LOCUS       c00299_gnlPro..         3859 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KEDLBAPG_299.
ACCESSION   c00299_gnlPro..
VERSION     c00299_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 01:03:44
            Original ID  :: gnl|Prokka|KEDLBAPG_299
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3859
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3859
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[594:3684]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      595..3684
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3859
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3859
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..142
                     /locus_tag="ctg299_1"
                     /transl_table=11
                     /translation="TLIDMCSKLPQNEDEMLNVSGVGQNKLKKYGQRFLQEISAFQSAN
                     H"
     CDS             complement(595..1344)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 81; E-value:
                     3.3e-25)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg299_2"
                     /sec_met_domain="Radical_SAM (E-value: 8.1e-31, bitscore:
                     95.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNAKELEMLKGYVHSLESFGSVDGPGVRYVIFLSGCAMRCQFCHN
                     PDTWNMTDGKEYTTDQLLKTALRFKSYWGDKGGITVSGGEPLLQMDFLTELFRKAKEAG
                     VHTTLDTSGNPYTTKEPWHSKWLELMKYTDLLLLDIKQINEQEHVKLTGQTNTNILAMA
                     RELSDMGKPVWIRHVLVPGGSDKDEYLHQLADFIHTLKNVERVEVLPYHTLGIFKWEKL
                     GIPYPLEGVKPPTQERIDNARRILGAI"
     CDS             complement(1429..3684)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg299_3"
                     /sec_met_domain="Gly_radical (E-value: 2.1e-42, bitscore:
                     131.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.7e-268, bitscore:
                     879.5, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDFAQWEGFEGSRWKEQINVRDFIRHNYTPYDGDDSFLEGPTEA
                     TDKLWGKLQELQKAERANNGVLDMETKVVTGLTAYGPGYIAEDLKDLEKVVGLQTDKPL
                     KRAFMPYGGIKMAEQACEMYGYKVDPQIHDMFTKYEFKTHNQGVFDIYTPEMKRARHSH
                     ILTGLPDTYGRGRIVGDYRRVALYGIDALIAGKEKDFADSDRQGMRRYDFQLREEIADQ
                     IRALKGMKEMAKVYGYDISEPAKNAHEAFQWLYFGYLAAIKTQNGAAMSVGRISTFLDI
                     YIERDLKNGVITEKEAQELVDHMVMKFRMVKFARIESYNQLFSGDPVWATLEVAGIGVD
                     GRHMVTKNDYRFLHTLEDMGPAPEPNLTVLYSSKLPENFKKYAANISVTTSSIQYENDD
                     VMRPVWGDDYSICCCVSATETGKEMQFFGARANLAKCLLYAINGGVDEKLKTQVGPEYK
                     PITSEYLDYDEVMQKYDQMMDWLAHLYVGTLNMIHYMHDKYYYEAAEMALIDTKVERSF
                     ATGIAGFSHVVDSLSAIKYAKVKAIRDEDGITTDFQVEGDFPRYGNDDDRADEIARNLL
                     STFMDKLKHIHTYRDSKPTTSILTITSNVVYGKATGALPDGRKAGEPLAPGANPSYGAE
                     QNGLLASLNSVAKLDYEDALDGISNTQTINPEALGHSDEERTDNLVHVLDGYFNQGAHH
                     LNVNVFGKEKLIDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQLDVIARTCHDRM"
ORIGIN
        1 aacccttatc gatatgtgca gtaaacttcc tcagaatgag gatgaaatgc tcaatgtatc
       61 cggtgtagga caaaacaaac ttaagaaata cggccaacga tttttacagg aaatatccgc
      121 atttcagtct gccaatcact agacattatc ttgctattta gtgtcttggg tgagtgattg
      181 gttttcttat actttcttga caacgataga ttagaaaaac caataaagtt gcgggatacc
      241 aactacttca ccccaccccc gttgttgtcg ttactgtttc tttcttccct tttcagtaac
      301 cagcgatttt gcaatgtata ttcgcaagcc gcgcatttca ggagcggttt tagtggaggc
      361 gagatccatg acttacggag acttaggcgc gaaggctctc ctgagcgtct tagtcgcagt
      421 tagtcattag cttgccggaa cgttgcgacg cgcggcggtg aatatacatt gtgaaatcgc
      481 gtccgggata acgaccaaca cactaaatag caacttaaaa gagaccattg cgaactcatt
      541 tccagtccgc aacggcctcc ccttaaggag ttttatttac tttctctcac gctcttaaat
      601 cgccccaaga atccgtctgg cattatcaat cctctcctgc gtaggaggtt tcacgccctc
      661 cagcggataa ggaatcccca gcttttccca tttaaaaatg ccaagagtat gatacggaag
      721 aacttccacg cgctcaacgt ttttcagtgt atggataaaa tccgccagct ggtgcagata
