LOCUS       c00010_gnlPro..         7857 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PCLAGGJO_10.
ACCESSION   c00010_gnlPro..
VERSION     c00010_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 23:46:08
            Original ID  :: gnl|Prokka|PCLAGGJO_10
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7857
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7857
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[4619:7590]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      4620..7590
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7857
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7857
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..131)
                     /locus_tag="ctg10_1"
                     /transl_table=11
                     /translation="MLFNYTTHFLVLYYFSSSSPTKVRGVREELKLFLFYFFISTFY"
     CDS             148..480
                     /locus_tag="ctg10_2"
                     /transl_table=11
                     /translation="MKREIIAYKGYFKEFFENLDAGTQDKILYVLMLLQTQDRIPLKFM
                     RLIEEGLYELRIEYQSNIYRIFFCFDEGRIVILFNGFQKKTEKTPKKEIDKAKILRKEY
                     YGSKNK"
     CDS             461..766
                     /locus_tag="ctg10_3"
                     /transl_table=11
                     /translation="MEAKTNEEFFNVSALIDERFGKEGTASRAEAEEKAYAFYTGQIIE
                     DARKKAKITQAELARRIGSDRSYISRVESGQTEPKVSTFYRIMNALGCKIEFSMIL"
     CDS             complement(836..2950)
                     /locus_tag="ctg10_4"
                     /transl_table=11
                     /translation="MKHFTIFQGFYYAIAEMTEEEIVSTIGSFTYREKVEEIRRIFAEQ
                     GEKAANEKKKELPAIAFSASYRGRRTKVNLVKYLGHIVIDIDHLSKEELARILPIIKRC
                     DYTRIAFISPKGMGVKIIVRACHPDETLPETLQEIEDFHHAAYTRLVSFYTELCRIEID
                     TSGQDVARTCLFSYDPEIYFNPNADAFLVDQPQASYKISNRKNLSGSKQQTPPDGTPTN
                     EDTALNAHSANASLVLTLTYYHNKSEKYIAGNRNNYLHHLSCTFNRYGIPQEETSAFIK
                     SQFTDFPADETVSLINSAYAHTDEFNTCKLNGTQKRILRIEQYISEHYETRYNEVLHIM
                     EYRRRRPDTEKPEPFRILDEMMENSIWIEINELGYPCTVKTIENLIYSDFSQSYHPIRE
                     YFELLPEWDGTDYIRILADSVQTSHQEFWAECLERYLVAMCAAATQENIVNHTVLLLCS
                     EIQNIGKTTFINNLLPPELRAYLSTGLINSNNKDDLAKITQAMLINLDEFEGMSGRELN
                     AFKDLVTRKVISFRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHPYSIQF
                     ITINYAQLYAQIKYLLNKPGYQYWFTQADNERLEENNEAFIFHSPEEEMVLTHIRKPER
                     FEKVYYLTVSEIAELIRERTGYQYSIGSKIQLGKVMTKHHFESKKGNNGRRYAVFIIDI
                     EQVKSNRLYE"
     CDS             complement(3037..3267)
                     /locus_tag="ctg10_5"
                     /transl_table=11
                     /translation="MNYKEDSFLIRTYTKAELAHLYNPHVCLKVALQILRRWIIYNLPL
                     LHELEQEGYRARNRLLSPKQVATIIHFLGEP"
     CDS             3461..3964
                     /locus_tag="ctg10_6"
