GCA_004210255.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaNEJFNINO_1 (original name was: gnl|Prokka|NEJFNINO_1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	proteusin	327,650	351,095
Region 2	NRPS,cyclic-lactone-autoinducer	689,780	742,945	ulbactin F/ulbactin G	NRP	28%
Region 3	lanthipeptide-class-ii	1,615,085	1,638,036
Region 4	ranthipeptide	3,806,206	3,827,914
Region 5	NRPS	4,739,247	4,786,647

gnlProkkaNEJFNINO_1 - Region 1 - proteusin

Download region GenBank file

Download region SVG

Location: 327,650 - 351,095 nt. (total: 23,446 nt) Show pHMM detection rules used

proteusin: (PoyD or TIGR03793)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaNEJFNINO_1 - Region 2 - NRPS,cyclic-lactone-autoinducer

Download region GenBank file

Download region SVG

Location: 689,780 - 742,945 nt. (total: 53,166 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 2, location 689779 - 742945:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Cys - Cys) + (Cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_653

Search NORINE for peptide: strict or relaxed

AMP-binding (527..920): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNLSLIWK
Nearest Stachelhaus code(s):: Cys DLFNLSLIWK (74% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg1_654

Search NORINE for peptide: strict or relaxed

AMP-binding (548..944): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNMSMIWK
Nearest Stachelhaus code(s):: Cys DLYNMSMIWK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1974..2372): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg1_655

Search NORINE for peptide: strict or relaxed

AMP-binding (28..431): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: PLPAQGVLCK
Nearest Stachelhaus code(s):: diOH-Bz PLPAQGVLCK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaNEJFNINO_1 - Region 3 - lanthipeptide-class-ii

Download region GenBank file

Download region SVG

Location: 1,615,085 - 1,638,036 nt. (total: 22,952 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg1_1452

ctg1_1453

Leader:

MMNQKKRVERQDVMNVAGDLGKEMEEFKKADLVGA

Core:

ESELLVTPKFTPLFTILCC

Core with dehydrations:

EDhaELLVDhbPKFDhbPLFDhbILCysCys

antiSMASH-DB 4.0 matches for ctg1_1453

100.0% NZ_CP035945.1: Lanthipeptides: PMF13cell1_RS28255_lanthipeptide

1

ESELLVTPKFTPLFTILCC

19

ESELLVTPKFTPLFTILCC

1

ESELLVTPKFTPLFTILCC

19

36.8% NC_013510.1and 2 others:

NC_013510.1 Lanthipeptides: TCUR_RS26055_lanthipeptideNC_013510.1 Lanthipeptides: TCUR_RS26055_lanthipeptide

Lanthipeptides: TCUR_RS26055_lanthipeptide

1

ESELLVTPKFTPLFTILC

18

+ LL+ KF+ T+LC

1

SASLLLCDKFSAFSTLLC

18

36.8% NZ_JAEVFI010000019.1: Lanthipeptides: JJV70_RS15420_lanthipeptide

3

ELLVTPKFTPLFTILC

18

LL+ K + TILC

3

SLLICDKHSGASTILC

18

with 11 more sequence matches

33.3% NZ_FMHI01000010.1: Lanthipeptides: H180DRAFT_RS29175_lanthipeptide

1

ESELLVTPKFTPLFTILC

18

+ LL+ KF+ T+LC

3

SASLLLCDKFSSHSTLLC

20

31.6% NZ_CP036524.1: Lanthipeptides: LAJLEIBI_RS18025_lanthipeptide

11

TPLFTILCC

19

T FTI+CC

7

TTFFTIICC

15

20.8% NZ_CP048626.1: Lanthipeptides: GXM18_RS04600_lanthipeptide

3

ELLVTPKFTPLFTILCC

19

++ ++ FTI CC

8

DIYISIDNASFFTIFCC

24

20.6% NZ_KN125582.1: Lanthipeptides: DJ91_RS27545_lanthipeptide

6

VTPKFTPLFTILC

18

VTP+ TP T+L

3

VTPRTTPATTLLA

15

20.5% NZ_BMJA01000007.1: Lanthipeptides: allorf_022673_022864_lanthipeptide

1

ESELLVTPKFTPLFTILC

18

ES L + PK T F ++C

8

ESSLNMRPKDTIFFYVVC

25

20.0% NZ_CP011152.1and 3 others:

NZ_CP011152.1 Lanthipeptides: WR47_RS28830_lanthipeptideNZ_CP011152.1 Lanthipeptides: WR47_RS28835_lanthipeptideNZ_CP011152.1 Lanthipeptides: WR47_RS28830_lanthipeptide

Lanthipeptides: WR47_RS28835_lanthipeptide

6

VTPKFTPLFTIL

17

VTP+ TP TIL

3

VTPQATPTVTIL

14

17.9% NZ_CP045695.1: Lanthipeptides: allorf_0915160_0915312_lanthipeptide

7

TPKFTPLFTILCC

19

T + T FTI CC

27

TIQCTQFFTIYCC

39

16.7% NZ_MCGH01000003.1: Lanthipeptides: BEI61_RS31750_lanthipeptide

11

TPLFTILCC

19

T LF+I CC

28

TTLFSIFCC

36

15.4% NZ_QGDL01000005.1: Lanthipeptides: A8806_RS26140_lanthipeptide

11

TPLFTILCC

19

+ LFTI CC

31

SNLFTIYCC

39

15.2% NZ_CP040409.1and 4 others:

NZ_CP040411.1 Lanthipeptides: FE334_RS06825_lanthipeptideNZ_CP040422.1 Lanthipeptides: FE323_RS09430_lanthipeptideNZ_JH601103.1 Lanthipeptides: HMPREF9703_RS02710_lanthipeptideNZ_NAQX01000046.1 Lanthipeptides: B8A46_RS06295_lanthipeptide

Lanthipeptides: FE336_RS08590_lanthipeptide

12

PLFTILCC

19

P+FT+ CC

26

PVFTVACC

33

14.7% NZ_MCGI01000004.1and 1 other:

NZ_MPDJ01000004.1 Lanthipeptides: BLA28_RS33805_lanthipeptide

Lanthipeptides: BEH84_RS33640_lanthipeptide

11

TPLFTILCC

19

TP +I CC

26

TPFLSIFCC

34

ctg1_1460

Leader:

MELTDIFLDSLCGN

Core:

YCPKKIQHSLNLLELEIKRKKNFINQNCCQQIQNCRPLGIC

Core with dehydrations:

YCysPKKIQHDhaLNLLELEIKRKKNFINQNCysCysQQIQNCysRPLGICys

antiSMASH-DB 4.0 matches for ctg1_1460

100.0% NZ_CP035945.1: Lanthipeptides: allorf_1632970_1633179_lanthipeptide

1

YCPKKIQHSLNLLELEIKRKKNFINQNCCQQIQNCRPLGIC

41

YCPKKIQHSLNLLELEIKRKKNFINQNCCQQIQNCRPLGIC

1

YCPKKIQHSLNLLELEIKRKKNFINQNCCQQIQNCRPLGIC

41

allorf_1624403_1624558

Leader:

MRIHILQVINIG

Core:

HKGCRNQIVLKEMFSFRNLENYKQNFWSFLSGFDEIIIA

Core with dehydrations:

HKGCysRNQIVLKEMFDhaFRNLENYKQNFWDhaFLDhaGFDEIIIA

MIBiG 3.1 matches for allorf_1624403_1624558

9.8% BGC0002120 (NQE92413.1): Lanthipeptide: nocathioamide A

23

KQNFWSFLSGFDE

35

K+N SG+D+

5

KRNEEVAESGYDD

17

allorf_1633279_1633470

Leader:

MLIDLCNALSVSPDYLMAQELRLPSDNSGDIYFA

Core:

VFEKVKKLPPDQVEMLDCIMDTVIKKNGI

Core with dehydrations:

VFEKVKKLPPDQVEMLDCysIMDDhbVIKKNGI

antiSMASH-DB 4.0 matches for allorf_1633279_1633470

100.0% NZ_CP035945.1: Lanthipeptides: allorf_1633279_1633470_lanthipeptide

1

VFEKVKKLPPDQVEMLDCIMDTVIKKNGI

29

VFEKVKKLPPDQVEMLDCIMDTVIKKNGI

1

VFEKVKKLPPDQVEMLDCIMDTVIKKNGI

29

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg1_1453 - Class II

Cleavage pHMM score: -3.00

RODEO score: 0

Monoisotopic mass: 2081.1 Da

Molecular weight: 2082.5 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2100.5 Da N = 1; 2118.5 Da N = 2

ctg1_1460 - Class II

Cleavage pHMM score: -6.60

RODEO score: 0

Monoisotopic mass: 4852.5 Da

Molecular weight: 4855.8 Da

Number of bridges: 1

allorf_1624403_1624558 - Class II

Cleavage pHMM score: -5.70

RODEO score: 0

Monoisotopic mass: 4667.4 Da

Molecular weight: 4670.3 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4688.3 Da N = 1; 4706.4 Da N = 2

allorf_1633279_1633470 - Class II

Cleavage pHMM score: -3.60

RODEO score: 0

Monoisotopic mass: 3311.8 Da

Molecular weight: 3314.0 Da

Number of bridges: 1

gnlProkkaNEJFNINO_1 - Region 4 - ranthipeptide

Download region GenBank file

Download region SVG

Location: 3,806,206 - 3,827,914 nt. (total: 21,709 nt) Show pHMM detection rules used

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaNEJFNINO_1 - Region 5 - NRPS

Download region GenBank file

Download region SVG

Location: 4,739,247 - 4,786,647 nt. (total: 47,401 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 6, location 4739246 - 4786647:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_4154

AMP-binding (22..445): X

nrpys: Orn, Lys, Arg

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: -MECIGGPLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

ctg1_4157

AMP-binding (474..858): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DALAVGQVAK
Nearest Stachelhaus code(s):: Trp DALAVGQVVK (50% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (1491..1879): X

nrpys: Gly, Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVIQIMIVYK
Nearest Stachelhaus code(s):: Ala DILQITLVYK (53% 8Å match)
Stachelhaus code match:: 60% (weak)