LOCUS       c00073_gnlPro..         6014 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AGCBBDJJ_73.
ACCESSION   c00073_gnlPro..
VERSION     c00073_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 22:50:45
            Original ID  :: gnl|Prokka|AGCBBDJJ_73
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6014
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6014
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[895:5868]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      896..5868
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..6014
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6014
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             3..200
                     /locus_tag="ctg73_1"
                     /transl_table=11
                     /translation="WHTMPMKPKEMIRLLKKNGFIKISQNGSHVKMKNFKTGKQTTVPL
                     HSKDLKKGLEDAILKQAGLK"
     CDS             246..635
                     /locus_tag="ctg73_2"
                     /transl_table=11
                     /translation="MKKLFYPAVFHIAEEGGYWVTFPDFPECLTQGENMQEAYDMAIDA
                     LGLILTDDINDHKELPEPSNITHVDDGVVVIIPYDYLEYNRKYHNKSIKKTLTIPEWLN
                     NEALRLNLNFSQILQDALLNKIQSR"
     CDS             complement(896..2308)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: HMGL-like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: PYC_OADA"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg73_3"
                     /sec_met_domain="HMGL-like (E-value: 7.8e-44, bitscore:
                     139.0, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="PYC_OADA (E-value: 1e-58, bitscore: 187.2,
                     seeds: 434, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAEIKKKPVKIVETVLRDAHQSLIATRMTTEEMLPIVDKMDQVGY
                     HAVECWGGATFDACLRFLKEDPWERLRKLRDGFKNTKLQMLFRGQNILGYKPYPDDVVE
                     YFVQKSIANGIDVIRIFDCLNDLRNLQTAVDATKKEGGHAQIALSYTIGDAYTLDYWKK
                     TAKNIEEMGADSICIKDMAGLLVPNEATRLVTALKQATDIPIELHTHYTSGVGGMTYLK
                     AVEAGVDIIDTAMSPFAMGTSQPATEVMAETFRGTEFDTGFDQKLLGEIADYFRPIREK
                     YLANGLMSPKVLGVNIKTLMYQVPGGMLSNLVSQLEQQGASDKFYEVLEEVPKVRKDFG
                     EPPLVTPSSQIVGTQAVLNVLTGERYKMITNESKALLHGEYGQTVKPMNKEVQKKAIGD
                     EKPITCRPADLLEPELSKIEAEMSPWKEQDEDVLTYALFPQVATEFFKYRAAQENKIDP
                     AKADVKNGAYPV"
     CDS             complement(2466..3590)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg73_4"
                     /sec_met_domain="OAD_beta (E-value: 6.4e-158, bitscore:
                     514.1, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYVVETMTNLAAQTGFAFLKPGNFIMILVALVFLYLAIAKGYEP
                     LLLVPISFGMLLVNLYPSIMEEGGLLHYFYLLDEWSILPSLIFLGVGALTDFGPLIANP
                     ASFLLGAAAQFGIYSAYFLAMLMGFNGKAAAAISIIGGADGPTSIFLAGKLGQTDLMGP
                     IAVAAYSYMSLVPIIQPPIMKLLTTEKERQIKMEQLRTVTKTEKIMFPIVVTVVVCLIL
                     PTTAPLVGMLMLGNLFRESGVVKQLSETASNALMYIVVIILGTSVGATTSAEAFLNVST
                     IKIVVLGLVAFAVGTAAGVLFGKLMCKVTGGKVNPLIGSAGVSAVPMAARVSQKVGAEA
                     DPSNFLLMHAMGPNVAGVIGTAVAAGTFMAIFGV"
