LOCUS       gnlProkkaBNFNICLF_82   11819 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BNFNICLF_82.
ACCESSION   gnlProkkaBNFNICLF_82
VERSION     gnlProkkaBNFNICLF_82
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-30 21:37:09
            Original ID  :: gnl|Prokka|BNFNICLF_82
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11819
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..11819
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[2505:6819]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2506..6819
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..11819
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..11819
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             3..1283
                     /gene_functions="transport (smcogs)
                     SMCOG1186:polysaccharide export protein (Score: 72.7;
                     E-value: 3.7e-22)"
                     /gene_kind="transport"
                     /locus_tag="ctg82_1"
                     /transl_table=11
                     /translation="MVVTAEAILNRFTNKLEIRGAVYRPGIYQLSGKLNTIRELVHEAQ
                     GLTGDAFLNRAVLYRQREDLTSEVVQIDIKSIMDGTSPNLALMKNDILYIPSIHDLEDR
                     GNVTVHGEVAQPDSYPYADNMTLEDLIIQAGGLKEAASTVRIDISRRIKNPRSTADNDT
                     IGQMYTFSLKDGFVIDGQPGFILEPYDQVYVRRSPGYQAQQNVAIEGEILFGGNYAMTS
                     REERLSDLVNKAGGPTNYAYLRGAKLTRVANASEKKRMGDVIRLMSRQLGEAMIDSLGI
                     RVEDTFTVGIDLEKALSNPKSNADLVLREGDVISIPKNTNTVTINGAVMVPNTVSYMKG
                     KNVDYYLNQAGGCSDNARKSKKFIVYMNGQVTKVKGSGKKQIEPGCEIIVPGKAKKKGN
                     IANILGYATSFSSLGMMIASIANLIKK"
     CDS             1294..2442
                     /locus_tag="ctg82_2"
                     /transl_table=11
                     /translation="MIEKQSYQEPFSEVDKRKDDEMEIDLMAIFYKIITVRGVLCKAAG
                     VGVIIAIVVALSIPKQYTVKVTLSPEMGNAKGSNGLAGLAASFLGDGALIGESTDALNA
                     SLSADIVSSTPFLLELLEMEVSVSKKDDKMTLGSYLDEESSPWWDYLIGFPGIVIGGVK
                     SLFGEDTVPASGGRQGTIELTKEVNEKINFLKKKISASIDKKTAITSITVTLQNPQITA
                     VIADSVVHKLQEYIIGYRTSKVKEDCAYLERLFKERQQEYYAAQRKYADYVDAHDNVVL
                     QSVRAEQERLQNDMSLAYQIYSQVANQLQVSRAKVQEEKPVFAVVEPAVVPLKPSGMGL
                     KIYILLFVFLSVSGTLVWVLFGKSLLASLRKELKNKEIEQDK"
     CDS             2506..3225
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 56.8; E-value:
                     3.3e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg82_3"
                     /sec_met_domain="NTP_transf_3 (E-value: 6.8e-17, bitscore:
                     52.2, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRTAMIMAAGMGTRFGQYTELIPKGFVKVGGIPMIVRSIDTLLS
                     CGIERIVIGTGYKQEVYEELKTDYPMLETCFSPRYAETNSMYTLYNTREILGNDDFLLL
                     ESDLIFEKQAIMSLLECPAADAMLITPVTKFQDQYYVEHDDNFRLSSCSVNKNKLNAKG
                     ELVGIHKLSGSFYKIMCADYASIVDEQPNLGYEYELLRISCSQSPVYVHKVEGLKWYEI
                     DDISDLEYAEKYIVPYC"
     CDS             3246..4547
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 207.4;
