LOCUS       c00323_gnlPro..         6504 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MBKHAFMH_323.
ACCESSION   c00323_gnlPro..
VERSION     c00323_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-03 21:39:56
            Original ID  :: gnl|Prokka|MBKHAFMH_323
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6504
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6504
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[119:5193]"
                     /cutoff="2000"
                     /detection_rule="(Complex1_51K and minimum(5, [FMN_bind,
                     FeS, Fer4, Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N,
                     SLBB]))"
                     /neighbourhood="10000"
                     /product="Rnf_complex"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      120..5193
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="(Complex1_51K and minimum(5, [FMN_bind,
                     FeS, Fer4, Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N,
                     SLBB]))"
                     /neighbourhood="10000"
                     /product="Rnf_complex"
                     /protocluster_number="1"
     cand_cluster    1..6504
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Complex1_51K and minimum(5, [FMN_bind,
                     FeS, Fer4, Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N,
                     SLBB]))"
                     /kind="single"
                     /product="Rnf_complex"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6504
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Rnf_complex"
                     /region_number="1"
                     /rules="(Complex1_51K and minimum(5, [FMN_bind, FeS, Fer4,
                     Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N, SLBB]))"
                     /tool="gutsmash"
     CDS             120..1433
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: RnfC_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Fer4"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: SLBB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Complex1_51K"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg323_1"
                     /sec_met_domain="Fer4 (E-value: 4.1e-08, bitscore: 21.9,
                     seeds: 528, tool: rule-based-clusters)"
                     /sec_met_domain="SLBB (E-value: 1.1e-16, bitscore: 49.5,
                     seeds: 67, tool: rule-based-clusters)"
                     /sec_met_domain="Complex1_51K (E-value: 2.3e-53, bitscore:
                     169.1, seeds: 88, tool: rule-based-clusters)"
                     /sec_met_domain="RnfC_N (E-value: 2.7e-42, bitscore: 131.9,
                     seeds: 53, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 9.4e-07, bitscore: 18.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGLLTFKGGLHPYDGKELSKDKPITEYLPQGELVYPLSQHIGAPA
                     VACVKKGDRVLVGQKIAEAGGFVSANIYSSVSGTVKKIEPRMTVSGNKVNSIIVENDGE
                     YETVSFQPVTEKTNEAIINAVKEAGIVGLGGAGFPTHVKLSPKEPEKIDTIIINAAECE
                     PYITADYRCMMEIPEQLISGLNIMLSLFPKAKGVIGIEDNKPEAIAKLTEMCKNESRIE
                     VAALKTKYPQGAERSLIFAVTGRAINSSMLPADAGCIVDNVATAIAIHEAITLGKPLFE
                     RVVTVTGDAIKNPGNFKVKNGTNAAELVEAAGGFKSQPEKVISGGPMMGMALSTLDVPC
