LOCUS       c00004_gnlPro..         3884 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HHFKEBNM_4.
ACCESSION   c00004_gnlPro..
VERSION     c00004_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-03 21:50:07
            Original ID  :: gnl|Prokka|HHFKEBNM_4
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3884
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3884
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[782:3726]"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      783..3726
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..3884
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3884
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             complement(2..538)
                     /locus_tag="ctg4_1"
                     /transl_table=11
                     /translation="MRSLAVYLKNYKKESILAPFFKFLEVVFDLIVPIVIAQIIDVGIA
                     NHDNGYIVQRFSILILMAALGLASTITAQFFAAKASVGFATELRQAVYDHVQHLSYTEL
                     DTLGTDTLITRLTDDVNQIQNGLNMGLRLLLRSPFIVLGSMGMAFTIDFKCSLVFAVAI
                     PFLFLVVFVIMFLSIP"
     CDS             783..2054
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg4_2"
                     /sec_met_domain="HGD-D (E-value: 6.8e-57, bitscore: 181.2,
                     seeds: 370, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIIETFGHYVENLTDKKPQSARRLLKTGWGAQNLKFRYMQDKRL
                     MPADRHLANLMMDTMLWPLQKPEDSVIVSIFTPCEMMQEVGLHPYNVEGFSCYLSASKA
                     ERAFLQQAENQGIAETLCSYHKTFLGAAQKGLLPKPKCIVYTNLTCDANLLTFRTLADF
                     YQVPVFAIDVPWNQTTENVQYVADQLKDLKIFLEKNTGKTISEDRLKERLACSKRTLEN
                     YKKYQQMRADRYVPSDLVTPLYAGMTNNILLGTAEEEKYTQMLLEDIKKAPAAKGKHIY
                     WMHTIPFWSDAVRQELCFSEKAQIVGCELAETCEPDFDPENPFEAMAERMVYHSLNGSA
                     LRRIEAGIRHAKQAGADGAVWFGHWGCKHTLGAAQLAKKKFEEAGLPLLILDGDGCDRS
                     HGGEGQTSTRLGAFLEMLGGAENE"
     CDS             2047..3726
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg4_3"
                     /sec_met_domain="HGD-D (E-value: 4e-18, bitscore: 53.8,
                     seeds: 370, tool: rule-based-clusters)"
                     /sec_met_domain="BcrAD_BadFG (E-value: 2.8e-66, bitscore:
                     211.4, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTYYVCKYTPIELLESLGGECENFNHMPEGFDLADQVSHPNIC
                     GFGKTLLEGVMEGKIKELVLVSCCDTIRSVYDILLESGKLDFLYILDVLHCDSACSRER
                     MAVQLKGLAKAYAQYKGTKFDAGKFRAAFHAQEKITKSHIAVLGARMGQELFEMTSKAM
                     PLPVENDTCVHNRSVGNILPPEGASFDELMDWYAGEILGQIPCMRMMDPTGRKKLYNDP
                     SVAGIIYHTVKFCDFYSFEYAEIKNHTDVPLLKIESDYTIQSSGQLLTRLEAFAESIQP
                     ETLEQTIDGDTKGEKKMGKGYFAGIDSGSTSTDVVILNKDHEIVTSIILPTGAGAAIGA
                     DRALAEALKEAGLQREDIDALVTTGYGRTAIKNGDKSITEITCHARGAHFLNPEVRTVI
                     DIGGQDSKVIRLDENGAVANFVMNDKCAAGTGRFLEMMARTMEMNLDQMSECGLEFKED
                     ITISSMCTVFAESEVVSLIAQNKATDDIVHGLNKAVAVKTAALTRRVGGEEKYMMTGGV
                     SKNKGLVKTLEEKLGTKLVISDKAQLCGALGAALFAADMADV"
ORIGIN
        1 gaggaatact gagaaacata atgacaaaga caaccagaaa cagaaacggg atcgcaacag
       61 caaaaaccag agaacattta aaatcaatgg taaatgccat gcccatggat ccaagtacga
      121 taaacggact tcgaagaaga agacgaagtc ccatatttaa tccgttctga atctgattga
      181 catcatcggt gagtcttgtg ataagagtat ctgttcccag ggtatccaac tctgtatagg
      241 acagatgctg tacatggtca taaactgcct gtcttaattc tgtggcaaag cccacgctgg
      301 ctttagcggc aaagaactga gctgtgatgg tactggcaag accaagagcc gccattagta
      361 taagaataga aaatctctgg acaatgtatc cgttgtcgtg gtttgcaata cctacatcta
      421 tgatctgtgc aatgacgata ggtacgatca ggtcaaatac aacctccaga aatttaaaaa
      481 atggcgccag gatgctttct tttttgtagt tttttaaata aactgccaat gaacgcattt
      541 taaaaattcc ttcctttcgt aaaaaatctt tcctatatta tttcttcctt tttttgaaaa
      601 ataatacatt caaaagtgta tcatttaaga cttttttgtc aagaagaatg tgcaaaaaca
      661 gatgaacttt ggagaaaatt aagcttgact tcaaaaaaag atattactaa aatattccgt