                     E-value: 4.4e-63)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg82_4"
                     /sec_met_domain="CTP_transf_like (E-value: 2.3e-13,
                     bitscore: 40.6, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 6.1e-121, bitscore:
                     392.9, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 2.9e-63, bitscore:
                     203.5, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKTVYIGMTADIMHPGLIRIINEATKYGDVIIGLLTDKAIAEHK
                     RLPYLTYEQRKEVVQNIKGVCKVVPQEEWSYVENLKRIKPDYIIHGDDWKTGPLREERV
                     RVFEVMNEQGGKVIEIPYTLGINSSSLDKDIKAIGTTPDVRLKSLRRLINAKPVVRILE
                     AHDGLCGLIIENQEILKGDKREVFDGMWSSSLTDSTSKGKPDIEAVDLTTRLQDLNNIL
                     ECTTKPIIFDGDTGGKIEHFVFTVRTLERHGISAIIIEDKVGLKKNSLFGTDVIQTQDT
                     IEGFCNKIKEGKASQITDDFMIIARIESLIAGKPVSDALERAFAYVQAGADGIMIHSKN
                     KSGEDIKEFCLAFRKQYAHVPIVVVPTTYDHIYESELCDWGVNIIIYANHMLRAAYPAM
                     MNVAKTILENERALEVRELCMPIKEILELIPGTK"
     CDS             4564..5688
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 58.1;
                     E-value: 1.1e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg82_5"
                     /sec_met_domain="TPP_enzyme_N (E-value: 1.3e-20, bitscore:
                     63.7, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.1e-20, bitscore:
                     64.0, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLSVEKVYETFLSHDVDFFTGVPDSLLKNICAYITDHTSREKHII
                     AANEGAAVGIASGYYMASGKVPVVYMQNSGLGNTVNPLLSLADEQVYSFPMLLMIGWRG
                     EPGTKDEPQHKKQGEVTLDLLKAMRIPYIILDSNENDAFIQLHDIIQSAKTKNIPHAII
                     IRKDVFGKYKLQKEFGCNYPLSREDALQIVVDYLPENSVVVSTTGKLSRELFEYREMKE
                     QGHEHDFLTVGSMGHSSSISLGIAIAKQDRPVYCLDGDGAFIMHLGAISNIGDLSPKNY
                     YHILFNNGAHESVGGQPTLGFSLDIPAIVRGSGYKHTYTVCTKVEIEEAMKQLPKLCGP
                     VLLEIKVKIDSRENLGRPTTTPIENKEHFMDFLK"
     CDS             5698..6819
                     /NRPS_PKS="Domain: Aminotran_5 (30-366). E-value: 2.9e-34.
                     Score: 110.4. Matches aSDomain:
                     nrpspksdomains_ctg82_6_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 76.3; E-value:
                     3.6e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg82_6"
                     /sec_met_domain="Aminotran_5 (E-value: 1.8e-31, bitscore:
                     99.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIERKILLNPGPATTTDSVKMAQVVPDICPREKEFAGMMKQLRD
                     DLVRVAHGNLEKHTAVLFCGSGTINIDICLNSLLPEDKRVLIVNNGAYSTRAVEVCQYY
                     GLPHINLEFSVYERPDLSVVENVLQENPDIAMVYTTHHETGTGILNPIREMGAIAHKYQ
                     AKFVVDTTSSLGMIPFDIEKDNVDFCMASAQKGLQAMTGLSFIIGNEEQIKFSKKYPKR
                     SYYCNLYLQYENFERTGEMHFTPPVQTIYATRQALNEYFEVGEEAKFARHKRVFEAIHS
                     GINEIGLKSVIKREWQSGLVVSVQYPEDPNWDFEKIHDYCYERGFTIYPGKISTEDTFR
                     ICALGEIEVDDIENFFKVLKAALNHYNVTLPMS"
     aSDomain        5788..6795
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg82_6_Aminotran_5.1"
                     /evalue="2.90E-34"
