LOCUS       gnlProkkaBGMHMDPJ_32   12741 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BGMHMDPJ_32.
ACCESSION   gnlProkkaBGMHMDPJ_32
VERSION     gnlProkkaBGMHMDPJ_32
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2025-04-28 06:26:31
            Original ID  :: gnl|Prokka|BGMHMDPJ_32
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12741
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..12741
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[2947:10850]"
                     /cutoff="10000"
                     /detection_rule="(HMGL-like and AMP-binding)"
                     /neighbourhood="10000"
                     /product="betalactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2948..10850
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(HMGL-like and AMP-binding)"
                     /neighbourhood="10000"
                     /product="betalactone"
                     /protocluster_number="1"
     cand_cluster    1..12741
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(HMGL-like and AMP-binding)"
                     /kind="single"
                     /product="betalactone"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..12741
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="betalactone"
                     /region_number="1"
                     /rules="(HMGL-like and AMP-binding)"
                     /tool="antismash"
     CDS             complement(467..1348)
                     /NRPS_PKS="Domain: PKS_ER (11-220). E-value: 2.8e-12.
                     Score: 38.2. Matches aSDomain:
                     nrpspksdomains_ctg32_1_PKS_ER.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040:alcohol dehydrogenase (Score: 188.6; E-value:
                     2.3e-57)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg32_1"
                     /sec_met_domain="ADH_N (E-value: 1.4e-12, bitscore: 37.2,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 1.9e-22, bitscore:
                     69.4, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFEDALGPRENIILGHEACGVVVEVGEDVKDFKPGDRVLVPAITP
                     NWSDVYSQAGYATDSDGMLGGWKFSNIKDGVFATRFHVNDADGNLAHLPDEIDPTEACM
                     LSDMIPTGLHASEMAGVTFGDAVAVIGIGPVGLMALRGAVLHGAGRVFAVGSREKTVEV
                     AKKYGATDIIDYHNGRISDQILEATGGQGVDKVLIAGGNAKDTFEEAVRMLKPGGSIGN
                     VNYLNGHDDVVFNAGDWGVGMGHKTIHGGLMPGGRLRMEKLAVLVVNGRIDPSLMITHR
                     FEASRRFRTPSN"
     aSDomain        complement(689..1315)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg32_1_PKS_ER.1"
                     /evalue="2.80E-12"
                     /label="ctg32_1_PKS_ER.1"
                     /locus_tag="ctg32_1"
                     /protein_end="220"
                     /protein_start="11"
                     /score="38.2"
                     /tool="antismash"
                     /translation="IILGHEACGVVVEVGEDVKDFKPGDRVLVPAITPNWSDVYSQAGY
                     ATDSDGMLGGWKFSNIKDGVFATRFHVNDADGNLAHLPDEIDPTEACMLSDMIPTGLHA
                     SEMAGVTFGDAVAVIGIGPVGLMALRGAVLHGAGRVFAVGSREKTVEVAKKYGATDIID
                     YHNGRISDQILEATGGQGVDKVLIAGGNAKDTFEEAVRMLKPGGSI"
     CDS_motif       complement(884..910)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg32_1_0002"
                     /evalue="5.10E+00"
                     /label="PKSI-ER_m2"
                     /locus_tag="ctg32_1"
                     /protein_end="155"
                     /protein_start="146"
                     /score="0.9"
                     /tool="antismash"
                     /translation="GAGRVFAVG"
     CDS_motif       complement(1271..1309)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg32_1_0001"
                     /evalue="5.80E-03"
                     /label="PKSI-ER_m2"
                     /locus_tag="ctg32_1"
                     /protein_end="26"
                     /protein_start="13"
                     /score="9.7"
                     /tool="antismash"
                     /translation="LGHEACGVVVEVG"
     CDS             complement(1381..1476)
                     /locus_tag="ctg32_2"
                     /transl_table=11
                     /translation="MKGFAMLGPNKTGFIEKEDPKVGSRDAWYVP"
     CDS             complement(1707..2897)
                     /gene_functions="transport (smcogs) SMCOG1106:major
                     facilitator transporter (Score: 231.1; E-value: 2.9e-70)"
                     /gene_kind="transport"
                     /locus_tag="ctg32_3"
                     /transl_table=11
                     /translation="MDAKPNRRALSLGSFVILYLGYMLLFADRTVLNLSLAAIGKDFSV
                     SPAALGATSSVFFLGYTIMQIPGGWLTDRFSSRKVIIATLAFWSVMTIFTGLAWSLAAL
                     LVIRFLFGIAEGPYPPAAMKQVAQDFPVNKRAQLTAALISSNYAGAALAPFIIVPIIAA
                     TGWRSAFFWLGGFGLLLMVSYLFFRGHGGGQTVTKQERQKINWKEIDPRVWAFVVIGLA
                     LNIITKGMETWMPVYFLNARHINLANLAWLVPLPTIAGGIAALISGYVMVHIFKNHERL
                     MIILASFLTVLTMFGLYKSTSLVWLVIFQMATYFVKSLAFTGIFSYAAQLFPQKTYGSQ
                     IGTINFGGQLGGFFGPLLIGWIVQLSGSYDTAFFGLVISALVAGVASLFVGNLKAK"
     CDS             complement(2948..4372)
                     /NRPS_PKS="Domain: AMP-binding (8-379). E-value: 3.2e-71.
