LOCUS       c00077_gnlPro..         6972 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PIHCMCBM_77.
ACCESSION   c00077_gnlPro..
VERSION     c00077_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-03 18:50:37
            Original ID  :: gnl|Prokka|PIHCMCBM_77
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6972
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6972
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3764:6971]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3765..6971
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..6972
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6972
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             complement(163..747)
                     /locus_tag="ctg77_1"
                     /transl_table=11
                     /translation="MTTKLKDLFDTFLNIAASSQKLGINLIRVAILIIFVWIGGLKFYN
                     YEAEGIVPFVANSPFMSFFYAKSAPEYKEYKLKEGEFNEAKHQWHVENNTYIFSHGLGI
                     MIMAIGILTFLGIFSPKIGLIGSGLAIIMTLGTLSFLVTTPEVWVPDLGSGEHGFPLLT
                     GAGRLVIKDTAIIAGAIVVLSDCAQRILKKK"
     CDS             complement(925..2139)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Radical_SAM"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg77_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.4e-14, bitscore:
                     43.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTKRTKSSFRTMSTNRKLESVFLFVTGRCNAKCKMCFYAKDMDK
                     KEKDLTFEEIKTISETAGQFNRLWVSGGEPTLREELPEIIEMFYKNNEIKDVNIPTNGL
                     KPDRIVAWVKQIRRNCPELNINLSLSLDGFGDTHDIQRGVPGNFYKAMGTLKLIEKNFA
                     DDGRVLKNLATVITKYNENEVEDLMIWMLGRFHLSNHIIEAARGMTRDDGVKVLTEPTL
                     RALQDKVVPIYNVYGERMAEETGGFRKPIARFFYLGFIRTLYNVRATNIDHPTPWGMDC
                     TAGETTLVIDYDGRFRACELREPLGNVKDYDCNIAKIMESDAMKQEIAAIGHGDKANCW
                     CTHGCWITSSLVFNPRKMITQVYKGYREMKKLNRPLNISEELLQSIERKYALDEQKLVE
                     LGVRN"
     CDS             2380..3225
                     /locus_tag="ctg77_3"
                     /transl_table=11
                     /translation="MEDNIPKFDLPVDFIVGNSITGEILNQYGRFPCKIKAGIFVLCVQ
                     GTVHATVNLTEFTIRKNDFITLPPGCFIQIHEVSDDVKLCFAGFSSVFVSHINYIKTIT
                     NYLPVIFDSPIMPLPEHISLLYQQAFDLLINAYSMPKTIENKEIIKAIFTIFMQGVIEL
                     YRNHTPYSNAPMTRNMEICREFVQLAMENYTKEHSVSYYAKRLNITLQHFCYVIKKVSG
                     RTALDILSNIIIMDAKAQLKSTDMPVKKIAFALGFDNLSFFNKYFRQHVGMTPQEYRES
                     "
     CDS             complement(3222..3662)
                     /locus_tag="ctg77_4"
                     /transl_table=11
                     /translation="MLLVFLICYTTLISILTIDKLENTCIFVLWFFXXXXXXXXXXXXX
                     XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
                     XXXXXXXXXXXXXXXXXXXXXXXXXXXXLIVYRGLRPAYPFF"
     CDS             complement(3765..4520)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg77_5"
                     /sec_met_domain="Fer2_3 (E-value: 4.7e-27, bitscore: 83.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 1.6e-12, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.7e-06, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDIKVTLKVWRQKSPKDKGQFETYQIDKINQSVSFLEMLDILN
