LOCUS       gnlProkkaMKKKKJJF_21    5025 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MKKKKJJF_21.
ACCESSION   gnlProkkaMKKKKJJF_21
VERSION     gnlProkkaMKKKKJJF_21
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-30 20:40:10
            Original ID  :: gnl|Prokka|MKKKKJJF_21
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5025
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5025
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[2:3745]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3..3745
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..5025
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..5025
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     aSDomain        complement(3..491)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg21_1_Aminotran_5.1"
                     /evalue="3.40E-24"
                     /label="ctg21_1_Aminotran_5.1"
                     /locus_tag="ctg21_1"
                     /protein_end="193"
                     /protein_start="30"
                     /score="77.2"
                     /tool="antismash"
                     /translation="PREKEFAGMMKQLRDDLVRVAHGDLSKHTAVLFCGSGTINIDICL
                     NSLLPADKKVLVVNNGAYSTRAVEVCEYYGLPHIDLKFSVYELPDLSVIEKTLSDNPDI
                     ALVYTTHHETGTGILNPIREIGKIAHSHDAVFVVDTTSSLGMIPFDIGKDNVDFCMASA
                     "
     CDS             complement(3..581)
                     /NRPS_PKS="Domain: Aminotran_5 (30-193). E-value: 3.4e-24.
                     Score: 77.2. Matches aSDomain:
                     nrpspksdomains_ctg21_1_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 60.4; E-value:
                     2.4e-18)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg21_1"
                     /sec_met_domain="Aminotran_5 (E-value: 2e-23, bitscore:
                     71.2, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIERKILLNPGPATTTDTVKLAQVVPDICPREKEFAGMMKQLRD
                     DLVRVAHGDLSKHTAVLFCGSGTINIDICLNSLLPADKKVLVVNNGAYSTRAVEVCEYY
                     GLPHIDLKFSVYELPDLSVIEKTLSDNPDIALVYTTHHETGTGILNPIREIGKIAHSHD
                     AVFVVDTTSSLGMIPFDIGKDNVDFCMASA"
     CDS             complement(578..1714)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 71.8;
                     E-value: 7.9e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg21_2"
                     /sec_met_domain="TPP_enzyme_N (E-value: 7.5e-23, bitscore:
                     69.4, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2e-21, bitscore:
                     64.8, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVSVEKIYDTFLHHGVDFFTGVPDSLLKNICAYITDHAPRGKHII
                     AANEGSAVGIACGYYMASGNVPLVYMQNSGLGNTVNPLLSLADEKVYSLPLLLLVGWRG
                     EPGTKDEPQHKKQGEVTLDLLKAMKIPFIILDTDEDEALAQIHDAVVSAKSRSIPHAIV
                     IRKDTFGKYKLRQEALNDYPLSREEALMQVVDHLGDNDIVVSTTGKLSRELFEYRESKG
                     QGHARDFLTVGSMGHSSSIALGVALEKPDRRVYCLDGDGAFIMHMGAVSNIGDLSPKNY
