LOCUS       gnlProkkaKNCMLPFH_253   4688 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KNCMLPFH_253.
ACCESSION   gnlProkkaKNCMLPFH_253
VERSION     gnlProkkaKNCMLPFH_253
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 02:08:46
            Original ID  :: gnl|Prokka|KNCMLPFH_253
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4688
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4688
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[2972:3872]"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2973..3872
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     cand_cluster    1..4688
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..4688
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="1"
                     /rules="(Terpene_synth or Terpene_synth_C or phytoene_synt
                     or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps
                     or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /tool="antismash"
     CDS             2..211
                     /locus_tag="ctg253_1"
                     /transl_table=11
                     /translation="MERMPIKINFPVPAFPEGASFFTRNLIMNFQGLRFEKPGNYSIDL
                     TVDGELASRVPFRVVQVQEEAAQA"
     CDS             512..1357
                     /NRPS_PKS="Domain: PKS_KR (11-174). E-value: 1e-13. Score:
                     43.4. Matches aSDomain: nrpspksdomains_ctg253_2_PKS_KR.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     164.2; E-value: 6.5e-50)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg253_2"
                     /sec_met_domain="adh_short (E-value: 8.8e-34, bitscore:
                     105.7, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYTARLQYDRIIITGASSGFGEAFAGTLAPHTAELVLIARNEAAL
                     RQLAAALEKRHPGLHASVLPCDLADETSLNTLISRLDSLPPGRTLLINNAGAGDYGDFA
                     DGRWEKIRSLLRLNVESLTRLCHALIPVMKRNGGDIINLSSLGALLPIPDFAVYAATKA
                     YVSSLSEALRLELREHGIRVMAVCPGPVSTGFGKAARRPGFTGNMMPGRNAFDTSVETV
                     VKDSLRALARGRARVFPGMKIRLAAMLLAAAPLGLIRLFMGRRPRKVLPASNDSPSSAS
                     "
     aSDomain        545..1033
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg253_2_PKS_KR.1"
                     /evalue="1.00E-13"
                     /label="ctg253_2_PKS_KR.1"
                     /locus_tag="ctg253_2"
                     /protein_end="174"
                     /protein_start="11"
                     /score="43.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="IITGASSGFGEAFAGTLAPHTAELVLIARNEAALRQLAAALEKRH
                     PGLHASVLPCDLADETSLNTLISRLDSLPPGRTLLINNAGAGDYGDFADGRWEKIRSLL
                     RLNVESLTRLCHALIPVMKRNGGDIINLSSLGALLPIPDFAVYAATKAYVSSLSEALRL
                     "
     CDS             1354..2406
                     /locus_tag="ctg253_3"
                     /transl_table=11
                     /translation="MKAHRRGIPRLTWFKLAVCLCLAAMAVSLFTPYPAQIIRALKGEP
                     EQAPPDSGTCPPQESPPAEQPGQEEQPITAAPSASGKEPGDERLYTPWTPERTIALPPV
                     QYPPFPPVLPTRPAEGAMTHIYELARGLNLKTQVNFQPGTLASRDRETKSNYQMTLSLN
                     VKQPKALTKKEDILKRNPKLEPMLPGLSTLFRHARVSPYYGQVYVRKQTEIRKNLASLL
                     KLLDRHNYYDTETILETAYPDTGRKLLWLQSEMDVVSDGSDGDRLAAMPDKILKSSFYQ
                     PSTSYRWKKRTDKPNPLLNPWQQRLASYKKTLEXXXXXXXXXXXXXXXKSPRRGKNGSA
                     PQNRPCGTGH"
     CDS             2456..2971
                     /locus_tag="ctg253_4"
                     /transl_table=11
                     /translation="MVPLGVVNQSSPFSPQFGDYAVVIVGDKLYPALVGDAGPRYKTGE
                     GSLRLSREINPKAGPYSRPVSDLKVSYLIFPGSAEPEAGPPDYEKLTDRCRELLNEIGG
                     MGKGFKLHQWEDLLAPKPPPAPKPESGGTAETSADKRKKEEAPGNKPAPAPASPQTAPA