                     Score: 231.9. Matches aSDomain:
                     nrpspksdomains_ctg32_4_AMP-binding.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     307.1; E-value: 3e-93)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg32_4"
                     /sec_met_domain="AMP-binding (E-value: 6e-76, bitscore:
                     245.2, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIPNWLLKQASLNPNRVAVNDGTTAYTFAQLVPLVAHRAGQIQA
                     QTDQPRVGVMTHNNLAGYLAALGVLAADKTIVWLNWRLAPEELSRQIGDAGLDLVLVDD
                     QLADRYYGGHRVLCSELTAGTAPAAVLTPTVDLDAVASIMYTSGTTGQPKGVLQTYGNH
                     LASAISSSLNLGVLPNDLWLCVAPIFHISGFSILMRGLIYGMGVRLVNHFDAQVVNQIL
                     EDEPVTIISAVPYMLKKMLEDVQANDHCYSSAFRLVLLGGGTIDRATLNACEESGLPVV
                     QCYGMTETCSQFVALSAEEAPYHLGSVGKPLFTTQLRLVGEHHEIEIQTPALTPGYLNQ
                     PAKFAAKHDGDWYRTGDVGHFDEDGFLYVDGRLDEMIISGGENIFPAEVENVLRQYPGI
                     QEIAVVGRNDQQWGAVPVAVVVSKQPVDFQQLEAFGREHLAHYKVPKVYLQTDALPKNA
                     SGKVQKFRLQDLVANA"
     aSDomain        complement(3236..4348)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg32_4_AMP-binding.1"
                     /evalue="3.20E-71"
                     /label="ctg32_4_AMP-binding.1"
                     /locus_tag="ctg32_4"
                     /protein_end="379"
                     /protein_start="8"
                     /score="231.9"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="KQASLNPNRVAVNDGTTAYTFAQLVPLVAHRAGQIQAQTDQPRVG
                     VMTHNNLAGYLAALGVLAADKTIVWLNWRLAPEELSRQIGDAGLDLVLVDDQLADRYYG
                     GHRVLCSELTAGTAPAAVLTPTVDLDAVASIMYTSGTTGQPKGVLQTYGNHLASAISSS
                     LNLGVLPNDLWLCVAPIFHISGFSILMRGLIYGMGVRLVNHFDAQVVNQILEDEPVTII
                     SAVPYMLKKMLEDVQANDHCYSSAFRLVLLGGGTIDRATLNACEESGLPVVQCYGMTET
                     CSQFVALSAEEAPYHLGSVGKPLFTTQLRLVGEHHEIEIQTPALTPGYLNQPAKFAAKH
                     DGDWYRTGDVGHFDEDGFLYVDGRLDEMIIS"
     CDS_motif       complement(3902..3952)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg32_4_0001"
                     /evalue="4.30E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg32_4"
                     /protein_end="157"
                     /protein_start="140"
                     /score="22.4"
                     /tool="antismash"
                     /translation="ASIMYTSGTTGQPKGVL"
     CDS             complement(4382..5203)
                     /NRPS_PKS="Domain: ECH (18-261). E-value: 2.8e-63. Score:
                     205.3. Matches aSDomain: nrpspksdomains_ctg32_5_ECH.1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023:enoyl-CoA hydratase (Score: 198; E-value:
                     2.4e-60)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg32_5"
                     /transl_table=11
                     /translation="MTSANWTPVKEYSEIIFERDDKIAKITMNRPEKHNAFTPVTVAEM
                     IDAFNICRDDSTIGVIILTGAGDKAFSSGGDQSVRGNGGYVGPDHIARLNVLDLQHLIR
                     IIPKPVIAMVKGWSVGGGNVLQLVCDLTIAADNAMFGQTGPKVGSFDAGYGSGYLARVI
                     GHKRAKEVWFLNHFYTADEAYQMNWINKVAPLAEVESVTLDWCHEILKKSPTALRFIKA
                     AMNADTDGLAGLQQFAGDATMLYYTTDEGKEGRDAFNQKRDPDFDKFPKFP"
     aSDomain        complement(4421..5149)
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg32_5_ECH.1"
                     /evalue="2.80E-63"
                     /label="ctg32_5_ECH.1"
                     /locus_tag="ctg32_5"
                     /protein_end="261"
                     /protein_start="18"