                     ERLVSEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFNDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFVSVNTGGVPDANSIAIPRADAEMAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(4548..6800)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 162.5; E-value:
                     5.8e-50)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg77_6"
                     /sec_met_domain="FAD_binding_2 (E-value: 6.5e-51, bitscore:
                     162.5, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 6.7e-39,
                     bitscore: 121.8, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQIDSKIPQGPLAEKWKNYKDHQKLVNPANKRRLDVIVVGTGLA
                     GASAAASLAEMGFNVLNFCIQDSPRRAHSIAAQGGXXXXXXXXXXXXXXXXXXXXXXXX
                     XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
                     XXXXXQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKGGDYRAREANVYRLAE
                     VSNSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQTGQQLLLGAYSALSRM
                     IGLNKVKMYTRHEMLDVVKVDGRARGIIARNLITGQIERFSAHAVVVATGGYVNTFFLS
                     TNAMASNGSAAWQCYKKGAYFANPCMVQIHPTCVPVKGDYQSKLTLMSESLRNDGRIWV
                     PKKLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDVASRAAKERCDAGFGV
                     NNTGLAVFLDFSDAINRLGKDVVKAKYGNLFDMYEEITNDDPYETPMMIYPALHYSMGG
                     LWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYFVLPYTIQNYLSDQIQ
                     VPRFSTDLPEFAEAEKGIKDRIKKLMSIKGKESVDSIHKKLGHIMWEHVGMARDAKGLQ
                     EAIQMLKELKKEFWSNVYIPGEADNLNVELDKALRLADFIEIGTLMAHDALDRAESCGG
                     HFRTEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNYQFVVPQTRNYKK"
     CDS             complement(6834..6971)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg77_7"
                     /sec_met_domain="SucD_FumR (E-value: 3.6e-10, bitscore:
                     28.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="TIWMNRWICISKIVATVICVVFAIITIVFYLNGVGGDYVAVCAGH
                     "
ORIGIN
        1 ctaatactat tatgaaaaaa ccacgatgca aatatactgc ttaatccaaa cctgtcaaga
       61 ggttttatgc taaatagtgt ttcatacact ataagaaaag ctaacaaggt tcaatatgtt
      121 tgttttttgc ttccaaagtg ttaaaataaa cactttttgt tcctatttct tctttaatat
      181 acgttgtgca cagtcggaca ataccacgat cgctccggct atgattgccg tatcttttat
      241 taccagccga cctgctccgg tcagtaaggg gaaaccatgt tctccgcttc ccaaatcggg
      301 cacccatact tcaggtgttg tcaccaggaa tgaaagcgta cccaatgtca ttatgatcgc
      361 cagtccggaa cctatcaagc caattttcgg agaaaagata cccaggaaag tcaggatacc
      421 gatcgccatg atcataatgc ctagtccatg tgaaaaaata taggtattgt tttctacatg
      481 ccactgatgc ttggcttcat tgaattcacc ttctttcaac ttatattctt tgtattccgg
      541 tgcactcttt gcatagaaaa agctcataaa cggactgttt gccacaaaag gaacgatccc
      601 ttccgcttca taattataga atttcagccc gccgatccat acgaatataa tcaggatagc
      661 tacgcggatc aggttaatac ccagtttctg tgaagaagct gcaatgttca gaaatgtgtc
      721 gaataaatct tttaatttag tagtcatgtc tttctttgtt tatcagttta acttatgatg
      781 caaagatccg acatttttcc cctatgggaa atggcaattc taccggataa tttgacaatt
      841 attcccttaa acacaataaa gccggttaac agaggtatat ttcccgtcaa ccggctttat
      901 aaatgatccg ttatcggaac tattttaatt acgaaccccc agctccacca gtttctgttc
      961 gtccaaggca tatttgcgtt cgatgctctg caaaagctct tcactgatat tcagaggacg
     1021 gttcagcttc ttcatctccc ggtatccttt atagacctgc gtgatcatct tgcggggatt
     1081 gaaaacaagc gatgaagtga tccaacaccc atgcgtacac cagcagttag ccttgtcacc