                     /transl_table=11
                     /translation="MAMTVSYSVVPRKNPAKKSEPAKYYAQAQASGELDFEELCEAITS
                     RSTCTETDVRAAISGILYEVKRALKAGRIARLGDLGSLQIGLNSEGAASVKEFSGSMIK
                     GAHLIFRPGKTLAELMKILSYQQVLTRAVAQAGAGDGGGEDPDKNPDSGGDGSGDEEAP
                     DPTV"
     CDS             4024..4191
                     /locus_tag="ctg10_7"
                     /transl_table=11
                     /translation="MKEIVNRILDVIMYLIPFFGKRKRDKVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             4178..4627
                     /locus_tag="ctg10_8"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDEDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(4620..5348)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 73.7;
                     E-value: 6.1e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg10_9"
                     /sec_met_domain="Radical_SAM (E-value: 7.2e-31, bitscore:
                     97.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFKLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQGKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(5362..7590)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg10_10"
                     /sec_met_domain="Gly_radical (E-value: 6.2e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1174.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     IWNRCLEALAEERENNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDML
                     KFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLN
                     RDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 atatagaaag tagaaataaa gaaataaaat agaaaaagtt tcaattcctc tctcactcct
       61 ctcacttttg tgggtgaaga tgatgaaaaa taatataaaa cgaggaaatg tgttgtatag
      121 ttgaacaaca tttcttatat ttgcgttatg aaaagagaaa taatagcata taaaggatac
      181 ttcaaagaat tctttgaaaa tttagatgct ggcacccaag ataagatatt gtatgtgcta
      241 atgttattgc agacacaaga cagaatacct ttgaaattta tgcggttgat agaagaagga
      301 ctttatgaac ttcgtatcga gtaccaaagc aatatatata gaatattctt ttgtttcgac
      361 gaagggcgta ttgtgattct gtttaacggt tttcaaaaga aaaccgaaaa aacaccaaag
      421 aaagaaattg ataaagcaaa gatattaaga aaggagtatt atggaagcaa aaacaaatga
      481 ggaattcttt aatgtcagtg cattaataga tgagcgtttt ggtaaggaag gaacagcttc
      541 tcgtgcagag gcagaagaaa aggcctacgc cttttataca ggacagatta tcgaggacgc
      601 aagaaaaaag gctaaaatca ctcaggctga attagcccgg cgtatcggct ctgaccgttc
      661 ttatatttca agagtagaaa gtggtcagac agagcctaaa gtatctactt tctatcgtat
      721 aatgaatgcg ttaggctgta aaatcgaatt ttcaatgatt ttgtaaaaac aataaatgca
      781 gagtatctgt caagatgact ctgcattttg ctatattcac aaggctttcc cgcttctatt
      841 catacagcct gtttgatttt acctgctcga tatctataat aaatacggca tatcggcgcc
      901 cgttatttcc ttttttagat tcaaaatggt gcttagtcat caccttcccc aactgtattt
      961 ttgatccgat ggaatactga taaccggtac gctcacgtat tagttccgca atctcactga