                     /label="ctg82_6_Aminotran_5.1"
                     /locus_tag="ctg82_6"
                     /protein_end="366"
                     /protein_start="30"
                     /score="110.4"
                     /tool="antismash"
                     /translation="PREKEFAGMMKQLRDDLVRVAHGNLEKHTAVLFCGSGTINIDICL
                     NSLLPEDKRVLIVNNGAYSTRAVEVCQYYGLPHINLEFSVYERPDLSVVENVLQENPDI
                     AMVYTTHHETGTGILNPIREMGAIAHKYQAKFVVDTTSSLGMIPFDIEKDNVDFCMASA
                     QKGLQAMTGLSFIIGNEEQIKFSKKYPKRSYYCNLYLQYENFERTGEMHFTPPVQTIYA
                     TRQALNEYFEVGEEAKFARHKRVFEAIHSGINEIGLKSVIKREWQSGLVVSVQYPEDPN
                     WDFEKIHDYCYERGFTIYPGKISTEDTFRICALGEIEVDDIENFFKVLKAALNHY"
     CDS             6816..7292
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1156:Chloramphenicol acetyltransferase (Score: 70.3;
                     E-value: 2.9e-21)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg82_7"
                     /transl_table=11
                     /translation="MIAEILLKIRHFYFSLRGIHFHPESVISGVPIRSFRLVGKYSNIY
                     LGKNAEIRSGCYLIARDKIIIGENSTLAYGTTILTTADPNGPYNALAILYPYKKAPVVI
                     GDNVWIGANVTILPGITIGDYVVVAAGSVVTKDVPSGVLVAGVPAVVKKQLLME"
     CDS             7361..8182
                     /locus_tag="ctg82_8"
                     /transl_table=11
                     /translation="MKDKTCHIITDDNIKEIELSILKYIDLQCKKYNLRYYLGYGTLLG
                     AVRHQGFIPWDDDIDIWMPREDYDKFISLENSLENSKFKILSPLNNSDYYYGFAKLVSR
                     NTELQEIGFLPIKDYGVYVDIFPLDNIATEHPFLHFIAKYIIMARIASCNIKPWLNLTH
                     KKKAILFRFIGIIGTLLGFRRTAKLTDKIAKIDKGKTNKRVGVFVESIGEHNIFATEYF
                     ASTIQLKFEGIYFNAPKEYRKCLTQLYGDYMQLPPENRRISGHSFKACYRL"
     CDS             8167..8925
                     /locus_tag="ctg82_9"
                     /transl_table=11
                     /translation="MLQIMKLTTYVLNLLKDKERKEYMQYVLSDYPNLDIQYVNAINGK
                     NLSLDEILNQFDNNKAYKRYGRECDLGEIGCSLSHRLAFDLLMNSESNYALILEDDIVI
                     GDGFSSVLEKLLPLIDIDAPCVILLSGGVSYYKKRYYNTLCLGVVYMGDYAHSYLINKA
                     AAKLILDDKKAYIFADDWDYIRNRGVNVLVILPHLVDQRRDGSFVSNVWQDNHHIVKVK
                     LSLIRLCNYILSRIIDKILKRMGNYENKTL"
     CDS             8927..10462
                     /locus_tag="ctg82_10"
                     /transl_table=11
                     /translation="MVESRSKKGIANIKCSLFFYIVNLFLNIISRKVFIDYLGIEVLGL
                     NTTVTNLLSFLNLAELGIGNAIAFTLYKPLFLKDFQVIREIVSVQAWLYRRIAYFISIG
                     ALGLMLLFPVIFKKIDLPLWYAYATFIVLLIASLLSYFVNYKQILLSADQKEYKITLCV
                     QGIKSLKTITQVVVILYLINGYVYWLTLELVGVLVSALLLNKIVNKEYPWLFVKVSDGK
                     KLRNKYPEIIVKTKQFFFHRISSFVLNQTSPLIIYAFASLTMVAIYGNYMLIVFGGIAL
                     VNAFFNGFNAGVGNLVAEGDKKKIKSFFWEITSFRIWIAAIICVCFFKLCHPFISLWVG
                     KEYVLDEMTFIVLTIYVFISLTRVNEAFLFAYGLYQDIWAPIIEAILNLGLSVLLGFIW
                     GLTGIISGVVISLVVIVCIWKPYFLFKMGFKDNILEYVSRYIKYYMMIALSFILSHLIL
                     NTMFIDFQSSYWKLFEYACTVGSCYAILSFLFFYLFDNGMRQFTRRLLNIFLK"