                     /score="205.3"
                     /tool="antismash"
                     /translation="RDDKIAKITMNRPEKHNAFTPVTVAEMIDAFNICRDDSTIGVIIL
                     TGAGDKAFSSGGDQSVRGNGGYVGPDHIARLNVLDLQHLIRIIPKPVIAMVKGWSVGGG
                     NVLQLVCDLTIAADNAMFGQTGPKVGSFDAGYGSGYLARVIGHKRAKEVWFLNHFYTAD
                     EAYQMNWINKVAPLAEVESVTLDWCHEILKKSPTALRFIKAAMNADTDGLAGLQQFAGD
                     ATMLYYTTDEGKEGRDAFNQK"
     CDS             5238..5621
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1144:thioesterase superfamily protein (Score: 100.2;
                     E-value: 1.1e-30)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg32_6"
                     /transl_table=11
                     /translation="MNLLENLGIHQDLVTKDRVELSMPVQAKHLQPYGIVHGGINTVLA
                     ETAASLGANANVAPGRIAVGVNVTTQHIRAIREGEIFVVATPDHVGRTLQTWQARLLVN
                     DRLTATATITLMNVPAPTNNHKH"
     CDS             complement(6030..6407)
                     /locus_tag="ctg32_7"
                     /transl_table=11
                     /translation="MTNDINFWTGIKDPYLKPDKPFLTEDKELKIIHLIQSYPMHCPLC
                     GQLMRRNGFRKRPVTIKILTPAGKPAVLKIKKQQYLCPPSPQCPKRVTKVAEIQGVNFA
                     CRIANVVKYHIVQELSESVRN"
     CDS             complement(6619..7398)
                     /locus_tag="ctg32_8"
                     /transl_table=11
                     /translation="MMYEFPRLTAHHIQTTDQPVVTSTNEMAKEQLKVKDQPFLISARQ
                     QTAGHGKTSRKFYSPAGSGAYFTLALPFSYLAKSAGQVTVTAAVAAYEVLSKQFTVPIT
                     IKWVNDLYCERKKVVGILAEMEMTANNQPRGIVVGWGINLAAPTAPLPPALQLRAGWLG
                     TTSIDDHQRLQDDHQRLQIVDAVAAHFLDLLANASWKHILQVYQAHQYLINKLLLVDTG
                     AERVRGYFDHITDDGWLCVQTDNGPRAFMTGTVRELV"
     CDS             complement(7413..10850)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: HMGL-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1090:acetyl-CoA carboxylase biotin carboxylase (Score:
                     92; E-value: 5.7e-28)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg32_9"
                     /sec_met_domain="ATP-grasp (E-value: 9.1e-12, bitscore:
                     34.5, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 2.2e-11,
                     bitscore: 33.3, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="HMGL-like (E-value: 1.2e-36, bitscore:
                     116.3, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKKVLVANRGEIATRIIRACHELGIKAVAVYAKEDEYSAHRFAA
                     DEAYQIGAGEHPLDAYLDIDEVIRVAKESGAEAVHPGYGFLAENADLAQACIDNGLKFI
                     GPKPEHLRMFGDKLVAKRVAVEAGLQPIPGLSGDVKNVDQVKDFAHQYGYPIMVKAANG
                     GGGRGMRIINDDQQLTDEFDQAKDEALKAFGSDEMYVEKDVQHPKHIEVQVIADEHGHV
                     MHLFERDCSVQRRHQKVIEFALSVVLTPERRVEVCGLAVKLMKSVHYQNAATIEFLATP
                     DHFYFIEVNPRVQVEHTVTELITGIDIVKTQIRVAAGEDLHQDIGLPQQDDLKFHGAAI
                     QCRVTTEDPENNFMPDTGQVTTYDAPGGTGVRLDGNVYPGYKVTPYFDSLLVKCCTRGE
                     DFSDARIKMLRALEEFKIRGVKTNIPFMINVLKHPTFIAGKCSTTFIDENPDLFSFAHR
                     PDTPNQLLQYIGNVTVNGFAGVTKRTKLVLPDMQEPQAGQLTPNQHTTKTVKDILDKQG
                     ADAAMQYVKDQPRVLLTDTTMRDAHQSLFATRMRTHDMLPAMKVYDRAMPNLFSVESWG
                     GATFDVCYRFLGEDPWTRLRLLKKYMPHTLQQMLLRASNAVGYQNYPDNELKRFIEHSA
                     ATGVDVFRIFDSLNWIDQMERSITYVRETGKIAEGTMCFTGDFLSPHEHKYTLDYYMKL
                     AQSLVDAGAQIIGIKDMAGLLKPKAAYELVGSLKAKFDVPVHLHTHDTTGNGVPTYVAA