                     RHVLFNNGAHESVGGQPTLGFRLDIASIAKGCGYAHVLTASDKEGLSCALEKLSGLSGP
                     VLLEIKVRIDSRDDLGRPTTTPVENKEHFMAFLKDTHS"
     CDS             complement(1726..3027)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 214.1;
                     E-value: 3.9e-65)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg21_3"
                     /sec_met_domain="CTP_transf_like (E-value: 1.3e-13,
                     bitscore: 39.9, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 4.6e-123, bitscore:
                     398.2, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 2.3e-65, bitscore:
                     208.8, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKKIYVGMTADIMHPGLIHIIHEATKYGDVIVGLLTDKAIAEHK
                     RLPYLTYDQRKEVVENIKGVCEVVPQEDWSYVPNLEKLKPDYIIHGDDWKTGPLREVRE
                     QVFEVMNAQGGKVIEIPYTRGINSSSLDKDIKAIGTTPDVRLKSLRRLIQAKPVVRILE
                     AHDGLCGLIIENLEVQKGDKREVFDGMWSSSLTDSTSKGKPDIEAVDLTTRLQDLNNIL
                     ECTTKPIIFDGDTGGKIEHFVFTVRTLERHGISAVIIEDKVGLKKNSLFGTDAIQTQDS
                     IEGFCDKIRAGKNAQVTGDFMIIARIESLIAGKPMSDALERAYAYVQAGADGIMIHSKD
                     KSGEDIREFCRTFRKEYAHVPIVVVPTTYDHVHESELHEWGANVVIYANHLLRAAYPAM
                     MNVARTILENERAEEVRPLCMPIKEILELIPGTK"
     CDS             complement(3047..3745)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg21_4"
                     /sec_met_domain="NTP_transf_3 (E-value: 3.9e-15, bitscore:
                     45.0, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAGLGTRFGHYTELVPKGFVEVGGKPMIIRSIETLLSCGIERII
                     LGTGYKKEAYEALQADFPQIETCFSPRYADTNSMYTLYNTRDVIGDDNFLLLESDLVFE
                     RKAILSLLDDEFPDVMLVSSLTKFQDQYYVEYDCNHILTSCSVDKDALEAKGELVGIHK
                     LSNTFYRRMCADYATILESQPKLGYEYELLRMSRSVSPVRVLRVEGLKWYEIDDEADLS
                     YAEEHIIRYC"
     CDS             complement(3759..5024)
                     /locus_tag="ctg21_5"
                     /transl_table=11
                     /translation="MRSPLYVLALYASVLIFLVYTCAYAYRKPFTAAIYEGEILWGFDV
                     KILYVLSEIIGYALSKFIGVRILPSMKAGHRIYYIIGLLTFSEVALLGFALLPVPLKVC
                     SIFLSGLPLGMIWGVIFSYIEGRRISEILNVGLSVALIVSSGLVKTLGQFVMDNLHVSE
                     YWMPFTTGLLVYPVMLLCSWLLNQIPAPNEQDIIQRTKRAPMDGKDRRKFLRQFFWGIC
                     MLVVFYGALTVFRELRDSFAADIWKELHIEGAMIFTQTEVPIAAFVLVLMFLIVFVRNN
                     RLALNIIYCIAVTGGSLMVFSTLLYVYGFLSPIWWMILSGLGLYMGYIPFTYLIERLIA
                     SLRVVSTAVFIIYLADSFGYLGTTGVFMVKNFVSADISWTVMLMRTALFSGFVSVLSIF
                     AIYCYFKQQLNSLILIPSEIHD"
ORIGIN
        1 tgagccgaag ccatacaaaa gtcaacatta tccttcccga tatcaaaagg aatcataccc
       61 agggaagaag tggtgtcgac aacaaaaacg gcatcgtgcg aatgggctat ttttccgatc
      121 tcacggatgg ggttcaagat acccgtaccc gtttcatggt gcgtggtata gaccaaagcg
      181 atgtccggat tgtcggaaag cgtcttttcg attaccgaca ggtcgggaag ttcgtaaacg
      241 gaaaacttca aatcgatatg gggaagaccg taatattcgc agacttccac cgcacgggtg