     CDS             10532..11545
                     /locus_tag="ctg82_11"
                     /transl_table=11
                     /translation="MSFYIFIFVVLLYFSFRKYNKYIYWGTILLLSLIAGLRSETIGVD
                     THAYKDIFIWISNEVLYPIEPAWYFLNKLIAWVGGSFNFLLWITALLTLIPIGVVSLRC
                     SSNPQMSLFFYYGLYAYINSFNGMRQFVAISFVLLAYSYITQKKNFFLYLLIAFIFHYS
                     AIFSFIAILVDKVSLTKRRVIGGIFSSFFVGMFFNDSMFLVLAGPYAGYLESSEGYRDN
                     FLLAAIMAILMSCLYMCTFFTLRREYKQNLWTKMFFVGIIILNITMRLELGARIILYFT
                     LCQIIFYPMYFRHNIVKNKRMVVLSILLYTSLVFMKILILGNQGEYSVYPYKTILW"
ORIGIN
        1 gtgacgttgt aacagccgaa gccatcctta accgcttcac aaacaaactg gaaatccgtg
       61 gcgctgtcta tcgtcccggt atctaccagc tcagcggtaa actgaacacg atccgcgaac
      121 ttgtccatga agctcagggt ctgaccggtg acgctttcct gaatcgtgcc gtcctctatc
      181 gccagcgtga agacctgacc agtgaagttg ttcagataga cattaagtct atcatggacg
      241 gcacttcccc aaaccttgcc ctgatgaaga atgacatcct ctacattcca agtatccatg
      301 acctggaaga ccggggtaat gtcacagttc acggtgaggt tgcccaacct gattcctatc
      361 cttatgcgga taacatgaca cttgaggatc tgatcattca ggccggtggc ttgaaagaag
      421 ccgcctctac tgtccgtatt gatatatcga gacgaatcaa gaatcctcgt agtactgcgg
      481 ataatgatac gattggtcag atgtatacct tctctttaaa ggatggtttt gtaatagatg
      541 gtcagcccgg ttttatcctt gagccttatg atcaggttta tgtccgtcgc agtccgggct
      601 atcaggcaca acagaatgta gccattgaag gtgaaatcct tttcggagga aattacgcca
      661 tgaccagccg tgaggaacgt ctttccgatt tagtgaataa agccggcggt ccgaccaatt
      721 atgcctatct gcgtggtgcg aaactgaccc gtgtggctaa cgctagtgaa aagaaacgta
      781 tgggtgatgt aattcgcctt atgagcaggc aattgggcga agccatgatt gactctttgg
      841 gtattcgtgt tgaagatact tttacggttg gaatagattt ggaaaaagcc ttgagcaatc
      901 ctaaaagtaa tgccgatctt gtcttacgtg aaggtgatgt gatctccata cctaagaata
      961 ctaatactgt aactatcaat ggtgctgtca tggttccgaa tacggtttct tatatgaagg
     1021 gaaagaatgt ggattattac ctgaatcagg ccggtggctg ttctgataat gcaaggaaga
     1081 gtaagaagtt cattgtttac atgaatggtc aggtgacgaa ggttaagggt agtggtaaga
     1141 aacagattga gccggggtgt gagattattg tgcctggtaa ggctaagaag aaagggaaca
     1201 tagctaatat tttaggttat gctacttcct tcagttcttt gggaatgatg attgcttcta
     1261 ttgctaattt gataaagaaa taaatgacct attatgattg aaaagcaatc ttatcaagaa
     1321 ccgttttctg aagtggataa aaggaaagat gatgaaatgg aaatagactt gatggctatt
     1381 ttttataaga ttattactgt tcgtggagta ctatgcaaag ctgctggagt gggagtaata
     1441 attgctatag tggtggcatt gagcattccc aagcaatata cagtaaaggt taccctttct
     1501 cctgaaatgg gtaatgcgaa gggaagtaat ggtttagccg gacttgctgc ctcgtttttg
     1561 ggagatgggg ctctaatagg ggaaagcaca gatgccttga acgcttctct ttcagcagat
     1621 attgtttctt caactccttt tttattggaa cttttagaaa tggaagtctc cgtttctaaa
     1681 aaggatgata aaatgacttt ggggtcttat ctcgatgaag agtcatctcc ttggtgggat
     1741 tatcttatag gttttccggg catagtaata ggaggagtta aatcattatt cggagaagat
     1801 acggttcctg ccagtggagg gcggcaagga actatcgaat tgacgaaaga agtaaatgag
     1861 aagattaatt ttcttaaaaa gaagattagt gcatccattg ataagaaaac cgccataacg
     1921 agcattacgg tcaccttaca aaatccgcag atcactgcgg ttatagcaga ttctgtcgtt
