LOCUS       c00071_gnlPro..         8163 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PMCCECLB_80.
ACCESSION   c00071_gnlPro..
VERSION     c00071_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-29 05:33:57
            Original ID  :: gnl|Prokka|PMCCECLB_80
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 8163
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..8163
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3889:7020]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3890..7020
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..8163
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..8163
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..110
                     /locus_tag="ctg71_1"
                     /transl_table=11
                     /translation="IQGLISIKNILKVFIILIVDLYLITCIIKGGLMLM"
     CDS             127..1356
                     /locus_tag="ctg71_2"
                     /transl_table=11
                     /translation="MSKNNILKLNVANDLGYGSVKAKVEDTNIHFPSVIAIQREQDLNK
                     PVEFNSNQEKLTYLEGMINHMDVTISSSAVKTQGRFLVGNAAIKSSLPLKAFDVNDFTG
                     KSDNDLAIILTLSMIAAQRISLAVKNGEDLSDQLSTEINMTTALPVSEGKKNGIINNYV
                     NKYIGSKHTVVFHNFKDPITVSLSFKNVYVALEGEVAQLYLKNSDIKLQGLIKQDFSKN
                     YPELANDIKVSDLVKIDNLLGIDIGEGTTDLVVIKEGHANAVASTSLPTGYGNALQDAI
                     DVLQTENMNFEARSQLQNYLAQEVSPLAKRMQTKVRQIVFEQLEPFADKIVTAASKTMR
                     KAGANVEILYVYGGGSIPMLEQTALRQKLSQKMKDFSGGIDVPVIWINKSYAQNLNEKG
                     LELILKAMNK"
     CDS             1376..1765
                     /locus_tag="ctg71_3"
                     /transl_table=11
                     /translation="MPRPTKDPSTKSYTITVDGIYREWMDSQENKSVSIKNLIKLALEL
                     VGDGDFTEALIKKAASTSAYSEKASQVISNFNEEKVADTAHQQKLDDVNQKLADLQAER
                     EALLNEKADNTTGIDLSMLSTKFDK"
     CDS             2365..2667
                     /locus_tag="ctg71_4"
                     /transl_table=11
                     /translation="MNKIIISKLNNDENKIEWRISNSETGHYLNISISRALEDAMKKKR
                     NLSFNRFESEQINNLSHLVTNIQEDYVLNIDESNISSSYLPLKGIDALSYMKTVE"
     CDS             2926..3804
                     /locus_tag="ctg71_5"
                     /transl_table=11
                     /translation="MYYNAALVLEGGAMRGLYTSGVLDTFLEHDIQFDCVIGVSAGSLN
                     GANFVSKQYQRTYNVNVKYRKDHDYISLARPFKHESIINLDFLFDDHGLDWHNFDNKAY
                     LKSETDFIVVATSLESGKAVTLRNPQPGQDLIDALEASSSMPFISKPHNTSKGLCLDGG
                     IANSIPFDIAQKMGFQKIVVVRTRPRDYRKKPTSFALKRLYSNSFKEYPEFVKTAIRRP
                     EMYNHSVEVLNKLEHNDEIFCLAPKDPVKVGRLEHNTKKMTELYNIGKNDAENNLEAML
                     NYLQKSEPLYD"
     CDS             complement(3890..4726)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 70.1;
                     E-value: 7.7e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg71_6"
                     /sec_met_domain="Radical_SAM (E-value: 1.1e-33, bitscore:
                     106.1, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSANPLLNPVPTKDGHVVGYVHSLETFGAVDGPGIRFVAFMQGCH