      301 ctgtaggcac cgttattgac aaccagaact ttcttatccg caggaagaag agagttcagg
      361 cagatatcga tattgatcgt acccgagccg caaaacagga cggccgtatg tttggagagg
      421 tctccatgcg caacgcggac caggtcgtca cgtaactgtt tcatcatacc cgcaaactct
      481 ttctcacgcg gacagatatc gggaacaacc tgcgccagct ttaccgtatc cgtcgtggtg
      541 gcgggacccg gattcaaaag gatctttctt tcgatattca tgaatgcgta tcttttaaaa
      601 atgccataaa atgctcttta ttctctaccg gagtagtcgt agggcgaccc agatcatcac
      661 gtgaatcgat ccggacctta atttcaagaa gaaccgggcc ggacaggccg gacagcttct
      721 caagcgcaca cgaaagccct tccttgtcgg aggcagtcag tacatgcgca taaccgcaac
      781 ctttcgctat cgaggcgata tccagacgga aaccgagcgt gggttgaccg ccgacagatt
      841 catgggcacc gttgttgaaa aggacgtgcc tgtaattctt gggagaaaga tcgccgatat
      901 tgctgacagc acccatgtgc atgataaagg caccgtcacc gtcaagacag tagacacggc
      961 ggtcaggctt ctcaagggca acccccaggg caatggaaga actgtgcccc atggaaccca
     1021 cagttaggaa atcccttgcg tgcccctgcc ctttcgattc acggtactcg aaaagctcac
     1081 gggagagctt gcccgtggtg gaaaccacaa tgtcattgtc cccgaggtgg tcgacaacct
     1141 gcattagcgc ctcctcgcgg gacagaggat agtcattcaa ggcttcctga cgcaatttat
     1201 attttccaaa cgtatcttta cggatgacaa tcgcatgggg aatcgaccgg ctcttcgccg
     1261 agacaaccgc gtcatggatc tgcgccaggg cctcgtcttc atccgtatcc agaattataa
     1321 aaggaatttt catggctttc agaagatcaa gggtaacctc gccctgcttc ttatgctggg
     1381 gttcgtcttt cgtgccgggc tcaccgcgcc agccgaccag caaaagaaga ggaaggctgt
     1441 acactttctc gtccgcaagg gacaagaggg gattcaccgt atttcccaga ccggagttct
     1501 gcatataaac caaaggaacg ttgccagaag ccatataata accgcaggca atgcccaccg
     1561 cggaaccttc attggcggcg atgatatgct taccacgcgg agcatgatcc gtgatatagg
     1621 cgcaaatatt cttcaaaagg gaatcgggaa cacccgtaaa gaagtcaaca ccgtgatgaa
     1681 ggaacgtatc gtatatcttt tctacactta ccataaccgt ctgatttatt tagtgcccgg
     1741 aatcaattcc agaatttcct tgataggcat acaaagcgga cggacctcct cggcgcgctc
     1801 gttttcaagg atggtcctgg ccacgttcat catggccggg taagcggcac ggagaaggtg
     1861 gttggcgtaa atgactacgt tggcacccca ttcgtgaagt tcggattcgt gcacatggtc
     1921 ataagtcgtc ggaacaacga caatgggaac atgggcgtat tctttgcgga aagtccggca
     1981 gaactcacgg atatcctcac ccgacttatc cttgctgtgg atcatgatgc cgtccgcacc
     2041 cgcctgtaca taggcataag cacgttcgag ggcgtcactc atgggtttgc cggcaataag
     2101 gctttcgata cgcgcaatga tcataaagtc accggtgacc tgggcattct tccctgctct
     2161 gatcttatcg cagaaaccct cgatactatc ctgagtctgg atagcgtcag taccaaaaag
     2221 agaattcttc ttcagaccga ctttatcttc aatgattacc gcagaaatgc cgtgacgttc
     2281 cagagtgcgc acagtaaaga caaagtgttc gatcttgcca cccgtgtctc catcaaaaat
     2341 aataggcttg gtggtacatt caaggatatt gttcaggtcc tgtaaacggg tggtaaggtc
     2401 gaccgcttca atatccggtt tacccttact cgtagagtcg gtcaggctgc tggaccacat
     2461 gccgtcaaag acctcgcgct tatcaccctt ctggacttca agattctcaa taatcagacc
     2521 gcaaagaccg tcgtgagcct ccaggatacg gaccacaggc ttggcctgga tcaggcggcg
     2581 gagggactta agacgtacgt caggggtagt cccgatcgcc ttgatatcct tgtcgagcga