                     AKTFSSLLCFTKDEVAACEPSNCIRCGRCISVCPAGLMPTKLSEIADHGDFALFDELNG
                     CECVECGCCSYICPAKRRLTQSMKTGRREAMALRRKKK"
     CDS             1454..2407
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: NQR2_RnfD_RnfE"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg323_2"
                     /sec_met_domain="NQR2_RnfD_RnfE (E-value: 2.7e-123,
                     bitscore: 399.8, seeds: 472, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNEQLYNVSANPHVRDKASTHSIMLDVIIALLPATLFGFWNFGVK
                     AIINTVICVAVCVLAEYVWQRCMHQKITIKDCSAALTGLLLALNLPPEVPFWIPVIGGL
                     FAIIVVKQLFGGLGQNFMNPALGARCFLLISFAGIMTNFSYNGFGGSFDATTSATPLAA
                     LKAGEVVNLKAMFLGNTAGTIGETSALLLLVGGVYLIAKKIISWRIPVCYIVTLGLFVL
                     LFGGHGFDMTYLAEQLCGGGLMLGAFFMATDYVTSPITPKGQIVFGILLGILTGIFRLF
                     GGSAEGVSYAIIFCNLLVPLIERVTTPTAFGKGGKK"
     CDS             2407..3036
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: FMN_bind"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg323_3"
                     /sec_met_domain="FMN_bind (E-value: 1.6e-23, bitscore:
                     72.1, seeds: 891, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSLVKDALKLVIITVVAGLVLGAVYGITKKPIADQEAKAQMEAY
                     KAVFPKASDFKDVDGFSEEAASKVIASYKNTVDGHESDVISSAVEAVDASGKALGYIFN
                     ITTSKGYGGDIQLTVGIQSDGTVSGYSVLSISETAGLGMKAKDDPSWGKQFAGKKADAF
                     SVVKDGSGSGDDAKIDAISGATITSKAVTGAMNSCLAYFQSLEGGN"
     CDS             3038..3796
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Rnf-Nqr"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg323_4"
                     /sec_met_domain="Rnf-Nqr (E-value: 6.6e-72, bitscore:
                     230.1, seeds: 650, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNNSALERLYNGIIKENPTLVLILGMCPTLAVTTSAINGAGMGL
                     STTVVLMFSNMIISILRNFIPDRVRIPGYIVIIASLVTVVQFLLQGYVPALNDALGVYI
                     PLIVVNCIILGRAESYASKNGPVSSFFDGLGMGLGFTVSLTILGAFRELLGAGTIFGKT
                     ILSESFYTPITIFILAPGAFFVLACLVAARNKIMNRKVKNGEMEEIPAGCGDCSACGNS
                     CCSSKSFFDMSVDNKKEETKAASEEKAENK"
     CDS             3811..4386
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Rnf-Nqr"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg323_5"
                     /sec_met_domain="Rnf-Nqr (E-value: 6.4e-67, bitscore:
                     213.8, seeds: 650, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKELILLIISAAIVNNVVLSQFLGLCPFLGVSKKVETAGGMGAAV
                     IFVITIASLVTSLIYKFILAPLDLTYLQTIVFILVIAALVQFVEMFLKKSMPALYESLG
                     VYLPLITTNCAVLGVALNSVQYGYNILQSVVYGLGISIGFTIAIVILAGIREKMEYNDI
                     PESWQGMPIVMVTAGLMSIAFFGFSGII"
     CDS             4402..5193
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: FeS"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Fer4_9"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Fer4"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1020:4Fe-4S binding domain (Score: 31.4; E-value:
                     3.8e-10)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg323_6"
                     /sec_met_domain="Fer4_9 (E-value: 2e-11, bitscore: 32.9,