     CDS             complement(3624..3992)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 66.6; E-value:
                     4.3e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg73_5"
                     /sec_met_domain="Biotin_lipoyl (E-value: 4.8e-22, bitscore:
                     66.6, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNYRITVNGVAYDVAVEELGAGEASAAPVSTPTAVPAPAKKAAP
                     KTSGSAGAVKITAPMPGKIVAVKAQAGASVKKGDAVLVLEAMKMENEICAAQDGVIASV
                     EVAVGDMVEGGDVLATMN"
     CDS             complement(4087..4431)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg73_6"
                     /sec_met_domain="OAD_gamma (E-value: 3.2e-28, bitscore:
                     87.2, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVFEQALLNTLMGMGTVFAVLIFISLLISLFVYIPSIERALKNR
                     SSKKEKKAAQEERPAPKRPILEEVVEEEELVDDGELVAVITAAIMAANGGAAVSADKLV
                     VRSIKRVKRR"
     CDS             complement(4444..5868)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg73_7"
                     /sec_met_domain="CT_Vparvula (E-value: 5.3e-138, bitscore:
                     449.5, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGNSKMNARERIDYLFDDHSFVEVGGYVSARNTDYNLGAKKVEGD
                     GVITGYGVINDRMVYVYSQDASALGGSVGEMHAKKIASLYDLAMKTGAPVIGMLDCAGL
                     RLQEATDALHAFGTIFNKQAQASGMIPQISAVFGMCGGGSAVMSAMSDFTLMEKENGSL
                     FINSPNALAGNEESRQNTAGAQFQSEQAGNVDFVCETEEGLLDQLRILVDVLPSDYEAE
                     DVYFDNDDDLNRVIPELNDAAYDAAYILKSISDNNMYIETKAAYAKEMVTAFIRLNGET
                     VGAVANQPVDGESVLTRNGLEKAERFIKFCDAFSIPLLTVTNVTGLRANAHEERKQALA
                     LAKFTTTLAQVSVPRINLIAGEAYGTAAEVMNSKAIGADFVLAWPQASVGMMDAEQAVR
                     IMYAEEINTDKNKTALIAEKTAEYAEGQSSALAAAKRGYIDDIIEPDATRKRLIAAYEM
                     LYGKKVTPVTKKHTAV"
ORIGIN
        1 ggtggcatac tatgccaatg aaaccaaaag aaatgataag attattaaaa aagaatggtt
       61 tcataaaaat aagtcaaaat ggttcccatg tgaaaatgaa gaattttaaa actggcaaac
      121 aaacaacagt tcctcttcat agcaaagatt taaaaaaagg actagaagat gccatcctaa
      181 aacaagcggg cttaaaatag ccttatttca tctataataa ctcacataca ttggaggtat
      241 ccattatgaa aaaattattt tatcctgctg tatttcatat tgcagaagaa ggcggttatt
      301 gggtgacttt tcctgacttt cccgaatgtc taactcaggg agaaaatatg caagaagcct
      361 atgatatggc tatagatgct ttaggactta tcttaactga tgacattaac gaccataaag
      421 aacttccaga accgtctaac atcacacatg ttgatgatgg agttgtcgta atcatcccat
      481 atgattattt ggaatataat cgaaagtatc ataataaatc tatcaaaaaa actttaacaa
      541 tccctgaatg gttgaataac gaagcattgc gattgaattt aaacttttca cagattttgc
      601 aggatgctct tttaaacaaa attcaatctc gttaatggct ataattcttt catttggtaa
      661 tactgcaatc atagattata atatacttaa caaggcagct aatacgatag cttcacctat
      721 ccatttccgg caagtttagg ttaaaaaaca gaacccccac aactttatag atagcaaatg
      781 tgatgtcgga gcaagtaaaa atgcggctga gcattttcta cgcagcgaaa tcttcacatt
      841 tgctatctat aaagttgcgg gggttcgtct gtttttaatc taaatcaatc caatcctaca
      901 ccggatatgc tccattcttt acatcggctt ttgcaggatc aatcttattc tcctgtgccg
      961 ctctgtattt aaagaattcg gttgctacct gtgggaacaa tgcgtatgta agcacatctt
