LOCUS       c00105_gnlPro..         4321 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AGCBBDJJ_105.
ACCESSION   c00105_gnlPro..
VERSION     c00105_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 22:50:45
            Original ID  :: gnl|Prokka|AGCBBDJJ_105
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4321
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4321
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[122:3600]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      123..3600
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4321
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4321
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             123..2375
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg105_1"
                     /sec_met_domain="Gly_radical (E-value: 3.8e-43, bitscore:
                     134.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.1e-267, bitscore:
                     876.9, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQKEQWQGFNGRIWREEINLREFIQDNYTPYDGDASFLEGPTEA
                     TDALWGELQKLQKAEREKGGVLDMDTDIVSSLTSHKPGYISESLKEKEQIVGLQTDKPL
                     KRAFMPFGGIRTAEEACTTYGYTPNPEFHKIFTDYHKTHNQAVFDSYTPEMKKARHNKI
                     ITGLPDTYGRGRIVGDYRRVALYGIDLLVADKQKDFANCGDGTMTDDVIRLREEISMQI
                     KALKDMKVMAESYGYDISEPASNAKEAVQWTYFGYLAAIKTQNGAAMSIGRIATFLDIY
                     IKRDMDKGILTEKEAQELIDHMTMKFRMVKFGRIPAYNQLFSGDPVWATLEVGGLGQDG
                     RSMVTKTDYRFLHTLENMGPSPEPNLTVLYSERLPKAFRDYAAHISITTSSIQYENDDV
                     MRPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKTGQQVGPEYKP
                     ITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYEYALMALIDTNVRRTFA
                     TGIAGFSHVVDSLSAIKYAKVKTVRNEEGLVVDYETTGDFPKYGNDDDRADDIAVWLLQ
                     TFMKKLEKFHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQ
                     NGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERINNLVNVMDGYFAQGAHHL
                     NVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYAVKFISLTKEQQLDVIARTCHDRM"
     CDS             2854..3600
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.9;
                     E-value: 2.5e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg105_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.5e-33, bitscore:
                     103.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDKNTMALVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNPDT
                     WKMTKENGAVWRSADELLNQALRYRTYWKNSGGITVSGGEPLLQIDFMLEFFKKAKEKG
                     IHTVIDTAGGPFTREEPFFSKFQELMKYTDLLLVDIKHIDNKCHKVLTGRSNENILDMI
                     RYLSDIKKPIWIRHVLVPERSDKDEYLTRLADFIHSLDNVEKVEILPYHTMGIYKWKEL
                     GLEYPLEGIDPPTKERVENAKKILAI"
     CDS             complement(3780..4319)
                     /locus_tag="ctg105_3"
                     /transl_table=11
                     /translation="IENQIITVKKASYKVSYGTTYNLKKEVKAAGDLTFTSSNKKIISV
                     GSKSGKLIVKKPGKVKIKITAGATADYKQTSRIVTIYAVPKKQTIKKVSTAKRKVKVNI
                     KKDVKATGYQIVAAKNSRFSKGKKVLTKKGTRQVTYTITKLNSRKIYYVKARAYKTIGN
                     KKYFGPWSKVKKIRIK"
ORIGIN
        1 taaacttaaa taagtaaaac taatcacaat gaatcatctg acaattttgt tgattttttt
       61 agcaaacata tttcaaaacc ttgacttaaa gattttttta acaaacaggg aggtcaatta
      121 agatgttaca aaaagaacaa tggcagggat ttaatggacg aatctggaga gaagaaatta
      181 atttaagaga gttcatccag gataactaca ctccatatga tggagatgct tctttccttg
      241 aaggtccaac ggaagctact gacgcactgt ggggcgaact gcagaagctg cagaaagcag
      301 agcgtgaaaa gggtggtgtt cttgatatgg atactgatat cgtatcaagc cttacttccc
      361 ataaaccggg ttacatttct gagtctttaa aagagaaaga acagatcgta ggtcttcaga
      421 cagataaacc tttaaaacgt gcatttatgc catttggtgg tatccgtact gctgaagaag
      481 cttgtacaac ttatggttac acaccaaatc cagaattcca caagattttt acagattatc
      541 ataagacaca taaccaggca gtatttgatt cttatactcc tgaaatgaag aaagctcgtc
      601 acaataagat cattactggt cttccagata cttacggacg tggacgtatc gttggtgact
      661 accgtcgtgt agctctttac ggtattgacc tgttagttgc tgacaagcaa aaagatttcg
      721 caaactgcgg tgacggaaca atgacagatg acgttatccg tttaagagaa gaaatctcca
      781 tgcagattaa agcattaaaa gatatgaaag ttatggctga aagctatggc tatgatattt
      841 ccgaacctgc ttctaacgca aaggaagctg ttcagtggac gtatttcgga taccttgcag