     1141 atgaccgata gcggcaatct cctgtttcat ggcatcgctc tccataatct ttgcgatatt
     1201 gcaatcataa tcctttacat taccgagcgg ttcgcgaagt tcacaggcac ggaaacgacc
     1261 gtcatagtct attaccagtg ttgtttcgcc ggccgtacaa tccatacccc agggggtcgg
     1321 atgatcgata ttggtcgcac gtacattata taatgtacgt atgaaaccca ggtagaagaa
     1381 acgggcgata ggttttctga acccaccggt ttcctccgcc atacgctccc catatacatt
     1441 ataaatagga acaaccttat cctgcaaggc gcgtaaagta ggctcggtaa gaaccttcac
     1501 tccgtcgtcg cgggtcatgc cgcgtgcagc ttcgataata tgattggaaa gatggaaacg
     1561 tcccagcatc catatcatca gatcctcgac ctcattctcg ttatatttgg tgattacggt
     1621 ggccaggttt ttcaacaccc tgccgtcgtc agcaaagttt ttttcaatca gcttcagtgt
     1681 ccccatcgct ttgtagaaat tgccgggaac gccgcgctgt atatcatgcg tatcaccgaa
     1741 accgtccaaa gaaaggctca ggttgatatt cagttccgga caattccggc ggatctgttt
     1801 tacccaggcg acgatccggt caggcttcaa tccgttggta gggatattca cgtcctttat
     1861 ctcgttattc ttgtagaaca tctctataat ttcaggtaac tcctcacgca gtgtcggttc
     1921 accgccggat acccacaaac ggttgaactg cccggctgtt tcggagattg tcttgatttc
     1981 ttcaaaagtc aggtctttct cctttttatc catgtctttt gcatagaaac acattttaca
     2041 tttagcatta caccgtcctg tgacaaacag gaaaacagat tctaattttc tgtttgtgct
     2101 cattgtcctg aaagagctct ttgttctttt cgtactcatt gttattatcg attagatttc
     2161 tctattagta ttagctccca tatcttattt agcagataga caaatatata ccatcccaac
     2221 aaatctatca gcaagacttg aaaacattcg caaatttcaa ctattataca gcacaaaaca
     2281 atctccattt gtgggattcg atgttctata tttaagatta tcgactaact tgtctcaaaa
     2341 aaactatctt tgtacattca attaagtgta tataaaagta tggaagacaa tattcctaaa
     2401 ttcgacctcc ccgtagattt tattgtagga aacagtatta caggtgaaat actgaaccaa
     2461 tacggacgtt tcccgtgcaa gatcaaggca ggcatattcg tgctttgcgt acaagggaca
     2521 gtccacgcga ctgtaaactt aactgagttt accatccgga agaacgactt tatcacctta
     2581 cccccgggtt gcttcattca gatacatgag gtttccgatg atgtaaaact gtgttttgca
     2641 ggcttttctt ccgtattcgt cagccatatc aattatataa agacgattac gaactatctg
     2701 cctgtcatat tcgacagtcc gatcatgcct ttgccggagc atatatcctt actctaccaa
     2761 caggctttcg acttgctgat caatgcttat tccatgccta aaaccatcga gaacaaggag
     2821 attatcaagg ctatctttac tatcttcatg caaggggtta ttgaattata caggaatcat
     2881 acgccatata gcaatgctcc catgacgcgt aatatggaaa tatgccggga gttcgtacag
     2941 ctggcgatgg aaaactacac gaaagagcat agcgtgtctt attacgctaa aaggctcaac
     3001 atcaccttgc agcatttctg ttatgtgata aaaaaggtca gcggacggac tgcattggat
     3061 atcctgagca atatcatcat tatggatgcc aaagcgcagc ttaaatcgac cgatatgccg
     3121 gtcaagaaaa ttgcattcgc cctgggtttc gacaaccttt cgttcttcaa caaatatttc
     3181 cggcaacatg taggaatgac cccgcaggaa tacagggaga gctaaaaaaa gggatatgcc
     3241 ggacgcaaac ccctataaac aattaaagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnaaaa aaccacagta caaagataca ggtattctcc
     3601 aacttgtcaa tagttaaaat gctaattagt gttgtatagc atataagaaa cactaacaac
     3661 attcaagata tttatctatg acttcagaaa tgttaaaata ggggcaaaaa agaagaggcg
     3721 ctgccataag caacgcctct ccggacatac atatataatg tatattagtc ttgcaattta
     3781 gcacaaatga actcgcggtt caaacgagcg atgttgctga cagagatgtt cttcggacat
     3841 tccatttcgc aagcacgcgt gttggtacag ttaccgaagc ccagttcgtc catcttggca
     3901 accattgctt tcgcacgacg agcagcttct accttaccct gaggcaacag agccaactgg
     3961 ctaacctttg cagaaacgaa caacatagcc gaaccgttct tacaagcagc agcacaagca
     4021 ccgcaaccga tacatgcagc agcatccata gccatttcag catctgcacg aggaatagcg