     1021 cgtcctgttc tttccacggg ctcatctctg cctcgatctt agacagttct ggttcaagaa
     1081 ggtcggctgg acgacaggta attggctttt catcgccgat tgcctttttc tgtacttctt
     1141 tattcattgg ttttacggtc tgaccgtatt ctccatgaag aagggctttt gactcgtttg
     1201 taatcatttt ataacgctct ccggtaagta catttaatac agcctgtgtt ccgacaatct
     1261 ggctggatgg tgtaactaat ggtggctcac cgaagtcttt acgtactttt ggaacttcct
     1321 ctaatacttc atagaactta tcggatgctc cctgctgctc taactgagat acaaggttag
     1381 aaagcattcc acctggaacc tgatacatta acgtcttgat atttacgcca agtacctttg
     1441 gactcattaa accatttgcc agatatttct cacgtatcgg acggaagtaa tctgcgattt
     1501 cacctaaaag cttctggtca aatcctgtgt caaattctgt accacggaat gtctccgcca
     1561 taacttctgt tgccggctgg cttgttccca ttgcaaatgg agacatggct gtatcgataa
     1621 tatctactcc tgcctctact gcctttaaat aagtcattcc gccgacacca cttgtatagt
     1681 gtgtatgaag ctcgatagga atgtctgttg cctgctttaa tgcagttaca agacgcgttg
     1741 cctcattcgg tactaaaagt cctgccatat ctttgataca gatagaatct gctcccatct
     1801 cttcaatatt cttagcagtc tttttccagt agtctaatgt atatgcatca ccgattgtat
     1861 aggaaagggc gatctgcgcg tgtcctccct ctttctttgt tgcatctaca gcagtctgta
     1921 agtttctaag gtcatttaaa cagtcaaaaa tacgaataac atcaattccg tttgcaatag
     1981 atttctgtac gaaatattct acaacatcgt ctggatatgg cttatatcct aagatgttct
     2041 gtccacggaa taacatctgt aactttgtat tcttaaagcc atctcttaat ttacgaagtc
     2101 tctcccatgg gtcttccttt aagaaacgca ggcaggcatc aaatgttgca cctccccagc
     2161 attctactgc atgataacca acctgatcca tcttatctac gattggcagc atctcctctg
     2221 ttgtcattct tgtagcaatc agagactgat gtgcgtcacg gagaacggtt tctactattt
     2281 taaccggttt tttcttaatt tcggccataa tatctccttt tatttcaaat cacgtaacta
     2341 tttgcgccta caactgttat tatctaaaat tcaacttgta aactatttat aaatatcccc
     2401 tgtaacattt tttacagatt atagccctat aaataatgaa ttttaaataa tcaaataatt
     2461 ctaaattata ctccaaaaat cgccataaat gttccggctg ctaccgctgt accgataact
     2521 ccggcaacgt ttggtcccat cgcatgcatc agtaagaagt ttgatggatc tgcttccgca
     2581 ccaactttct gggatacacg ggctgccata ggaacggcag ataccccggc agaaccaatt
     2641 aatggattta ccttaccacc tgtaacctta cacataagtt taccaaacag gacacctgct
     2701 gcagtaccaa cagcaaaagc tacaagacct aatactacaa tcttgattgt gctgacattt
     2761 aagaatgcct ctgcacttgt tgtcgcacca acggatgtac caagaataat aactacgatg
     2821 tacataagag cgttagacgc tgtctctgaa agctgtttta ctacgccgga ctctctaaaa
     2881 aggttaccga gcatcagcat accaactaat ggtgctgttg taggaagaat cagacataca
     2941 acaactgtta caacgatagg gaacataatt ttctctgttt ttgtaacggt tcttaactgt
     3001 tccatcttaa tctgacgttc cttttctgta gtaaggagct tcatgattgg tggctgaata
     3061 attggtacaa gtgacatata agaatatgcc gctaccgcaa ttggtcccat taagtctgtc
     3121 tgcccaagct ttccggcaag gaaaatagaa gtaggaccat cggctccacc aataatggaa
     3181 attgctgctg ctgcctttcc gttaaatccc ataagcattg caaggaaata cgcgctgtaa
     3241 ataccaaact gcgccgcagc tcccagcaag aaacttgctg gattggcaat cagcggaccg
     3301 aagtctgtta gtgctccaac gcctaagaaa atcagtgaag ggagaatact ccattcatct
     3361 aataagtaaa aataatgtaa taatccgcct tcttccataa tggacggata taagttaacc
     3421 agcagcatac caaaagaaat cggcactaac aaaagtggct catatccttt ggcgatggcc
     3481 agatataaga agactaaagc aaccagtatc ataatgaagt tacctggctt taagaatgca