      901 caatcaagac acagaacggt gctgctatga gtatcggacg tatcgctact ttccttgaca
      961 tttatatcaa acgtgatatg gataagggaa tccttacaga gaaagaagcg caggaattaa
     1021 tcgaccatat gactatgaag ttccgtatgg ttaagttcgg acgtatccca gcttacaacc
     1081 agctcttctc cggtgaccct gtatgggcaa cacttgaagt tggcggactt ggacaggacg
     1141 gtcgttccat ggttacaaaa actgactacc gtttcctcca cacattagaa aatatgggac
     1201 catccccaga gccaaacctc acagtacttt actctgagcg tcttccaaaa gcatttagag
     1261 attacgcagc tcatatttct attacaacaa gttcaatcca gtatgaaaat gatgatgtaa
     1321 tgcgtccagt atggggtgat gattactcca tctgctgctg tgtttctgct acacagactg
     1381 gtaaggaaat gcagttcttc ggtgctcgcg ctaaccttgc aaaatgtctc ctttacgcta
     1441 tcaatggtgg tgtggatgaa aagaccggac agcaggttgg accggaatat aaaccgatta
     1501 cttccgaata tcttgactat gatgaagtaa tgcataaata tgatatcatg atggactggt
     1561 tagcaggttt atatgtaaat acattaaatc tcatccagta catgcatgac aaatattact
     1621 atgaatatgc tttgatggcc ttaatcgata caaatgtacg tcgtacattc gctacaggta
     1681 tcgcaggatt ctcacatgta gtagattcct taagtgcgat caaatatgcc aaagtaaaaa
     1741 cagtccgcaa tgaagaggga cttgttgttg attatgagac aactggcgac ttccctaaat
     1801 acggaaatga tgatgaccgc gccgatgata tcgctgtatg gttacttcag acattcatga
     1861 aaaaactgga gaaattccat acttatcgtg attctgaacc tacaacttct atccttacta
     1921 tcacatccaa cgtagtatat ggtaaggcaa caggttccct tccagatgga agaaaagccg
     1981 gtgaaccact ttccccagga gcaaacccaa gttatggtgc agagcagaac ggtctcttag
     2041 cttctttaaa ctctgtagct aaacttcctt acgaatgggc attagatggt atttccaata
     2101 cacaaacaat caacccggat gcactcggtc attccgaaga agaacgtatc aataacttag
     2161 taaatgttat ggacggctac ttcgcacagg gtgctcacca cttaaacgta aatgtatttg
     2221 gtgttgagaa actgattgat gctatggaac atccggaaaa agaagaatat gcaaacttta
     2281 caatccgtgt ttccggttac gcagttaaat tcatctctct gacaaaagag cagcagttag
     2341 atgtaattgc aagaacatgc cacgatagaa tgtagtaaaa ataatttata cagaaagcag
     2401 gatgatgagc ctgaacgatg catagactag agcgtgttta aaaaatgctt tctgtaagat
     2461 atgcgttaca atttgcggga aatttcgcct gaatgagagc ggcgtagcgg gctacgtcaa
     2521 ctgaatgaag gcaaaatata ccgtaaattg ggacgtgcag attacaggaa tgatttttca
     2581 aacacgctct agttagctcc tatcctaatg aagaaaaaag agatactttt atgcaccgta
     2641 ctgaacattc cattttgccc aataaaacaa aatcttcgga gaaaacagtc cgaagatttt
     2701 acggtcaaaa ttccatagta cggaatgata aaagtattct cttttttctt cattaggata
     2761 gtttagtttt tgatatgcac ctatttatat gctactattt gctttctccc tggtaactta
     2821 tttttatact gaaaatatta tgaggtgtta tttatgagtg ataaaaatac gatggctctt
     2881 gttcattcta cagagagttt tggttcggtg gatgggccgg gagttcgttt tattgttttt
     2941 ttacagggtt gtcctttgcg gtgtcagttc tgtcacaatc cggacacctg gaaaatgaca
     3001 aaggaaaatg gtgcggtgtg gagaagtgcg gatgaactgt taaatcaggc actccgttat
     3061 cgtacttact ggaaaaatag cggcggtatt accgtaagtg gtggagagcc tcttttacag
     3121 attgatttta tgcttgagtt ttttaaaaag gcaaaagaaa aaggaatcca caccgttatt
     3181 gatactgccg gaggaccttt tacaagagag gaacctttct tttctaaatt tcaggaattg
     3241 atgaaatata ctgacctgtt attagtggat atcaaacaca ttgataataa atgccacaaa
     3301 gttttaactg gtcgcagcaa cgagaatatt cttgatatga ttcgctatct ttccgatatc
     3361 aaaaaaccaa tttggatccg ccatgtcctt gttccggaac gaagcgataa agatgaatat
     3421 ttgactagat tagctgattt cattcactct cttgataatg tggaaaaggt ggaaatcctg
     3481 ccttatcata cgatgggtat ttataaatgg aaagaacttg gattagaata tcctttagaa
     3541 ggaattgacc ctccaacgaa agaacgagtg gagaacgcca agaaaattct tgctatttga
     3601 tttgccgaca aacagaatcc taaaaacccc catatttgtg actttgaaga tagattgcgt
     3661 tgaatactta ttcatcttca atctatcctt gaagtcacaa tatggggggc tctttagata
     3721 taaacttcag ttaaaattcg ctcaaattaa attctaattt ttcaaatgat ctatggaatt
     3781 tactttattc ttattttctt tactttgctc catggaccaa aatatttctt attgcctatt
     3841 gttttataag cacgtgcctt tacatagtaa attttcctag aatttaattt tgttattgta
     3901 tatgttacct gtcttgttcc tttctttgtt aataccttct tgcctttgct aaatcttgag
     3961 ttcttagctg caacaatctg atatcctgta gcttttacat ctttctttat attcactttt
     4021 acttttctct tagctgtaga aacctttttg atggtctgct ttttcggaac tgcataaata
     4081 gtaactattc tactggtctg tttataatct gctgttgcac cggctgttat tttaatcttt
     4141 acttttcctg gcttctttac aataagcttt ccacttttac ttccaacaga aataatcttt
     4201 ttattacttg aagtaaatgt caaatctcca gctgctttta cttccttttt aagattgtat
     4261 gtcgttccat aagaaacttt atatgaagct ttctttacag taattatctg attctctata
     4321 t
//