                     TNAGVDVVDVAMSSMAGTTSQPSLATFYYALEGDKRQPDLNMHNVAVLNRYWSNVRPLY
                     NDFSNGVNAPQPDLYQTEMPGGQYSNLKQQAQSLGINDFSLVEEKYREVNQLLGDIIKV
                     TPSSKVVGDMAIFMIQNHLDKDNILTKGQRVDFPESVVNFFAGDLGQPYRGFPKDLQKV
                     VLKDHPAITVRPGSLAKPVDFAAVKQELAAKINQEPSEDDLQGYIMYPKVFTTYFRNHQ
                     RYGEVMDLDTTTYYQGMRPGEILHVKYGQGKEMIIKLDFVSAPDDDGDRFLFFELDGRI
                     LKIKVKDNSMKDKVVSVSKADPDKSNEIGMPLNGNVVKVNVAEGDQVKVGDVLVVTEAM
                     KMESAVKAPFTGKVTKVYAKVGDALKSQDLLLTLTEDA"
     CDS             11551..11730
                     /locus_tag="ctg32_10"
                     /transl_table=11
                     /translation="MTRKVAVVGMGHVGATVAHYLVAGGFTDDLALYDTNEAKVRADAL
                     DLRDAMANLPFHTT"
     CDS             11799..12467
                     /locus_tag="ctg32_11"
                     /transl_table=11
                     /translation="MDTPDHDRFAEFKFTRTQVPLVAKKLVDNGFHGKLVDVTNPCDVI
                     TSMYQKLTGLPKNHVMGTGTLLDSARMRACVGEALHVDSRSVVGFNLGEHGNSQFTAWS
                     TVRVLGKRVLDLVDERHLDLTDMEERARQGGYMVYKGKKYTNYGVATAAVRLTNALLSD
                     ARTEMPVSNYREEYGTYLSYPAVVGRDGVVEQLHLDLTAAEKEKLATSAKYIKDRLGLE
                     "
     CDS             12535..12741
                     /locus_tag="ctg32_12"
                     /transl_table=11
                     /translation="MTKHSYDKEFKEQAVQYYLDNKDHMTMNEISKNLGIGASTLHKWI
                     KLFTETGEFGRGSGNFASDKDKEI"
ORIGIN
        1 gcagaccgag aatggacatg cccaaagtgc catgctcacc acgttcgtga ccataacgct
       61 gcgcagaata ttctaactaa aggaattatt aaattagcct aattcggctt gtccctatgg
      121 caacctgggg acgctaaagg cattggtaat tgccgtgtaa gacgaaacgg ttcgtagaac
      181 tattcgcagt ggtgtatcta tgcaaattgt gattcaaaca ctcatcagaa gggtgcttgc
      241 tcacaagcta ctgactttag tcagtggtgg ttgactttgc gtatgttaga tagaaaacgt
      301 ggtcaataac cacaggccta gtgtctaagc cggccgaaga acgatgcgtt ccgcccctag
      361 cttagtcata cgacctgcca agcaagttat ttgccactcc cttttaatta gtcgatatcg
      421 ttgcaaatta cgaccggctt gattaagtcc gccgacttgt ggtgcatcag ttcgatggcg
      481 tccggaatct tctcgaagct tcgaagcgat gggtgatcat tagtgatgga tcgatccggc
      541 cgttcaccac gaggacggcc agcttttcca tccgcaggcg gccaccaggc atcaggccac
      601 cgtggatggt cttgtgaccc atcccaacac cccagtcacc ggcgttgaag accacgtcgt
      661 cgtgaccgtt taagtagtta acgttcccga ttgagccacc cggcttgagc atccggacgg
      721 cttcttcaaa ggtgtccttg gcgttcccac cggcgatcag gaccttgtcg accccttggc
      781 caccagtggc ttccaggatt tggtcggaaa tccggccgtt gtggtagtca atgatgtcgg
      841 ttgcgccgta ctttttagcc acttcgaccg tcttttcccg tgaaccaacc gcaaagacgc
      901 ggccagcccc gtggagcacc gccccgcgca gggccatcaa gccaaccggg ccgatcccaa
      961 tcacggcgac ggcatcccca aaggtcacac ccgccatttc ggaagcgtgg aggccagttg
     1021 ggatcatgtc ggaaagcatg caggcctcgg tcgggtcaat ttcatccggt aggtgagcta
     1081 ggttcccgtc ggcgtcgtta acgtggaaac gagtggcgaa gaccccgtcc ttgatattag
     1141 agaacttcca gccgcccagc atcccgtcgg aatcagtggc gtagccagct tgggagtaaa
     1201 cgtcagacca gtttggcgtg atcgccggaa cgagtacccg gtcacccggt ttaaagtcct
     1261 tgacgtcttc accgacttcc acaaccaccc cgcaggcttc gtggccgaga attatattct
     1321 cccgtggccc taaggcatct tcaaacacgg tgtggatgtc agaagtacac ggggcaacgg
     1381 ctaggggacg taccaggcgt cacggctgcc caccttggga tcttcttttt caataaagcc
     1441 cgtcttgttg gggcccagca ttgcaaaacc tttcattcat aactaccacc tttcttgatt
     1501 agttgccgtc attctaacat aaatcatgtt ataaccaagt cttcatatgt gagtttaatt
     1561 aattttaaag aaagcgttct cattccctga aagggttaaa ataacaggga ttatgatagt