                     seeds: 61, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4 (E-value: 1.8e-09, bitscore: 26.2,
                     seeds: 528, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_6 (E-value: 2.5e-09, bitscore: 26.0,
                     seeds: 134, tool: rule-based-clusters)"
                     /sec_met_domain="FeS (E-value: 7.4e-16, bitscore: 46.6,
                     seeds: 364, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.1e-07, bitscore: 21.1,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSISGIILAAVVVGGTGLVISILLGIASEKFKVPVDEKEVAVREC
                     LPGNNCGGCGFAGCDALAKAIAAGEAPVGACPVGGQPVADKIASIMGVEAGAGEKQVAF
                     VKCAGTCDKAKSKYKYAGNEDCVSAMSVPGGGPKACSFGCTGFGSCVKVCDFDAIHVIN
                     GVAVVDKEKCVACGKCVATCPKSLIELVPYAAPHKVQCNSKEFGKAVKEVCSAGCIGCK
                     MCTRVCEADAITVENNIAKIDYSKCTGCGKCAEKCPAKIIL"
     CDS             5503..6504
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Hydrolase_4"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg323_7"
                     /sec_met_domain="Hydrolase_4 (E-value: 1.3e-32, bitscore:
                     101.8, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAYQKFTGTYQSSDHINRIHYYIYEPETDLRAILQIVHDFGDFVE
                     RNENLIRFFTDHGVMVCGCDHIGHGRSSKKEDYGYFGSKNGWTYLVKNTKKLTHYMKRE
                     YPDTPYFIYGHGLGSLIVRMDCIHEKGINGVILSGTSGKQKYCWRKILLAALLKRIWGA
                     EHRSVYLEKDIEKRLNHRFLKEQDTYSWIAANEKIRREYSRIYSEHLPVTVAAYEDILK
                     MLALVSTRKWYRSVNEDIPILLLGGKEDPIGNGGKGILEVHRQLEDTRHWVELHLYEGM
                     RHNICDEVRRDEVYTDMLQWMKLHMNEEYEPVSYKHIRPNETYIQIECRILLE"
ORIGIN
        1 atattattat taatgatttt atatcaaata ttgaaatgct ttcgtaagaa tttatgtgag
       61 acttacaaaa atatgcatgc actatttgat ttaaaaaatt ttagaaagaa ggtaaaaaaa
      121 tgggactact aacatttaaa ggtggccttc atccgtatga tggtaaagag ttaagtaagg
      181 acaaaccaat tactgaatat ttaccacagg gagagctggt atacccttta agtcagcaca
      241 ttggtgctcc tgccgttgcc tgtgtaaaga aaggtgacag agttttagtc ggtcagaaaa
      301 tcgcggaggc tggaggattt gtttctgcca acatctatag ctctgtttct ggaacggtaa
      361 aaaagattga accaagaatg acagtttctg gtaacaaagt gaactccatt attgtagaga
      421 atgacggaga gtacgaaact gtttccttcc aaccagtaac agaaaagaca aatgaagcaa
      481 tcatcaatgc agtaaaagaa gcaggtatcg ttggactcgg tggtgccgga ttccctacgc
      541 atgtaaagct ttccccaaag gaaccggaaa aaattgatac aattattatc aacgcagcag
      601 aatgtgagcc atacatcact gccgattatc gttgtatgat ggagattccg gaacagctta
      661 tttctggtct taatattatg ctttccttat tccctaaagc taagggtgta atcggaatcg
      721 aagataataa gccagaggcg attgcaaagt tgacagaaat gtgtaaaaat gagagccgta
      781 ttgaagtagc tgcattaaag acaaagtatc cacagggtgc agagcgttct ttaatatttg
      841 cagtaacagg tcgtgccatt aattcttcca tgcttccagc cgatgccgga tgtattgtag
      901 ataacgtggc aactgcgatt gcgattcatg aggcaattac acttggaaaa cccctctttg
      961 agagagtagt aactgtaaca ggagatgcga ttaagaatcc tggaaacttt aaagtaaaga
     1021 acggaacaaa tgctgcagaa ttagtagaag cagcaggcgg atttaagtct cagccagaaa
     1081 aagtaatctc tggcgggcct atgatgggta tggctttatc tacattagat gttccttgtg
     1141 caaagacatt ctcttctctt ttatgtttca caaaagatga agtggctgct tgtgagccaa
     1201 gtaactgtat ccgttgcgga cgttgtattt ccgtatgtcc tgccggcctg atgcctacaa
     1261 agctttccga aatcgcagac cacggagatt tcgcattatt tgatgaactt aatggatgtg
