LOCUS       c00244_gnlPro..         3786 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MBKHAFMH_244.
ACCESSION   c00244_gnlPro..
VERSION     c00244_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-03 21:39:56
            Original ID  :: gnl|Prokka|MBKHAFMH_244
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3786
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3786
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[106:3098]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      107..3098
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3786
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3786
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             107..2359
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg244_1"
                     /sec_met_domain="Gly_radical (E-value: 3.8e-43, bitscore:
                     134.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.7e-268, bitscore:
                     877.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQKEQWQGFNGRIWREEINLREFIQDNYTPYDGDASFLEGPTEA
                     TDTLWGELQKLQKAEREKGGVLDMDTDIVSSLTSHKPGYISESLKEKEQIVGLQTDKPL
                     KRAFMPFGGIRTAEEACTTYGYTPNPEFHKIFTDYHKTHNQAVFDSYTPEMKKARHNKI
                     ITGLPDTYGRGRIVGDYRRVALYGIDLLVADKQKDFANCGDGTMTDDVIRLREEISMQI
                     KALKDMKVMAESYGYDISEPASNAKEAVQWTYFGYLAAIKTQNGAAMSIGRIATFLDIY
                     IKRDMDKGILTEKEAQELIDHMTMKFRMVKFGRIPAYNQLFSGDPVWATLEVGGLGQDG
                     RSMVTKTDYRFLHTLENMGPSPEPNLTVLYSERLPKAFRDYAAHISITTSSIQYENDDV
                     MRPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKTGQQVGPEYKP
                     ITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYEYALMALIDTNVRRTFA
                     TGIAGFSHVVDSLSAIKYAKVKTVRNEEGLVVDYETTGDFPKYGNDDDRADDIAVWLLQ
                     TFMKKLEKFHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQ
                     NGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERINNLVNVMDGYFAQGAHHL
                     NVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYAVKFISLTKEQQLDVIARTCHDRM"
     CDS             2346..3098
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.8;
                     E-value: 2.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg244_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.9e-32, bitscore:
                     100.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTECNKNAMALVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNP
                     DTWKMTEENGAVWRSADELLNQALRYRTYWKNSGGITVSGGEPLLQIDFMLEFFKKAKE
                     KGIHTVIDTAGGPFTREEPFFSKFQELMKYTDLLLVDIKHIDTECHKTLTGRSNENILD
                     MIRYLSDIKKPIWIRHVLVPERSDKDEYLTRLADFIHSLDNVEKVEILPYHTMGIYKWK
                     ELGLEYPLEGIEPPTKERVENAKKILAI"
     CDS             complement(3559..3786)
                     /locus_tag="ctg244_3"
                     /transl_table=11
                     /translation="EYYLKVIDWPHNPGPSEWWPGSSLHVCEMEEREFRVIRKKYKEDD
                     GPMPCLYGCPNSRDIEEKKEVETKVEIVYF"
ORIGIN
        1 aaactaatca tagtaaatca tctgaacaat tttgttgatt ttttagcaaa catatttcaa
       61 aaccttgact taaggatttt tttaacaaac agggaggtca attaagatgt tacaaaaaga
      121 acaatggcag ggatttaatg gacgaatctg gagagaagaa attaatttaa gagagttcat
      181 ccaggataac tacactccat atgatggaga tgcttctttc ctcgaaggtc ctaccgaagc
      241 tactgacaca ctgtggggcg aactgcagaa gctgcagaaa gcagagcgtg aaaagggtgg
      301 tgttcttgat atggatactg atatcgtatc aagccttact tcccataaac cgggttacat
      361 ttctgagtct ttaaaagaga aagaacagat cgtaggtctt cagacagata aacctttaaa
      421 acgtgcattt atgccatttg gtggtatccg tactgctgaa gaagcttgta caacttatgg
      481 ttacacacca aatccagaat tccacaagat ttttacagat tatcataaga cacataacca
      541 ggcagtattt gattcttata ctcctgaaat gaagaaagct cgtcacaata agatcattac
      601 tggtcttcca gatacttacg gacgtggacg tatcgttggt gactaccgtc gtgtagctct