     4081 attgaattgg catcaggtac accacctgtg ttcactgata cgaaaccacc tgcctggata
     4141 attttatcgt aagcataacg gtctaccatc aagtcgcgga ttaccgggaa accggcagaa
     4201 cgccacggtt cgattgtaat agtttcgcca tcattgaagc gacgcatata aagctggcaa
     4261 gtagttgcgc cggttgcagg tccgtgcgga tgtccgttta catacaaaga acacataccg
     4321 cagatacctt cacgacagtc gtgatcgaat acgatcggct ctttaccttc actgaccaaa
     4381 cgttcattca aaatgtctaa catttccagg aaagaaacac tctgattgat tttgtctatt
     4441 tggtaagtct caaattgtcc tttatctttc ggacttttct gacgccaaac tttaagtgtt
     4501 acttttatat ctttatccat gattctaatt tttgtgattg atgattatta cttcttgtag
     4561 ttacgtgttt gaggaacaac gaactgatag ttcaaatctt ccttcagcat aaccggatct
     4621 ttgtcttcac cctggtattc ccaacaagat acgtacatga acttatcgtc atgacgcagg
     4681 gcttcgcctt cctcggtctg gtgctctgta cggaagtgtc ctccacaaga ttcggcacgg
     4741 tccaatgcat cgtgtgccat caatgtacca atctcaataa agtcagccaa acgcaatgcc
     4801 ttatccaact ctacgttcag gttatcggct tcacccggaa tatatacatt actccagaac
     4861 tctttcttca gctctttcaa catctggatc gcttcctgca aaccttttgc atcacgtgcc
     4921 atacctacat gttcccacat gatatgaccc agtttcttat ggatagaatc taccgactct
     4981 ttacccttga tactcatcaa tttcttgatg cggtctttga tccccttttc agcttctgca
     5041 aactcaggca ggtcggtact gaaacgcgga acctggatct ggtctgacag atagttctgg
     5101 attgtataag gcaatacaaa gtagccatcg gccaatccct gcatcaacgc agaagcaccc
     5161 agacggttag ctccgtggtc agagaagtta gcttcaccga tagcgaacag acccggaata
     5221 gaagtcatca attcatagtc aacccacaga ccacccatcg agtagtgcaa tgcaggataa
     5281 atcatcatcg gagtttcgta tggatcgtcg ttggtaattt cttcgtacat gtcgaacaag
     5341 ttaccatatt tagcttttac aacatcttta cccagacggt tgatggcgtc ggagaagtcg
     5401 aggaacacgg ccagacctgt gttgttcaca ccgaagccgg cgtcgcaacg ttctttagcg
     5461 gcacgtgaag ccacgtcacg cggaaccagg ttaccgaatg ccggataacg gcgttccaga
     5521 tagtagtcgc ggtctgcttc aggaatgtct ttccctttct tagttccggc ctgtaatgct
     5581 tttgcatctt ccagtttctt cggaacccag atacgaccgt cgttacgcaa tgattcagac
     5641 atcaaagtca gcttagactg gtagtcacct ttcaccggta cacaagtcgg gtggatctgc
     5701 accatacaag ggttagcgaa gtaagcacct ttcttgtagc actgccatgc agcagaaccg
     5761 ttggaagcca ttgcgttagt agacaggaag aaagtattta cataaccacc tgttgccacc
     5821 actactgcgt gagcagaaaa acgttcgatc tgacctgtga tcaggttacg tgcgatgata
     5881 ccgcgggcac ggccatccac cttcacaacg tccagcattt cgtggcgggt atacatcttt
     5941 actttgttca aaccgatcat acggctcaaa gcagagtaag cacccagcaa caactgctgt
     6001 ccggtctgac cgcgggcata gaacgtacgg gatacctgag caccaccgaa tgaacggtta
     6061 tctagcagac caccgtattc gcgagcgaaa ggaacaccct gtgctacgca ctggtcgata
     6121 atagagttgg aaacttcagc cagacgataa acgtttgctt cgcgggcacg atagtcacca
     6181 cctttgattg tatcgtagaa cagacggtaa acagagtcac catcattctg ataattcttt
     6241 gcagcattga taccaccctg tgcagcgata gagtgtgcac ggcgcggaga gtcctgnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn accaccctgt gcggcgattg agtgtgcgcg acggggagaa
     6601 tcctggatac agaagttcaa tacgttgaat cccatttctg ccagagaggc agcagcagaa
     6661 gcaccggcca gaccagtacc aactacaatc acgtccaaac gacgtttgtt tgcggggttc
     6721 accagtttct gatgatcttt ataatttttc cacttctcag ccaacgggcc ctgagggatt
     6781 ttagaatcta tttgagtcat aatgatttat tttcaatatt gttacacctt aaattaatgt
     6841 cctgcacaaa cagctacata gtcaccacct acaccattca ggtagaaaac gatagtaatg
     6901 atagcaaaca ctacgcaaat aacggttgct acaatcttgg aaatgcagat ccaacggttc
     6961 atccagatcg tg
//