     1321 aatgtgtgga atgtggatgc tgttcttaca tctgcccggc aaagagaaga ttaacccagt
     1381 ctatgaagac aggtcgtcgt gaggcgatgg ctcttcgcag aaagaagaaa taaacataag
     1441 gagaggtgaa aaaatgaacg aacaattata taacgtatca gccaatcctc atgtaaggga
     1501 taaggcttct acacatagca ttatgcttga cgtaattatc gctcttcttc cagcaacatt
     1561 atttggtttc tggaacttcg gagtaaaggc aatcatcaat acagtaatct gtgttgctgt
     1621 ctgtgttctt gcagaatatg tatggcagcg ctgcatgcac cagaagatta caattaaaga
     1681 ctgcagtgca gcacttactg gtttattatt agcattaaac cttccgccag aagtaccatt
     1741 ctggattcct gttatcggtg gtttattcgc aatcatcgtt gtaaaacagc tttttggtgg
     1801 tttaggccag aactttatga accctgcgct tggagcacgt tgcttcttat taatttcttt
     1861 tgcaggaatt atgaccaact tctcctataa tggatttggt ggaagttttg atgcgacaac
     1921 aagtgccaca cctcttgctg cattaaaagc aggagaagtc gtaaacttaa aggctatgtt
     1981 ccttggtaat actgctggta caattggtga gacatctgca ctcttactgc ttgtcggtgg
     2041 tgtatattta attgcaaaga aaatcattag ctggagaatt ccagtatgtt atattgttac
     2101 acttggtctt tttgttctcc ttttcggtgg acatggattt gatatgacat atcttgcaga
     2161 acagttatgt ggtggtggat taatgcttgg tgcgttcttc atggctacag attacgttac
     2221 atctccaatt acaccaaagg gacagattgt atttggtatc ttacttggta tcctgacagg
     2281 aatcttccgt ttattcggtg gttccgcaga gggagtttcc tatgcgatta tcttctgtaa
     2341 cctgttagta ccattaattg aaagagttac aactcctacc gcatttggaa aaggaggtaa
     2401 gaaataatga attctttagt aaaagatgct ttaaagcttg ttattattac tgttgttgca
     2461 ggtcttgtcc ttggtgcagt atacggaatc acaaagaaac ctattgctga tcaggaagca
     2521 aaggcacaga tggaagcata caaggctgta tttccaaagg caagtgattt caaagatgtt
     2581 gacggattct ccgaggaagc agcttctaaa gtgattgctt cttataagaa tactgttgat
     2641 ggacatgaga gcgatgttat ctcttctgca gtagaggcag tagatgcttc tggcaaagca
     2701 cttggttaca ttttcaatat cacaacttct aaaggatacg gtggagacat tcagcttacc
     2761 gttggtattc agtctgacgg aacagtaagc ggatattccg tactctctat cagtgagaca
     2821 gcaggtcttg gtatgaaagc caaagatgac ccaagctggg gcaaacagtt tgccggtaag
     2881 aaagcagatg ctttctctgt tgtaaaagat ggttccggtt ctggagatga cgcaaagatt
     2941 gatgccattt ccggtgcaac aatcacaagt aaagcagtta caggtgctat gaatagctgt
     3001 cttgcttatt tccagtcatt agaaggaggt aactaagatg aaaaataata gtgctttaga
     3061 aagactttat aatggtatta tcaaagagaa ccctacctta gttcttatct tgggtatgtg
     3121 tcctactctt gcggtaacaa catcggcaat caatggtgca ggtatgggac tttctacaac
     3181 cgtagtatta atgttctcca atatgattat ttctattctg cgtaatttta ttccggatag
     3241 agttcgtatt cctggataca ttgtaattat cgcatcctta gttacagtag tacagttcct
     3301 cttacagggt tatgtgccag cattaaatga tgctcttggt gtatatatcc cattaatcgt
     3361 agttaactgt attattttag gacgtgcaga atcctatgca agtaaaaatg gtcctgtttc
     3421 ttctttcttt gatggacttg gaatgggtct tggatttaca gtatccctta ctattttagg
     3481 tgcgttccgt gagttacttg gtgccggaac aatttttgga aagacaatct tatctgaatc
     3541 tttctacaca ccaattacta tttttattct tgctcctggt gctttctttg tacttgcctg
     3601 cttagtagct gcaagaaata agattatgaa ccgtaaggta aagaatggcg agatggaaga
     3661 gattcctgca ggatgtggcg attgctctgc ctgcggtaat tcctgctgtt cgagcaagag
     3721 tttctttgat atgtctgtag acaataaaaa agaagaaaca aaggctgctt ctgaagaaaa
     3781 agcagagaat aaatagaaag gggaaggatt atgaaagaat taatattatt aattatcagt
     3841 gccgctattg ttaacaatgt agtactgagt cagtttttag gattatgtcc tttcctcgga