     1981 cataaattac aggaatatat tattgggtat cgaacttcaa aagtaaaaga agactgtgcg
     2041 tatttggaac ggttgtttaa agaacgtcag caggaatatt atgctgctca aagaaaatat
     2101 gcggattatg tagacgccca tgataatgtg gttcttcaaa gtgtacgtgc tgagcaggag
     2161 cgtttacaga acgacatgag tttggcttat cagatatata gtcaggttgc taatcagttg
     2221 caggtatcca gagccaaagt acaggaggaa aagccagtct ttgcagtagt tgagcctgcc
     2281 gttgtacctt tgaaaccttc aggtatggga ctcaaaattt atattttatt atttgtgttc
     2341 ctgtccgtat ctggtactct cgtgtgggtg ctttttggca aaagtctgct ggctagcttg
     2401 agaaaagaac tgaaaaataa ggagatagaa caggataaat gatgctaaaa cgtcgtttac
     2461 aacattctta catcaattaa attcacttac tatattatta aacgaatgat acgtacagct
     2521 atgataatgg ctgcgggcat gggaacccgt tttggccaat atacagaatt gatacctaaa
     2581 gggttcgtca aagtgggagg tattccaatg atagttcgtt ctattgatac tttgttatca
     2641 tgtggcattg agcgaattgt aatcggtaca ggatataaac aagaagttta tgaagaattg
     2701 aagacagact atcctatgct tgaaacctgt tttagtcctc gttatgctga aacaaatagt
     2761 atgtatactt tatataatac gcgggagatt ctgggcaatg atgatttttt attgttagag
     2821 tcagatttga tttttgagaa acaagcaata atgagcttat tggagtgtcc tgcggcagat
     2881 gcaatgctaa taactccagt gacaaagttt caagatcagt attatgtgga acatgatgat
     2941 aattttcgcc tttcttcatg ttctgtcaat aagaataaac taaacgctaa aggcgaattg
     3001 gtcggcatcc ataaactatc cggttccttt tacaaaataa tgtgtgctga ttatgcgagt
     3061 attgttgatg aacaacccaa tttgggatat gagtatgaat tactacgaat atcttgttct
     3121 caatctcctg tttatgtgca taaagtagaa gggctgaaat ggtatgaaat cgatgacata
     3181 tcagatttgg aatatgcaga gaaatatatt gttccgtatt gttaattaaa ataatatatc
     3241 aaattatgag taagacagtt tatattggga tgactgcgga tattatgcat ccaggactta
     3301 tccgcattat taatgaggct actaaatacg gtgatgttat aattggtctt cttacagata
     3361 aagctattgc ggagcataaa cgccttcctt atctgaccta tgaacaacga aaagaggtag
     3421 ttcagaatat taaaggcgta tgtaaagttg ttcctcaaga agaatggagc tatgttgaga
     3481 atctcaaacg cattaaacct gactatataa ttcatggtga cgactggaaa accgggcctt
     3541 tacgtgaaga acgtgtgcgc gtatttgaag tgatgaatga acagggagga aaggttatcg
     3601 aaattccata tactttggga attaattcat cttctttgga taaagatatt aaggctatag
     3661 gtacaacccc tgatgtacgt ttaaaatcac ttcgtcgttt gataaatgcc aaacccgtag
     3721 ttcgtatttt ggaagctcat gatggactat gtggcttgat aattgaaaat caggaaatat
     3781 taaaagggga taagagggaa gtatttgacg gaatgtggtc tagtagcctt acggattcaa
     3841 ccagtaaagg taaacctgat attgaggcgg ttgatttaac tacccgattg caagatttga
     3901 ataatattct ggagtgtact actaaaccta ttatttttga tggagataca ggtggtaaaa
     3961 ttgaacattt cgtatttaca gtacgcactt tagaacgtca tggaatttca gctataataa
     4021 ttgaagataa agttggatta aaaaagaatt cattgtttgg tacagatgtt attcaaactc
     4081 aagatacaat cgaaggtttc tgtaataaga tcaaagaagg aaaggcatct cagattactg
     4141 atgattttat gattatcgct cgtattgaga gccttatagc gggaaagccg gtaagtgatg
     4201 ctttagagag agcttttgca tatgtccaag cgggagcgga cggtattatg atacatagca
     4261 aaaataagtc gggtgaagat ataaaagaat tttgtttggc gtttcgaaaa cagtatgctc
     4321 atgtacctat cgtggttgtg cctactacat atgatcatat ttatgagtct gaattgtgtg
     4381 attggggtgt gaatataatt atttatgcta atcatatgct tcgtgccgct tatcctgcta