     1621 taagaaattg attggaaata aataaaatga ctgtgataat cccgtacgag gtcaccacag
     1681 tcattaatca ttctataatt agcggattat ttagctttta aattcccaac gaagagggac
     1741 gccaccccgg ccaccaaggc ggagatgacc aggccgaaga aggccgtgtc gtatgaaccg
     1801 ctcagctgaa cgatccagcc gattaagagg ggaccgaaga agcccccgag ttggccacca
     1861 aagttaatcg tcccgatctg ggagccataa gtcttttgcg ggaagagttg ggccgcgtag
     1921 gagaagatcc cggtgaaggc tagcgacttg acgaagtagg tggccatttg gaagatcact
     1981 aaccaaacca ggctggtgga cttgtagagt ccaaacatgg tcaggaccgt taaaaaggag
     2041 gccaggataa tcatcaggcg ttcgtgattt ttaaagatgt gcaccattac gtaaccggaa
     2101 atcaaggccg caatcccccc ggcaatcgtc ggtaggggaa ctaaccaagc caagttggct
     2161 aagttgatgt ggcgggcgtt taagaagtaa accggcatcc aggtttccat ccccttggtg
     2221 ataatgttta aggccagccc gatcaccacg aaggcccaga cccgcgggtc gatttccttc
     2281 cagtttatct tttgccgttc ttgtttcgtt accgtttgtc caccgccgtg accgcggaag
     2341 aagaggtagc tgaccatcaa gagtaggcca aagcccccca accagaagaa ggccgagcgc
     2401 caaccggtag cggcgatgat tggcacgatg atgaaagggg ccagggccgc gccggcgtag
     2461 ttagaggaaa ttaaggcggc ggtcagttgg gcacgcttgt tgaccgggaa gtcctgggcg
     2521 acttgcttca tggcggccgg tgggtaaggg ccctcggcga tcccaaacag gaaccgaatt
     2581 accaagaggg cggctaagga ccaggccagc ccggtaaaga tcgtcatcac cgaccaaaag
     2641 gcgagggtgg cgatgatcac cttacgggaa gagaagcggt cggtcagcca gccccccggg
     2701 atttgcatga tcgtgtagcc caagaagaag acggaactag tcgcccccag tgcggccggg
     2761 ctaacggaga aatccttgcc gatcgcggcc aacgataagt taagcacggt ccggtcggcg
     2821 aataagagca tgtaaccaag gtataaaatc acaaaggaac cgagcgacag cgcccggcgg
     2881 ttgggttttg catccatcat ttatttgtat ctttcttttc tcaattgatt attctgttgt
     2941 ggcatgacta agcgttagcg accaggtctt gtaagcgaaa cttttggacc ttgccgctgg
     3001 cgtttttcgg tagggcgtct gtttgcaagt agaccttggg taccttatag tgggccaggt
     3061 gttcacgccc gaaggcctcc aactgctgaa agtcgacggg ttgtttgctg acgaccacgg
     3121 ccacgggaac ggcgccccac tgttgatcat tacggccgac cacggcaatt tcttggatgc
     3181 ctgggtactg gcgcaagacg ttttccacct cggcggggaa aatgttttcg cccccggaga
     3241 tgatcatttc atctaaccgt ccgtcgacgt acaagaaacc gtcttcatca aagtggccga
     3301 cgtcgccggt tcggtaccag tcgccatcgt gcttagccgc aaacttggcc ggttggttta
     3361 agtaacccgg ggttaaggcc ggggtctgga tctcgatttc gtgatgctca cccaccaaac
     3421 gcagctgggt ggtaaacagg ggcttaccga ccgaaccaag gtggtaaggc gcctcttcag
     3481 ccgagagggc aacgaactgc gagcaagttt cggtcatccc gtaacactgg acaaccggca
     3541 gcccactttc ttcgcaggcg ttaagggtcg cccggtcaat cgtcccaccg ccgagtagca
     3601 ccaggcgaaa ggccgatgag taacagtggt catttgcttg gacgtcttcg agcatcttct
     3661 ttagcatgta gggaacggcc gaaatgatcg taaccggttc gtcctccagg atttggttga
     3721 ccacttgcgc atcgaaatgg ttgactaggc gaacgcccat cccgtaaatc aggccccgca
     3781 ttagaattga aaagcccgaa atgtggaaga tgggggcgac gcacaaccag aggtcgtttg
     3841 gcaacacccc gaggttcaaa gatgacgaaa tggcggaggc caagtggttg ccgtaggttt
     3901 gcaagacgcc ctttggctga ccggtggttc ccgaggtgta catgatcgag gccacggcgt
     3961 ctaggtcaac cgtcggcgtc aacactgccg ccggggcagt gccggctgtc aattccgaac
     4021 agaggacccg gtgcccaccg taatagcggt cggctagttg gtcatcgact aagactaggt
     4081 cgagccccgc gtcgccgatt tggcggctca gttcttcagg ggcaaggcgc caatttagcc
     4141 aaacgatggt cttgtcggcg gctaagaccc ccagggcggc taagtaacca gccaggttat
     4201 tgtgggtcat caccccaacc cggggctggt ccgtctgcgc ttgaatctgg ccagcacggt
     4261 gggcgaccag gggaaccagc tgggcaaagg tgtaggcggt ggtaccgtcg ttaaccgcca
     4321 cccggttagg gtttagcgaa gcctgtttga ggagccaatt tggaatctcc atgacggcct
     4381 cctacgggaa cttcgggaac ttatcgaagt cggggtcgcg cttttggtta aaggcgtcgc