      661 ttacggtatt gacctgttag ttgctgacaa gcaaaaagat ttcgcaaact gcggtgacgg
      721 aacaatgaca gatgacgtta tccgtttaag agaagaaatc tccatgcaga ttaaagcatt
      781 aaaagatatg aaagttatgg ctgaaagcta tggctatgat atttccgaac ctgcttctaa
      841 cgcaaaggaa gctgttcaat ggacgtattt cggatacctt gcagcaatca agacacagaa
      901 cggtgctgct atgagtatcg gacgtatcgc tactttcctt gacatttata tcaaacgtga
      961 tatggataag ggaatcctta cagagaaaga agcgcaggaa ttaatcgacc atatgactat
     1021 gaagttccgt atggttaagt tcggacgtat cccagcttac aaccagctct tctccggtga
     1081 ccctgtatgg gcaacacttg aagttggcgg acttggacag gacggtcgtt ccatggttac
     1141 aaaaactgac taccgtttcc tccacacatt agaaaatatg ggaccatccc cagagccaaa
     1201 cctcacagta ctttactctg agcgtcttcc aaaagcattt agagattacg cagctcatat
     1261 ttctattaca acaagttcaa tccagtatga aaatgatgat gtaatgcgtc cagtatgggg
     1321 cgatgattac tccatctgct gctgtgtttc tgctacacag actggtaagg aaatgcagtt
     1381 cttcggagcc cgtgcaaacc ttgcaaaatg tctcctctac gcaatcaatg gtggtgtaga
     1441 tgaaaagact ggtcagcaag ttggtccgga atacaaacca attacttccg aatatcttga
     1501 ctatgatgaa gtaatgcaca aatatgatat catgatggac tggttagcag gtttatatgt
     1561 aaatacatta aatctcatcc agtacatgca tgacaaatat tactatgaat atgctttgat
     1621 ggccttaatc gatacaaatg tacgtcgtac attcgctaca ggtatcgcag gattctcaca
     1681 tgtagtagat tccttaagtg cgattaaata tgccaaagta aaaacagtcc gcaatgaaga
     1741 aggacttgtt gttgattatg agacaactgg cgacttccct aaatacggaa atgatgatga
     1801 ccgcgctgat gatatcgctg tatggttact tcagacattt atgaagaagc ttgaaaaatt
     1861 ccatacttac cgtgattctg aacctacaac ttccatcctt actatcacat caaacgtagt
     1921 atatggtaag gcaactggtt cccttccaga cggaagaaag gccggagaac ctctttcccc
     1981 aggagcaaac ccaagttatg gtgctgagca gaacggactt ttagcttcct taaactctgt
     2041 agcaaaactt ccttacgaat gggcattaga tggtatttcc aatacacaga caatcaaccc
     2101 tgatgcactc ggtcattccg aagaagaacg tatcaataac ttagtaaatg ttatggacgg
     2161 ctacttcgca cagggtgctc accacctaaa cgtaaatgta tttggtgttg agaaactgat
     2221 tgatgctatg gaacatccgg aaaaagaaga atacgcaaac tttacgatcc gtgtttcagg
     2281 ttatgcagtt aaattcatct ctctgacaaa agaacagcag ttagatgtaa ttgccagaac
     2341 atgccatgac agaatgtaat aaaaatgcga tggctcttgt tcattctacg gagagttttg
     2401 gttcggtgga tgggccggga gttcgtttta ttgttttttt acagggttgt cctttgcgat
     2461 gtcagttctg tcacaatcca gatacatgga agatgacaga ggaaaatggt gcggtgtgga
     2521 gaagtgcgga tgagctgtta aatcaggcac tccgttatcg tacttactgg aaaaatagcg
     2581 gcggtattac cgtaagtggt ggtgagcctc ttttacagat tgattttatg ctagagtttt
     2641 ttaaaaaggc aaaagaaaaa ggaatccaca ctgttattga tactgctgga ggacctttta
     2701 caagagagga acctttcttt tctaaatttc aggaattgat gaaatataca gacctgttat
     2761 tagtggatat caaacatatt gatactgaat gtcacaagac tttaactggt cgaagcaacg
     2821 aaaatattct cgacatgatt cgctatcttt ctgatatcaa aaaaccaatt tggattcgtc
     2881 atgtccttgt tccggaacga agcgataaag atgaatatct gactagatta gctgatttca
     2941 ttcactctct tgataatgtg gaaaaggtgg aaatcctgcc ttatcatacg atgggaattt
     3001 ataaatggaa agaacttgga ttagagtatc cactggaggg aattgagcca ccaacaaaag
     3061 aacgagtgga gaacgctaag aagattcttg ctatctgatc tatcaatttc tgatttgtaa
     3121 agttgatgac aaacgcagct gtggagatat ttatggtcgg attgcatcga tgacttctaa
     3181 cagagagatt cttctaaggt tgtatttagt tccattaatt tgcaggattt tttgcaagat
     3241 ttagagaaac agacgttaca aaaatttttt tccggatttt acaaagaata taggaaaaag
     3301 aaacataaaa catagcctat tttgcgactt tgtgctgcgg aattttgacc ataggaactt
     3361 gggactccct tctcctgcct tagcagatcg aaaaaagctc cagtggaggt tttgaggcca
     3421 agttcctgct ttactgggca aaatggagca tttagcacaa cggaacaaaa taggctattg
     3481 ttttatgttt cctatctaaa cacttctaaa aagccggcta cattgcccta aacaacggcg
     3541 tctgtttagc actacaaatc agaaataaac aatctctact tttgtttcca cttctttctt
     3601 ttcttctata tctcttgaat tcggacaacc ataaaggcaa ggcattggac catcgtcttc
     3661 tttatatttt ttcctgataa cacgaaattc tctctcttcc atttcacata catgtaaact
     3721 actacccggc caccactcac ttggaccagg attatgtggc caatctataa cttttagata
     3781 atactc
//