     4441 tgatgaatgt cgcaaagaca atattagaga atgaacgtgc tttagaggtg cgcgaactgt
     4501 gtatgcccat aaaagaaata ttggaactga ttccaggtac taaataaaaa gaggaatatc
     4561 attatgttaa gcgtagaaaa agtttatgaa acgtttctga gtcatgacgt tgattttttt
     4621 acaggagttc cagactcatt actaaaaaat atttgtgcct atatcacaga ccatacttca
     4681 cgtgagaaac atatcatagc tgctaatgaa ggtgcggctg taggaattgc ttcaggttat
     4741 tatatggcat caggtaaggt tcctgtggtt tatatgcaaa attccggctt gggtaatact
     4801 gttaatcctc tgctgtctct tgcagatgaa caagtataca gttttccaat gcttttgatg
     4861 attggctggc gtggtgaacc gggaacaaaa gatgaacctc agcataaaaa acagggtgag
     4921 gtaactttag atttgttgaa agcaatgcgg attccttata taattcttga ttcaaatgag
     4981 aatgatgcat ttattcagct ccatgatatc atacaatcag caaaaactaa gaatattcct
     5041 catgcaataa ttattcggaa agatgttttt ggcaaatata aacttcaaaa agagtttgga
     5101 tgtaattatc ctttatctcg tgaggatgct ttgcaaatag ttgtggatta tctgcctgaa
     5161 aattcagtgg tcgtttctac tactggcaag ctttctcgtg agttatttga atatcgagaa
     5221 atgaaagagc aaggtcatga acatgatttt ctcacggtag gctctatggg acatagttca
     5281 tcaatctctt tgggtattgc tatagcaaag caagatagac ctgtttattg cttggatggt
     5341 gatggagcat ttattatgca tttaggggca attagtaaca ttggcgattt atctcctaaa
     5401 aactattatc atattttgtt taacaatggt gctcacgagt ctgtaggagg acagcctact
     5461 ttaggattta gtttagatat tccggctatt gtccggggga gcggatataa gcatacttat
     5521 actgtttgta caaaagttga aattgaagaa gcaatgaagc agcttccaaa gttatgtggt
     5581 cctgttttgt tggaaataaa ggttaaaata gattctcggg agaatttggg acgtcccacg
     5641 actactccta tagagaataa ggagcatttt atggattttt taaaataaat atattcgatg
     5701 aaaatagaaa gaaaaatatt attaaatcct gggcctgcca ctactactga cagtgtaaaa
     5761 atggcacaag ttgttccgga tatttgtcct cgcgagaaag aattcgcagg tatgatgaaa
     5821 caacttcggg acgatttggt cagagttgct catgggaatt tggaaaaaca taccgctgta
     5881 ttattttgtg gctcaggaac catcaatatt gatatatgtc taaattctct tcttcctgaa
     5941 gacaaaaggg tgcttatagt gaataatggg gcatatagta cccgtgctgt agaagtgtgt
     6001 caatattatg gtttgcctca tattaatttg gaattttccg tttatgaacg tcctgattta
     6061 tcggtggtgg aaaatgtgtt gcaagaaaat cctgatatag ctatggttta tactactcat
     6121 cacgagacgg gtacaggaat tctgaaccct attcgcgaga tgggcgctat agctcataaa
     6181 tatcaggcta aatttgtcgt cgatactact tcttctttgg gcatgattcc ttttgatata
     6241 gagaaagata atgtggattt ttgtatggca tctgctcaaa aaggacttca agcgatgaca
     6301 ggtctttctt ttattatagg caatgaggaa cagattaagt tttcaaaaaa atatcccaag
     6361 cgttcttact attgtaatct ttatttgcag tatgagaatt ttgagagaac aggtgaaatg
     6421 cattttactc caccggtaca aactatttac gccactcgtc aggcattaaa tgaatatttt
     6481 gaagtgggcg aagaagctaa gtttgcccgt cacaaaagag tttttgaggc tattcattca
     6541 ggtataaatg aaattggtct taaaagtgta ataaagcgtg aatggcagtc aggactggtt
     6601 gtctcagttc aatacccaga agatcccaac tgggattttg agaaaataca tgattattgt
     6661 tatgagcgtg gatttacgat ttatccaggt aagatatcaa ctgaggatac ttttagaatc
     6721 tgtgctttag gagagataga agtagatgat attgagaact tctttaaagt actaaaagct
     6781 gcattaaatc attataacgt cacattaccg atgtcatgat tgcagaaata ttattgaaga
     6841 ttcgtcattt ttatttttcc cttcgtggta tacacttcca tccggaaagt gttatttctg