      781 ctcatccttg tcactgcccc ccggtactaa cacatgccgg atccaaacag gcttacccat
      841 atccgaaagc tcacgggcca tggcaagaat attcgtattg gtctgcccgg taagcttcac
      901 atgctcctgc tcattgatct gcttgatatc cagaagcaga agatctgtat acttcatcag
      961 ctccagccac ttagaatgcc acggctcctt agtcgtataa ggatttccgc tggtatccaa
     1021 agtagtatgg actcccgctt cctttgcctt acggaaaagc tctgtgagaa aatccatctg
     1081 caaaagtggt tccccgccgc tcaccgtgat accaccctta tctccccagt aagacttaaa
     1141 ccgaagtgct gtcttcaaaa gctgatccgt agtgtactct tttccatctg tcatattcca
     1201 ggtatccgga ttatggcaga actgacaacg catagcacag ccagaaagaa aaatcacata
     1261 tctcactcca ggtccgtcca cagacccgaa gctctccaga gaatgaacat agccttttaa
     1321 catttctaat tcctttgcat tcatatttat aaaaaggaca tgcaacctct aacatatatt
     1381 gtattgcatg cccttttgtt tcgccgatac tattttaaaa tatcagactt acattctgtc
     1441 gtggcaggtt ctggcaataa catccagctg ctgctctctt gtcagatcga taaacttaac
     1501 tgcataaccg gaaacacgga tagtgaagtt tgcatactcc tctttttccg gatgctccat
     1561 agcatcgatc agtttctctt tgccaaatac atttacgttc aggtgatgtg caccctggtt
     1621 gaaatatccg tccagtacat gtaccagatt atctgtacgc tcttcatcac tatgtccaag
     1681 agcttccgga ttaatggtct gtgtattgga aattccatcc agtgcatcct catagtcaag
     1741 ctttgcaaca gagttcaggg aagcaagaag tccgttctgc tctgctccgt aagatgggtt
     1801 tgctccaggt gccagaggct ctccagcctt tcttccatcc ggaagagctc cggtagcttt
     1861 accataaaca acgttagaag taatggtaag aatagaagtg gttggcttgg aatcacggta
     1921 ggtatggatg tgtttcagtt tatccatgaa ggtggaaagc agatttcttg caatctcatc
     1981 tgctctgtca tcatcattac catagcgtgg gaaatcaccc tcaacctgga aatcagtggt
     2041 aattccatcc tcatcacgga tagcttttac cttcgcatac ttaatagcag acagagaatc
     2101 tactacatgt gaaaatccag caatacctgt tgcaaaagaa cgctcaacct tggtatcgat
     2161 aagcgccatc tcagctgcct catagtaata tttatcatgc atatagtgga tcatgttcag
     2221 ggttcctaca tacagatgag ccagccagtc catcatctgg tcatatttct gcataacctc
     2281 atcataatca agatactcgg aagtgatcgg cttgtactcc ggtcctacct gagtctttaa
     2341 cttctcatcc acaccaccgt tgatcgcata aaggaggcat ttcgcaaggt ttgcacgagc
     2401 tccaaagaac tgcatttcct taccggtctc agttgcagat acacagcagc agatggaata
     2461 atcatcgccc cataccggac gcataacatc atcgttctca tactggatag aacttgttgt
     2521 aacagaaata ttagcagcat atttcttgaa gttttccggc agcttggaag aataaagaac
     2581 agtaaggttt ggctccggag ccggtcccat atcctcaaga gtatgaagga aacgatagtc
     2641 attcttggta accatgtggc gtccgtcaac accaatacct gcaacctcaa gagttgccca
     2701 taccgggtca ccggagaaca gctggttgta ggactcaatt cttgcaaatt taaccatacg
     2761 gaatttcatt accatgtggt caacaagctc ctgtgcctct ttctcagtga tcacgccatt
     2821 cttaaggtct ctttcaatat agatatcaag gaatgtggag atacgtccaa cactcattgc
     2881 agcaccattc tgggtcttaa ttgctgcaag atatccaaag tacagccact ggaatgcctc
     2941 atgagcattc tttgcaggct ctgaaatatc ataaccatag acctttgcca tctctttcat
     3001 tcctttaaga gcgcggatct gatctgcgat ttcctcacga agctggaaat cataacgtct
     3061 cataccctgg cggtcgctgt ctgcaaagtc tttctccttg cctgcgatca gagcgtcaat
     3121 accgtacaga gccacacgtc tgtaatcgcc aacgatacgt ccacgtccat aagtatccgg
     3181 aagtccggta aggatgtggc tgtgacgtgc tcttttcatt tctggtgtat aaatgtcaaa
     3241 aacgccctgg ttatgtgttt tgaactcata ttttgtaaac atatcgtgga tctgaggatc
     3301 taccttgtag ccgtacattt cgcatgcctg ctcagccatt ttgattccgc cgtatggcat
     3361 aaatgcacgt ttcagaggct tgtctgtctg aagtcctaca accttctcaa gatccttcag
     3421 gtcttcagca atatatcctg gtccgtatgc ggtcaggcct gtgacaacct ttgtctccat
     3481 gtcaagcaca ccattgttgg cacgctctgc tttctgcagc tcctgcagct ttccccacag
     3541 tttgtcagtt gcctcggtcg ggccttccag gaaagaatcg tctccgtcat aaggggtata
     3601 attgtgacgg ataaaatcac gaacgttaat ttgttctttc caacgacttc cttcgaagcc
     3661 ctcccactgt gcaaaatctt tcattcttgt acctctttct ttattttata gttctccgcg
     3721 tatattgaag aattgttatt ggtttaacga ttgtagtata cgagattgtt ttttttccgt
     3781 caatcggttt ggggcatgct tttaataaag tacaatttat ctggtaaaaa atggatttcc
     3841 ctatatataa gtcctatat
//