     1021 cagtcagata ataaaccttc tcaaaacgtt ccggcttgcg gatatgcgta agaaccattt
     1081 cttcttccgg tgaatgaaag atgaaagcct cgttattctc ttcaagccgt tcattgtccg
     1141 cttgcgtgaa ccagtattgg taccccggct tattcagcag atatttgatc tgagcataga
     1201 gctgcgcata attgatggta ataaattgga tggaataagg atgaaaacaa aggaaacgcc
     1261 ggttgcctgt cgtgtcgtga agcacttcct gataattgca ggttcctgca aaagaggccg
     1321 tatgagggaa gttttgcgaa cggcgtgcat atggcagacg gaaactgatc actttgcgcg
     1381 ttaccaaatc tttgaatgca ttcagttccc gaccgctcat cccttcaaac tcgtcgagat
     1441 tgatcagcat ggcctgcgtt attttggcta aatcatcttt attgttcgaa ttgattagtc
     1501 cggtagagag ataagcacgc agttcgggag gcaggagatt attgataaag gtagtctttc
     1561 ctatgttctg tatctcgctg catagcagaa gtaccgtgtg attgacaata ttctcctgag
     1621 tagccgctgc gcacatcgcc accagataac gttccaggca ttccgcccaa aactcctgat
     1681 gactcgtctg gacactatcc gccaggatac ggatatagtc ggttccgtcc cattcgggca
     1741 gtaattcaaa atactcgcga atgggatgat acgactggct gaaatcggag taaatcagat
     1801 tttcgattgt tttgacagtg cagggatatc ccagctcgtt gatttctatc cagatactgt
     1861 tttccatcat ctcatccaag atgcggaaag gctccggttt ctcggtgtcg ggacgccttc
     1921 tgcgatattc catgatatgc agcacttcat tgtagcgtgt ttcgtagtgc tcgctgatat
     1981 attgttctat ccgtaatatt cgtttctgcg tgccattcag tttacaggta ttaaattcgt
     2041 cggtatgagc gtaggcgcta ttgatcaaag agactgtctc gtccgccgga aagtctgtaa
     2101 actggctttt gataaaggcg gatgtttcct cctgcggaat gccgtagcga ttgaaggtac
     2161 atgagagatg gtgcagataa ttgttccggt tgcctgctat gtatttttcg gacttgttgt
     2221 gatagtaagt taaagtcaac accaaagaag cgttggcgga atgggcgttg agtgctgtgt
     2281 cttcattcgt cggggtgccg tccggaggag tctgttgttt actgccggag agatttttac
     2341 gatttgaaat cttgtaggat gcctgaggtt gatctacaag aaaagcgtca gcgttcggat
     2401 tgaaatagat ctccggatcg taagagaaca gacaagtgcg tgccacatcc tgaccggagg
     2461 tgtcgatttc tatccgacag agttcggtgt aaaaagaaac gagtctggtg taagctgcat
     2521 gatggaagtc ttctatttcc tgaagggttt cgggcaatgt ctcatccgga tggcatgcgc
     2581 gcactatgat tttcacgccc atccctttgg gactgataaa ggcgatgcgg gtgtaatcgc
     2641 atctcttgat gatagggagg atgcgtgcca attcttcttt gctgaggtgg tcgatgtcta
     2701 tcacgatgtg tcccagatat ttgacgaggt tcactttggt acgccttcct cggtagctgg
     2761 cggagaaggc gattgccggc aattcttttt tcttctcatt ggcagccttt tctccttgct
     2821 ctgcaaagat gcggcgtatt tcttcgactt tttcccggta ggtaaaactg ccgatagtgg
     2881 acactatttc ttcttcggtc atctctgcta ttgcgtagta gaaaccttga aatatggtga
     2941 aatgtttcat ttttattttg attattgttt gtcgatgcaa aggaacttct ttgcggaaat
     3001 gtgtaaagga actttgtata aaatggcaat aagttactac ggttcgccca aaaagtgtat
     3061 gatagttgcc acttgtttcg gcgaaaggag acggttgcgt gcgcggtagc cttcctgttc
     3121 gagttcgtgc aggagaggga ggttgtaaat aatccaacga cgaagtattt gtaaagcaac
     3181 tttgaggcag acatggggat tgtaaaggtg agccagttcc gctttggtat aggtgcggat
     3241 aaggaaagaa tcttctttgt aattcatgat gttttcttct tgttttatgc tataaagtta