     6901 gagtgcctat tcgtagcttt cgcttagtag gcaaatatag caatatctat ttgggtaaaa
     6961 atgctgaaat acgttcaggc tgttatctga ttgctcgtga taaaatcatt attggagaaa
     7021 actcaacact cgcctatgga acgactatat tgacaactgc tgatcctaat ggaccgtata
     7081 atgcattagc aattctttat ccttataaaa aggcaccggt agttatagga gataatgttt
     7141 ggattggggc taatgttacc atccttcctg gaataactat tggtgattac gttgtagtag
     7201 cagctggatc tgttgtaaca aaagatgtac cttcaggggt attggttgct ggtgtaccag
     7261 ctgttgtgaa aaaacagctt ttgatggaat aagaataatt gttttattct tattgtcaga
     7321 caatatcatt acataagaag tataatttaa tgcctcaata atgaaagata aaacttgtca
     7381 tataataaca gatgataata taaaagaaat agaattatct atattgaaat atattgattt
     7441 acaatgtaaa aagtacaatc taaggtatta tttgggatat ggtactttat tgggagcagt
     7501 gcgtcatcaa ggtttcattc cttgggatga cgacattgat atctggatgc cacgggagga
     7561 ttatgacaaa tttataagtt tagaaaattc attggaaaac tcaaagttta agattttatc
     7621 tcctttgaac aattcagatt attattatgg atttgctaaa ctagtatcaa gaaatacaga
     7681 acttcaggaa ataggatttc ttcctattaa ggattatggt gtatatgtgg atatttttcc
     7741 tttggataat attgcaacgg agcatccgtt tttacatttc atagctaaat atataataat
     7801 ggcaaggatt gcgtcttgta atataaaacc ttggttaaat ctgacgcata agaaaaaagc
     7861 tattttattt cgctttatag gaataattgg gacattgtta ggatttcgaa gaacagcaaa
     7921 attaacggat aagatagcta aaatagataa gggtaaaacg aataaaaggg tgggggtctt
     7981 tgttgaaagc ataggagagc ataatatatt tgcaacagaa tattttgctt ctacaataca
     8041 gttgaaattt gagggaatat attttaatgc tccaaaggaa tatcgaaaat gtttgactca
     8101 gctttatgga gattatatgc aactacctcc cgaaaataga cgcatttctg gtcattcatt
     8161 taaagcatgt tacagattat gaaactaaca acatatgttc ttaatttatt gaaagataaa
     8221 gaacgaaaag aatatatgca atatgtattg agcgattatc ctaatctaga tattcaatat
     8281 gttaatgcaa taaatggaaa aaatcttagt ctggatgaga tattgaatca atttgataat
     8341 aataaagcat acaaacgcta tggaagagaa tgtgatttag gagaaattgg gtgcagtttg
     8401 agtcatcgat tggcgtttga tttattaatg aattcggaga gtaactatgc tttaatttta
     8461 gaagatgata ttgttattgg ggatggattt agtagcgtat tggaaaaatt attgccattg
     8521 attgatatcg atgctccttg tgttatttta ttgtcaggtg gtgtctctta ttataaaaaa
     8581 agatattata atactttgtg tttgggtgta gtttatatgg gagattatgc gcatagttat
     8641 ttaataaata aggctgctgc taaattaata ttggatgata aaaaagcgta tatatttgct
     8701 gatgattggg attatatacg taatagagga gtaaatgtac tagttatttt acctcattta
     8761 gttgatcaac gtagagatgg aagttttgta agtaatgttt ggcaagataa tcaccatatt
     8821 gtgaaggtga aattatcatt gattaggttg tgtaactata ttctttcaag gataatagat
     8881 aaaatattga agcgaatggg aaattatgag aataagacac tgtagtatgg ttgaatcacg
     8941 ttctaaaaaa ggtatagcaa atataaaatg ttctttgttt ttttatatcg taaacttatt
     9001 tcttaatatt atatctagaa aggtctttat cgattattta ggaatagaag tactaggact
     9061 taacacaaca gttactaatc tgcttagttt tctaaatttg gcagaattgg gaattggtaa
     9121 cgcaattgca tttactttat ataaaccttt atttttgaaa gattttcaag ttataaggga
     9181 gatagtgtct gttcaagctt ggctttatcg acgaattgca tattttatat ctataggtgc
     9241 tttaggacta atgttgcttt ttcctgtaat ttttaaaaaa atagatttac ctttgtggta
     9301 tgcttatgct acatttattg ttttattgat agcttctttg ttgagctatt ttgtgaatta