     4441 gcccttcctt tccttcgtca gtcgtgtagt aaagcatcgt ggcgtcaccg gcaaattgtt
     4501 gaagccccgc taaaccatct gtatcggcgt tcatggccgc cttaatgaag cgaagggcgg
     4561 tcggtgactt ctttaagatt tcatgacacc agtctaaagt aactgattcg acctcagcta
     4621 ggggcgccac cttgttaatc cagttcattt ggtaagcttc gtcagccgtg tagaagtggt
     4681 ttaagaacca aacttccttg gcccgcttgt ggccgatcac ccgggctagg taacccgagc
     4741 cataaccggc gtcaaaggaa cccaccttcg gccccgtttg cccaaacatg gcgttatcgg
     4801 cggcgatcgt caggtcacaa accagttgta agacgttccc gcccccgact gaccagccct
     4861 tgaccatggc aatcaccggc ttggggatga tccgaatcaa gtgttggagg tccaaaacgt
     4921 tgagccgggc gatgtggtcg gggcccacgt aacccccgtt accccgcacg gattggtcac
     4981 caccggaaga aaaggcctta tcgccggctc cggtaagaat aatcacgcca attgtgctat
     5041 catcacgaca gatgttaaac gcgtcgatca tttcggcgac cgttaccggg gtgaaggcat
     5101 tgtgcttttc cggccggttc attgtaatct tggcaatctt atcgtcgcgt tcaaaaataa
     5161 tttcggaata ctccttaacg ggggtccagt tagcactcgt cataacaaac tcctttacaa
     5221 aatccagagg tacaaaaatg aatttacttg aaaacttagg cattcaccag gacttggtga
     5281 ccaaagaccg cgttgaactc tccatgccgg tccaagcaaa gcacttgcaa ccgtacggga
     5341 tcgttcacgg cggaattaac accgtgctgg cggaaaccgc ggcctcccta ggggctaacg
     5401 ccaacgtagc gccggggcga atcgcggtcg gggtcaatgt taccacacaa catatccgtg
     5461 cgatacggga aggggagatt tttgtggttg caacccctga tcacgttggt cgcacacttc
     5521 agacctggca agcccggcta ctggttaacg accgattaac ggctaccgca accatcaccc
     5581 tgatgaacgt cccagcacca accaacaatc ataaacatta attcacaaat tgtctactat
     5641 tatgcttaga aagttgccgg gtgaccattg ggggattacc taaatcacat tttttaattt
     5701 tatattatgt aaatcaatcg agaagtcgtc ggctaactgt tatgaaaggg gcgccactac
     5761 gcagtcgatt taagttggcc ccgattttgt attagtgttg caaccagtac gcaaaagcac
     5821 acataattta tcgaggggag gaccagatat gaggaaatgg tgggtggaat tgaaactggt
     5881 cgtcttttgc atcgccgctc ttttcaggta attactctaa cggcgctaat tagacttact
     5941 tctgtaaatt tacattatgt aaagatatcg tttgatgatt aaagaagcct caaaggccct
     6001 ccgaggatga caatacagat gttcaagact taattacgta cgctctcact gagttcctga
     6061 acaatatggt atttaacgac attcgcaatt cgacaggcaa aattaactcc ttgaatctcg
     6121 gcaaccttgg taactcgctt ggggcattgg ggcgatggtg gacagagata ctgttgtttc
     6181 ttgattttta agacagctgg ttttccagcc ggtgtaagga ttttgatcgt tacgggccgc
     6241 ttacggaagc cattacgacg catcaactgg ccgcaaagcg gacagtgcat gggataagac
     6301 tgaattaaat ggataatctt tagttcttta tcttcagtta aaaatggttt gtcaggtttt
     6361 aagtaaggat ccttgattcc agtccaaaaa ttgatatcat tagtcatgag gaatatatcc
     6421 tttctaagac aactgagtgg ttatggggat aacttaagac agttgtcttt tttattttta
     6481 aatcaatggt acagcaagta acaaaaaacg gtaaagacaa gaaatcatct tatccttacc
     6541 gaatatcata gaaccttcaa ggataactgc ggtcaaaaca cgtccgcgtt tgtccaagaa
     6601 ggactgcttt tttaattact aaaccagttc ccgaaccgtc cccgtcatga atgcgcgggg
     6661 cccgttatct gtctgcacac ataaccagcc atcatccgta atatggtcaa agtatcccct
     6721 aacacgttcc gctcctgtat caacgaggag cagcttgtta ataaggtatt gatgagcctg
     6781 gtaaacctgc aggatgtgtt tccaggacgc gtttgctaac aggtcaagga agtgcgcagc
     6841 gactgcatca acgatttgta gccgttgatg gtcatcttgt agccgttgat ggtcatcaat
     6901 tgaggtggtg ccgagccatc ccgcacgtaa ctgaagtgct ggcggcaagg gagcagttgg
     6961 tgccgccagg ttaatccccc agccgaccac tattccccgc ggctgattat tcgcggtcat
     7021 ttccatttca gcaagaatcc ccaccacctt ctttcgctca cagtacaggt cgttcaccca
     7081 tttaatagta atgggaacag tgaattgctt gctgaggact tcataagcag cgaccgcggc
     7141 agtcaccgtt acctgaccag cactttttgc caggtagcta aagggtaagg caagggtgaa