     3301 cataaaattc cttgtttttc aaaggtttga cttcattttt attcgttggg aatcaagaaa
     3361 cttcgttggg gaatatggct tgtccggcta tccttatctt tgtgctgtta acaagaaagg
     3421 gatgcatcct acgtattata aactttaaaa ttattttatt atggcaatga cagtatctta
     3481 ttcagtggtg ccgcgcaaga atcctgcaaa aaagagtgaa ccggcaaagt attatgcaca
     3541 ggcgcaggcg tcgggtgagc tggactttga agagttgtgc gaagcgatca ccagtcgttc
     3601 tacttgtacg gaaacggatg tacgtgcggc tatttcgggt attctttacg aagtgaagcg
     3661 tgcgttgaag gcaggaagaa ttgcgagact gggtgatttg ggcagtttgc agataggact
     3721 gaacagtgaa ggtgcggcat cggtcaagga attttccggt tcgatgatta agggggcgca
     3781 tcttattttc cgccccggta aaacgctggc ggaattgatg aagattctga gttaccagca
     3841 agtgctgact cgtgcggtgg cgcaggcggg tgcaggcgat ggcgggggag aagaccctga
     3901 caagaatccg gattccggtg gtgatggctc cggtgatgaa gaggctccgg accctacggt
     3961 ataattggca gacgggtcaa ggtaataacg aattagaata acttccatta atattttgat
     4021 aagatgaaag aaatagtaaa ccggatttta gatgtgatca tgtatctgat tcctttcttc
     4081 ggaaagagaa agcgggacaa ggtggtgaga gaagtgcgct atcatactac ttgtaaggag
     4141 gtgtgcaaag tgaaaacgac ggaaagggaa aaggaacatg agaaagatta gtctgattgt
     4201 gatccattgt tccgccaccc gtgtcgattg cgactttacg gcaaaggatg tggatacggc
     4261 tcaccgttat cgtggctttt cgtgctgggg gtatcattat tatatacgca agtcgggcga
     4321 gattgagccg atgcgggatg aagatacggt cggagctcat gcacgtggct acaatgcgat
     4381 cagcttaggt gtatgctatg agggcggtct ggatgagaat ggaaaagcgg cggatacgcg
     4441 tacaccccgt cagaaggagg cgctacatcg cttggtacac gaattgttgc agcgttatcc
     4501 ggaagctaaa gtagtagggc atcgggattt aagtcccgac accaactata atgggatcgt
     4561 agatccttgg gaacggatta aagaatgccc ttgctttgag gtcattggag aattctccat
     4621 taattaacta ctaccgtagt gaaatactct ttgaatacct ccgcagcttt ttcaagctgt
     4681 tcgggggtat tttcttttac ccctttcatc ttgtattcct gtcccatcgc ttcgtactta
     4741 tgtacgccca gtgtatggta aggcagtatt tccacccgtt ggatcatctt gtattttccg
     4801 agtgcttctc ccagtgcgcg tatgtcttct tcaaaatcgc tgtatccggg cactagcaca
     4861 taacgcagcc agaaaggttt tccctgttct tccagccagg cggccgtgcg gatggtttgc
     4921 tcgttgcttc ttccggtgag cgtctgatgg cggttgggat tgaattcctt tatatccaat
     4981 aacacaagat cggtcagctt gaataattct tctacgtctt cgttccaaag tcctccgttg
     5041 ctgtccagac agacatggat gcctttttct ttcagttcgc ggaccagcgg gacgagtgct
     5101 ttcgcctgaa acgtcggttc gcctccagag aaagtgattc ctcctcgttt cccgaagaag
     5161 gggcgctggc tcattgccat gcggacgatt tcttccggtg gggtaggcgt gcctcccttt
     5221 cctgctatcg tatccggatt ggcgcaatag aggcagcgga agttgcatcc ttgaagaaaa
     5281 acgacgagcc gtaagcccgg cccgtcgaat gttcccatac tttcgtatga atgtacgttt
     5341 atcatcatat agagtaatag attacatacg ttcgtggaag ctacggctga taacttccag
     5401 ttggtgttcg cggctcagct tcacgaagtt tacggcataa ccggaaacgc ggatggtcag
     5461 ctgcggatag ttctccggat gctccatggc atcgtatagc atatcacggt tcagtacgtt
     5521 tacgttcagg tggtgggcac ctttggtgaa gtaaccgtcc atcatcgtta cgaggttctc