                     MRCKFCHNPDTWKTRVGSQMTTEEVLNKALPYRSFWGDKGGITLSGGEILLQIDFALEL
                     FKMCKEEGISTCLDTCGQPFTRREPWFSKFNELMDYTDILLVDIKHINSDEHKRLTGFP
                     NDNILDMCEYLSSIGKPVWIRHVLIPGITDNDEYLKQLGEYVKENLHNVEKFEVLPYHT
                     MGVHKYQEMGIRYRLEGVEPPTPERVKNAEKLLEVDRYQGYKDWYPGINTLDQQPS"
     CDS             complement(4753..7020)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg71_7"
                     /sec_met_domain="Gly_radical (E-value: 2.6e-41, bitscore:
                     129.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.7e-261, bitscore:
                     857.9, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVEKTQMQNVDVKDAWEGFRGKEWKEEIDIREFIQDNFTPYDGDQ
                     SFLEGPTEATTKLNDKLIDLKLKERAHGGPLEADTKVVSTITSHKPGYLDKSLEKIVGL
                     QTDKPMKRALMPYGGIRMAKGALEAYGFKIDPETDFVFENLSKTHNQGVFDAYTSEIMK
                     ARHNKIITGLPDAYGRGRLIGDFPRIALYGIDRLIEGKKKDLENCGDGEMTNDVIQMRE
                     EISDQIKALNDMKIMAESYGYDISKPATTAKEAIQWLYFGYLASIKQQNGAAMSIGRID
                     SFIDIYIERDMKRGILTEKEAQELIDQFTMKLRMVCFIRTPAYNSLFSGNPIWATLSIA
                     GMGLDGRHHVTKTSYRFLNTLHNMGAAPEPNITLLWSDRLPESFKHYAASVSIASSTIQ
                     YENDDLMMKTWGTDYYGIACCVSAQPIANGMQFFGARANLAKAVLYAINGGKDELTKQQ
                     VGPAYEPISSEYIDYDEFMKKFDDMMGWIADIYVNALNTIHYMHDKYYYESAQMALKDT
                     LVDRSFATGISGLSVAADSISAIKYGHVKVIRDEDGIAVDFKADHDFPRYGNDDDRVDD
                     IAAWLVKELFTKMSKHHLYRGAKLTTSVLTITSNVVYGKNTGTTPNGRQKGEPFAPGAN
                     PAYGADTTGALASLLSVAKLPYKYATDGISNTFAVTPTTLGHDEKMQEDTLVNMIGGYM
                     RKDGHHLNINVFNRDTLLDAQAHPEKYPTLTIRVSGYCVYFADLTKEQQDDVIARTFHA
                     SM"
     CDS             complement(7332..7916)
                     /locus_tag="ctg71_8"
                     /transl_table=11
                     /translation="MKGMHVLRVKTNFFWDSLNVIRASILGANDGIISVSGIVLGAAGA
                     NLDSKTLLISGLSGMLAGACSMAGGEWISVSTQRDIQEKTMERQSQLENEDCENCPIKL
                     QKNDILMPFHAAASSFCSFIIGAMIPLLTMILARPEYRVVFTLIAMIASLSINAIVCTH
                     NSKVSTSKTILRNVITGLLTTLVTFILGASV"
ORIGIN
        1 caatacaagg tttaataagc attaaaaaca ttttaaaagt ttttataata ttaatagttg
       61 atttatactt aattacatgt ataattaaag gcggattgat gttgatgtag aaatggggta
      121 ttttctatgt caaaaaataa tattttaaaa ttgaatgttg ctaatgattt aggctatgga
      181 agtgttaagg ctaaagttga agatactaat attcactttc cttcagtaat tgctattcaa
      241 cgtgaacaag atttaaataa acccgttgaa ttcaattcaa atcaagaaaa attaacttac
      301 ttagaaggta tgattaatca tatggatgta accatatcta gttcagcagt aaagactcag
      361 ggtcgttttt tagtcggtaa tgcagctatt aaaagttcgt tacctttaaa agctttcgat
      421 gtaaatgact ttactggtaa atctgataat gatctagcta tcatcttaac tttaagcatg
      481 atagctgcac aacgtatttc tttagcagtt aaaaatggtg aagacttatc cgatcaactt
      541 agtacagaaa taaatatgac aacagctcta ccagtttctg aaggtaaaaa gaacggaatt
      601 atcaataatt acgttaataa gtatattggc agtaaacata ctgttgtatt tcataacttc
      661 aaggatccta ttactgtttc tttgagtttt aagaacgtat atgttgcttt agagggtgaa
      721 gttgcccaat tatatttgaa aaatagtgat attaaactac aaggattaat taaacaagat