      721 agcgctattt gtacgctggc aactgataaa ttattattac cataatgagg aagagacaga
      781 ctatggagat tattgagaca tttggtcatt atgtggaaaa tctgacggat aaaaagcctc
      841 agtcagcccg ccggctgtta aaaaccggtt ggggggcgca gaatctgaag ttccgttaca
      901 tgcaggacaa gagactgatg cctgcagaca ggcatcttgc aaacctgatg atggatacca
      961 tgctgtggcc ccttcagaaa ccggaagatt ctgttattgt aagtattttt acaccctgcg
     1021 aaatgatgca ggaggtggga ttacatccct ataatgtaga aggattttcc tgctatcttt
     1081 ctgcaagcaa agcagagagg gcgtttttgc agcaggcaga gaatcaggga attgcagaga
     1141 cactgtgcag ttatcacaag acatttcttg gtgcagcgca gaaaggactt ctgccaaagc
     1201 ctaaatgtat tgtttatacc aacctgacct gcgatgctaa cctgctcacc ttccgtaccc
     1261 tggcagattt ttaccaggta cctgtatttg ccattgatgt accatggaat cagaccacag
     1321 agaatgtaca gtatgtggca gatcagttaa aggatttaaa aatatttctg gaaaaaaata
     1381 caggaaaaac aatctctgag gacagattaa aggaacgtct tgcctgcagt aagcggactc
     1441 tggagaatta caaaaaatat cagcagatgc gtgcagacag atatgtgccc tctgatctgg
     1501 tcacaccctt gtatgcagga atgacaaaca atatccttct gggaacagca gaagaagaga
     1561 agtatacaca gatgcttctt gaagatatta agaaagcacc ggcagcgaaa gggaaacaca
     1621 tttactggat gcataccatt ccattctggt cggatgcagt gcgccaggag ctttgcttca
     1681 gtgagaaagc ccagatcgta ggatgtgaac ttgcagaaac ctgtgagccg gattttgatc
     1741 ctgagaaccc ctttgaagca atggcagaaa gaatggtata tcattcactg aacggaagtg
     1801 cccttagaag gattgaagcc ggtatccgcc atgcgaaaca ggcaggagca gacggtgctg
     1861 tgtggtttgg acattgggga tgtaaacata cgcttggtgc ggcacagctt gcaaagaaga
     1921 agttcgagga agccggtctg ccccttttga tcctggacgg tgacgggtgt gatcgaagcc
     1981 atggaggaga aggacagact tccaccaggc tgggagcttt tctggaaatg ctgggaggtg
     2041 ctgagaatga ataaaactta ctatgtctgt aaatataccc caattgaact tctggaatct
     2101 cttggcgggg aatgtgagaa ctttaatcat atgccggagg gatttgacct ggccgatcag
     2161 gtttcacatc ccaatatctg cggttttgga aaaacacttc tggaaggagt catggagggg
     2221 aaaatcaagg aacttgtcct ggtgagctgc tgtgatacta tcaggagtgt ctatgacatt
     2281 ctgctggaaa gcggcaagct ggattttctc tatattttgg atgtgcttca ctgtgacagt
     2341 gcctgcagca gagagcgtat ggctgtacag ttaaaagggc tggcgaaggc ttatgcacag
     2401 tacaaaggca caaagtttga tgccggaaaa ttccgggctg ctttccatgc acaggagaaa
     2461 atcacaaagt cccatattgc agtgcttggc gccagaatgg ggcaggaatt atttgaaatg
     2521 accagcaaag ctatgcctct tcctgtagag aatgatacct gcgtgcataa ccgttctgtg
     2581 ggaaatatac ttccaccgga gggtgcttct tttgatgagc tgatggactg gtatgcaggt
     2641 gagattctgg ggcagattcc atgtatgcgt atgatggacc cgacaggtcg taagaagctt
     2701 tataatgatc cgtctgttgc aggaattatt tatcatacag tgaagttttg tgatttttac
     2761 agcttcgaat atgctgaaat caagaatcat acagatgtac ctcttttaaa aatcgaatca
     2821 gattatacta ttcagagcag cggtcagctt cttaccagac tggaagcttt tgcagagagc
     2881 attcagcctg aaacactgga gcagaccata gatggagaca caaaaggaga gaaaaaaatg
     2941 ggaaaaggtt atttcgcagg gattgacagt ggttccacca gtacagatgt ggtgatttta
     3001 aataaagatc atgagattgt gaccagtatt atcctgccta caggagcagg agctgctatc
     3061 ggagcagacc gtgcccttgc agaggcattg aaagaggccg gattgcagag agaagatatt
     3121 gatgcgctgg ttactaccgg atacggacgt acagctatta aaaatggtga taagagtatc
     3181 acagagatca cctgtcatgc aagaggtgcg catttcctga atccggaagt ccgtactgtt
     3241 attgatattg gcggacagga cagcaaggtg atccgactgg atgagaacgg tgcagttgca
     3301 aactttgtta tgaatgacaa atgtgcggca ggaaccggac gatttctgga aatgatggca
     3361 cgtaccatgg aaatgaatct ggatcagatg agcgaatgtg ggctggaatt caaagaagat
     3421 attacaattt ccagtatgtg tacagtattt gcagaatcag aagttgtatc cctgatcgcc
     3481 cagaataagg caacagatga tattgtccat ggcttgaata aggcggttgc cgtaaagact
     3541 gccgccctta ccagacgtgt gggcggtgag gagaaatata tgatgaccgg tggtgtttct
     3601 aagaataaag gcctggtaaa gacactggaa gagaagcttg gaacaaagct ggtgatcagt
     3661 gacaaggctc agctttgcgg tgcacttggc gcagcactgt ttgcggctga tatggctgat
     3721 gtgtaaattt ttagttactg ttcactccgt tctcagcaac acgcttcgcg atgcaggaaa
     3781 tatgaacctc cttgtgcaat gcccaaggag tatcgtttgc ttcgcaaact taatttatgc
     3841 taattgcata aattcgcgcg gtatgtctcg ggttttctgt gacc
//