     3541 aatccggtct gtgctgcgag attcgtcatt gtctctacaa cataactcat aaatcttcct
     3601 ccgttttatt ttgcaaatat gctctagttc attgtagcca gtacatctcc gccttctacc
     3661 atatctccaa ctgctacttc cacacttgcg ataacaccat cctgtgctgc acagatttca
     3721 ttctccatct tcatagcctc taatacaaga acagcgtcgc ccttctttac agaagcacct
     3781 gcctgtgcct ttactgctac gatcttacct ggcattggtg ctgtaatctt tacggcaccg
     3841 gcacttcctg aagtctttgg ggcagctttc ttagctggag ccggaacggc tgttggtgta
     3901 cttaccggcg ctgcacttgc ttctcctgca cctaattctt ctactgctac atcataagca
     3961 acaccattaa ctgtaattct ataatttttc attgtaaatc ctcctgaatg attttatgta
     4021 cttctaaaat ctttctatag ctaacttttc aaaactttat aaaaagattc caagaataca
     4081 tttttgctat cttctcttca ctctcttgat tgaacgaacg accaacttat ctgcactgac
     4141 tgctgctcct ccattcgctg ccatgattgc cgctgtgatt actgcgacaa gttcaccatc
     4201 atccacaagt tcctcttctt ccacaacttc ttcaagaatc ggacgctttg gagccggtct
     4261 ttcttcctgc gcagcctttt tctctttctt agaagaacga ttctttaacg ctctttcaat
     4321 agatgggata tatacaaaaa gagaaatcag taaagaaatg aaaatcagta ctgcaaatac
     4381 agttcccata cccatcaaag tatttaataa ggcttgttca aatacgttca tactctcacc
     4441 tcactatact gccgtatgct tctttgtcac tggagttact ttctttccgt aaagcatttc
     4501 ataagcagcg ataagacgct ttcttgttgc gtccggctcg atgatgtcat ctatatatcc
     4561 tctctttgca gctgctaatg cactgctctg tccttctgca tattccgcag ttttctctgc
     4621 gataagtgct gtcttattct tatctgtatt gatttcctct gcatacatga tacgaactgc
     4681 ctgttctgca tccatcattc ctacagatgc ctgtggccat gccagaacaa agtcagctcc
     4741 gatagcttta gaattcatta cttccgctgc agttccataa gcctcgcctg caatgagatt
     4801 aatacgaggt acactaacct gtgcaagtgt tgttgtaaac ttcgctaacg cgagcgcctg
     4861 ctttctctct tcatgtgcgt tggcacgaag tcctgtcacg ttcgttacag taagaagagg
     4921 aatcgaaaat gcatcacaga acttaataaa tctttctgct ttttcaagac catttcttgt
     4981 taaaacggac tcaccatcta ctggctggtt agcaaccgca cctactgtct ctccattcag
     5041 acggataaat gccgtcacca tctctttggc ataggctgcc tttgtctcta tgtacatatt
     5101 gttgtcagaa atagatttta aaatgtaagc ggcatcatat gcggcatcat ttaactcagg
     5161 aataacccta tttaaatcat catcgttatc aaagtaaaca tcttctgctt cataatcaga
     5221 aggaagtaca tcaacgagaa tacgaagctg gtcaagaagt ccttcttctg tctcacatac
     5281 aaaatctacg ttaccagcct gctcagactg gaactgtgct cctgccgtat tctgacggga
     5341 ttcttcattc cccgctaatg cattcggact gttgataaag agactgccgt tctccttctc
     5401 catcaatgta aaatcagaca tagcggacat tacggcactt cctcctccac acattccaaa
     5461 tactgcagaa atctgtggaa tcattccaga tgcctgtgcc tgcttattaa aaatcgttcc
     5521 aaatgcgtga agggcatctg tcgcttcctg taatctaagt cctgcacaat ccagcattcc
     5581 aatgactggc gcgcctgttt tcatcgccag atcataaagg gaagcaattt tcttcgcatg
     5641 catctcacct acggaacctc caagtgcgga agcatcctga ctgtaaacat aaaccatgcg
     5701 gtcattaata actccgtatc cagtgataac accatcacct tctactttct ttgcaccgag
     5761 attataatcg gtattacgag cggaaacata tccaccaact tccacaaaac tgtggtcatc
     5821 aaacagataa tcaatgcgct ctctggcatt catttttgaa ttacccatat tctttcctcc
     5881 ataaaaatgc tatattgtat accttgttgc tctttcattc tatgtaataa taacaaatta
     5941 ttatcatgtt agcaaccatt ttctgttagt tatttaacat ttattttatt ttgcccattc
     6001 cactaaacaa aatg
//