                     SSAAKSAA"
     CDS             complement(2973..3872)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg253_5"
                     /sec_met_domain="phytoene_synt (E-value: 1.2e-13, bitscore:
                     39.5, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKGLDHLLSAVSRSFYLSMRFLPREMRSGVAVGYLLARATDTVAD
                     AVDMDCRERLALLRMMGETVAGAASREVVATCFGRLGVLSTIQTHRGEAELLARFPECL
                     ALLENLSPHERMLVRQVLASIVEGQAWELMDFESHAAVSCPEQLEHYTYMVAGCVGEFW
                     TRLGLLAQGPGFSTQPEEQLLRWGRHYGMGLQLVNILRDREEDLARGRSYLPAPDDRPW
                     LDRAERWLKEGVFYAESLRNGRLRFSTVLPAWLGLDTLELLQRADRRHPSSGKVKIARG
                     DVYFRMWKAFLFSWKEAL"
     CDS             complement(3869..4687)
                     /gene_functions="regulatory (smcogs) SMCOG1112:sigma-54
                     dependent trancsriptional regulator (Score: 352.1; E-value:
                     8.4e-107)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg253_6"
                     /transl_table=11
                     /translation="MNLSGRKDKPLVTLNCAAMPENLLESELFGHEKGSFTGATSRRIG
                     RAEAADGGTLFLDEIGDLSLQMQVKLLRFLQEKTFSRVGSNRELHADVRFIAATSRNLE
                     ELMAANKFREDLYYRLNIFPIVMPDLSKRKGDVMLLAEHFLSKFNLKYGKDIKRLSTPA
                     INMLMAYHWPGNVRELENCMERAVITAQDDCIYGYNLPASLQMPSHDVPYSRDGEAPAD
                     LPTMVDSFERELIVAALKRSPGNMSAAARELGISPRVLHYKMHRLGLQKS"
ORIGIN
        1 caaggaacgt atgcccatca agatcaattt cccggttccc gctttcccgg aaggggcttc
       61 cttcttcacc cgcaacctga tcatgaactt ccagggcctg cgttttgaaa aaccgggcaa
      121 ctactccatc gacctgacgg ttgacggcga gctcgcctcc cgcgtgcctt tccgcgtggt
      181 tcaggttcag gaagaagccg cacaggctta agcaacctcc cctttccatc aaaccgccta
      241 ccgttcatcc ggcaggcggt tttttattgg agcggccgtc ctcccggaag tgcggaacag
      301 gtcggccccg gttccatgca cctgaagacc tctgcgcctg gccccggcct aaaaaaggga
      361 ggccggcttt ttgcgtcctc cttccccctt tttnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn ngtcctcctt cccccttttt cattgcggtt ccgcaccgga
      481 ttgaccatga tacgggctgc tccaacccgc catgtacacc gcacgcctcc aatacgacag
      541 gattatcatt acgggcgcat catccggctt cggggaagcg tttgccggaa cgctggcccc
      601 ccatacggcg gaactggtgc tcatcgcccg gaatgaagca gccctgcgcc agcttgccgc
      661 cgccctggaa aaacgccatc ccggccttca cgcctccgtc cttccctgcg acctggcgga
      721 cgaaacctcc ctgaatacgc tcatttcacg cctggacagc cttcctccgg gaagaaccct
      781 tctgattaac aacgccggag ccggtgatta cggagatttt gcggacggcc gctgggaaaa
      841 aatacgttcc ttgctgcgtc tgaacgtgga aagcctcacc cgcctgtgcc atgcgctgat
      901 ccccgtcatg aagaggaacg gaggggatat catcaacctc agttccctgg gagccctcct
      961 gcccattccg gattttgccg tttatgcggc caccaaggct tatgtttcca gcctgtcgga
     1021 agcgctgcgg ctggaactgc gcgaacacgg catccgcgtg atggccgtct gccccggccc
     1081 ggtttccaca ggcttcggga aagcggcgcg ccgccccggc ttcaccggaa acatgatgcc
     1141 ggggcgcaat gcttttgaca cgtccgttga aaccgtcgtg aaagacagcc tccgcgccct
     1201 ggcccgcgga cgagcccggg tctttcccgg catgaaaata cggctggccg ccatgctgct
     1261 ggctgccgcc ccgctggggc tgatacgcct tttcatgggc agacgtcccc ggaaagtcct
     1321 gccggcatcc aatgattctc cttcttcagc atcatgaaag cgcaccgccg cggaataccc
     1381 cgcctcacct ggttcaagct cgcagtctgc ctctgcctgg cagccatggc tgtctccctg
     1441 ttcacgccgt accccgccca gatcatacgc gccctgaaag gggagccgga acaggcgccg
     1501 ccggattccg gaacctgtcc gccccaggaa tccccccccg cggaacagcc cgggcaggaa
     1561 gaacagccaa taaccgctgc gccatctgcc tccggaaagg aacccgggga tgaacggctg
     1621 tacactccat ggacgccgga gcgcacaatc gcccttcccc cggtgcagta ccccccattt
     1681 cctcccgtgc ttcccacaag gcccgcggaa ggggccatga cccatattta tgaactggcc
     1741 cgcggcctga acctgaaaac gcaggttaat ttccaacccg gcaccctggc ctccagggac