     5581 gatacggtct tcgtccgtag cacccagtga tttcggaacg atagagaagg tgttgctgat
     5641 accgtcctgt gagtcacggt aacgaagttt cgctacagag ctcagagaag cgatcgcacc
     5701 attcttatcg cgtccgtgca tcgggttggc acccggagcg aaggcaacac ctttggcacg
     5761 tccgtcggga gtagcaccgg tctttttgcc gtacatcaca ttggaagtga tagtcagcaa
     5821 ggagagggta ggacgggcat tcttgtaaac aggcaatttc ttcaattctt cgctgaagaa
     5881 gtataccagg tctacgccga ggtggtctac cttgtcgttg tcgttaccga agcaagggaa
     5941 ttctccttcg atgtcgaaac cttctgtcag gccgatatcg ttgcggcggg cagttacttt
     6001 cgcatatttg atggcagaca gtgagtcgag tgcgatggaa agtccggcaa caccgtaagc
     6061 aaggtttatg cgcgggttcg tatctacaag agccatctgg gccttctcgt agtaatactt
     6121 gtcgtgcata tagtggatga tgttcatcgc ttcgttgtat acgcgggcga tttcggtcaa
     6181 cactttcttg tagttgtcca ttacttcttc gaacttcagc atgtcgctgg tcagtacggg
     6241 gatgttcttc accatgactg taccggtatt ctcgcaacgt ccgccgttga tggcaagcaa
     6301 cagggctttg gccaggttgc aacgggcacc gaagaactga atctgcttgc ctatttcctg
     6361 gtatgatacg cagcaggcga ttccgtagtc atcggactga cgtacttcac gcatcaggtc
     6421 gtcgttttcg tactggatag aagaagtgtc gatagaaacc tttgcacaga attctttgaa
     6481 tccttccgga agttccgggc tccagagtac ggtcaggttc ggttccggtg aagggccgag
     6541 gttgtacaat gtctgcagaa aacggaagga ggtctttgtc actttggtac gtccgtcgtt
     6601 gagacgtccg cccagagatt cggttaccca ggtcgggtcg cctgcaaaga tgtcgttgta
     6661 tgattgcata cgcaggtggc ggaccatacg cagtttgatg acaaactggt cgatcagctc
     6721 ttgcgcgaac gattcggtga tggttccttt gctcagttca tattccagat agatatcgag
     6781 gaaagaagaa acgttaccca gtgacatagc ggcaccgtct tgttctttga cggcggcaag
     6841 gtaagccata tatacccact gtacggcttc ttgtgccgtg taagcgggac ggctcaggtc
     6901 gagaccgtag tattcgccca tcactttcat gtctttcagt gccttgatct gttctgccac
     6961 ttcttcgcgc aggcggatgc gggcctctgt catcggaccg gtgaggttgt gcaaatcttc
     7021 cttctttgct tcgatcagac ggtcgatgcc gtaaagagcc atacggcggt agtcaccgat
     7081 gatgcgtccg cgtgcataat tgtcgggaag tccggtaagg aatcccagcg aacggaagga
     7141 acggatttct tccgtatata cgtcgaatac tccgtcgttg tgcgtcttgc ggtagtgagt
     7201 gaaaatatct tttacgcggt cgtccacttc cacgccgttt tcgtgacagg ctttgctgac
     7261 tacgttgata cctccgaaag gcttgatggc acgtttcaga agttcgtctg tctgcaggcc
     7321 gacgatcagt tcgttttcct tgtcgatata tccggccttg tgggaagtga tggttgatac
     7381 ggtaacatta tctaatgagc ggacaccgtt gttttctctt tcttctgcca gtgcttcgag
     7441 gcagcggttc catatggctt tggtacgttc tgtaggtcct tcgaggaatg aagcatctcc
     7501 gtaatacgga gtgatgttat gacttacgaa atctctgacg ttgatttcgc tgctccaaag
     7561 accgtcttta aagatcttgt ttaattccat aaaatgataa aattagaggt tgtagttatc
     7621 ctattcttta gagcgcacaa agttacaatc atttttcata taatcagcat aaatatgctc
     7681 tattttatgc ttttcaacat aaaataccta ttaattgcta tccttaggaa aagattagat
     7741 taaattttta gtgaaaactc ttgcaagttt aaaatatctt cgtacctttg caccgctttt
     7801 aacgaaaagc acttctgaca aggaagtttt ggagaggtgg cagagtggtc gattgcg
//