     3901 gtatccaaaa aggtagagac tgccggtgga atgggagcag ccgtaatctt cgttattaca
     3961 attgcttctt tagtgacatc attaatttac aagtttattc ttgcaccact tgatttaact
     4021 tatttacaga cgattgtatt catcctcgtt atcgctgcgc ttgtacagtt cgtagagatg
     4081 ttcttaaaga aatctatgcc agctttgtat gaatcattag gagtatatct tccactgatt
     4141 acaacaaact gtgccgtact tggtgtagct ttaaactctg tacagtatgg atataatatt
     4201 ttacagtctg tagtttatgg tctcggtatt tctattggat ttacaatcgc gattgttatc
     4261 cttgccggaa tccgtgagaa aatggaatac aacgacattc cagagtcctg gcagggtatg
     4321 cctatcgtaa tggtaacagc cggacttatg tctattgcat tctttggttt ttcaggaatc
     4381 atttaattag gagggacaaa aatgagtata agtggaatta tccttgcggc agtagttgtc
     4441 ggtggaaccg gacttgttat ttccattctc cttggcattg cttccgagaa atttaaagtg
     4501 ccggtagacg aaaaagaagt tgctgtaaga gagtgccttc cgggaaacaa ctgtggtggt
     4561 tgtggatttg ccggttgtga tgcacttgct aaagcaatcg ctgctggaga agctccagtt
     4621 ggagcatgtc ctgtaggtgg acagccagta gcagataaaa tcgcttctat catgggtgta
     4681 gaagctggag caggcgaaaa acaggttgca ttcgtaaaat gtgccggaac ctgcgataag
     4741 gcaaaatcaa aatacaaata tgctggtaat gaagactgtg tttctgccat gagtgtacct
     4801 ggcggtggac caaaagcctg cagctttggc tgtactggtt ttggaagctg tgtaaaggtt
     4861 tgtgactttg atgctattca cgttatcaat ggcgttgctg tagttgataa agaaaaatgt
     4921 gttgcctgcg gtaaatgtgt ggcaacttgt ccgaaatctt taatcgagct tgttccatat
     4981 gcagctcctc ataaagtaca atgcaactcc aaagaatttg gtaaagcagt aaaagaagta
     5041 tgttccgcag gatgtatcgg ttgtaagatg tgtactcgtg tctgtgaagc agatgcgatc
     5101 actgttgaga acaacatcgc aaaaattgat tacagcaaat gtaccggatg tggaaagtgc
     5161 gcagagaagt gtcctgctaa gattattctt taatctgtat gatttagttg atgctaaatt
     5221 caagaatgcc ccggcattct tgaatttacg gagaagattt tttcttcttc tcatatgtct
     5281 ttgaaaatac ttgttgcaag ttatgaaata taggttgcta tagtaatttt tcagccatat
     5341 tctggaatat agtttattgt aagaaactta ataaaaacga aagaaaaggc aatctatctt
     5401 aacgaaaatg caagacagat tgtctttctt ttttcacatt tatcctgtat agtagttgag
     5461 aaaaggaatc tgtcatatac agagaggacg ggaggctttt gtatggctta tcagaaattt
     5521 acaggaacat atcaaagttc tgatcatata aacagaatac attattatat ctatgaaccg
     5581 gagacagatc ttcgtgctat tttacagatt gtgcatgatt ttggagattt tgtggagaga
     5641 aatgagaatt tgattcgttt ttttacagat catggcgtta tggtttgcgg atgtgaccat
     5701 atcgggcatg gacgttcttc taaaaaagag gactatggct attttggcag caagaatgga
     5761 tggacttatc tggtaaaaaa tacaaaaaag ctgacacact atatgaaaag agaatatcca
     5821 gatacaccgt attttatcta tggacacgga ctgggatctc tcattgtgcg aatggattgt
     5881 atccatgaaa aaggcattaa tggagtgatt ttgtcgggga ctagcggtaa acaaaagtat
     5941 tgttggagaa agattctttt agcggccctt ttgaagagga tttggggggc ggaacacaga
     6001 agtgtatatc ttgaaaaaga tatagaaaaa cgtttaaatc atcgtttttt aaaggaacag
     6061 gacacatatt cctggattgc tgcgaatgaa aaaataagac gagaatattc caggatttat
     6121 tctgaacatc ttcctgttac cgtagcagca tatgaagata ttttaaaaat gcttgcatta
     6181 gtctccacca ggaaatggta tcgttccgta aatgaagata taccgatttt gttgcttggc
     6241 ggaaaagagg atcctattgg taatggcgga aaggggatat tggaagtaca ccggcagtta
     6301 gaagacactc gtcattgggt ggagctgcat ttatatgaag ggatgcgtca taatatttgt
     6361 gatgaagtgc gaagagatga agtatataca gatatgcttc agtggatgaa attgcatatg
     6421 aatgaggaat atgaacctgt ctcttataaa catatccgac ccaacgagac ctatatacag
     6481 atcgaatgcc gtattctgct tgaa
//