      781 ttttcaaaaa attatcctga acttgctaat gatattaaag ttagtgattt agttaaaatt
      841 gataatctac taggaattga tatcggcgaa ggaactactg atttagttgt tattaaagag
      901 ggtcatgcta atgcagttgc ctctacttct ctacctactg gttatggtaa tgctttgcaa
      961 gatgcaattg atgtgctaca aacagaaaat atgaactttg aagcacgctc ccaactacaa
     1021 aattacttag cacaggaagt ttcaccttta gcaaaaagaa tgcagactaa agttcgccaa
     1081 attgtctttg aacaacttga accttttgct gataaaatag ttactgcagc aagtaaaaca
     1141 atgagaaaag ccggagcaaa tgtggaaatc ttgtatgttt acggtggtgg atcaattcct
     1201 atgttggaac aaactgctct acgacaaaaa ctttctcaaa aaatgaaaga tttttctggt
     1261 ggtattgatg ttcctgttat ttggattaat aaatcctatg cacaaaatct aaacgaaaaa
     1321 ggtttagaac ttattttaaa agctatgaat aaataaaaat tggaagtgat attttatgcc
     1381 aagacctaca aaagatcctt caacaaaatc ttatactatt actgttgatg gtatttaccg
     1441 tgaatggatg gattcacaag aaaacaaaag tgtatcaatt aaaaatttaa taaaactagc
     1501 tcttgaatta gttggtgatg gtgattttac agaagcttta ataaaaaaag ctgctagcac
     1561 ctctgcatat tcagagaaag cctcacaagt aatatccaac ttcaatgaag agaaagttgc
     1621 tgatactgca catcagcaaa agcttgatga tgtgaatcaa aaattagctg atctacaagc
     1681 tgaacgtgaa gcgttattga atgaaaaagc agataatact actggtatag atttatctat
     1741 gctatctaca aaatttgata aataagtaag tcctagagcc gtctagggct tttttattgc
     1801 aaaaaaataa cctagctgct gctaggcttt accgaattga aaggttccta tgttttttta
     1861 tctaattaat tgttgttttt ttgttaaatc ctaagaattt tgagttctga ttaagttctg
     1921 agtcaatagt tattaattac tattttttga atttactata tactgttaaa tcctatcttt
     1981 tccttatttg acagtatttg attatttttg agtttaaata agtaaaatca tattttttca
     2041 gaaagttaaa ataacttttt attttattgg aaattaattt cctaattttg attttaataa
     2101 tccgcctacg atctgctgtt tttaaaggca aatttgtata ccattaattt gctatacaac
     2161 tgtttccatt caataattct tcatcaaaac aattatatta acataccaat ccttatatta
     2221 tatggatatt caacttttta aataagcaaa aacaattatt tacttatatt tttaatttta
     2281 cccttgcttt tattcgtaaa taagagtaga gttatacttg taagttaagg aaagcattta
     2341 catttattaa ggtggtaatt atatatgaat aaaattatta ttagcaagtt aaacaatgac
     2401 gaaaataaga ttgaatggcg tatttctaac agtgaaactg gacattactt aaatatttca
     2461 atttcccgtg cactagaaga tgctatgaag aaaaagcgta atttatcctt taaccgcttt
     2521 gaatctgaac aaattaacaa tttgtctcac ttagttacaa acattcaaga agactatgtt
     2581 cttaatattg atgaatcaaa tattagctct agttacttac cattaaaggg tatcgacgca
     2641 ttatcatata tgaaaaccgt cgaataagtg attataagta ttaaattaag ttgttatttt
     2701 aatatttatc ttagatatac ttaagtattc ttaattgatt gaagatttgt ctgtcaacaa
     2761 cctctatttt aatgatagag gttgtttttt ttgttaaata ttttacagtt gcattgtttt
     2821 tggctaatta aatatttaaa ttctcttatt attcctttgt attcttgcta tctctacttt
     2881 ttaggtttat attaattcta gttatttttc gaaaggaatg gttacatgta ttacaatgca
     2941 gctctagttt tagaaggtgg tgcaatgcgt ggcctctata cttctggcgt tctagataca
     3001 tttttggaac atgatattca atttgattgt gttattggag tttctgcagg gtctctaaat
     3061 ggcgctaatt tcgtttcaaa acaatatcaa agaacttata atgtaaatgt taaataccgt
     3121 aaagatcatg actatatctc cttagcacgc ccttttaagc atgaaagtat tattaactta
     3181 gatttcttat ttgatgatca tggattagat tggcataatt tcgataataa agcctattta
