeptide-class-v: (APH and HopA1)

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core biosynthetic genes

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KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Lanthipeptides

Core peptide predictions

No core peptides found.

gnlProkkaCMDGLOJP_20 - Region 2 - NRPS-like

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Location: 172,182 - 215,193 nt. (total: 43,012 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 2, location 172181 - 215193:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg20_155

AMP-binding (14..381): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DFFTIGIIYK
Nearest Stachelhaus code(s):: Ile DFWNIGIIYK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

gnlProkkaCMDGLOJP_23 - Region 1 - RiPP-like,lanthipeptide-class-ii

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Location: 3,914 - 36,613 nt. (total: 32,700 nt) Show pHMM detection rules used

Region on contig edge.

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))
lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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KnownClusterBlast

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg23_25

ctg23_16

Leader:

MVTLKNDNVVGDRGDGAAAA

Core:

VGSARELVDGLVSSGRWIPCSSG

Core with dehydrations:

VGDhaARELVDGLVDhaDhaGRWIPCysDhaDhaG

antiSMASH-DB 4.0 matches for ctg23_16

100.0% NZ_CABWKH010000001.1and 1 other:

NZ_CP010411.1 Lanthipeptides: RY67_RS05310_lanthipeptide

Lanthipeptides: BIFLH14_RS13125_lanthipeptide

1

VGSARELVDGLVSSGRWIPCSSG

23

VGSARELVDGLVSSGRWIPCSSG

1

VGSARELVDGLVSSGRWIPCSSG

23

18.8% NZ_CP031746.1: Lanthipeptides: allorf_1813979_1814164_lanthipeptide

11

LVSSGRWIPCS

21

+V RW+PCS

4

VVGCARWVPCS

14

allorf_013579_013818

Leader:

MHTSFSSLKDHRLTYFRTTWHHFRAISGLQGA

Core:

DLRLSGHLPPFQGALHPRKRKYKRLCFNAIKPFWGRVYFGNSPLKRR

Core with dehydrations:

DLRLDhaGHLPPFQGALHPRKRKYKRLCysFNAIKPFWGRVYFGNDhaPLKRR

antiSMASH-DB 4.0 matches for allorf_013579_013818

19.1% NZ_CP017316.1: Lanthipeptides: allorf_6268660_6268842_lanthipeptide

3

RLSGHLPPFQGALHPRKRKYKRL

25

R +GH P QG H R+ + R

7

RGAGHAP-CQGVCHQREERADRA

28

17.0% NZ_CAJHJS010000009.1: Lanthipeptides: allorf_72861_73019_lanthipeptide

17

PRKRKYKRLCFNAIKPFWGRVYFGN

41

+ K++ C N IK V F N

1

DKTAKHRNFCVNLIKSELFLVTFSN

25

17.0% NZ_CP069396.1: Lanthipeptides: allorf_00880550_00880726_lanthipeptide

25

LCFNAIKPFWGRVYFGNS

42

+CFN+ + GR + GNS

9

VCFNSHRAGNGRAHEGNS

26

with 2 more sequence matches

14.9% NZ_KV815277.1: Lanthipeptides: allorf_240790_240948_lanthipeptide

26

CFNAIKPFWGRVYFGNSPLK

45

C N++ WG N LK

11

CLNSVIKIWGDTINFNLSLK

30

12.8% NZ_JAHXMW010000012.1and 1 other:

NZ_JAHXMW010000012.1 Lanthipeptides: allorf_053850_054050_lanthipeptide

Lanthipeptides: allorf_053850_054050_lanthipeptide

34

WGRVYFGNSPLK

45

W + Y GNS L

6

WNKAYVGNSLLN

17

allorf_023285_023395

Leader:

MQEQTKVPHS

Core:

CVRVQTHPLLAMYGLTILVNSLSICF

Core with dehydrations:

CysVRVQDhbHPLLAMYGLDhbILVNDhaLDhaICysF

allorf_026704_026814

Leader:

MHSVMLPMLFNLHIHGG

Core:

GVINETWILSGCIVDGVWS

Core with dehydrations:

GVINEDhbWILDhaGCysIVDGVWDha

antiSMASH-DB 4.0 matches for allorf_026704_026814

100.0% NZ_CP010411.1: Lanthipeptides: allorf_1269156_1269266_lanthipeptide

1

GVINETWILSGCIVDGVWS

19

GVINETWILSGCIVDGVWS

1

GVINETWILSGCIVDGVWS

19

allorf_027582_027722

Leader:

MRHYDAVAGS

Core:

RLPDAICRLSFIGCRLSDASVSAQRCDSVPVRASIV

Core with dehydrations:

RLPDAICysRLDhaFIGCysRLDhaDADhaVDhaAQRCysDDhaVPVRADhaIV

antiSMASH-DB 4.0 matches for allorf_027582_027722

100.0% NZ_CP010411.1: Lanthipeptides: allorf_1270034_1270174_lanthipeptide

1

RLPDAICRLSFIGCRLSDASVSAQRCDSVPVRASIV

36

RLPDAICRLSFIGCRLSDASVSAQRCDSVPVRASIV

1

RLPDAICRLSFIGCRLSDASVSAQRCDSVPVRASIV

36

23.7% NZ_CP066762.1: Lanthipeptides: allorf_2733265_2733486_lanthipeptide

2

LPDAICRLSFIGCRLSDASVSAQRCDSVPVRA

33

LP A R+ G LS + AQR S P RA

21

LPAARQRIECHG--LSVKNGRAQRSGSSPARA

50

16.7% NZ_JADOEQ010000001.1: Lanthipeptides: allorf_121270_121383_lanthipeptide

13

GCRLSDASVSAQRC

26

GC+LS +V+ +C

6

GCQLSFFNVALMKC

19

with 1 more sequence matches

15.9% NZ_JAGPNM010000006.1: Lanthipeptides: J3B89_RS14930_lanthipeptide

13

GCRLSDASVSAQRCDSVP

30

GCR+SD + C +P

27

GCRISDPTGMTSPCCQIP

44

allorf_027756_027830

Leader:

MRKRVFFA

Core:

YWDNSRCPGLRVNRST

Core with dehydrations:

YWDNDhaRCysPGLRVNRDhaDhb

antiSMASH-DB 4.0 matches for allorf_027756_027830

100.0% NZ_CP010411.1: Lanthipeptides: allorf_1270208_1270282_lanthipeptide

1

YWDNSRCPGLRVNRST

16

YWDNSRCPGLRVNRST

1

YWDNSRCPGLRVNRST

16

37.5% NZ_KB913037.1: Lanthipeptides: allorf_10327850_10328080_lanthipeptide

4

NSRCPGLRVNR

14

SRCPG R

3

GSRCPGSPAGR

13

31.2% NZ_FQZK01000006.1and 1 other:

NZ_FQZK01000006.1 Lanthipeptides: allorf_207492_207587_lanthipeptide

Lanthipeptides: allorf_207492_207587_lanthipeptide

2

WDNSRCPG

9

W RCPG

1

WSRCRCPG

8

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg23_16 - Class II

Cleavage pHMM score: -6.00

RODEO score: 0

Monoisotopic mass: 2241.2 Da

Molecular weight: 2242.5 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2260.5 Da N = 1; 2278.5 Da N = 2; 2296.6 Da N = 3; 2314.6 Da N = 4

allorf_013579_013818 - Class II

Cleavage pHMM score: -6.80

RODEO score: 0

Monoisotopic mass: 5584.1 Da

Molecular weight: 5587.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 5605.6 Da N = 1

allorf_023285_023395 - Class II

Cleavage pHMM score: -7.20

RODEO score: 0

Monoisotopic mass: 2818.5 Da

Molecular weight: 2820.4 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2838.4 Da N = 1; 2856.5 Da N = 2

allorf_026704_026814 - Class II

Cleavage pHMM score: -4.10

RODEO score: 0

Monoisotopic mass: 1993.0 Da

Molecular weight: 1994.3 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2012.3 Da N = 1; 2030.3 Da N = 2

allorf_027582_027722 - Class II

Cleavage pHMM score: -3.90

RODEO score: 0

Monoisotopic mass: 3752.0 Da

Molecular weight: 3754.3 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3772.4 Da N = 1; 3790.4 Da N = 2; 3808.4 Da N = 3

allorf_027756_027830 - Class II

Cleavage pHMM score: -5.40

RODEO score: 0

Monoisotopic mass: 1868.9 Da

Molecular weight: 1870.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1888.1 Da N = 1; 1906.1 Da N = 2

gnlProkkaCMDGLOJP_23 - Region 2 - RRE-containing,lanthipeptide-class-iii

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Location: 70,633 - 100,289 nt. (total: 29,657 nt) Show pHMM detection rules used

lanthipeptide-class-iii: (micKC and not PKS_KS)
RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

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core biosynthetic genes

additional biosynthetic genes

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regulatory genes

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Gene details

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Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Lanthipeptides

Core peptide predictions

No core peptides found.