     9361 taaacagata cttctaagtg cagatcaaaa agaatacaaa ataacacttt gtgtacaggg
     9421 aataaaatca ttaaaaacca taacacaagt ggttgttata ttatatctaa taaatgggta
     9481 tgtgtattgg ttaactcttg aacttgtggg ggtattggtt tcagctttgc tattaaataa
     9541 aattgtaaac aaagaatatc cttggttgtt tgtaaaggtc tctgatggaa aaaaattacg
     9601 taataaatat ccagaaataa tagtaaagac aaaacagttt ttttttcatc gcatctcatc
     9661 atttgtttta aatcaaacta gtccattgat aatctatgcc tttgctagtc ttacaatggt
     9721 agcaatctat ggaaattata tgttgatagt ttttggtgga atagctctgg taaatgcttt
     9781 ttttaatggt ttcaatgctg gagtgggaaa tcttgttgct gaaggagata agaaaaaaat
     9841 aaaatctttt ttttgggaaa taacttcttt tcggatatgg atagcagcca tcatttgtgt
     9901 ctgttttttt aagttgtgtc atccttttat ttcattgtgg gtgggaaagg aatatgtttt
     9961 ggatgaaatg acctttatag tgttaactat ttatgttttt atatcattaa caagagtaaa
    10021 tgaagctttt ctatttgctt atggattata tcaggatata tgggctccta taattgaagc
    10081 tatattaaac ttgggacttt cggttttatt gggatttatt tggggactaa caggaataat
    10141 atctggggtt gttattagtt tagttgtcat tgtatgtata tggaaaccat attttttatt
    10201 taaaatgggt ttcaaagata atatattaga atatgtctct agatatataa aatattatat
    10261 gatgattgct ttatctttta ttttgtcaca tttaattctt aatacaatgt ttatagactt
    10321 ccaaagttca tattggaagc tttttgaata tgcttgtact gttggaagct gttatgctat
    10381 attgagtttt ctcttttttt atttatttga taatgggatg agacaattta ctagacgttt
    10441 attaaatata tttctaaaat aatttagtga taaggtgtag ttgttatatt tagtttttag
    10501 ctgaaataga ttttgtcttg tattataaaa aatgagtttt tatatattta tttttgttgt
    10561 tttgctgtat ttctctttta ggaaatataa taaatatatt tattggggaa ctattttatt
    10621 actttcattg atagcagggt tgaggtccga aacgatagga gttgatactc acgcttataa
    10681 agatatattt atatggatat caaatgaagt attatatccg atagaacctg catggtattt
    10741 tttaaataaa ttgattgctt gggtgggggg aagttttaat ttcttgttgt ggataactgc
    10801 actattgact ttaataccaa taggtgtagt tagtttacga tgtagcagta atcctcagat
    10861 gagtttgttt ttttactatg gattatatgc atatattaat tcatttaatg gaatgagaca
    10921 gtttgttgct atttcatttg tattattagc ttattcttat atcacacaaa agaagaattt
    10981 ttttttgtat ttactaattg cattcatatt ccattattca gcgatttttt ctttcattgc
    11041 tattttggta gataaggtat cattaactaa acgacgtgta attggtggta ttttttctag
    11101 tttttttgta ggtatgtttt ttaatgattc tatgtttcta gttttggctg gtccttatgc
    11161 aggttattta gaatcttctg agggatatag ggataatttt ttattggctg ctataatggc
    11221 aattttgatg agttgtttat atatgtgtac gttttttact ttacgaagag aatataaaca
    11281 aaatttgtgg acaaaaatgt tttttgttgg cattattata ttgaatataa ctatgcggtt
    11341 agaattagga gctcgtatta ttttgtattt cacattatgc caaattatat tttacccaat
    11401 gtatttccgt cataatattg tcaaaaataa aagaatggtt gttttgagta ttttattgta
    11461 tacttcgttg gtttttatga aaatattaat tttgggtaat caaggagaat attctgtcta
    11521 tccttataaa acaatattat ggtaagcatt attattcctt gttacaattc gggaaaatat
    11581 atttctgaat gtttaaattc tattttagct caaacatatt ctgaatggga agcaatttgt
    11641 gttaatgatg gtagtactga taatacaatg gaatttctga atcaatatgt aatgtcggat
    11701 aatcgatata aagtagtttc tcagcttaat tcaggagtgt cagcagcaag aaataaggct
    11761 ttatctatat gcagggggga atatatttgc tttgtagata gtgatgatat agtagaacc
//