     3241 aagtctgaaa ctgattttat tgttgtagct acttctctag aaagcggaaa agctgtaacg
     3301 ctacgtaatc cccaacctgg tcaagattta attgatgcct tagaagcttc ttcatctatg
     3361 cctttcatct caaagccaca taatacttct aaaggacttt gtctagatgg tggtattgct
     3421 aactctatcc cttttgatat cgctcaaaaa atgggttttc aaaaaattgt agttgttcgt
     3481 actcgtccac gtgattatcg caaaaagcct actagtttcg ccttaaaacg tctctacagt
     3541 aatagtttca aggaataccc agaattcgtt aaaacagcta tcagacgccc tgaaatgtac
     3601 aatcattctg ttgaagtttt aaataaactt gaacacaacg acgaaatttt ttgtttagca
     3661 cccaaggatc cagttaaagt tggtagactt gaacacaata ctaaaaagat gactgaactc
     3721 tataatatag gtaaaaatga tgctgaaaat aatcttgaag caatgcttaa ctatcttcaa
     3781 aaaagtgaac ccctatatga ctaaaataca ttttagaagc aaaaaacatg atatctactt
     3841 agtaaaacta agcgatacca tgttttttat tttatatttg ccaatttagc tagcttggtt
     3901 gttgatccaa agtattaatg cctggatacc aatctttata tccttgatat ctatctactt
     3961 ctagcagttt ttcagcattt tttactcttt ctggcgtagg aggctcaaca ccttcaagac
     4021 gatatctgat acccatttcc tgatacttat gaacgcccat tgtatgataa ggcaatactt
     4081 caaatttttc gacattatga agattttcct taacgtactc gcccaattgt ttcaagtatt
     4141 catcattatc cgtgatgcct gggataagga catgacgaat ccaaacaggt ttccctatac
     4201 tagaaagata ttcgcacata tccaaaatat tatcatttgg gaatccggtt aatctcttat
     4261 gttcgtccga attaatatgc ttgatatcaa ctaatagaat gtcggtatag tccattaact
     4321 cattgaactt actaaaccaa ggttctcttc ttgtaaatgg ctgtccacaa gtgtccaaac
     4381 aagtgctaat accttcttcc ttgcacatct tgaataactc tagcgcaaaa tctatttgca
     4441 atagaatttc accacctgat aaagttattc cacctttgtc gccccaaaag ctgcgataag
     4501 gaagtgcctt gtttagaact tcttcagtcg tcatttgtga acctaccctt gttttccaag
     4561 tgtccggatt gtgacagaac ttacacctca tatggcaacc ttgcataaaa gcgacaaaac
     4621 gtatacctgg tccatcaaca gctccaaaag tttccaaaga atgtacatag cctacgacat
     4681 gaccgtcttt agttggaact ggattcaata aaggattagc actcattctc tccacctact
     4741 ctcttctaaa tattacattg aagcatggaa ggttcttgca ataacgtcat cttgttgttc
     4801 tttagttaaa tcagcaaagt atacacagta acctgaaaca cgaattgtta atgttggata
     4861 cttctctgga tgtgcttgag catctaataa agtatctctg ttgaatacat taatattcaa
     4921 atgatgacca tcttttctca tatagcctcc gatcatgttt accaatgtat cttcttgcat
     4981 cttttcatca tgacccaatg ttgttggtgt tacagcaaat gtattagaaa taccatctgt
     5041 agcatacttg tatggtaatt tagcaacgga taatagtgat gctaaagcac ctgttgtatc
     5101 tgctccgtat gctgggttag cacctggtgc aaatggttca cccttttgac gaccatttgg
     5161 agtagtacct gtgttcttac cataaacaac atttgaagta attgtaagaa cggatgttgt
     5221 taacttagct ccacgatata gatgatgctt gctcatctta gtaaatagtt ccttaacaag
     5281 ccatgcagca atatcgtcaa cacgatcatc atcgttacca taacgaggga agtcatgatc
     5341 tgccttgaag tcaacagcaa taccatcttc gtcacgaatt actttaacat gtccgtactt
     5401 gatagctgaa attgagtcag cagcaacaga caatccagag atacctgtag caaagctacg
     5461 atctaccaat gtatctttca atgccatttg tgctgattca tagtaatact tgtcatgcat
     5521 gtaatgaatt gtgttcaaag catttacgta aatatcagca atccatccca tcatgtcatc
     5581 gaatttcttc atgaattcat cgtagtcaat gtattctgag ctaattggtt cgtatgcagg
     5641 acctacttgt tgttttgtaa gttcatcctt accaccattg atagcataca atacagcctt