     7201 gtaggcccca cttcccgcgg gtgaataaaa cttccggctg gtctttccgt ggcctgccgt
     7261 ttgctgccgg gcggagatga gaaagggctg atctttcacc tttaactgtt cctttgccat
     7321 ttcgttagtc gaagtgacca ctggctgatc ggttgtctgg atatgatgtg ctgtcagtct
     7381 aggaaattcg tacatcattg ctactctcct tatcaagcat cctcagtcaa tgttagtaag
     7441 aggtcctgac tctttaacgc atcgccaacc ttagcgtata ctttcgtcac cttaccagta
     7501 aatggcgctt taactgctga ttccatcttc atcgcctcag taacaaccag aacgtcacca
     7561 accttgacct ggtcaccttc tgctacgtta accttgacca cgttcccgtt caacggcatc
     7621 ccgatctcat tactcttatc gggatcagcc tttgaaacac tgacaacctt gtccttcatg
     7681 ctattgtcct taacttttat tttaaggatc cgcccgtcca gttcaaagaa gaggaagcga
     7741 tcaccgtcat catctggtgc tgaaacaaag tcgagtttta taatcatttc ttttccctga
     7801 ccatacttaa catgcaaaat ttcaccagga cgcatacctt gatagtaggt cgtggtatca
     7861 aggtccatca cttcaccgta ccgctgatgg ttgcggaagt aagtggtaaa gactttcgga
     7921 tacatgatgt aaccctgcag gtcgtcctcg ctcggttcct ggttgatctt tgcggccagt
     7981 tcctgcttaa cggcggcaaa atcaaccggt ttagcaaggc tcccgggccg aaccgtgatt
     8041 gcaggatggt ccttcaagac aaccttctgt aggtccttgg ggaagcccct atatggttga
     8101 ccaaggtcgc cagcaaagaa gttaaccact gattctggaa agtcgactcg ttgccccttt
     8161 gtcaggatat tgtctttatc aaggtggttt tggatcatga agattgccat gtcgccaacc
     8221 accttcgagc ttggcgtcac cttgatgatg tcaccgagca actgattgac ctcccggtat
     8281 ttttcttcca ccaggctaaa atcattgatt cctagtgact gggcctgttg cttcaggttc
     8341 gagtactggc cacctggcat ttccgtctgg tataggtctg gttgcggtgc gttgaccccg
     8401 ttgctgaagt cgttgtaaag cggccggaca ttggaccagt accggtttaa gaccgccacg
     8461 ttatgcatat taaggtccgg ttggcgttta tctccttcta gcgcgtagta aaaggtggct
     8521 aaactaggct gactggttgt ccccgccatt gaagacatcg ccacgtccac cacgtcaaca
     8581 cccgcatttg tggcggcaac gtaggttgga actccattac ccgttgtgtc atgagtgtgg
     8641 aggtgaacag gaacatcaaa cttagccttc aaactaccga ctagttcgta agcggccttt
     8701 ggtttcaaga ggccggccat atccttaata ccaataatct gcgctcccgc gtcgactaat
     8761 gactgtgcca acttcatgta ataatctagg gtgtacttgt gctcgtgagg actaaggaag
     8821 tcgccggtaa agcacattgt tccttcggcg attttgccgg tttcccgaac atatgtaatg
     8881 ctacgttcca tctggtcaat ccagttgagg gagtcaaaga tccggaaaac atccaccccg
     8941 gttgcggcac tgtgctcaat gaaccgcttc agttcattat ccggataatt ctggtaaccg
     9001 accgcgttcg acgcccgcaa caacatctgc tggagagtat gtggcatgta cttcttcaat
     9061 aatcgtaatc gggtccacgg gtcttcacca aggaaccggt agcacacgtc aaaggttgcc
     9121 ccgccccagc tttcaacaga aaagaggtta ggcatcgccc ggtcgtaaac cttcattgcg
     9181 ggcaacatat cgtgcgtccg catccgagtg gcaaaaaggc tttggtgagc atcacgcatt
     9241 gtggtatcag tcagtagtac cctcggctgg tccttaacat actgcatcgc cgcatcggct
     9301 ccctgtttat ccaggatgtc ctttactgtt tttgttgtat gctggttagg tgtaagttgg
     9361 cctgcttgtg gttcttgcat gtcaggcaaa acaagtttgg tccgcttggt tacccctgca
     9421 aagccattga cggtaacatt gccaatatat tggagcaact ggttaggcgt atcgggccgg
     9481 tgcgcaaagc taaagaggtc gggattctcg tcgataaacg ttgtcgagca ttttccggca
     9541 ataaacgtgg gatgctttaa cacgttgatc atgaagggaa tattagtttt gactccccga
     9601 atcttgaatt cttcaagcgc ccgcagcatc ttaatccggg catcactgaa gtcttcaccc
     9661 cgggtacaac acttaacgag caaggaatca aagtaaggag tgaccttata gccgggatag
     9721 acgttcccgt caagccggac accggttccc cctggcgcat cataggtcgt aacctgtccc
     9781 gtatctggca taaagttatt ctcgggatct tctgttgtga cccggcactg aatggctgcc
     9841 ccgtggaact tgagatcgtc ttgctgggga aggccaatat cttgatgaag atcttcaccg
     9901 gccgcaaccc gaatctgggt cttaacaata tcaatcccag taatcaactc ggtaacggtg