     2641 ggaggagttg atgccccggg tataagggat ctcaataacc ttgcccccct gagcgttcat
     2701 cacctcaaaa acctgttcgc gcacctcgcg caacgggccc gttttccagt cgtcaccgtg
     2761 gatgatatag tcaggcttga gtttttccag attcggaaca tagctccaat cctcctgggg
     2821 aacaacttcg caaacccctt tgatattctc aacaacctcc ttacgctgat cataggtaag
     2881 gtaagggagg cgtttgtgtt cggcgatggc cttgtccgtc agcagaccga cgatgacatc
     2941 accgtattta gtagcttcat gaataatatg gatcagcccc ggatgcatga tatccgcggt
     3001 cataccgaca tagattttct tgttcatata tggaaaagat ttgtttttaa caatagcgga
     3061 tgatgtgctc ctcagcatac gaaaggtcgg cttcatcatc gatttcatac catttaagcc
     3121 cctccaccct gagcacacgg acaggggaaa cggaacggga catgcggagc agttcgtatt
     3181 cgtaacccag tttaggctgg gactccagga tggtcgcata atcggcgcac atccggcggt
     3241 agaaagtatt ggaaagtttg tggatgccga ccagctcgcc ttttgcctca agggcgtcct
     3301 tgtctaccga acaggaagta aggatgtgat tgcagtcata ttccacataa tactgatcct
     3361 ggaacttggt cagagaagag acaagcatca catccgggaa ttcgtcatcc agtagcgaga
     3421 ggatggcctt gcgttcaaaa acaaggtccg attcaagcag caggaaattg tcatcgccta
     3481 tcacgtcacg cgtattgtac agagtgtaca tactgttagt atcggcataa cggggactga
     3541 aacaggtctc tatctggggg aaatccgcct gaagggcttc ataagcctct tttttataac
     3601 ccgtaccgag gatgatacgt tcgataccgc aggaaagaag ggtttcgata gagcggataa
     3661 tcataggttt tccaccaacc tccacaaaac ctttgggcac aagctcggta taatgcccga
     3721 agcgggtacc aagaccggca gccatgatca ttgctgtttt aatcatgaat ttcagaggga
     3781 attaatataa gtgaattcaa ttgttgcttg aaataacagt aaatcgcaaa aatcgacagg
     3841 acagaaacaa atcctgaaaa aagggcagta cgcattagca tgacagtcca ggaaatgtca
     3901 gcagaaacga agttcttgac cataaacaca cccgtagtgc ccagatagcc gaaagaatcg
     3961 gccaaataaa taataaaaac agccgtacta accaccctaa gggaagcaat caggcgttct
     4021 atcaggtaag tgaaaggaat ataccccata tacagaccaa gaccggaaag aatcatccac
     4081 caaataggag aaagaaaacc gtacacataa agcaatgtgg aaaaaaccat taaggaaccg
     4141 cctgtgacgg caatgcaata aatgatatta agagccaaac ggttattacg gacaaacaca
     4201 atcaggaaca tcagtacaag gacaaaagcg gcaatgggaa cctctgtctg agtaaagatc
     4261 attgcaccct caatatggag ctccttccaa atatcggcag cgaaagagtc acgaagctca
     4321 cggaacacgg tcagggcacc gtaaaaaaca accagcatgc agattcccca aaagaattgc
     4381 ctcaaaaatt tacgcctgtc tttaccatcc atcggagcac gtttggtacg ctgtatgata
     4441 tcttgctcat tgggagccgg aatctgattg agaagccagc tgcaaagcaa cataacaggg
     4501 tataccaaca aaccggtggt aaaaggcatc caatattccg acacatgcaa attatccatc
     4561 acgaattgtc ccaaggtttt caccaaaccg gaagagacta tcaaagcaac gctcagcccg
     4621 acattcaata tttcactgat acgacgccct tcgatatagc taaaaatgac accccagatc
     4681 atacccaggg gaagaccgct cagaaaaata gaacacacct tcaaaggaac tggaagaagt
     4741 gcaaaaccca acaaagcaac ttcggaaaaa gtaagcagcc ctattatata atagatgcgg
     4801 tgacccgctt tcatcgaagg cagaatacga acaccgatga acttggaaag ggcgtagccg
     4861 ataatctcag aaaggacata caggattttg acatcgaatc cccaaaggat ctcaccttca
     4921 taaatggctg cggtaaatgg cttacggtag gcataggcac aagtataaac cagaaaaatc
     4981 aaaacgctgg catagagagc caaaacgtaa aggggagaac gggaa
//