     5701 agctagattt gcacgagctc cgaagaattg cataccattt gcaattggtt gtgcagatac
     5761 acagcaagca atgccatagt agtctgttcc ccatgtcttc atcattaagt catcgttttc
     5821 gtattgaatt gtagaacttg caatagatac acttgcagca tagtgcttaa atgattcagg
     5881 caaacgatca gaccaaagta atgtgatatt tggttctggt gcggcaccca tattatgcaa
     5941 tgtgtttaag aaacgatatg atgtcttagt tacatggtga cgtccatcta agcccatacc
     6001 agcaattgat aatgttgccc agatagggtt acctgagaat agtgagttgt aagctggtgt
     6061 acgaatgaag cataccattc tcaatttcat tgtgaattgg tcaattaatt cttgtgcttc
     6121 tttttcagtt aaaataccac gcttcatatc acgttcaatg taaatatcga tgaatgaatc
     6181 gatacgacca atagacatag cagcaccatt ttgttgcttg atagatgcta agtaaccaaa
     6241 gtatagccat tgaattgctt ctttagctgt tgtagctggt ttagaaatat catagccata
     6301 ggattcagcc ataatcttca tatcgtttaa ggctttgatt tggtcagaaa tttcttcacg
     6361 catttggatt acgtcgttag tcatttcacc atcgccgcaa ttttccaaat ccttcttctt
     6421 tccttcgatc aaacgatcaa taccgtataa tgcaatacgt gggaagtctc caatcaaacg
     6481 gccacgacca tatgcatctg gtagtccagt gataatcttg ttgtggcgag ctttcatgat
     6541 ttcgcttgtg tatgcatcaa atacaccttg gttatgcgtc ttagatagat tttcaaatac
     6601 gaagtctgtt tctggatcaa tcttgaatcc ataagcttct aaagcacctt tagccattct
     6661 gataccacca tatggcataa gtgcacgttt cataggttta tctgtttgta aaccaacaat
     6721 cttttctaat gacttgtcta aataacctgg tttatgagat gtaattgttg atactacttt
     6781 tgtatcagct tcaagaggtc caccatgtgc acgttctttt aattttaagt cgattaattt
     6841 atcgtttaac ttcgttgtag cttcggttgg tccttcaagg aaagattgat ctccatcata
     6901 tggagtgaag ttatcttgaa taaattcacg aatatcaatt tcttctttcc attcttttcc
     6961 tctaaatcct tcccaagcat cttttacatc aacgttttgc atttgagtct tttcaaccat
     7021 actcaaagcc tcctttgtga tttttaagaa aacgttttct acactgttaa tactacacct
     7081 tagttgaatt aaattcaatg aaaaatcgtt cacaatgttt tttattttca aaaaatctta
     7141 taaacgctat catttatttt aattattaga aatgttaata tgacaatact tttgacaaca
     7201 gtttataaac tttaaaaatg tgatttatgt atcaaaatat aaatttcatt aattttattt
     7261 tagaatttct taaaattaca aaaaaagacc ctatatcccc aaaaatttag gtatagagtc
     7321 tttaaaaata tttatactga tgcccctaaa ataaaagtaa ctaacgtcgt caacaatcca
     7381 gttataacat ttcgtaaaat tgttttgcta gttgaaacct tggagttgtg tgtacaaact
     7441 attgcattaa ttgataatga ggcaatcatc gcaattaatg taaataccac gcgatattct
     7501 ggtcttgcta ggatcatcgt caataatgga atcattgctc caattataaa tgagcagaaa
     7561 gaactggctg cagcatgaaa cggcattaag atatcattct tttgcaattt tatcggacaa
     7621 ttttcacagt cttcattttc caactgactt tgtctttcca ttgttttttc ttgaatatct
     7681 ctttgagtac ttacagatat ccattctcca ccagccattg aacaagctcc agctagcatg
     7741 ccagacaagc cactgattaa aagagtctta ctatcaagat tagcccctgc agcacctaac
     7801 acgattccag atactgaaat aatcccatca ttggcaccta aaatactagc tctgataaca
     7861 ttcaaactat cccagaaaaa attagttttt actcttaata catgcatacc tttcattttg
     7921 tacacttcct cacaaatttc gcaaaaatat cattttttat atatactgtc gaatatatgt
     7981 aaaccgcttt atttacgatt agattataca ttatattagg atttattact aatttagcaa
     8041 aacttataat tatatacatg tttcacaatg ttataaaaaa aggttgtata atatttagga
     8101 ctgataatgt aaaatttttt tgcattacgg tccttctttt attttctcca tttttacggc
     8161 aaa
//