     9961 tgttcaacct gcacccgggg attaacctca atgaagtaaa aatgatccgg ggtcgccaag
    10021 aattcgatcg tcgccgcatt ctggtagtga acactcttca tcagcttgac ggccaggcca
    10081 caaacttcga cccgccgctc aggggtcaag acaacgctaa gtgcaaattc aataaccttc
    10141 tggtggcggc gctggacaga gcagtcacgt tcaaagaggt gcatcacatg cccgtgctca
    10201 tcagcaatta cctgcacttc aatatgcttc gggtgttgga cgtccttttc gacatacatc
    10261 tcatccgatc caaacgcctt cagcgcctca tccttcgcct gatcaaactc gtcggtgagt
    10321 tgctggtcgt cattgatgat ccgcatcccc cgaccgccac caccattagc agccttaacc
    10381 atgatcgggt aaccatattg gtgagcaaaa tcctttacct ggtcaacatt tttaacatcc
    10441 cccgataaac cgggaattgg ttgcagccct gcctcaacgg ccacacgttt agcaaccaac
    10501 ttatccccaa acatacgaag gtgttccggc ttaggtccaa taaactttaa tccattatca
    10561 atacaggcct gcgcaaggtc agcgttttct gccaggaagc cataaccagg gtgaacagct
    10621 tctgccccgc tttctttggc cacccgaatt acttcatcaa tatccaggta agcatcaagc
    10681 ggatgttcac cagccccgat ttggtacgcc tcatcagcag caaagcggtg cgcactatac
    10741 tcatcctcct tagcataaac tgcgacggct ttgattccca gctcatgaca ggcgcgaata
    10801 atccgtgtag caatttcacc acggttagca actaaaactt ttttaaacac aattatccac
    10861 catccttttt attaatcctt cacgcccatc acgtcattac tattggcggt gagggtggct
    10921 gtatttgtag aaatattaat attaccaacc attgcactca cctcctcatt tttctaatca
    10981 aattttaaaa caaactataa tcaatctaag ctaacgtgtc aataattttt cctcaagaaa
    11041 tgcccgctat caaacgaatt tatactataa attatctgcc tttgcaccca tcttttctca
    11101 tcttttttta atttttatct tgacttccct atctgcctag tgatatagta cttaacaatc
    11161 aattgaaaca agttaaagac gttgagcgag aaacagcgat gaggccgaag caagagagct
    11221 ggtgggtggt gtgaaccagt gagaggccgc acctactaat cactcgtgag tcgtacgccg
    11281 aacggattcc ctagtagagc gtattggtag cacccattac cgtgccggct aataccacat
    11341 tatttgtggc ggttggttga ggcgacatta tgtcgtaaag aaaggtggta ccacgcacaa
    11401 gcgtcctttt tagttcttag atttatcaag agctagaaag gacgcttttt gttttgatat
    11461 taaaggtggt ggtgatcatg gcagacagtg atatttctga cattagtaag attaaaggca
    11521 acaatacgat aatcgaaagg aagttttatt atgacaagga aagtagcagt agttggaatg
    11581 gggcacgttg gcgcaactgt cgcccattat ctcgttgctg gtggattcac tgatgattta
    11641 gccctctatg atactaacga agcaaaggtc cgtgccgatg cgcttgacct ccgtgacgcc
    11701 atggcgaatc tcccgttcca cacaacttaa ccgttaatga tgattcacaa ttagctgact
    11761 gtgacgttgt aatctctact ctgggaaagt ctaagctggt ggacacaccc gatcacgacc
    11821 gctttgcaga atttaaattt acccggactc aggttccatt ggtcgctaag aaactcgtcg
    11881 ataacggttt tcatggcaaa cttgtcgacg ttaccaatcc ctgtgacgtc atcacttcaa
    11941 tgtatcagaa gctcacgggg ctaccaaaga atcacgtgat gggtacgggg accctgctag
    12001 actccgcacg aatgcgcgca tgtgttgggg aagccctcca tgttgactca cgatccgtgg
    12061 tcggcttcaa tcttggtgaa cacggtaact cccaatttac tgcctggtca acagtccggg
    12121 ttctcggcaa acgagtacta gatctggtgg atgaacggca cctcgacctt accgacatgg
    12181 aagaacgcgc acggcaagga ggctacatgg tctataaggg aaagaagtat accaactacg
    12241 gtgttgctac tgccgcagtc cggcttacca atgccctact aagtgatgcc cggactgaaa
    12301 tgccagtttc aaattaccgt gaagaatacg gaacgtacct ctcctacccc gcagtcgttg
    12361 gtcgtgacgg ggtggtggaa caacttcacc tggaccttac cgctgctgaa aaagaaaagc
    12421 tcgctacctc tgccaaatac atcaaggatc gcctgggcct agaataaaaa gtgtacaagt
    12481 taaatagaga ctctgattta gtataattaa ggaagtaaat tggaggatca aataatgacg
    12541 aagcacagtt atgacaagga atttaaggaa caggccgttc agtattactt agataacaag
    12601 gatcacatga ccatgaatga aataagtaag aatctaggta ttggggctag tacattacat
    12661 aaatggatta agctgtttac tgagactggg gagtttggcc gtggctccgg taattttgcc
    12721 agcgataagg acaaggagat t
//