     1801 agggaaacca aaagcaatta ccagatgacc ctttccctga acgtcaaaca gcccaaagca
     1861 ttgacgaaaa aggaagacat tctgaagcgc aatccgaaac tggaacccat gcttcccggc
     1921 ctgtccaccc tgttcaggca tgcgcgcgtc tccccttatt acgggcaggt ttacgtccgc
     1981 aaacagacgg aaatccgtaa aaacctggcc tccctgctca aacttctgga ccgccataat
     2041 tattacgata cggagaccat tctggaaacc gcctatccgg acacgggaag gaagcttctc
     2101 tggctgcaaa gtgaaatgga tgttgtttcc gacggctcgg acggggaccg gctggccgcc
     2161 atgccggata aaatcctgaa aagctccttc tatcagccca gcacttccta ccgctggaaa
     2221 aagcgcacag acaagcccaa tcccctgctc aacccctggc agcagaggct ggcctcctac
     2281 aagaagacgc tggaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta
     2341 aaaagccccc gccgcggaaa aaacggctct gcgccgcaaa atagaccatg cggaacgggt
     2401 cattgaggaa ctgaagcggt acagcttcct gatctcggaa tacgatccgt tcatcgtggt
     2461 tccgctggga gtcgtcaacc agtcctctcc cttctccccg cagttcgggg actatgccgt
     2521 cgtcatcgtg ggagacaagt tgtacccggc cctggtggga gacgccgggc cgcgctacaa
     2581 aactggggag gggtccctgc gcctgagccg tgaaatcaac cccaaggcgg gcccttacag
     2641 ccgccccgtc tccgacctga aggtaagtta cctgattttt cccggttccg cagagcctga
     2701 ggccggccca cccgactatg aaaaacttac ggacagatgc cgggaacttc tcaacgaaat
     2761 aggcggcatg gggaaaggat tcaaactcca ccaatgggaa gacctgctgg cgcccaagcc
     2821 gccgcccgcc ccaaaaccgg aaagcggcgg cacagcggaa acatccgcgg ataagcggaa
     2881 aaaggaggaa gcccccggga acaagcccgc gccggccccg gcttccccgc aaactgcacc
     2941 ggcttcatca gccgcaaaaa gcgccgcata agctacagag cctccttcca ggaaaaaaga
     3001 aaggctttcc acatccggaa ataaacgtct ccgcgcgcaa tcttcacctt cccggaagac
     3061 ggatgccgcc gatccgcccg ctggagcaat tccagcgtgt ccagccccag ccaggcgggc
     3121 aggacagtgg aaaaacgcag ccgcccgttc cgcagagatt ccgcgtaaaa caccccttcc
     3181 ttcagccagc gttccgcacg gtccagccat ggacggtcat ctggggcggg caagtagctt
     3241 cttccgcggg cgaggtcttc ctcccggtca cgcagaatat tcaccaactg cagccccatg
     3301 ccgtagtgcc tgccccagcg caaaagctgc tcctccggct gcgtgctgaa gcccggcccc
     3361 tgagccagca aacccagcct ggtccagaat tcccctacgc agccggccac catgtaagta
     3421 taatgttcca gctgttccgg acatgagacg gcagcatggc tttcaaaatc catcaactcc
     3481 cacgcctgtc cctccacgat ggaggccagc acctgccgca ccagcatccg ttcatgcggg
     3541 gaaaggtttt ccagcagcgc caggcattcg gggaaacggg ccagcaattc cgcctccccc
     3601 cggtgcgtct ggattgtgga aagaacgccc agacggccaa aacaggtggc gacgacctcc
     3661 cgggaggcgg cgccggccac cgtctccccc atcatcctca aaagcgccag gcgctcccgg
     3721 caatccatat ccacggcatc cgccaccgta tccgtggcac gcgccagcag atagcccacg
     3781 gccacgccgg agcgcatttc ccggggcagg aagcgcatgc tcagataaaa ggaccgggag
     3841 acggccgata gaagatggtc aaggcctttc atgatttctg caggcccagc ctgtgcatct
     3901 tataatggag tactcgcggg gaaatgccca gttcccgggc cgcggcggac atattgcccg
     3961 gagaacgttt caatgcggct acaatcaatt cccgttcaaa ggaatctacc atggtcggca
     4021 aatcggcagg agcctccccg tcacgggaat aagggacgtc atggctgggc atctgaaggg
     4081 aggcgggaag gttgtaaccg taaatgcagt cgtcctgcgc cgtaatgacg gcacgttcca
     4141 tgcagttttc cagctcccgg acgttgccgg gccagtggta agccatgagc atgttgatgg
     4201 cgggagtgga cagccgtttg atatcctttc cgtatttcag gttgaacttg gaaagaaaat
     4261 gttccgccag cagcatgacg tcccccttgc gtttggagag gtccggcata acgatgggga
     4321 aaatgttcaa ccggtaatac aagtcttccc ggaacttgtt cgccgccatc aattcctcca
     4381 gattgcggct ggtggcggcg atgaagcgca cgtctgcatg aagctccctg ttgcttccca
     4441 cgcgggaaaa ggttttttcc tgaagaaagc gcagcagctt cacctgcatc tgaaggctta
     4501 aatccccgat ttcatccaga aacagggtgc cgccgtccgc cgcctccgcc cggccgatgc
     4561 ggcggctggt ggccccggta aaagaaccct tttcatgacc gaacagctcg ctttccagca
     4621 ggttctccgg catggccgca caattcagcg tgaccagcgg cttatccttt ctgccgctca
     4681 gattcacc
//
